Simopoulos AP (ed): Evolutionary Aspects of Nutrition and Health.
Diet, Exercise, Genetics and Chronic Disease.
World Rev Nutr Diet. Basel, Karger, 1999, vol 84, pp 19–73
Humanity’s Double-Edged Sword
Department of Exercise and Sport Science, Colorado State University, Fort Collins,
‘Here is bread, which strengthens man’s heart, and therefore called the staﬀof life’
(Mathew Henry: 1662–1714, Commentary on Psalm 104)
‘Man cannot live on bread alone’ (Bible, Matthew 4:4)
22 Archaeological Perspective
24 Dietary Imbalances of Cereal Grains
26 Vitamins A, C and Beta-Carotene
27 B Vitamins
34 Essential Fatty Acids
36 Amino Acids
41Antinutrients in Cereal Grains
43 Alpha-Amylase Inhibitors
44 Protease Inhibitors
47 Autoimmune Diseases and Cereal Grain Consumption
49 Molecular Mimicry
49 Genetic and Anthropological Factors
51Autoimmune Diseases Associated with Cereal Grain Consumption
56 Psychological and Neurological Illnesses Associated with Cereal Grain Consumption
The number of plant species which nourish humanity is remarkably lim-
ited. Most of the 195,000 species of ﬂowering plants produce edible parts
which could be utilized by man; however less than 0.1% or fewer than 300
species are used for food. Approximately 17 plant species provide 90% of
mankind’s food supply, of which cereal grains supply far and away the greatest
percentage (tables 1, 2). From table 1, it can be shown that the world’s four
major cereal grains (wheat, maize, rice and barley) contribute more tonnage
Table 1 . The world’s top 30 food crops
(estimated edible dry matter) Million metric tons
1 Wheat 468
2 Maize 429
3 Rice 330
4 Barley 160
5 Soybean 88
6 Cane sugar 67
7 Sorghum 60
8 Potato 54
10 Cassava 41
11 Sweet potato 35
12 Beet sugar 34
13 Rye 29
14 Millets 26
15 Rapeseed 19
16 Bean 14
17 Peanut 13
18 Pea 12
19 Musa 11
20 Grape 11
21 Sunﬂower 9.7
22 Yams 6.3
23 Apple 5.5
24 Coconut 5.3
25 Cottonseed (oil) 4.8
26 Orange 4.4
27 Tomato 3.3
28 Cabbage 3.0
29 Onion 2.6
30 Mango 1.8
Adapted from Harlan .
to humanity’s food supply than the next 26 crops combined. Eight cereal
grains: wheat, maize, rice, barley, sorghum, oats, rye, and millet provide 56%
of the food energy and 50% of the protein consumed on earth . Three
cereals: wheat, maize and rice together comprise at least 75% of the world’s
grain production (table 1). It is clear that humanity has become dependent
upon cereal grains for the majority of its food supply. As Mangelsdorf  has
pointed out, ‘cereal grains literally stand between mankind and starvation’;
therefore, it is essential that we fully understand the nutritional implications
of cereal grain consumption upon human health and well being.
Modern man has become so dependent upon eating cereal grains (grass
seeds) that it has prompted at least one author  to say that we have become
‘canaries’. However, this has not always been the case. For the vast majority
of mankind’s presence on this planet, he rarely if ever consumed cereal grains
. With the exception of the last 10,000 years following the agricultural
‘revolution’, humans have existed as non-cereal-eating hunter-gatherers since
the emergence of Homo erectus 1.7 million years ago. Although the ﬁrst
anatomically modern humans (Homo sapiens) appeared in Africa ?90,000
years ago, humans prior to the mesolithic period (~15,000 years ago) like
other primates rarely if ever utilized cereal grains . Post-pleistocene (~10,000
years ago) hunter-gatherers occasionally consumed cereal grains; however
these foods were apparently not major dietary components for most of the
year . It is apparent that there is little or no evolutionary precedent in our
species for grass seed consumption [6–8]. Consequently, we have had little
time (=500 generations) since the inception of the agricultural revolution
10,000 years ago to adapt to a food type which now represents humanity’s
major source of both calories and protein.
The sum of evidence indicates that the human genetic constitution has
changed little in the past 40,000 years . The foods which were commonly
Table 2 . Food group totals (estimated edible dry matter)
Million metric tons
1 Cereals 1,545
2 Tubers 136
3 Pulses 127
4 All meats, milk and eggs 119
5 Sugar 101
6 Fruits 34
Adapted from Harlan .
21Cereal Grains: Humanity’s Double-Edged Sword
Table 3. Key events in the development of agriculture and domestication of cereal grains
Event Time from present Location
Development of agriculture 10,000 Near East
8,000 Greece, West Africa
7–8,000 Central and S. America
7,000 China, India and SE Asia
6,500 Paris basin
6,000 Central Africa
5,500 Scandinavia, England
Domestication of wheat and barley 10,000 Near East
Domestication of rice 7,000 China, India and SE Asia
Domestication of maize 7,000 Central and S. America
Domestication of millets 5–6,000 Africa
Domestication of sorghum 5–6,000 East Africa
Domestication of rye 5,000 SW Asia
Domestication of oats 3,000 Europe
available to preagricultural man were the foods which shaped modern man’s
genetic nutritional requirements. Although our genetically determined nutri-
tional needs have changed little in the past 40,000 years, our diet has changed
dramatically since the advent of agriculture 10,000 years ago . Cereal grains
as a staple food are a relatively recent addition to the human diet (table 3) and
represent a dramatic departure from those foods to which we are genetically
adapted. Discordance between humanity’s genetically determined dietary
needs and his present day diet is responsible for many of the degenerative
diseases which plague industrial man . Although cereal grains are associated
with virtually every highly developed civilization in mankind’s history and
now occupy the base of the present day food selection pyramid in the United
States , there is a signiﬁcant body of evidence which suggests that cereal
grains are less than optimal foods for humans and that the human genetic
makeup and physiology may not be fully adapted to high levels of cereal grain
At the close of the paleolithic era and during the mesolithic period (20,000–
10,000 years ago), there was a widescale extinction of large mammals through-
out Europe, North America and Asia  that coincided with a fundamental
change in how hunter-gatherer’s made use of their environment and obtained
their food sources. People all over the world began to adopt a broader spectrum
of hunting and gathering which more fully utilized all niches in their environ-
ment. Tools and weapons became smaller, more elegant and more eﬃcient
. The aquatic environment was increasingly exploited via boats, canoes,
harpoons, ﬁsh nets, hooks and weirs. Birds and waterfowl began to appear
more frequently in the fossil record associated with man’s food supply. For
the ﬁrst time (15,000 years ago) grindstones and crude mortars appeared in
the archaeological record in the near east , thereby heralding the beginnings
of humanity’s use of cereal grains for food. Since wild cereal grains are small,
diﬃcult to harvest and minimally digestible without processing (grinding) and
cooking [5, 12, 13], the appearance of stone-processing tools is an essential
indication of when and where cultures began to include cereal grains in their
As human population numbers increased following the pleistocene (10,000
years ago) and as large grazing herbivores became either extinct or severely
depleted, humanity became more and more reliant upon small mammals, ﬁsh,
fowl and gathered plant foods to supply his caloric needs. Gradually, as these
resources became depleted, in the face of increasing human population num-
bers, agriculture became the dominant way of life, and cereal grains became
the dominant caloric and protein source in many, but not all prehistoric
cultures [3, 14]. Whereas hunter-gatherers derived most of their calories from
a diversity of wild animal meats, fruits and vegetables encompassing between
100 and 200 or more species , agricultural man became primarily dependent
upon a few staple cereal foods, 3–5 domesticated meats and between 20 and
50 other plant foods. In many third-world countries and in a number of
historical agrarian societies, a single cereal staple could provide up to 80% or
more of the daily caloric intake with few or no calories regularly coming from
animal sources [7, 16].
Generally, in most parts of the world, whenever cereal-based diets were
ﬁrst adopted as a staple food replacing the primarily animal-based diets of
hunter-gatherers, there was a characteristic reduction in stature [4, 17–19], an
increase in infant mortality [19, 20], a reduction in lifespan [19, 20], an increased
incidence of infectious diseases [19–22], an increase in iron deﬁciency anemia
[19, 20, 22], an increased incidence of osteomalacia, porotic hyperostosis and
other bone mineral disorders [4, 19, 20, 22] and an increase in the number of
dental caries and enamel defects [19, 20, 23]. In a review of 51 references
examining human populations from around the earth and from diﬀering chro-
nologies, as they made the transition from hunter-gatherers to farmers, Cohen
 concluded that there was anoverall decline in both the qualityand quantity
of life. There is now substantial empirical and clinical evidence to indicate that
23Cereal Grains: Humanity’s Double-Edged Sword
many of these deleterious changes may be directly related to the predominantly
cereal-based diet of these early farmers.
Cereal grains truly represent humanity’s double-edged sword, for without
them we likely would not have had an agricultural ‘revolution’. We surely
would not be able to sustain the enormous present-day human population
(?6 billion), nor would there likely have been societal stratiﬁcation which
ultimately was responsible for the vast technological/industrial culture in which
we live . The enormous increase in human knowledge would probably never
had taken place had it not been for the widespread adoption of agriculture by
humanity, and our understanding of medicine, science and the universe is
a direct outcome of the societal stratiﬁcation wrought by the agricultural
‘revolution’ . On the other hand, agriculture is generally agreed to be
responsible for many of humanity’s societal ills including whole-scale warfare,
starvation, tyranny, epidemic diseases, and class divisions . Cereals provide
the major caloric and protein source for humanity and therefore are the
mainstay of agriculture; they have allowed man’s culture to grow and evolve
so that man has become earth’s dominant animal species, but this preeminence
has not occurred without cost. Because of cereal grains mankind has dramatic-
ally altered his original culture; moreover cereal grains have fundamentally
altered the foods to which our species had been originally adapted over eons
of evolutionary experience. For better or for worse, we are no longer hunter-
gatherers, however our genetic makeup is still that of a paleolithic hunter-
gatherer, a species whose nutritional requirements are optimally adapted to
wild meats, fruits and vegetables, not to cereal grains. We have wandered down
a path toward absolute dependence upon cereal grains, a path for which there
is no return. It is critical that we fully understand the nutritional shortcomings
of cereal grains as we proceed.
Dietary Imbalances of Cereal Grains
All cereal grains have signiﬁcant nutritional shortcomings which are
apparent upon analysis. From table 4 it can be seen that cereal grains contain
no vitamin A and except for yellow maize, no cereals contain its metabolic
precursor, beta-carotene. Additionally, they contain no vitamin C, or vitamin
. In most western, industrialized countries, these vitamin shortcomings
are generally of little or no consequence, since the average diet is not
excessively dependent upon grains and usually is varied and contains meat
(a good source of vitamin B
), dairy products (a source of vitamins B
and A), and fresh fruits and vegetables (a good source of vitamin C and
Table 4. Vitamin and mineral content of eight unprocessed cereal grains (100-gram
Wheat Maize Rice Barley Sorghum Oats Rye Millet
, mg 0.38 0.39 0.40 0.65 0.24 0.76 0.32 0.42
(35%) (35%) (36%) (59%) (22%) (69%) (29%) (38%)
, mg 0.12 0.20 0.09 0.29 0.14 0.14 0.25 0.29
(9%) (15%) (7%) (22%) (11%) (11%) (19%) (22%)
, mg 5.47 3.63 5.09 4.60 2.92 0.96 4.27 4.72
(36%) (24%) (34%) (31%) (20%) (6%) (28%) (31%)
, mg 0.30 0.62 0.51 0.32 n.a. 0.12 0.29 0.38
(21%) (39%) (32%) (20%) (n.a.) (7%) (18%) (24%)
Folate, mg 38.2 19.0 19.5 19.0 n.a. 56.0 59.9 85.0
(21%) (11%) (11%) (11%) (n.a.) (31%) (33%) (47%)
Pantothenic acid, mg 0.95 0.42 1.49 0.28 n.a. 1.35 1.46 0.85
(17%) (8%) (27%) (5%) (n.a.) (24%) (26%) (15%)
Biotin n.a. n.a. n.a. n.a. n.a. n.a. n.a. n.a.
