Effect of different anticoagulant, underfilling of blood sample and storage stability on selected hemogram

ArticleinThe Kaohsiung journal of medical sciences 15(2):87-93 · March 1999with240 Reads
Source: PubMed
Abstract
We collected blood samples from 94 adult non-hematological outpatients and inpatients for complete blood count (CBC) without any flagging at Kaohsiung Medical College Hospital in order to investigate the effect of (1) different anticoagulants with Na2 EDTA vs K3 EDTA (2) the underfilling of blood collection volume (2 ml, 3. 5 ml vs standard 5 ml) (3) the difference in storage stability between 1 hour, 4 hours, 8 hours and 12 hours after venesection at room temperature on some selected hemogram parameters (WBC, RBC, hemoglobin, hematocrit, MCV, MCH, MCHC, platelet, percentage of neutrophil and lymphocyte). The automated hematology analyzer we used was SYSMEX NE-8000, (TOA, Japan). All the EDTA collection vacutainer tubes were supplied by Becton-Dickinson (New Jersey, U. S. A.) with the same lot number. Paired t- test was used for statistics. We found that values of hemoglobin, hematocrit, MCV and lymphocyte percentage collected in Na2 EDTA tubes were significantly higher than those collected in K3 EDTA (P < 0.05 for hemoglobin and lymphocyte percentage, and P all < 0.01 for others), while values of MCHC collected in Na2 EDTA were significantly lower than those collected in K3 EDTA (P < 0.05). For underfilling of blood sample, values of hematocrit and MCV with 2 ml blood volume were significantly lower than those with 5 ml blood volume (both P < 0.01), while values of MCHC with 2 ml blood volume were significantly higher than those with 5 ml blood volume (P < 0.01). When the collection blood volume was increased to 3.5 ml, there were no significant difference between values for 3.5 ml and 5ml blood volume (P all > 0.05). In the storage stability study, there was a significant sequential increase of hematocrit and MCV between 1 hour, 8 hours and 12 hours (P < 0.05 and < 0.01, respectively, for 8 hours, P all < 0.01 for 12 hours). There was also a significant sequential decrease of neutrophil percentage between 1 hour and 4, 8, 12 hours' storage at room temperature (P all < 0.01).
    • "Rapid increases in ATP turnover during intense, short-term exercise result from substrate and oxidative phosphorylation (1, 2, 3, 4, 5, 6), which causes a series of metabolic and ionic events that contribute to changes in acid base status. Ion and CO 2 movement between muscle and plasma play an essential role in that process. "
    [Show abstract] [Hide abstract] ABSTRACT: In 2003 and 2004 we examined 182 shellfish samples according to the present applicable rules. The sam- ples were tested for the presence of Salmonella and enumeration of Escherichia coli with the methods recommended by European Community. More then 90 % of samples fulfilled the veterinary conditions about placing live shellfish on the mar- ket, concerning the level of 230 E. coli in 100 g of flesh. We detected the presence of Salmonella at 0.5 % of samples. That represents a rare but possible occasional appearance of this bacteria in shellfish. Concerning other contaminants that may cause poisoning by shellfish consumption, like pathogenic strains of Vibrio parahaemolyticus, Norovirus or hepati- tis A virus, a lot of studies have been done, in a view to prepare new microbiological criteria for foodstuffs in Europe. It is now well recognised that bacterial indicators of faecal pollution (E. coli and faecal coliforms) do not adequately indicate the presence of enteric viruses. Another problem is that depuration process is more effective in removing E. coli and col- iforms than viruses from shellfish.
    Full-text · Article · Jan 2005 · Laboratory Hematology
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    [Show abstract] [Hide abstract] ABSTRACT: The erythrocyte mean cell volume (MCV) increases during in vitro storage. In aged specimens that are processed in the hematology laboratory, this phenomenon can result in misclassification of erythrocyte size. The mean cell volume is closely correlated with the mean cell hemoglobin (MCH), which does not suffer the same degree of storage-related change. These two observations offer the opportunity to perform a mathematical prediction of the MCV in aged specimens. The mathematical correction proposed in this study uses the relationship MCV = MCHC (MCH concentration)/MCH. However, instead of using a constant value for MCHC, our approach has been further refined to take account of the weak but direct relationship between MCH and MCHC. The slope and y intercept of this relationship was derived by linear regression and then used to predict an idealized MCHC, which in combination with the MCH was used to derive a predicted MCV. This method was tested in samples from 209 hospital patients using the Cell-Dyn 4000 automated hematology analyzer. The observed MCV after 24 and 48 hours of room temperature storage were on average 6.7 and 11.6 fL higher than the MCV values of the samples when processed fresh. In contrast, the mean bias of the predicted MCV values after 24 and 48 hours was -0.1 and 0.9 fL, respectively. Our study also examined the use of the Cell-Dyn 4000 white cell viability fraction (WVF) as a means of predicting when to apply the mathematical correction. The WVF of the Cell-Dyn 4000 is based on fluorescent dye exclusion by viable leukocytes, which declines during storage. A WVF threshold of 0.95 successfully separatedthe fresh samples from those stored for 24 and 48 hours. For those laboratories who process aged specimens, this offers the opportunity to report the MCV in fresh samples, while predicting and mathematically correcting the MCV in samples that are affected by age-related storage changes.
    Full-text · Article · Jul 2004
    • "Rapid increases in ATP turnover during intense, short-term exercise result from substrate and oxidative phosphorylation (1, 2, 3, 4, 5, 6), which causes a series of metabolic and ionic events that contribute to changes in acid base status. Ion and CO 2 movement between muscle and plasma play an essential role in that process. "
    Full-text · Article · · Laboratory Hematology
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