In vitro isolation of Neospora caninum from a stillborn calf in the UK

ArticleinResearch in Veterinary Science 67(1):103-5 · September 1999
Impact Factor: 1.41 · DOI: 10.1053/rvsc.1998.0272 · Source: PubMed


    Neospora caninum was isolated in Vero cell culture from the brain of a stillborn calf. This isolate (designated NC-LivB1) is the first to be obtained from cattle in the United Kingdom and was confirmed as N. caninum by immunofluorescence with specific antibodies and by internal transcribed spacer 1 (ITS1) sequence analysis. Differences were found between NC-LivB1, other bovine isolates and canine isolates of N. caninum and closely related protozoal parasites, using random amplified polymorphic DNA polymerase chain reaction (RAPD - PCR) techniques.