(n.a.) (n.a.) (n.a.) (n.a.) (n.a.) (n.a.) (n.a.) (n.a.)
E, mg n.a. 0.49 0.68 0.57 n.a. 1.09 1.28 0.05
(n.a.) (6%) (9%) (7%) (n.a.) (14%) (16%) (1%)
Potassium, mg 363 287 223 452 350 429 264 195
(18%) (14%) (11%) (23%) (17%) (21%) (13%) (10%)
Sodium, mg 2 35 7 12 6 2 6 5
(0%) (1%) (0%) (1%) (0%) (0%) (0%) (0%)
Calcium, mg 29.0 7.0 23.0 33.0 28.0 53.9 33.0 8.0
(4%) (1%) (3%) (4%) (4%) (7%) (4%) (1%)
Phosphorus, mg 288 210 333 264 287 523 374 285
(36%) (26%) (42%) (33%) (36%) (65%) (47%) (36%)
Magnesium, mg 126 127 143 133 n.a. 177 121 114
(45%) (45%) (51%) (48%) (n.a.) (63%) (43%) (41%)
Iron, mg 3.19 2.71 1.47 3.60 4.40 4.72 2.67 3.01
(21%) (18%) (10%) (24%) (29%) (31%) (18%) (20%)
Zinc, mg 2.65 2.21 2.02 2.77 n.a. 3.97 3.73 1.68
(22%) (18%) (17%) (23%) (n.a.) (33%) (31%) (14%)
Copper, mg 0.43 0.31 0.27 0.50 n.a. 0.63 0.45 0.75
(19%) (14%) (12%) (22%) (n.a.) (28%) (20%) (33%)
Manganese, mg 3.98 0.46 3.75 1.95 n.a. 4.92 2.68 1.63
(114%) (14%) (107%) (56%) (n.a.) (140%) (77%) (47%)
Selenium, mg 0.043 0.004 n.a. 0.066 n.a. n.a. n.a. n.a.
(78%) (8%) (n.a.) (120%) (n.a.) (n.a.) (n.a.) (n.a.)
Values in (parentheses) represent RDA %. n.a.>Not available. No detectable amounts
of vitamins A, C, D, B
in any grain.
25Cereal Grains: Humanity’s Double-Edged Sword
However, as more and more cereal grains are included in the diet, they
tend to displace the calories that would be provided by other foods (meats,
dairy products, fruits and vegetables), and can consequently disrupt adequate
nutritional balance. In some countries of Southern Asia, Central America,
the Far East and Africa cereal product consumption can comprise as much
as 80% of the total caloric intake , and in at least half of the countries of
the world, bread provides more than 50% of the total caloric intake . In
countries where cereal grains comprise the bulk of the dietary intake, vitamin,
mineral and nutritional deﬁciencies are commonplace.
Vitamins A, C and Beta-Carotene
Vitamin A deﬁciency remains one of the major public health nutritional
problems in the third world . Twenty to 40 million children worldwide are
estimated to have at least mild vitamin A deﬁciency . Vitamin A deﬁciency
is a leading cause of xerophthalmia and blindness among children and also
a major determinant of childhood morbidity and mortality . In virtually
all infectious diseases, vitamin A deﬁciency is known to result in greater
frequency, severity, or mortality . A recent meta-analysis  from 20
randomized controlled trials of vitamin A supplementation in third world
children has shown a 30–38% reduction in all cause mortality in vitamin A-
supplemented children. Analysis of cause-speciﬁc mortality showed vitamin
A supplementation elicited a reduction in deaths from diarrheal disease by
39%, from respiratory disease by 70% and from all other causes of death by
34% . Clearly, the displacement of beta-carotene-containing fruits and
vegetables and vitamin A-containing foods (milk fat, egg yolks and organ
meats) by excessive consumption of cereal grains plays a major role in the
etiology of vitamin A deﬁciency in third world children.
In numerous epidemiologic studies, an increased intake of fruits and
vegetables has been associated with a reduced risk of many types of cancer
[29, 30] and coronary heart disease (CHD) [31, 32]. Much of the evidence for
the link between fruit, vegetables and cancer and CHD points to those foods
rich in antioxidants, including vitamin C, carotenoids and phytochemicals. In
the United States, an estimated 45% of the population had no servings of
fruit or juice, and 22% had no servings of a vegetable on any given day
. Further, 91% of the adult population did not meet the United States
Department of Agriculture’s daily recommendation of 2–3 servings of fruit
and 3–5 servings of vegetables . Although frank vitamin C deﬁciency is
virtually unknown in the United States and other western countries, it has
been shown to be common in portions of rural India wherein cereals and
pulses comprise the dietary mainstays, and vitamin C-rich fruits and vegetables
are consumed in low quantities . Again, since cereal grains contain unde-
tectable amounts of vitamin C and carotenoids, they tend to displace foods
rich in these substances; foods which are associated with a decreased risk for
many common cancers  and heart disease [31, 32].
Cereal- and pulse-based diets of the third world generally tend to be
considerably lower in both total fat, saturated fat and cholesterol than the
meat-based diets of western countries , yet paradoxically, CHD mortality
is in some cases either higher  or similar [36, 37] to that in western countries.
Since the antioxidant status of CHD-prone individuals chronically consuming
cereal- and pulse-based diets has been shown to be low [36, 38], and increased
consumption of fruit and vegetables has been shown to improve the CHD
risk proﬁle of this population , it is likely that high cereal grain consumption
partially contributes to increased CHD mortality via its displacement of anti-
oxidant rich fruits and vegetables.
Diets based primarily or wholly upon plant food sources tend to be either
low or deﬁcient in vitamin B
, since this nutrient is found exclusively in animal
products . Vitamin B
deﬁciency causes a megaloblastic anemia which
ultimately results in cognitive dysfunction via its irreversible impact on the
neurological system . Additionally, it is known that a chronic B
produces elevated homocysteine levels [42, 43] which are an important risk
factor for arterial vascular disease and thrombosis [43, 44]. Vitamin B
ciency is generally assumed to be uncommon because omnivorous diets provide
adequate intake, and the vitamin is conserved eﬃciently by the enterohepatic
circulation . However, in countries such as India in which the diets are
mainly cereal and pulse based, vitamin B
deﬁciencies are common [45, 46].
Additionally, even if minimal amounts of animal-based foods are consumed
along with traditional cereal- and pulse-based diets, intestinal infection, which
is widespread in the third world, has been shown to worsen an already compro-
status and result in widespread B
deﬁciencies . The human
nutritional requirement for vitamin B
clearly demonstrates that vegetarian
diets based entirely upon cereal grains, legumes and other plant foods were
not the sole dietary components which shaped the human genome.
Many nutritionists consider cereal grains to be good sources of most of
the B vitamins except for vitamin B
. Inspection of table 4 generally is support-
ive of this concept, at least in terms of the % RDA which cereal grains contain.
However, of more importance is the biological availability of the B vitamins
contained within cereal grains and their B vitamin content after milling,
processing and cooking. It is somewhat ironic that two of the major B vitamin
deﬁciency diseases which have plagued agricultural man (pellagra and beriberi)
are almost exclusively associated with excessive consumption of cereal grains.
27Cereal Grains: Humanity’s Double-Edged Sword
Beriberi occurs from a thiamin deﬁciency which is associated with polished
rice consumption. In the late 1800s, with the introduction of polished rice,
beriberi reached epidemic proportions in Japan and other countries in South-
east Asia . Human crossover experiments done in the early part of this
century induced beriberi in subjects fed polished rice, but not in those fed
brown rice . The removal of the outer thiamin-containing coat of the rice
kernel during the polishing process was found to be the factor responsible for
inducing beriberi in rice-eating populations . Beriberi has been largely
eliminated with the advent of ‘enriched rice’ to which thiamin is added, but
still occurs in some African countries whose populations consume high quanti-
ties of polished rice .
Pellagra is thought to be a multiple deﬁciency disease caused by a lack of
niacin and the essential amino acid tryptophan , and occurs almost exclus-
ively in people eating corn as their staple food. In the United States between
1906 and 1940 there was an epidemic of pellagra in the southern states which
resultedinapproximately3 million caseswith at least 100,000 deaths.Similar
epidemicshave occurred in Europe and India , and pellagraisstillwidespread
in parts of Africa [52, 53]. Although administration of niacin is known to rapidly
eliminate all symptoms of pellagra, there is a continuing suspicion that not all
of the precipitating factors which operate in maize to elicit overt symptoms of
pellagra are understood [54, 55]. Traditional lime-processing techniques of corn
(boiling of dried corn ﬂour for 30–50 min in a 5% lime water solution) prevents
pellagra, and it is thought to do so by increasing niacin’s availability . How-
ever, a modern study  recently reanalyzed historical pellagra-inducing diets
and even after correcting for niacin’s low availability, found these diets to be
adequate in niacin equivalents (niacin+0.0166¶tryptophan), suggesting that
factors in corn other than low niacin and tryptophan were responsible for the
are known to decrease intestinal absorption of many key nutrients [56, 57]. Since
villous atrophy of the small intestine has been demonstrated in patients with
pellagra , it is possible that certain antinutrients in maize could interfere
with intestinal absorption of both niacin and tryptophan or that plasma-borne
antinutrients could interfere with the conversion of tryptophan to niacin similar
to the eﬀects of isoniazid, an anti-tuberculous drug which is known to produce
pellagra-like symptoms .
Although table 4 suggests that most cereal grains except for oats are
relatively good sources of vitamin B
, the bioavailability of B
grains tends to be low, whereas bioavailability of B
from animal products is
generally quite high, approaching 100% . Vitamin B
exists in foods as
three nonphosphorylated forms (pyridoxine, pyridoxal and pyridoxamine) and
two phosphorylated forms of pyridoxal and pyridoxamine. An additional
glycosylated adduct of pyridoxine, pyridoxine glucoside, occurs widely in cereal
grains and has been shown to reduce the bioavailability of both nonphos-
phorylated and phosphorylated forms of vitamin B
by 75–80% [60, 61]. The
presence of pyridoxine glucoside in cereal grains has an overall eﬀect of
depressing the vitamin B
nutritional status . Data from Nepalese vege-
tarian lactating women has shown a low vitamin B
status for both the mothers
and their infants which was partially attributed to the high levels of pyridoxine
glucosides found in their cereal-, legume- and plant-based diet . B
ciencies appear to be quite common in populations utilizing cereals and pulses
as staples [63, 64]. Low tissue levels of vitamin B
, like vitamin B
to elevate plasma homocysteine levels and increase the risk for arterial vascular
disease . To date, plasma homocysteine levels have not been evaluated in
cereal- and pulse-eating populations of the Indian subcontinent wherein there
is a high mortality rate from CHD .
Perhaps the least studied of the B complex vitamins is biotin. Animal
studies have shown that most cereal grains except maize have very low levels
of bioavailable biotin [65, 66], whereas foods derived from animal sources
have a high biotin digestibility . Both wheat and sorghum not only have
a low biotin bioavailability, but seem to have elements within them which
seem to elicit a depression of biotin metabolism . The enzyme, biotinidase,
recycles the biotin derived from the turnover of the biotin-dependent car-
boxylases and from exogenous protein-bound dietary biotin (ﬁg. 1). Whether
or not antinutrients present in cereal grains interfere with biotinidase is not
known. However, the biotin-dependent carboxylases are important metabolic
pathways of fatty acid synthesis. A biotin deﬁciency severely inhibits the chain
elongation and desaturation of linoleic acid to arachidonic acid  (ﬁg. 2),
and biotin-deﬁcient rats are known to exhibit prominent cutaneous symptoms
including scaling, seborrheic dermatitis and alopecia , symptoms which
are identical in humans with biotin and biotinidase deﬁciencies. Recent human
biotin supplementation trials have shown this vitamin to reduce ﬁngernail
brittleness . Anecdotal evidence has suggested that subjects who had
adopted the Pritikin diet (a low-fat diet based primarily upon cereal grains)
for periods of 1–2 years developed vertical ridges on their ﬁngernails . It
is unclear if these symptoms are caused by impaired biotin metabolism; how-
ever the available research on this poorly studied vitamin suggests that diets
based primarily upon cereal grains are responsible for causing biotin deﬁcien-
cies in a variety of laboratory animals.
