Characterization of a testicular 17α,20β-dihydroxy-4-pregnen-3-one (a spermiation-inducing steroid in fish) receptor from a teleost, Japanese eel (Anguilla japonica)11The nucleotide sequence data reported in this paper will appear in the DDBJ/EMBL/GenBank nucleotide sequence databases with accession number AB032075.

Laboratory of Reproductive Biology, National Institute for Basic Biology, Okazaki, Japan.
FEBS Letters (Impact Factor: 3.17). 02/2000; 465(1):12-7. DOI: 10.1016/S0014-5793(99)01714-7
Source: PubMed


A cDNA encoding a nuclear 17alpha,20beta-dihydroxy-4-pregnen-3-one (17alpha,20beta-DP, spermiation-inducing hormone in fish) receptor (DPR) was, for the first time, isolated from an eel testis cDNA library. The amino acid sequence of DPR shows high homology with those of human and chicken progesterone receptors. The affinity of the bacterial recombinant DPR ligand binding domain protein for 17alpha,20beta-DP is higher than that of progesterone. In transfection experiments using COS7 cells, the DPR showed progestin-dependent activation of transcription. 17alpha,20beta-DP was the most effective activator of transcription. These results indicate that the cDNA encodes a functional eel DPR, and show that 17alpha,20beta-DP has a nuclear receptor-mediated action in eel testes.

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    • "Phylogenetic analyses of these four proteins clearly indicate that they correspond to the Senegalese sole homologs of StAR, 3b-HSD, 17b-HSD and 20b-HSD. In addition, we identified a partial cDNA for the Senegalese sole PR with high homology to the nuclear receptor for 17,20b-P described in the eel and in other teleost species [11] [41]. "
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    • "But, cortisol does not seem to be an agonist of these receptors (Pinter and Thomas, 1995; Zhu et al., 2003). In male Japanese eel, DOC also displayed a high affinity for a nuclear 17α-20ß-dihydroxyproges- terone (MIS) receptor in the testis and once more, cortisol did not bind or activate it (Todo et al., 2000). That steroid binding profile was also observed in sperm membrane progestogen receptor in Atlantic croaker Micropogonias undulatus (Thomas et al., 2005). "
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    • "ePR1 and ePR2 are coexpressed in the spleen and testis where they are supposed to form heterodimers, cooperating in the regulation of progestin-responsive gene expression (Ikeichi et al., 2002). ePR1 and ePR2 mRNA from Anguilla japonica testis have been cloned and sequenced (Ikeichi et al., 2002; Todo et al., 2000). The derived aa composition showed proteins with an approximate mw of about 100 kDa. "
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