Secretory leukocyte protease inhibitor (SLPI) concentrations in cervical mucus of women with normal menstrual cycle

Department of Obstetrics and Gynecology, Faculty of Medicine, Osaka University, 2-2 Yamada-oka, Suita City, Osaka 565-0871, Japan.
Molecular Human Reproduction (Impact Factor: 3.75). 08/1999; 5(7):656-61. DOI: 10.1093/molehr/5.7.656
Source: PubMed


Secretory leukocyte protease inhibitor (SLPI) is a potent inhibitor of human leukocyte elastase. SLPI transcripts in the cervical tissue were detected during the menstrual cycle by reverse transcription-polymerase chain reaction (RT-PCR). Western blot analysis revealed that the intensity of SLPI protein in cervical tissue in the ovulatory phase was stronger than in other phases. Immunohistochemistry using an anti-SLPI polyclonal antibody revealed positive staining in the epithelial cells of the endocervix. Western blot analysis also revealed that SLPI protein was present in the cervical mucus. Again the intensity of SLPI protein in the ovulatory phase was stronger than that in the follicular phase. The SLPI concentrations and SLPI/elastase ratios in the cervical mucus of women in the ovulatory phase were significantly higher than in the follicular and luteal phases. The SLPI and elastase concentrations in the cervical mucus were positively correlated. No significant difference was found in the SLPI serum concentrations of women during the menstrual cycle. These results suggest that production of SLPI from cervical epithelial cells during the ovulatory phase may be important for protection from the effects of elastase.

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Available from: Tadashi Kimura
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    • "Human epithelial and myeloid cells constitutively secrete SLPI, and cellular production can be significantly upregulated in response to various stimuli [69]. Concentrations in cervical mucosal fluids are commonly reported at 1 µg/ml [70], but are likely to be more concentrated at the cell surface. In addition, SLPI levels in cervical mucus have been measured midcycle as high as 78 µg/mL [71]. "
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