Table 4 displays the mineral content and the percent of the recommended
daily allowance (RDA) in a 100-gram sample of the world’s most commonly
29Cereal Grains: Humanity’s Double-Edged Sword
Fig. 1. Biotin metabolism. Biotin-dependent carboxylation reactions can be divided
into step 1 (the formation of carboxyl biotinyl enzyme), and step 2 (carboxyl transfer to an
appropriate acceptor substrate, dependent upon the speciﬁc transcarboxylase involved).
consumed cereal grains. Of the minerals, cereal grains are poor sources of
sodium and calcium but are relatively rich sources of phosphorous, potassium
and magnesium. Not all of the minerals are included in table 4; however it
can be seen that cereal grains contain moderate amounts (10–33%) of zinc,
copper and iron and high amounts of manganese.
Calcium. Except for calcium and sodium, it would appear that cereal
grains provide reasonable amounts of most minerals needed for adequate
nutrition. Since the western diet is already overburdened by high dietary
sodium levels , the low sodium content of cereal grains is desirable. In
most western populations that consume a mixed diet, the low calcium content
of cereal grains does not normally represent a problem since dairy products
and leafy green vegetables are good sources of calcium, if they are included
in the diet. However, as is the case for vitamins, as more and more cereal
grains are included in the diet, they tend to displace dairy and vegetable
sources of calcium. Further, cereal grains have a Ca/P ratio which is quite
low (mean from table 4>0.08) and which can negatively impact bone growth
and metabolism. Consumption of a large excess of dietary phosphorus, when
calcium intake is adequate or low, leads to secondary hyperparathyroidism
and progressive bone loss . The recommended, ideal Ca/P ratio is 1:1,
Fig. 2. The essential fatty acids and their long-chain polyunsaturated metabolites.
whereas in the United States it averages 0.64 for women and 0.62 for men
. In addition to the unfavorable Ca/P ratio, cereal grains maintain a quite
low Ca/Mg ratio (averaging 0.19 from table 4) which also favors net Ca
excretion, since imbalances in Mg intake relative to Ca decrease gastrointesti-
nal absorption and retention of Ca [73, 74]. Because of the high phytate
content of whole grain cereals much of the calcium present is unavailable
for absorption because the phytate forms insoluble complexes with calcium
. The net eﬀect of a low calcium content, a low Ca/P ratio, a low Ca/
Mg ratio, and low bioavailability of calcium via a high phytate content
frequently induces bone mineral pathologies in populations dependent upon
cereal grains as a staple food. In populations where cereal grains provide
the major source of calories, osteomalacia, rickets and osteoporosis are com-
monplace [76–79]. Cereal grains have been shown to cause their rachitogenic-
and osteomalacia-producing eﬀects in spite of the presence of adequate sun-
shine . Further, substitution of leavened white breads of lower extraction
for unleavened whole grain breads improved biochemical symptoms in pa-
tients with rickets or osteomalacia .
31Cereal Grains: Humanity’s Double-Edged Sword
Consumption of high levels of whole grain cereal products impairs bone
metabolism not only by limiting calcium intake, but by indirectly altering
vitamin D metabolism. In animal studies it has been long recognized that
excessive consumption of cereal grains can induce vitamin D deﬁciencies in
a wide variety of animals [81–83] including primates . Epidemiological
studies of populations consuming high levels of unleavened whole grain breads
show vitamin D deﬁciency to be widespread [85–87]. A study of radiolabelled
) in humans consuming 60 g of wheat bran
daily for 30 days clearly demonstrated an enhanced elimination of 25(OH)D
in the intestinal lumen . The mechanism by which cereal grain consumption
inﬂuences vitamin D is unclear. Some investigators have suggested that cereal
grains may interfere with the enterohepatic circulation of vitamin D or its
metabolites [84, 88], whereas others have shown that calcium deﬁciency in-
creases the rate of inactivation of vitamin D in the liver . This eﬀect is
mediated by 1,25-dihydroxyvitamin D (1,25(OH)
D) produced in response to
secondary hyperparathyroidism, which promoteshepatic conversion of vitamin
D to polar inactivation products which are excreted in bile . Consequently,
the low Ca/P ratio of cereal grains has the ability to elevate PTH which in
turn stimulates increased production of 1,25(OH)
D which causes an acceler-
ated loss of 25-hydroxyvitamin D.
Iron. In addition to their deleterious inﬂuence upon calcium metabolism,
cereal grains when consumed in excessive quantities can adversely inﬂuence
iron metabolism. Because of their ﬁber and phytate content, the bioavailability
of iron in cereal grains is quite low [75, 90]. Iron deﬁciency is the most prevalent
nutritional problem in the world today aﬀecting 2.15 billion people throughout
the world and being severe enough to cause anemia in 1.2 billion people
[91, 92]. The causative factor has been clearly demonstrated to be the poor
bioavailability of iron from cereal-based diets, which are the staple food in
many developing countries . The displacement of iron-rich animal foods
by cereal grains, legumes and plant-based diets is thus largely indirectly respon-
sible for the worldwide epidemic of iron deﬁciency. Iron deﬁciency is known
to reduce work capacity and productivity in adults, increase the severity and
incidence of infection, and increase maternal, prenatal and perinatal mortality
. Perhaps the most serious eﬀect of iron deﬁciency is the often irreversible
impairment of a child’s learning ability .
There appear to be a number of elements within cereal grains which may
inhibit nonheme iron absorption including phytate , tannins , ﬁber ,
lectins , phosphate  and perhaps other unknown factors . However,
the primary inhibitor of nonheme iron absorption by cereal grains is its phytate
content . Recent work has indicated that phytate must be almost totally
removed to eliminate its inhibitory eﬀect on nonheme iron absorption .
Consequently, diets based upon whole grain maize , rice , wheat
 and oats  have been consistently shown to reduce iron absorption.
Nonheme iron absorption can be enhanced by including ascorbate-rich fruit
and vegetables with cereal-based meals . Further, the addition of yeast
fermentation to make leavened breads is known to reduce their phytate content
. Additionally, fortiﬁcation of cereal grains with iron has been shown to
be an eﬀective procedure to prevent iron deﬁciency anemia [104, 105].
Other Minerals. In addition to calcium and iron, the bioavailability of
zinc, copper and magnesium in cereal grains is generally low , whereas the
absorption of manganese, chromium and selenium does not appear to be
impaired . Except for zinc, the clinical implications of deﬁciencies in these
minerals relative to cereal grain consumption have been poorly studied. Con-
sequently, few links have been established between high cereal grain consump-
tion and deﬁciencies of copper, magnesium, manganese, chromium and
selenium in human diets. However, there is substantial evidence which demon-
strates that relatively high consumption of cereal grains can have a detrimental
inﬂuence upon zinc metabolism and thus adversely aﬀect human health and
Zinc. Radiolabelled studies of zinc absorption in rats  and humans
 have clearly demonstrated that consumption of whole grain cereals
(wheat, rye, barley, oats and triticale) impairs zinc absorption. Similar to iron,
it appears that phytate plays a major role in the inhibition of zinc absorption
[106, 107]; however, other factors are likely involved . In humans, zinc
deﬁciency results in a characteristic syndrome called hypogonadal dwarﬁsm
in which there is arrested growth, hypogonadism and delayed onset of puberty
. In rural Iran where unleavened, whole grain ﬂat bread (tanok) contrib-
utes at least 50% of the daily calories , the incidence of hypogonadal
dwarﬁsm was estimated to be nearly 3% in 19-year-old conscripts . Since
the zinc intake of these populations exceeds the RDA by a substantial margin
, it has been shown that the high consumption of tanok is responsible
for inducing negative zinc balances . Recent studies of nonhuman primates
moderately deprived of zinc  as well as zinc supplementation trials in
children  have conﬁrmed Reinhold’s earlier work  showing how
marginal zinc nutriture, independent of other nutrients, may limit skeletal
growth. Yeast leavening of whole grain breads can reduce their phytate content
and improve the bioavailability of zinc ; however increased ascorbic acid
intake does not enhance the absorption of zinc . Because the bioavailability
of zinc from meat is four times greater than that from cereals , it is clear
that the displacement of animal-based foods by cereal-grain- and plant-based
diets is not only responsible for impaired zinc metabolism in developing coun-
tries, but also in western populations adopting vegetarian diets [114, 115].
33Cereal Grains: Humanity’s Double-Edged Sword
Essential Fatty Acids
Cereal grains are quite low in fats (table 6) averaging 3.6% fat for their
total caloric content; even still a predominantly cereal- and plant-based diet
can contribute 5–10 g per person per day of linoleic acid (LA), the major X-
6 (n-6) polyunsatuarated fatty acid found in grains . The linolenic acid
content of cereals is quite low, and they are devoid of the longer chain X-3
(n-3) derivatives of linolenic acid, including eicosapentaenoic acid (EPA) and
docosahexaenoic acid (DHA). Consequently, cereal-based diets, particularly
if they are supplemented by vegetable oils, tend to have a high n-6/n-3 ratio
(table 6) and are deﬁcient in EPA, DHA and long-chain derivatives of LA
including arachidonic acid (AA).
In man, the longer chain fatty acids can be synthesized from their shorter
chain precursors; however the process is ineﬃcient , and because linoleic
and linolenic acid must utilize the same desaturase and elongase enzymes,
there is competitive inhibition of one another, so that high dietary levels of
linoleic acid tends to inhibit the formation of EPA from linolenic acid if
preformed EPA is not obtained directly in the diet from ﬁsh or meat sources.
The importance of certain long-chain fatty acids [20:3n-6 (dihomogammalin-
olenic acid), 20:4n-6 (AA) and 20:5n-3 (EPA)] is that they serve as precursors
for the synthesis of eicosanoids (the prostaglandins, prostacyclins, thrombox-
anes, and leukotrienes), potent hormone-like substances which have a variety
of eﬀects including regulation of platelet aggregation, thrombosis and in-
ﬂammation . Increased dietary consumption of n-3 fatty acids, particu-
larly EPA has been shown to decrease triglycerides, decrease thrombotic
tendencies  and reduce symptoms of many inﬂammatory and auto-
immune diseases including arthritis  and inﬂammatory bowel disease
. Additionally, epidemiological studies indicate a reduced mortality from
coronary heart disease in populations consuming increased amounts of n-3
fatty acids .
