Dogs play an important role in terms of forensic science due to become reclaimable. It is
effective incident resolution that detection of dog’s biological material at the crime scene on
aggressive / victim material or detection of aggressive / victim material’s on dog’s material.
The aim of this study analysis of HV-I and HV-II regions in mtDNA from Canis lupus
familiaris blood samples and it demonstrate that it will be an important evidence source in
illumination of the incident. For this purpose, after the isolation of the DNA target regions
were amplified in the first PCR step, Exo-Sap method and ChargeSwitch ®-Pro PCR Cleanup
kit purification was performed. The second stage of PCR v3.1 Cycle Sequencing kit was used
and ABI Big Dye XTerminator and Sefadex method purification kit was performed. Index
analysis was performed in the ABI3130 capillary electrophoresis instrument. Analysis of the
raw data SeqScape 3.7 (Applied Biosystems), and the ClustalX, BioEdit programs was used.
The results obtained in 1998, Kim et al. were analyzed by comparison with published
reference number. It was established high polimorfic positions 15627, 15639, 15800, 15814,
15945, 15955, 16003, 16025 (HV-I region), 16431, 16439, 16495, 16611, 16619, 16624,
16637, 16658, 16672 (HV-II region). The results is converged of previous studies, 15 543
(4.03%) 15,640 (3.23%) 15,756 (6.45%), 15 945 (8.87), 15,951 (4.03%) , 15 960 (3.23%) in
HV-I region, 16 495 (16.94), 16 585 (7.26%), 16 611 (15.32%), 16 619 (17.74%), 16 624
(12.90% ), 16 628 (4,84%), 16 635 (8.06%), 16,637 (11.29) 16,652 (4.84%) 16,654 (4.03%)
16,658 (13.71) 16,676 (4.03%) in HV-II region. 16 haplotypes have been identified in HV-I
region; the distinction power is 0.98, the genetic diversity is 0.96. 12 haplotypes have been
identified in HV-II region, the distinction power is 0.89, genetic diversity is 0.79. As a result,
up to the number of haplotypes in HV-I region is identified and genetic diversity is high in
this area, HV-I region is according to the members of each other the higher rate of distinction
power to HV-II region.
These polymorphisms are seen for the first time. This study was performed for the first time
in Turkey, but in the field of forensic science methods used in international publications have
been optimized according to different working hours and the cost is reduced. Performed with
this arrangement is considered to contribute to the identification study.