Strategy for membrane protein crystallization exemplified with OmpA and OmpX

ArticleinProteins Structure Function and Bioinformatics 34(2):167-72 · March 1999with9 Reads
Impact Factor: 2.63 · DOI: 10.1002/(SICI)1097-0134(19990201)34:2<167::AID-PROT2>3.3.CO;2-8 · Source: PubMed

    Abstract

    The bacterial outer membrane proteins OmpA and OmpX were modified in such a manner that they yielded bulky crystals diffracting X-rays isotropically beyond 2 A resolution and permitting detailed structural analyses. The procedure involved semi-directed mutagenesis, mass production into inclusion bodies, and (re)naturation therefrom; it should be applicable for a broader range of membrane proteins.