Vegetarian diets based primarily upon cereals, legumes and plant products
are known to have a high n-6/n-3 ratio because of their low levels of both
linolenic acid and the absence of its long-chain derivatives, EPA and DHA
. Studies of preterm infants deprived of DHA have shown both visual
and cortical abnormalities . A recent study of South Asian vegetarian
mothers has indicated lower plasma levels of EPA and DHA when compared
to white nonvegetarians . Additionally, cord DHA levels were lower in
the vegetarian mothers, and the duration of gestation was 5.6 days shorter
than the meat-eating controls. In the vegetarian women early onset of labor
and emergency cesarean section were more common, and birth weight, head
circumference and body length were lower in the infants born to the vegetarian
In the United States, the US Department of Agriculture has recently
adopted a ‘food pyramid’ of nutritional recommendations that places grains
and pasta at the bottom (i.e. to be eaten in the largest amounts; 6–11 servings
per day). It has recently been argued that a diet of this nature likely encourages
essential fatty acid (EFA) deﬁciencies and may lead to an increased incidence
of atherosclerosis . The recommendation for a low-fat/high-carbohydrate
diet, which is high in trans fatty acids due to margarine intake, leads to
decreases in EFA. Since the standard American diet falls considerably short
of the 6–11 servings of cereal grains recommended by the USDA , it is
unlikely that cereal grain consumption, by itself, adversely inﬂuences the EFA
status of the average American omnivorous diet. However, there are world
populations in which excessive cereal grain consumption clearly has a deleteri-
ous impact upon essential fatty acid status. Studies of vegetarian and nonvege-
tarian populations from the Indian subcontinent who derive the bulk of their
caloric intake from cereals and pulses have consistently demonstrated high
plasma n-6/n-3 ratios, low levels of 20:5n-3 and 22:6n-3 and high levels of
18:2n6 when compared to western populations [125, 128–130]. Associated with
these altered fatty acid levels is a mortality rate from CHD which is equal to
[36, 37] or higher than [36, 129, 130] that found in western populations.
Although the precise etiology of high levels of CHD in Indian populations is
unclear, reduced plasma levels of n-3 fatty acids likely increase the risk for
CHD by a variety of mechanisms which inﬂuence blood lipids, blood pressure,
blood thrombic tendencies, and cardiac arrhythmias . Since the western
diet is already overburdened by an excessively high (n-6/n-3) ratio from vegeta-
ble oils, margarine and shortening , nutritional recommendations encour-
aging increased cereal grain consumption at the expense of fruits, vegetables,
seafood and lean meats may indirectly contribute to an EFA proﬁle which
There is substantial evidence to show that low-density lipoprotein (LDL)
oxidation plays an integral role in atherogenesis , and that diets enriched in
linoleic acid increase the linoleic acid content of LDL and therefore increase
its susceptibility to oxidation . Blankenhorn et al.  have found that
increased intake of linoleic acid signiﬁcantly increased the risk of developing
new atherosclerotic lesions in human coronary arteries. Further, the linoleic acid
content of adipose tissue has been positively associated with the degree of CHD
in patients undergoing coronary angiography . Because cereal-grain- and
pulse-based diets are quite high in linoleic acid (table 6), populations consuming
these diets have been shown to have elevated plasma levels of linoleic acid when
compared to western populations [125, 129]. It is possible that the high mortality
rates of these populations [36, 129, 130] may be partially attributable to a high
linoleic acid intake which increases the oxidative susceptibility of LDL.
35Cereal Grains: Humanity’s Double-Edged Sword
These facts underscore the importance of a proper dietary balance of not
only the short-chain n-3 and n-6 fatty acids, but of the preformed long-chain
fatty acids of both the n-3 and n-6 families which are only found in foods of
animal and marine origin. A diet based primarily upon cereal grains, legumes
and plant foods inevitably leads to a disruption of this delicate balance among
the dietary fatty acids, and ultimately may alter optimal health via subtle
changes in eicosanoid, prostaglandin, prostacyclin, thromboxane and leukotri-
ene function in various tissues. Human dietary lipid requirements were shaped
eons ago, long before the agricultural revolution, and long before humanity’s
adoption of cereal grains as staple foods. Hence, the lipid composition of diets
based upon cereal grains, legumes, vegetable oils and other plant products is
vastly at odds with that found in wild game meat and organs , the primary,
evolutionary source of lipids to which the human genetic constitution is opti-
mally adapted .
Because human body proteins constantly undergo breakdown and resyn-
thesis during growth, development and aging, there is a dietary need for
protein. Human body proteins are composed of 21 separate amino acids which
are divided into three categories: (1) essential; (2) conditionally essential, and
(3) nonessential. The nine essential amino acids cannot be synthesized in the
body and consequently must be supplied by diet. The conditionally essential
amino acids can be endogenously synthesized, however under certain physio-
logical and pathological conditions, endogenous synthesis is inadequate and
needs must be met by the diet. The nonessential amino acids can be endoge-
nously synthesized under all conditions if there is an adequate dietary source
of usable nitrogen. Consequently, in order for normal human protein metabo-
lism to take place, there must be an adequate dietary intake (qualitative) of
all 9 essential amino acids as well as an adequate intake (quantitative) of
protein for synthesis of the conditionally essential and nonessential amino
acids. The long-term metabolic consequences of imbalanced or marginally
insuﬃcient dietary amino acid intake in humans are not well documented;
however there is evidence which suggests these types of diets can result in
impaired linear growth , losses of body mass, muscular strength and
impaired immune function  as well as impaired recovery from illness 
and surgery .
Table 7 contrasts the amino acid contents of animal food sources to that
in cereal grains and legumes. Inspection of both tables 5 and 7 show that the
essential amino acid, lysine, is consistently lower in cereal proteins compared
to animal proteins. Also, the essential amino acid, threonine, tends to be lower
in cereal-based proteins relative to animal protein sources. The relative protein
Table 5. Amino acid and nutrient composition of eight unprocessed cereal grains (100-
Wheat Maize Rice Barley Sorghum Oats Rye Millet
Essential amino acids
Tryptophan, mg 160 67 101 208 124 234 154 119
(64%) (27%) (40%) (83%) (50%) (94%) (62%) (48%)
Threonine, mg 366 354 291 424 345 575 532 354
(81%) (79%) (65%) (94%) (77%) (128%) (118%) (79%)
Isoleucine, mg 458 337 336 456 433 694 550 465
(71%) (52%) (52%) (70%) (67%) (107%) (85%) (72%)
Leucine, mg 854 1,155 657 848 1,491 1,284 980 1,400
(90%) (122%) (69%) (89%) (157%) (135%) (103%) (147%)
Lysine, mg 335 265 303 465 229 701 605 212
(42%) (33%) (38%) (58%) (29%) (88%) (76%) (26%)
Methionine, mg 201 198 179 240 169 312 248 221
(47%) (46%) (42%) (56%) (40%) (73%) (58%) (52%)
Cystine*, mg 322 170 96 276 127 408 329 212
(76%) (40%) (23%) (65%) (30%) (96%) (77%) (50%)
Phenylaline, mg 593 463 410 700 546 894 673 580
(125%) (97%) (86%) (147%) (115%) (188%) (142%) (122%)
Tyrosine*, mg 387 383 298 358 321 573 339 340
(81%) (81%) (63%) (75%) (68%) (121%) (71%) (72%)
Valine, mg 556 477 466 612 561 937 747 578
(85%) (73%) (72%) (94%) (86%) (144%) (115%) (89%)
Histidine, mg 285 287 202 281 246 405 367 236
(52%) (52%) (37%) (51%) (45%) (74%) (67%) (43%)
Kilocalories 327 365 370 354 339 389 335 378
Protein, % total calories 12.6 9.4 7.9 12.5 11.3 16.9 14.7 11.0
Carbohydrate, % total 71.3 74.1 77.2 73.3 74.4 66.0 69.8 73.0
Fat, % total calories 1.5 4.7 2.9 2.3 3.3 6.9 2.5 4.2
Values in (parentheses) represent RDA %. No detectable amounts of taurine in any grain.
* Conditionally essential amino acids.
content of cereal grains averages 12.0% (table 5) whereas that in lean beef is
22%. Consequently, a higher total intake of cereal products would be required
to meet the needs for both total protein and certain individual essential amino
acids when compared to animal foods.
Table 8 clearly indicates that cereal grains provide the majority of protein
calories for most countries of the world. Because cereal-based diets frequently
37Cereal Grains: Humanity’s Double-Edged Sword
Table 6 . Fatty acid content of cereal grains (g fatty acid/100-gram sample): adapted
from Weihrauch et al. 
Fatty acid Wheat Maize Rice Barley Sorghum Oats Rye Millet
14:0 – 0.00 0.03 0.01 0.01 0.02 – 0.00
16:0 0.36 0.40 0.54 0.45 0.44 1.21 0.25 0.68
18:0 0.01 0.06 0.04 0.02 0.03 0.10 0.02 0.16
20:0 – 0.01 0.01 0.00 0.00 0.04 0.00 0.02
16:1 0.01 0.01 0.01 0.01 0.04 0.02 0.01 0.02
18:1 0.25 0.91 0.54 0.24 1.15 2.60 0.22 0.83
18:2n-6 1.20 2.12 0.78 1.14 1.46 2.87 0.95 1.69
18:3n-3 0.10 0.03 0.03 0.13 0.09 0.16 0.12 0.13
Ratio (n-6/n-3) 12.0 70.7 26.0 8.7 16.2 17.9 7.9 13.0
Fat, % total calories 2.7 4.1 2.3 2.8 3.3 7.4 2.2 4.1
include legumes and small amounts of animal protein, they are almost always
adequate in the qualitative aspect of amino acid nutriture ; however the
possibility exists that lysine intake may be marginal , particularly in
children receiving a single or limited number of food protein choices .
Although cereal- and legume-based diets are usually adequate in the
qualitative aspects of amino acid nutriture, there is evidence that under some
circumstances they may fall short in quantitative aspects. The current estimated
mean dietary protein requirements for healthy adult men and women of all
ages is 0.6 g/kg/day, with a suggested safe protein intake set at 0.75 g/kg/day
by the Joint FAO/WHO/UNU Expert Consultation  and at 0.8 g/kg/day
by the Food and Nutrition Board of the US National Research Council .
There is now considerable evidence to suggest that these recommendations
are too low for both adults [144, 145] and the elderly  and that safe
Table 7 . Amino acid distribution in cereal, legume and animal food sources: adapted
from Young et al. 
Food Lysine content Sulfur amino acids Threonine Tryptophan
mg/g protein mg/g protein mg/g protein mg/g protein
Cereal grains 31×10 37×532×412×2
Legumes 64×10 25×338×312×4
Animal foods 85×938 44 12
Table 8 . Nutritional contributions of cereal grains to various regions of the world:
adapted from Young et al. 
Region Caloric intake Caloric intake Protein intake Protein intake
g from cereals g from cereals
North America 3,557 17 105.7 18
Western Europe 3,376 26 94.8 29
Eastern Europe and USSR 3,481 38 103.3 37
Latin America 2,557 39 65.5 38
Africa 2,205 47 55.0 51
Near East 2,620 61 73.5 62
Far East 2,029 67 48.7 63
All developed countries 3,395 31 99.1 30
All developing countries 2,260 61 57.3 55
World 2,571 50 68.8 45
dietary protein intakes may be as high as 1.0–1.25 g/kg/day [145, 146]. The
elderly are particularly vulnerable to inadequate protein intakes. A nutritional
survey of 946 free-living men and women in the United States over the age
of 60 years showed that approximately half of them consumed less than
1.0–1.25 g/kg/day of protein . Because the total protein content of cereal
grains is considerably less than that in animal-based foods (table 7), the dis-
placement of animal foods by excessive consumption of cereal grains has the
potential to compromise adequate protein intake. Indeed, only 2 of 8 elderly
Brazilian men consuming their typical rice and bean diet (containing 0.63 g/
kg/day protein) were able to achieve positive nitrogen balance . Because
cereal-grain-based diets provide at least 50% of the protein calories for the
world population, it is quite likely that inadequate protein intake in the elderly
may be quite common [137, 146, 148].
