Pathogenicity of Sendai Viruses Adapted into Polarized MDCK Cells

Kagoshima University, Kagosima, Kagoshima, Japan
Journal of Veterinary Medical Science (Impact Factor: 0.78). 01/2000; 61(12):1299-307. DOI: 10.1292/jvms.61.1299
Source: PubMed


Apically and basally released Sendai viruses (SeV) were obtained after infection of polarized Madin-Darby canine kidney (MDCK) cells grown on permeable membrane culture inserts. After 20 passages of adaptation in MDCK cells, we compared their in vivo and in vitro pathogenicity with the parental Mol-strain of SeV. These viruses had comparable in vitro pathogenicity, but the in vivo pathogenicities were varied. The apically released MDCK-adapted virus showed comparable pathogenicity with the parental virus, in contrast with the basally released MDCK-adapted virus, which showed in vivo attenuation.

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Available from: Ryoji Yamaguchi
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    • "Infection of mice with WT Sendai virus induces severe respiratory tract pathology and infection with 1,000 WT pfu can lead to weight loss while infection with 1 million pfu is lethal to mice [20]. In vitro, visible signs of cell stress such as chromatin condensation are observed in cells that are infected with WT Sendai virus and most cells die within 72 hours after infection. "
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