Russo, T. et al. Fe65 and the protein network centered around the cytosolic domain of the Alzheimer's beta-amyloid precursor protein. FEBS Lett. 434, 1-7

Dipartimento di Biochimica e Biotecnologie Mediche, Università degli Studi di Napoli Federico II, Naples, Italy.
FEBS Letters (Impact Factor: 3.17). 09/1998; 434(1-2):1-7. DOI: 10.1016/S0014-5793(98)00941-7
Source: PubMed


A distinctive tract of all the forms of Alzheimer's disease is the extracellular deposition of a 40-42/43 amino acid-long peptide derived from the so-called beta-amyloid precursor protein (APP). This is a membrane protein of unknown function, whose short cytosolic domain has been recently demonstrated to interact with several proteins. One of these proteins, named Fe65, has the characteristics of an adaptor protein; in fact, it possesses three protein-protein interaction domains: a WW domain and two PID/PTB domains. The interaction with APP requires the most C-terminal PID/PTB domain, whereas the WW domain is responsible for the interaction with various proteins, one of which was demonstrated to be the mammalian homolog of the Drosophila enabled protein (Mena), which in turn interacts with the cytoskeleton. The second PID/PTB domain of Fe65 binds to the CP2/LSF/LBP1 protein, which is an already known transcription factor. The other proteins interacting with the cytosolic domain of APP are the G(o) heterotrimeric protein, APP-BP1 and X11. The latter interacts with APP through a PID/PTB domain and possesses two other protein-protein interaction domains. The small size of the APP cytodomain and the overlapping of its regions involved in the binding of Fe65 and X11 suggest the existence of competitive mechanisms regulating the binding of the various ligands to this cytosolic domain. In this short review the possible functional roles of this complex protein network and its involvement in the generation of Alzheimer's phenotype are discussed.

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Available from: Paola de Candia, Jun 10, 2015
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    • "FE65 which was originally identified as an EST corresponding to an mRNA expressed at high levels in the rat brain [16] has the characteristics of an adaptor protein [17,18]. FE65 which contains one WW domain and two phosphotyrosine interaction/phosphotyrosine binding domains (PID1/PID2) as an adaptor protein is able to interact with β-amyloid precursor protein (APP) through the PID2 of FE65, as well as with other members of APP protein family [19,20]. "
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    • "It also facilitates translocation of a carboxy-terminal fragment of APP to the nucleus and is required for APP-mediated transcription events. The Apbb1 protein could play an important role in the pathogenesis of AD [53]. In humans, a trinucleotide deletion in intron 13 of the Apbb1 gene was associated with a slight risk for early-onset of AD [54], while in hippocampal area CA4, increased Appb1 protein immunoreactivity seemed to be associated with the severity [55]. "
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    • "FE65 has three functional domains: two PTB domains, and one WW domain [68]. FE65 binds to NPxY sequences in members of the LDL receptor family via its PTB1 domain [31,69]. "
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