Although taurine is considered a conditionally essential amino acid, there
is increasing recognition that humans have limited ability to synthesize taurine
39Cereal Grains: Humanity’s Double-Edged Sword
Table 9 . Diseases which may occur
simultaneously with celiac disease Addison’s disease
Autoimmune thyroid diseases
Dental enamel defects
Epilepsy with cerebral calciﬁcations
Insulin-dependent diabetes mellitus
Chronic active hepatitis
Primary sclerosing cholangitis
Primary biliary cirrhosis
Selective IgA deﬁciency
Systemic lupus erythematosus
from cysteine [149, 150], consequently dietary taurine plays an important role
in maintaining body taurine pools [151, 152]. All plant foods have undetectable
amounts of taurine  including cereal grains (table 5). Studies of vegans
have shown them to maintain lower levels of both plasma and urinary taurine
[154, 155]. The clinical sequelae of long-term taurine deﬁciency in individuals
consuming cereal- and plant-based diets has not been studied. However, tau-
rine is known to positively inﬂuence cardiovascular disease by reducing platelet
aggregation , by reducing reperfusion injury via free radical scavenging
action , and by exhibiting antiarrhythmic activity . Furthermore,
taurine appears to have an essential role in the posttrauma state [159, 160]
and in maintaining normal retinal function .
Consistent with populations from the fossil record showing a characteristic
reduction in stature with the adoption of cereal-based agriculture [4, 17–19],
is the observation that present-day populations depending upon cereal grains
for the bulk of their energy and protein also tend to be of short stature
[162–165]. Further, vegan and vegetarian children often fail to grow as well
as their omnivorous cohorts despite apparently adequate intakes of amino
acids and nitrogen . There are a variety of reasons why cereal-based diets
may impair linear growth. These include deﬁciencies in energy, protein, zinc,
iron, copper, calcium, vitamin D, vitamin B
and vitamin A [136, 166]. How-
ever, for none of these nutrients is there clear, consistent evidence that
supplementation with the nutrient beneﬁts linear growth . It is likely that
Fig.3. Pathogenesis of childhood urinary bladder stones. Adapted from Teotia et al. .
growth and hence adult stature is limited by multiple, simultaneous deﬁciencies
 in populations dependent upon cereal grains for the bulk of their caloric
intake. Excessive consumption of cereal grains clearly has a deleterious eﬀect
upon virtually all of the previously listed nutrients.
Childhood urinary bladder stones have virtually disappeared from western
countries, however they are still very common in developing countries such as
Pakistan, India, Thailand, Sumatra, Taiwan and Iran [167–170]. These stones
are composed primarily of ammonium acid urate, and studies of children in
these areas have demonstrated increased urinary excretion of oxalate ammonia
and uric acid and decreased urinary phosphate and pH; factors which strongly
favor ammonium urate calculi [168, 171]. It has been shown that an increase in
urinary ammonia occurs in babies whose feeds consisted predominantly of rice
. Furthermore, urinary bladder stones have been reported to be common
in Australian aboriginal children in which breast feeding is supplemented with
white ﬂour and little else . Bladder stone disease in children was endemic
in 19th-century England, and it has been suggested that the exclusive substitu-
tion of breast milk with porridge and bread was a signiﬁcant factor in the patho-
genesis of this disease . Endemic childhood bladder disease clearly occurs
in countries and populations in which cereal grains comprise most of the caloric
41Cereal Grains: Humanity’s Double-Edged Sword
intake, and cereal grains have been implicated in the etiology of the disease [168,
172]. However, it is likely that other factors, including calorie and protein malnu-
trition, infection and starvation operate synergistically with high intake of cereal
grains to elicit the disease  (ﬁg. 3).
Antinutrients in Cereal Grains
In the evolution of plant life history strategies, plant species encounter a
basic dilemma in the amount of adaptational energy they must allocate to
growth versus that which they must allocate to defenses necessary for survival
in the presence of pathogens and herbivores . Therefore, plants face an
evolutionary tradeoﬀ; they must grow fast enough to compete, yet they must
also divert enough energy for the synthesis of secondary metabolites required
to ward oﬀpathogens and herbivores. Defense is not the only role of secondary
metabolites, and other functions include attraction of pollinators, protection
from ultraviolet light, structural support, temporary nutrient storage, phyto-
hormone regulation, facilitation of nutrient uptake and protection of roots
from acidic and reducing environments . Quite frequently, plants provision
seeds with high concentrations of secondary metabolites to ensure the survival
of the seed and the rapidly growing seedling before it can synthesize its own
Cereal grains which are the seeds of grasses (gramineae) contain a variety
of secondary metabolites which can be either toxic, antinutritional, benign or
somewhere in between, dependent upon the physiology of the consumer an-
imal. The presence of secondary metabolites in plants do not guarantee free-
dom from predation by herbivores, and many herbivores have evolved a number
of strategies for circumventing the resistance mechanisms of their hosts .
Many birds, rodents, insects and ruminants can clearly consume cereal grains
in high quantities with minimal undue eﬀects. Because primates evolved in
the tropical forest, all of their potential plant food was derived from dicotyle-
donous species; therefore, the primate gut was initially adapted to both the
nutritive and defensive components of dicotyledons rather than the nutritive
and defense components of monocotyledonous cereal grains . Under
certain conditions a few species of primates (Papio species, Theropithecus
gelada) have been observed to consume grass and grass seeds; however, by
and large, consumption of monocotyledonous plant foods, particularly cereal
grains, is a notable departure from the traditional plant foods consumed by
the majority of primates . Consequently, humans, like all other primates
have had little evolutionary experience in developing resistance to secondary
and antinutritional compounds which normally occur in cereal grains.
Alkylresorcinols are phenolic compounds which are found in the highest
amounts in rye (97 mg/100 g), in high amounts in wheat (67 mg/100 g) and
in lower amounts in other cereals such as oats, barley, millet and corn .
These compounds are concentrated in the outer bran layers of cereal grains
and are thought to provide resistance from pathogenic organisms during dor-
mancy and germination . Alkylresorcinols previously were associated only
with rye and were thought to be a problem only in animal nutrition. Feeding
of rye in large amounts to cattle, sheep, horses, pigs and poultry has been
shown to cause slower growth than feeding of other cereal grains . Sub-
sequent studies indicated the growth depressive eﬀects of alkylresorcinols could
be attributed to both an appetite depressive eﬀect (70%) and a direct toxic
eﬀect (30%) .
Although there is scant information upon the eﬀects of alkylresorcinols
in human nutrition, in animal models they have been shown to cause red-cell
blood hemolysis, permeability changes of erythrocytes and liposomes, DNA
strand scission, and have been shown to be involved in many pathological
conditions including hepatocyte and renal degeneration . An in vitro
experiment in humans has shown that alkylresorcinols were able to stimulate
platelet thromboxane (TXA
) production by 30–65% using 0.02–2.0 mmol/l
concentrations. To date no human experiments have been conducted to deter-
mine if these proinﬂammatory eﬀects can occur in vivo from alkylresorcinols
ingested from whole grain wheat products. It should be pointed out that cereal
grain alkylresorcinols may have antimutagenic activity , and in lower
concentrations may have antioxidant properties .
The aqueous/saline protein extract of wheat seed is called the albumin
fraction. Within the albumin fraction are a very large number of protein
components capable of inhibiting alpha-amylases from insect, mammalian,
avian and marine species. Alpha-amylase inhibitors make up as much as 80%
of the total albumin fraction and may represent 1% of wheat ﬂour .
Because of their thermostability, alpha-amylase inhibitors persist through
bread baking and are found in large amounts in bread, breakfast cereals, pasta
and other wheat products . Alpha-amylase inhibitors are ubiquitous in
the cereal family (gramineae) and in addition to their presence in wheat, they
have been found in rye, barley, oats, rice and sorghum. As with alkylresorcinols,
alpha-amylase inhibitors are thought to have evolved in cereal grains as a
defense mechanism against herbivore predation, primarily against insects .
The multiple alpha-amylase inhibitors found in cereal grains have distinc-
tive structural properties and show considerable variability in their inhibitory
43Cereal Grains: Humanity’s Double-Edged Sword
eﬀect upon human salivary and pancreatic alpha-amylase [185, 186]. Because
salivary and pancreatic amylases catalyze the hydrolysis of glycosidic linkages
in starch and other related polysaccharides, their inhibition by cereal grain
alpha-amylase inhibitors have been theorized to have beneﬁcial therapeutic
eﬀects by reducing carbohydrate-induced hyperglycemia and hyperinsulinemia
. Early studies of commercially available alpha-amylase inhibitor prepara-
tions failed to decrease starch digestion in humans [188, 189] perhaps because
of insuﬃcient antiamylase activity . More recent research utilizing puriﬁed
amylase inhibitors have demonstrated that these antinutrients can rapidly
inactivate amylase in human intestinal lumen [186, 190] in a dose-dependent
manner  and reduce postprandial rises in glucose and insulin .
Although the acute eﬀects of alpha-amylase inhibitors may appear to
have therapeutic beneﬁt in patients suﬀering from diabetes mellitus, obesity
and other diseases of insulin resistance, chronic administration in animal
models has been shown to induce adverse eﬀects including deleterious histo-
logical changes to the pancreas and pancreatic hypertrophy . Because it
is unclear if these dietary antinutrients can elicit similar deleterious changes
in the pancreatic structure and function of humans , the presence of
alpha-amylase inhibitors in human foodstuﬀs is generally considered to be
In addition to their inﬂuence upon starch digestion, alpha-amylase inhib-
itors are known to be prominent allergens. The inhalation of cereal ﬂours is
the cause of baker’s asthma, an occupational allergy with a high prevalence
in the baking industry . Baker’s asthma is mediated by IgE antibodies,
and until recently the identiﬁcation of the IgE binding proteins (allergens) in
the putative cereal ﬂours was unknown. Over the past decade, it has been
conclusively demonstrated that a variety of alpha-amylase inhibitor proteins
are responsible for bakers’ allergenic reaction to cereal ﬂours [194, 195]. Fur-
ther, alpha-amylase inhibitors recently have been demonstrated to be a relevant
allergen in children experiencing hypersensitivity reactions following wheat
Protease inhibitors are proteins which have the ability to inhibit the proteo-
lytic activity of certain enzymes and are common throughout the plant king-
dom, particularly among the legumes. As with alpha-amylase inhibitors, there
are a muiltiplicity of plant proteins which have protease inhibitor activity.
The two best-studied protease inhibitors, derived from plants, are the Kunitz
inhibitor, which has a speciﬁcity directed mainly towards trypsin in human
gastric juice, and the Bowman-Birk inhibitor which is capable of inhibiting
chymotrypsin as well as trypsin. The Bowman-Birk inhibitor is relatively stable
to both heat and digestion and can therefore survive intact through cooking
and transit through the stomach .
Normally, there is a negative feedback loop whereby the secretory activity
of the pancreas is controlled by the level of trypsin in the intestinal tract.
Intraluminal trypsin inhibits pancreatic secretion by inhibiting the release of
the hormone cholecystokinin from the intestinal mucosa; however when die-
tary protease inhibitors bind trypsin, there is an uncontrolled release of chole-
cystokinin. This continuous and excessive release of cholecystokinin has been
shown in animal models to result in pancreatic hypertrophy and hyperplasia
 and may eventually lead to cancer . The deleterious inﬂuence of
the Bowman-Birk inhibitor upon this negative feedback loop has been demon-
strated in humans .
As with other secondary metabolites, the primary function of protease
inhibitors in plants is thought to prevent predation from invading insects and
microbes . Protease inhibitors have been found in virtually all of the
cereal grains ; however, they apparently have low trypsin inhibitory activ-
ity. Wheat has been shown to have only 1.5% the trypsin inhibitory activity
of soy beans . Nonetheless, feedings of raw rice bran  and raw rye
and barley  have resulted in pancreatic hypertrophy in broiler chicks
which was attributable to protease inhibitors. In humans, the dietary eﬀects
of chronic low level exposure to plant protease inhibitors are unknown, and
there is some evidence that they may have beneﬁcial, antineoplastic eﬀects
Lectins are proteins that are widespread in the plant kingdom with the
unique property of binding to carbohydrate-containing molecules, particularly
toward the sugar component. They were originally identiﬁed by their ability
to agglutinate (clump) erythrocytes which occurs because of the interaction
of multiple binding sites on the lectin molecule with speciﬁc glycoconjugate
receptors on the surface of the erythrocyte cell membranes. Because of this
binding property, lectins can interact with a variety of other cells in the body
and are recognized as the major antinutrient of food .
