High-performance liquid chromatographic assay of (+/-)-lactic acid and its enantiomers in calf serum

ArticleinJournal of chromatography. B, Biomedical sciences and applications 727(1-2):23-9 · April 1999with16 Reads
DOI: 10.1016/S0378-4347(99)00072-9 · Source: PubMed

    Abstract

    Two high-performance liquid chromatographic methods are described for the determination of lactic acid and its enantiomers in calf serum. A 300x8.0 mm I.D. column packed with sulfonated styrene-divinylbenzene copolymer and a 50x4.6 mm ODS column with N,N-dioctyl-L-alanine were used. UV detection was at 205 and 236 nm for the non-chiral and chiral assays, respectively. Both assays demonstrated excellent linear relationships between peak area ratios and serum concentrations over a range of 0.5 to 20 mM, based on 100 microl bovine serum. Recovery was complete. Inter- and within-batch bias and relative standard deviation were <15%.