Of the eight commonly consumed cereal grains, lectin activity has been
demonstrated in wheat, rye, barley, oats, corn , and rice  but not in
sorghum or millet . The biological activity of lectins found in cereal grains
are similar because they are closely related to one another both structurally
and immunologically . The best studied of the cereal grain lectins is wheat
germ agglutinin (WGA), and the in vitro biological eﬀects of WGA upon
tissues and organs are astonishingly widespread. Virtually every cell in the
body, and every extracellular substance can be bound by WGA because of
45Cereal Grains: Humanity’s Double-Edged Sword
the ubiquity of secreted glycoconjugates . In his comprehensive review,
Freed  has shown that WGA can bind (in vitro) the following tissues and
organs: alimentary tract (mouth, stomach, intestines), pancreas, musculoskele-
tal system, kidney, skin, nervous and myelin tissues, reproductive organs, and
platelets and plasma proteins.
WGA is heat stable and resistant to digestive proteolytic breakdown in
both rats  and humans  and has been recovered intact and biologically
active in human feces . WGA and lectins in general bind surface glycans
on gut brush border epithelial cells, and the damage they cause to these cells
interferes with digestive/absorptive activities, stimulates shifts in bacterial ﬂora
and modulates the immune state of the gut . In rats, WGA has been
shown to cause hyperplastic and hypertrophic growth of the small intestine
and interfere with normal gut metabolism and function, while simultaneously
inducing pancreatic enlargement and thymic atrophy . The dietary levels
of WGA (7 g/kg body weight) necessary to induce these untoward eﬀects in
rats is signiﬁcantly higher than dietary levels of WGA which would be normally
encountered in foods derived from wheat, since the concentration of WGA is
about 2 g/kg in unprocessed wheat germ . No long-term studies of low
level WGA ingestion upon gut structure and function have been conducted
in humans; however there is suggestive evidence that high wheat gluten diets
induce jejunal mucosal architectural changes in normal subjects without celiac
Most food proteins entering the small intestine are fully degraded into
their amino acid components and therefore do not pass intact into systemic
circulation. However, it is increasingly being recognized that small quantities
of dietary protein which escape digestive proteolytic breakdown can be syste-
mically absorbed and presented by macrophages to competent lymphocytes
of the immune system [215, 216]. Under normal circumstances, when the
luminal concentrations of intact dietary proteins is low, absorbed proteins
generally elicit a minimal allergic response because of the limiting inﬂuence
of T-suppressor cells. Because of their resistance to digestive, proteolytic break-
down, the luminal concentrations of lectins can be quite high, consequently
their transport through the gut wall can exceed that of other dietary antigens
by several orders of magnitude . Additionally, WGA and other lectins,
may facilitate the passage of undegraded dietary antigens into the systemic
circulation by their ability to increase the permeability of the intestine .
Consequently, dietary lectins represent powerful oral immunogens capable of
eliciting speciﬁc and high antibody responses . In rats, dietary WGA is
rapidly transported across the intestinal wall into systemic circulation where
it is deposited in blood and lymphatic vessel walls . Although no direct
human experiments have been conducted evaluating dietary WGA passage
into systemic circulation, there is substantial evidence to indicate that this
event occurs since serum antibodies to WGA are routinely found in normals
[218, 219] and in celiac patients .
Once WGA crosses into systemic circulation, it has the potential to inter-
fere with the body’s normal hormonal balance, metabolism and health [210,
213]. Numerous in vitro studies have shown WGA to have insulomimetic
eﬀects [220, 221]. Although few animal and no human studies have been
designed to evaluate the in vivo inﬂuence of dietary WGA upon insulin metabo-
lism, experiments utilizing dietary kidney bean lectin (PHA) in rats have
demonstrated a depression in circulating insulin levels which modulates com-
plex change in the body’s hormonal balance . Numerous in vitro studies
suggest that WGA may have the potential to subtly impact health via its ability
to inhibit the mitogenic actions of multiple peptide growth factors including
insulin-like growth factor (IGF) , platelet-derived growth factor ,
epidermal growth factor [222, 223] and nerve growth factor . Children
with celiac disease exhibit short stature and stunted growth patterns ,
depressed levels of IGF-I [226–228], depressed levels of IGF-binding protein
3 (IGFBP-3) [226, 227] and lower levels of growth hormone binding protein
II (GH-BP II) . Administration of wheat (gluten)-free diets in celiac
children increases circulating levels of IGF-I [226, 227], IGFBP-3 [226, 228]
and GH-BP II  while simultaneously improving height and weight .
Presently, there is insuﬃcient data in humans to determine the health ramiﬁca-
tions of chronic low level consumption of WGA, but because detectable
amounts of functionally and immunochemically intact WGA are transported
across the intestinal wall , the potential for this lectin to disrupt human
health is high.
Autoimmune Diseases and Cereal Grain Consumption
Autoimmune diseases occur when the body loses the ability to discriminate
self proteins from nonself proteins. This loss of tolerance ultimately results in
destruction of self tissues by the immune system. Autoimmune diseases occur
in a variety of tissues and include such well-known maladies as rheumatoid
arthritis, multiple sclerosis, and insulin-dependent diabetes mellitus (IDDM).
Typically, autoimmune diseases are characterized by the presence of autoanti-
bodies against speciﬁc self proteins . Most autoimmune diseases are
thought to develop via an interaction of an environmental factor or factors
in conjunction with a speciﬁc hereditary component.
Dietary cereal grains are the known environmental causative agent for at
least two autoimmune diseases: celiac disease  and dermatitis herpeti-
47Cereal Grains: Humanity’s Double-Edged Sword
formis . Withdrawal of gluten-containing cereals from the diet ameliorates
all symptoms of both diseases. Further, evidence from clinical, epidemiological
and animal studies implicate cereal grains in the etiology of other autoimmune
diseases. The mechanism or mechanisms by which cereal grains may induce
autoimmunity in genetically susceptible individuals is not clearly deﬁned;
however it is increasingly being recognized that the process of molecular
mimicry, by which a speciﬁc foreign antigen may cross react with self antigens,
may be involved in a variety of autoimmune diseases [232, 233]. Additionally,
cereal grain lectins and proteins may also have involvement in the development
of autoimmunity via their modulation of immune system components [234,
The development of autoimmunity is a poorly understood process; how-
ever it is generally agreed that it occurs as a result of an interaction between
environmental and genetic components . The genetic component most
closely associated with the expression of autoimmune diseases are those genes
which code for the human leukocyte antigens (HLA). The HLA is subdivided
into class I (HLA-A, HLA-B, HLA-C), class II (HLA-DR, HLA-DQ and
HLA-DP) and class III categories. Both class I and class II proteins are
transmembrane cell surface glycoproteins which are required for the recogni-
tion of both self and foreign antigens by T lymphocytes. Class I proteins are
found on all nucleated cells and platelets, whereas class II HLAs are found
on macrophages, monocytes, epithelial dendritic cells, B lymphocytes and
activated T lymphocytes. Class I HLA proteins present peptide fragments
from degraded intracellular viruses to circulating CD8+cytotoxic lympho-
cytes which recognize and attack virus-infected cells. Class II HLA proteins
present foreign antigens to CD4+T lymphocytes which results in the induction
of T-cell proliferation, lymphokine production, and subsequent synthesis of
immunoglobulin by B lymphocytes. Except for human spondyloarthropathies,
the preponderance of known or suspected autoimmune diseases are associated
with class II haplotypes .
Many tissues (thyroid, adrenal, pancreatic islet beta cells, bile ducts, kidney,
etc.) that are typically attacked by autoimmune diseases do not normally express
class II HLA antigens, consequently, it is paradoxical that autoimmunity should
developinthesetissues.Theinductionofinappropriateclass IIantigens innucle-
ated cells may be an important preliminary event in the etiology of autoimmune
disease  and can occur from the stimulatory eﬀect of interferon-c(IFN-c)
wrought by viral infections . Additionally, lectins are potent inducers of
HLA class II molecules , probably via their ability to stimulate release of
IFN-c[238, 239]. Further, the gliadin fraction of wheat, which exhibits lectin
activity , has been shown to amplify HLA class II expression in intestinal
epithelial cell lines . Ingested WGA from dietary wheat products, crossing
the intestinal barrier would also inﬂuence the development ofautoimmunity by
its ability to stimulate T-lymphocyte proliferation [234, 241].
In autoimmune disease, the inability of the immune system to distinguish
self antigens from foreign antigens ultimately results in the destruction of self
tissues. Thereis now a substantial body ofevidence indicating that the breaking
of tolerance to self antigens can occur when invading foreign proteins contain
amino acid homologies similar to a protein in thehost [233, 242]. This similarity
in structure shared by products of dissimilar genes (dubbed molecular mimicry)
causes cross-reactive immune responses which are directed not only at the
invading foreign protein but also at any cells displaying amino acid sequences
similar to those of the foreign protein. The main body of evidence implicates
viral and bacterial pathogens as initiators of cross reactivity and autoimmunity
[233, 242]; however there is an emerging body of literature supporting the
view that dietary antigens [243, 244], including cereal grains [245, 246], may
also induce cross-reactivity and hence autoimmunity by virtue of peptide
structures homologous to those in the host.
Genetic and Anthropological Factors
Virtually all autoimmune diseases have a strong genetic component cate-
gorized by a variety of HLA haplotypes . For instance, there is a 73%
greater risk of developing celiac disease in people displaying the HLA-DQ2
antigen relative to those who do not . It is not entirely clear why HLA
genes alter the relative risk for autoimmune disease; however it is likely that
they inﬂuence the binding aﬃnity of the HLA peptide complex with circulating
T lymphocytes. Because the protein subunits comprising the HLA antigen
binding groove are coded by highly polymorphic HLA genes , various
HLA alleles can subtly alter the structure of the HLA antigenic binding groove
 and therefore inﬂuence whether a mimicking epitope has a proliferative
or anergizing response upon engagement of the HLA peptide complex with
the T-cell receptor. From an evolutionary perspective, the inheritance of speciﬁc
HLA haplotypes appears to be primarily related to infectious disease suscepti-
bility, and inheritance of certain HLA haplotypes may have conferred relative
protection from invading pathogens [248, 249].
In celiac disease, there is a general geographical northwest (NW) to south-
east (SE) disease incidence gradient from the Near East to Northern Europe
. Associated with this gradient is a concurrent NW/SE gradient for the
HLA-B8 antigen which parallels the spread of agriculture and hence cereal
49Cereal Grains: Humanity’s Double-Edged Sword
Fig. 4. HLA-B8 frequencies and the spread of agriculture in Europe. Adapted from
grain consumption (wheat and barley) from the Near East 10,000 years ago
(ﬁg. 4). HLA-B8 is not a direct marker for celiac disease, but because it is in
linkage disequilibrium with HLA-DQ2, it is directly implicated with the dis-
ease. Consequently, high frequencies of HLA-B8 (which are positively associ-
ated with celiac disease via their close linkage with HLA-DQ2) occur in
European populations with the least evolutionary exposure to cereal grains,
and conversely, those populations with the most evolutionary exposure to
cereal grains maintain lower frequencies of HLA-B8 [249, 250]. It has been
suggested that this gradient occurs because high frequencies of HLA-B8 and
hence HLA-DQ2 were once typical of Near Eastern peoples; however these
antigens became a liability with the advent of regular cereal grain consumption
ushered in by the agricultural revolution [249, 250]. Because cereal grain
consumption presumably would have increased mortality (via increased suscep-
tibility to celiac disease) in populations with HLA-DQ2, natural selection
would have reduced the frequency of this antigen in populations with the most
evolutionary exposure to wheat and barley [249, 250].
Because of the strong linkage disequilibrium for the genes which code for
the (B8, DR3, DQ2) haplotype, autoimmune disorders linked with DR3,
including IDDM, have been found more often in celiac disease patients .
The incidence of IDDM is approximately 7–10 times higher in celiacs than
in the normal population [252, 253], and the incidence of IDDM, like celiac
disease, is found in Europe in a general NW/SE gradient . Both milk
[243, 255] and wheat , contain dietary components which would have
increased in European populations adopting agriculture, and have been sus-
pected elements in the pathogenesis of IDDM.
Autoimmune Diseases Associated with Cereal Grain Consumption
There are a number of autoimmune diseases in which cereal grains have
been implicated. In a few of these diseases (celiac disease and dermatitis
herpetiformis), there is a 100% certainty that cereal grains are the causative
agent, whereas in others the link is not so strong. Because of the increased
incidence  of other, simultaneously occurring autoimmune diseases in
celiac patients (table 9), many of these maladies have been examined to deter-
mine, what role, if any, cereal grains may play in their etiology.
Celiac Disease. Marsh  stated: ‘Despite the central importance of
wheat as a dietary staple throughout the world, it is astounding that its
presumptive role in precipitating celiac sprue disease was discovered only 40
years ago by the Dutch pediatrician W.K. Dicke.’ Indeed, it is ‘astounding’
that humanity was unaware, until only relatively recently, that an ordinary
and commonplace food such as cereal grains could be responsible for a disease
which aﬄicts between 1 and 3.5 people per 1,000 in Europe . The precise
mechanism by which certain peptide sequences in the alcohol-soluble fraction
(gliadin) of wheat, rye and barley elicit celiac disease is still poorly understood
. However, there is an increasing consensus that celiac disease is an
autoimmune disease [230, 259], mediated by T lymphocytes within the lamina
propria which damage intestinal villi.
It is probable that the process of molecular mimicry is involved in the
development of celiac disease . Kagnoﬀet al.  have shown that wheat
alpha-gliadin shares an amino acid sequence homology with the E1B protein
of human adenovirus 12 (Ad-12) and that antibodies directed against E1B
cross-react with alpha-gliadin. Since 89% of patients with celiac disease, versus
17% of controls, showed evidence of Ad-12 infection , it is possible that
51Cereal Grains: Humanity’s Double-Edged Sword
Ad-12 infection in individuals genetically predisposed to celiac disease (HLA-
DQ2) may facilitate development of the disease by virtue of cross-reactivity,
perhaps by three-way mimicry among the two foreign antigens (Ad-12, gliadin),
the target tissue and even HLA proteins, themselves .
Celiac disease is typically screened by detection of circulating IgG antibod-
ies to reticulin (ARA), endomysium (AMA) or gliadin (AGA). Endomysium
is the connective tissue surrounding smooth muscle ﬁbers of the gut, whereas
reticulin are ﬁbrils connecting smooth muscle cells and elastic tissue within
endomysium. The speciﬁc protein or proteins (autoantigen) within reticulin
and endomysium to which ARA and AMA are directed is unclear; however
recent studies have indicated both transglutaminase  and calreticulin 
are likely candidates. It has been shown that gliadin and calreticulin share
homologous amino acid sequences with one another, and anticalreticulin anti-
bodies cross react with gliadin , thereby supporting the concept that celiac
disease involves molecular mimicry . Because gliadins are a complex
mixture of proteins that contain at least 40 diﬀerent components in a single
variety of wheat , it is unlikely that a single gliadin protein causes celiac
disease, but rather several prolamines that express similar or identical epitopic
domains . Thus, it is likely that multiple gliadin proteins can cross react
with at least one and probably more autoantigens in celiac disease, similar to
that observed in other autoimmune diseases . The self antigen with the
closest molecular structure (following HLA presentation) to the mimicking
foreign peptide will likely be primarily responsible for the destructive auto-
immune response wrought by T lymphocytes.
A general overview of celiac disease would then suggest that dietary WGA
bound to enterocytes increases the permeability of the gut , thereby
allowing entry of both WGA  and other gliadin proteins into systemic
circulation. WGA or perhaps gliadin, by virtue of their lectin properties, induce
the inappropriate expression of HLA class II molecules, which may present
a variety of internally processed proteins (including calreticulin), on the surface
of intestinal epithelial cells . In genetically susceptible individuals (HLA-
DQ2), the molecular conformation of the HLA antigenic binding groove is
subtly altered  so that the presentation of the internally processed, mimick-
ing protein (calreticulin) causes a proliferative rather than anergizing response
upon engagement with the T-cell receptor. Circulating gliadin proteins are
engulfed by macrophages which then present the processed gliadin peptide
fragments, viaHLA molecules, to CD4+T lymphocytes. Because these gliadin
peptide fragments presented by the macrophage have amino acid sequences
homologous to those of the endogenous protein (calreticulin), which is artiﬁ-
cially expressed upon the surface of intestinal epithelial cells by cereal grain
lectin stimulation, cytotoxic CD4+T lymphocytes initiate an immune re-
sponse both upon the macrophage expressing fragments of the foreign peptide
(gliadin) as well as upon the intestinal epithelial cell expressing the homologous,
endogenous protein (calreticulin). Viruses suspected of causing autoimmune
disease operate in a likewise manner to induce the inappropriate expression
of autoantigens, including calreticulin  on the cell surface, as well as
maintaining structural homology to a self antigen [233, 242]. Once the mimicry
process begins, the destructive autoimmune response may be further enhanced
by the ability of WGA [234, 241] or viruses  to induce T-cell proliferation,
mediated by either lectin [238, 239] or viral  IFN-cstimulation.
Dermatitis Herpetiformis. Dermatitis herpetiformis (DH) is characterized
as an intensely itching papulovesicular skin disease diagnosed by IgA deposits
in the basement membrane . DH can be successfully treated by a gluten-
free diet, although it may take years before the dermatitis is fully controlled
by diet only . DH and celiac disease share a common genetic basis (HLA-
DQ2), and approximately 60% of DH patients have moderate to severe small-
bowel villous atrophy . As with celiac disease, the precise tissue autoan-
tigen in DH is unclear. However, there are similar structural homologies
between human elastin and high-molecular weight glutenin (a wheat gluten
protein) which have been shown to cause IgA cross-reactivity of the two
proteins in human serum . Bodvarsson et al.  have suggested that
DH may be due in part to this cross-reactivity (mimicry) between dietary
glutenin and dermal elastin.
Insulin-Dependent Diabetes mellitus. IDDM is a complex disease involving
numerous putative environmental factors; however it has been suggested that
shared amino acid sequences (i.e. molecular mimicry) between viral proteins
and pancreatic beta-cell proteins (e.g. coxsackie virus protein and glutamate
decarboxylase) represent a likely mechanism causing the disease . In
addition to viral proteins, dietary proteins in cow’s milk cross react with a
beta-cell antigen and are therefore suspected environmental etiologic agents
. However, as pointed out by Schatz and Maclaren , the feeding of
wheat in animal models of IDDM elicits a greater incidence of the disease
than does milk. Numerous studies have demonstrated that feeding of wheat
gluten to rats or mice, which are genetically predisposed to IDDM, increases
the expression of the disease [255, 271, 272]. It remains elusive how wheat
proteins increase the expression of IDDM in genetically predisposed animals.
Because Ro/SS-A autoantibodies are found in nonobese (NOD) diabetic mice
 and in humans with IDDM  and in humans with both IDDM
and Sjogren’s syndrome , the molecular mimicry which occurs between
calreticulin and wheat gliadin peptides  may be involved in the auto-
immune response. Although there is conﬂicting data regarding calreticulin’s
role in the Ro/SS-A complex , recent evidence unequivocally shows that
53Cereal Grains: Humanity’s Double-Edged Sword
calreticulin exists in a form directly associated with all four varieties of human
Ro/SS-A RNA molecules .
¨gren’s Syndrome. Sjo
¨gren’s syndrome is an autoimmune disease charac-
terized by lymphocytic inﬁltration of CD4+T cells into salivary and lachrymal
glands leading to symptomatic dry eyes and mouth . Circulating antibody
levels of gliadin and a reticulin glycoprotein have been found to be higher in
patients with Sjo
¨gren’s syndrome than in controls . Furthermore, Sjo
syndrome occurs at a level approximately 10 times higher in celiac subjects than
in normals . Ro/SS-A autoantibodies are typically elevated in Sjo
syndrome [275, 278], and because the four cytoplasmic RNA components of
Ro/SS-A (hY RNA 1,3,4,5) exist together with a form of calreticulin ,
the molecular mimicry between alpha-gliadin and calreticulin  may in
part be responsible for the autoimmune response. Calreticulin is normally a
cytolsolic protein, however viral infection has been shown to increase its cell-
surface expression . In a similar manner, lectins (including gliadin) are
known to induce inappropriate expression of HLA class II molecules at nucle-
ated cell surfaces [235, 237].
¨gren’s syndrome an additional suspected autoantigen, termed
BM180, has been isolated from basement membrane in the lacrimal and parotid
exocrine secretory glands, and which cross-reacts with alpha-gliadin proteins
. Astonishingly, BM 180 contains an N-terminal amino acid sequence
(VRVPVPQLQPQNP) identical to that found in alpha-gliadin, and mono- and
polyclonal antibody data therefore suggest that BM 180 is a mammalian form
ofgliadin . Because BM 180may be required forstimulussecretingcoupling
by lacrimal acinar cells , autoimmune attacks by CD4+T cells, primed
by previous interaction with macrophages presenting alpha-gliadin, would be
directed, via molecular mimicry, at lacrimal and parotid cells inappropriately
presenting BM 180. Despite the suggestive link between celiac disease and
¨gren’s syndrome, as well as the molecular mimicry evidence, there are scant
clinicaltrialsevaluatingthe eﬀectivenessof gluten-free diets in Sjo
Rheumatoid Arthritis. Rheumatoid arthritis is a complex autoimmune
disease involving numerous environmental and genetic components, and sim-
ilar to a number of other autoimmune diseases is found more often in celiac
patients [251, 281]. Multiple studies of arthritic patients have demonstrated
elevated antibody levels for gliadin [282, 283], and gluten-freee diets have
been shown to be eﬀective in reducing arthritic symptoms in celiac patients
[283–285]. No large clinical trials have been undertaken to speciﬁcally examine
the eﬀectiveness of gluten-free diets in the treatment of arthritis; however there
are numerous case studies reporting alleviation of arthritis symptoms with
grain-free diets [286–289]. Additionally, complete withdrawal of food during
fasting reduces objective and subjective indices of the disease .
Because serum antibodies in arthritic patients recognize the antigen, bo-
vine serum albumin (BSA) from cow’s milk, and since BSA contains homo-
logous amino acid sequences with human collagen type I, Clq, it has been
suggested that molecular mimicry represents a potential mechanism by which
milk consumption may trigger arthritis . In addition to milk, glycine-rich
cell wall protein (GRP 1.8), which is ubiquitous in cereal grains and legumes,
shares signiﬁcant amino acid homology with ﬁbrillar collagen and procollagen
and has been shown to stimulate T cells from the synovial ﬂuid of juvenile
and adult rheumatoid arthritis patients . A third dietary antigen which
may also induce rheumatoid arthritis via molecular mimicry is the alpha-
gliadin component of wheat which shares signiﬁcant amino acid sequences with
calreticulin . Anticalreticulin antibodies have been found in rheumatoid
arthritis patients , and HLA-DR4 molecules from arthritic patients are
known to present a peptide fragment derived from calreticulin . Dietary
antigens from three food sources (milk, grains and legumes) contain multiple
peptides which mimic those found in joint tissue from arthritis patients,
whereas grains and legumes additionally contain lectins which can induce
inappropriate presentation of HLA class II molecules [235, 237], consequently,
future dietary interventions aimed at reducing arthritis symptoms would need
to consider these potential confounding eﬀects.
Other Autoimmune Diseases. IgA nephropathy is the most common form
of primary glomerulonephritis worldwide, and about one quarter of these
patients progress to terminal renal failure 10 years after the apparent clinical
onset . IgA nephropathy is characterized by deposition of circulating IgA-
containing immune complexes (IgAIC) in the mesangium. IgA nephropathy
patients maintain increased intestinal permeability , elevated circulating
antibodies to gliadin [296, 297], and have serum that contains exogenous
lectins which induce interleukin-6 (IL-6), a nephritogenic cytokine . In
rodent models, IgA nephropathy can be induced by gliadin-containing diets
and have been shown to signiﬁcantly increase both gliadin antibodies and
IgA mesangial deposits compared to gliadin-free controls . Humans
following gluten-free diets have shown reduced IgA antigens and reduced
levels of IgAIC, however these diets do not appear to alter the progression
towards renal failure . Amore et al.  have suggested that gliadin,
because of its lectin activity may favor the binding of IgA and IgAIC to
mesangial cells, thereby enhancing both IgA mesangial trapping and in situ
IgA deposit formation.
The cause of recurrent aphthous stomatitis (canker sores) is unknown;
however it is suspected to be mediated by immunological mechanisms inter-
acting with an undeﬁned target tissue . O’Farrelly et al.  have shown
that 4 of 11 aphthous stomatitis patients had raised levels of antibodies to
55Cereal Grains: Humanity’s Double-Edged Sword
alpha-gliadin, and in 3 of these 4 subjects, the ulceration remitted on a
gluten-free diet and relapsed upon gluten challenge. Other studies of aphthous
stomatitis patients have shown favorable responses to gluten-free diets in
some, but not all aphthous stomatitis patients [303, 304]. The mechanism
by which wheat gluten is associated with the development of aphthous ulcera-
tions is unclear.
There is increasing recognition that molecular mimicry is a highly likely
mechanism underlying the development of multiple sclerosis [305, 306]. A
number of viral and bacterial proteins have been shown to cross react with
myelin basic protein (MBP) , one of the suspected target antigens in
multiple sclerosis (MS). Because the blood-brain barrier limits access to the
CNS to activated T cells, invasion of the CNS requires autoreactive T cells
to be stimulated in the peripheral immune system. Therefore, it is possible
that dietary antigens causing persistent T-cell stimulation, and bearing similar
amino acid homologies to the various myelin and nonmyelin target antigens,
could cause polyclonal expansion of autoreactive T cells in the periphery, in
a manner similar to that observed for bacterial and viral antigens. Although
no homologous amino acid sequences have yet been identiﬁed between dietary
antigens and suspected autoantigens in MS patients, there are epidemiological
reports which link both wheat  and milk  consumption to the inci-
dence of multiple sclerosis, consistent with the observations that MS is posi-
tively correlated to latitude . There are a number of case reports showing
remission of MS on gluten-free diets [310–312]. Furthermore, some MS pa-
tients have altered intestinal mucosa [313, 314], suggestive of increased intes-
tinal permeability to dietary antigens. However, MS patients generally do not
show increased antibodies to gliadin , and a number of case studies have
not shown beneﬁcial eﬀects of gluten-free diets [316, 317]. If dietary antigens
containing amino acid sequences similar to putative self antigens, indeed, do
stimulate peripheral T cells, then interventions evaluating the inﬂuence of diet
upon MS would need to consider the potential confounding inﬂuence of
multiple dietary antigens (dairy products, grains, legumes, and yeast) capable
of either molecular mimicry and/or T-cell stimulation.
Psychological and Neurological Illnesses Associated with
Cereal Grain Consumption
Neurological complications have long been recognized in celiac patients
and can include epilepsy, cerebellar ataxias, dementia, degenerative central
nervous system disease, peripheral neuropathies (of axonal or demyelinating
type), and myopathies . A recent study showed that 57% of patients
with neuropathies of unknown cause (25 ataxia, 20 peripheral neuropathy,
5 mononeuritis multiplex, 4 myopathy, 3 motor myopathy, 2 myelopathy)
demonstrated positive titres for antigliadin antibodies, and 16% (40 times
higher than the general population) of this group also had celiac disease
. The cause of neurological dysfunction associated with celiac disease and
antigliadin antibodies is unknown; however it has been suspected that an
immunological mechanism may be involved [315, 318]. Although no clinical
trials have yet been conducted of strict adherence to a gluten-free diet, it has
been suggested that such a diet may result in stabilization or even improvement
of neurological dysfunction .
Epilepsy is observed in 5.5 of 100 cases of celiac disease, and in about
half of these patients bilateral parietooccipital calciﬁcations are found in the
cortical or subcortical areas . This triple association has a common HLA
haplotype and is thought to occur via an underlying immunological disorder
. If gluten-free diets are adopted soon after the onset of epilepsy, seizures
can be severely reduced or eliminated [321, 322].
The behavioral syndrome of autism in children is characterized by few
or no language and imaginative skills, repetitive and self-injurious behavior
and abnormal responses to human and environmental stimuli. The cause of
the syndrome is poorly understood, however it is thought that both genetic
 and immunological factors  may be involved. Autistic children main-
tain HLA haplotypes  that frequently occur in other autoimmune diseases
including rheumatoid arthritis, and they display autoantibodies to myelin
basic protein . Some autistic patients have been shown to have increased
antibodies to gluten and casein ; however, the amelioration of symptoms
in response to gluten-free diets has been equivocal [325, 326].
It has been more than 30 years since Dohan ﬁrst formulated the hypothesis
that opioid peptides found in the enzymatic digests of cereal grain gluten are
a potentiating factor evoking schizophrenia in susceptible genotypes [327,
328]. In a meta-analysis of the more than 50 articles regarding the role of
cereal grains in the etiology of schizophrenia published between 1966 and
1990, Lorenz  concluded: ‘In populations eating little or no wheat, rye
and barley, the prevalence of schizophrenia is quite low and about the same
regardless of type of acculturating inﬂuence.’ In support of this conclusion
are multiple clinical studies [330–332] which have shown that schizophrenic
symptoms improved on gluten-free diets and worsened upon reintroduction.
Furthermore, the incidence of schizophrenia is about 30 times higher in celiac
patients than in the general population , and schizophrenics have elevated
circulating IgA antibodies to gliadin .
There is increasing recognition that in a subset of schizophrenic patients,
autoimmune mechanisms are involved in the etiology of the disease [334,
57Cereal Grains: Humanity’s Double-Edged Sword
335]. Schizophrenics maintain several immunological abnormalities including
increased prevalence of autoimmune disease and antinuclear and other autoan-
tibodies, decreased lymphocyte interleukin-2 (IL-2) production, increased
serum IL-2 receptor concentration, increased serum IL-6 concentrations and
an association with HLA antigens [334, 335]. Similar to other autoimmune
diseases, cereal grains may potentiate their putative autoimmune eﬀects in
schizophrenia via molecular mimicry in which self antigens in brain tissue are
recognized and destroyed by autoaggressive T lymphocytes because of the
structural similarity between brain antigens and foreign dietary antigens. Al-
though this hypothesis may be operative in some schizophrenics, the rapid
remission of symptoms by gluten-free diets, observed in clinical trials [330–332],
is suggestive that an acute mechanism may be additionally responsible, since
it is unlikely that damaged neuronal cells could regenerate in such a short
time frame. In this regard, it has been long recognized that certain gluten
peptides derived from wheat have high opioid-like activity that is naloxone
reversible [336, 337]. The structural identity of these opioid peptides derived
from the enzymatic digest of wheat gluten have recently been characterized and
sequenced [338–340], and there is signiﬁcant evidence utilizing radiolabelled
gliadin isotopes to show that these peptides reach opioid receptors in the brain
and peripheral organs . Thus, it is possible that cereal grains may elicit
behavioral changes via direct interaction with central nervous system opioid
receptors or perhaps via simultaneous immune-mediated reactions against
central nervous system antigens.
From an evolutionary perspective, humanity’s adoption of agriculture,
and hence cereal grain consumption, is a relatively recent phenomenon. Table
3 shows that this event occurred in most parts of the world between 5,500
and 10,000 years ago. Cereal grains represent a biologically novel food for
mankind [341, 342], consequently there is considerable genetic discordance
between this staple food, and the foods to which our species is genetically
Cereal grains lack a number of nutrients which are essential for human
health and well-being; additionally they contain numerous vitamins and min-
erals with low biological availability. Furthermore, the inability of humans to
physiologically overcome cereal grain antinutrients (phytates, alkylresorcinols,
protease inhibitors, lectins, etc.) is indicative of the evolutionary novelty of
this food for our species. This genetic maladaptation between human nutrient
requirements and those nutrients found in cereal grains manifests itself as
vitamin and mineral deﬁciencies and other nutritionally related disorders,
particularly when cereal grains are consumed in excessive quantity. More
disturbing is the ability of cereal grain proteins (protease inhibitors, lectins,
opioids and storage peptides) to interact with and alter human physiology.
These interactions likely occur because of physiological similarities (resultant
from phylogenetic commonalities) shared between humans and many herbi-
vores which have traditionally preyed upon the gramineae family. The second-
ary compounds (antinutrients) occurring in cereal grains (gramineae family),
were shaped by eons of selective pressure and were designed to prevent pre-
dation from traditional predators (insects, birds and ungulates) of this family
of plants. Because primates and hominids evolved in the tropical forest, wherein
dicotyledonous plants prevailed, the human physiology has virtually no evolu-
tionary experience with monocotyledonous cereal grains, and hence very little
adaptive response to a food group which now represents the staple food for
many of the world’s peoples.
Cereal grains obviously can be included in moderate amounts in the diets
of most people without any noticeable, deleterious health eﬀects, and herein
lies their strength. When combined with a variety of both animal- and plant-
based foods, they provide a cheap and plentiful caloric source, capable of
sustaining and promoting human life. The ecologic, energetic eﬃciency
wrought by the widespread cultivation and domestication of cereal grains
allowed for the dramatic expansion of worldwide human populations, which
in turn, ultimately led to humanity’s enormous cultural and technological
accomplishments. The downside of cereal grain consumption is their ability
to disrupt health and well being in virtually all people when consumed in
excessive quantity. This information has only been empirically known since
the discovery of vitamins, minerals and certain antinutrients in the early part
of this century.
The realization that cereal grain peptides interact with and induce change
in human physiology and therefore elicit disease and dysfunction is even newer
and dates to the early 1950s with the discovery of wheat gluten as the causative
agent in celiac disease. In the past 10 years has come the evidence (admittedly
incomplete) that certain cereal peptides may interact with the immune system
to elicit a variety of autoimmune-related diseases. These two seemingly distinct
entities (autoimmune disease and consumption of a staple food) are connected
primarily through an evolutionary collision of dissimilar genes which bear
identical products (molecular mimicry). Although, cereal grain consumption
may appear to be historically remote, it is biologically recent; consequently
the human immune, digestive and endocrine systems have not yet fully adapted
to a food group which provides 56% of humanity’s food energy and 50% of
59Cereal Grains: Humanity’s Double-Edged Sword
Cereal grains are truly humanity’s double-edged sword. Forwithout them,
our species would likely have never evolved the complex cultural and techno-
logical innovations which allowed our departure from the hunter-gatherer
niche. However, because of the dissonance between human evolutionary nutri-
tional requirements and the nutrient content of these domesticated grasses,
many of the world’s people suﬀer disease and dysfunction directly attributable
to the consumption of these foods.
I wish to thank the following individuals for reviewing this manuscript and their
constructive criticisms: Jennie Brand-Miller, S. Boyd Eaton, Staﬀan Lindeberg, Klaus Lorenz,
and Norman Salem. A particular debt of gratitude goes to R. Shatin for his pioneering
thoughts and writings.
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73Cereal Grains: Humanity’s Double-Edged Sword