Influence of Flow Patterns on Chromatographic Efficiency in Centrifugal Partition Chromatography

Laboratoire de Biochimie et Molécules Marine, IFREMER, Nantes, France.
Journal of Chromatography A (Impact Factor: 4.17). 03/2000; 869(1-2):339-52. DOI: 10.1016/S0021-9673(99)01184-X
Source: PubMed


Visualization of flow patterns in centrifugal partition chromatography (CPC) was performed with an asynchronous camera and a stroboscope triggered by the CPC rotor, allowing a channel to be selected and observed regardless of rotational speed. Three main types of flow states were noted as a function of rotational speed and flow-rate: jets stuck along channel walls, broken jets and atomization. Our observations emphasize the importance of Coriolis force on flow shape. Chromatographic efficiency was related to the dispersion of the mobile phase in the stationary phase.

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    ABSTRACT: Centrifugal partition chromatography (CPC) is a support-free liquid–liquid separation process that depends for efficiency on the behavior of the two liquid phases. Hydrodynamics of phases was studied according to flow rate and centrifugal acceleration, using a transparent column and a stroboscopic video system. For the heptane-methanol two-phase system, three main flow regimes—stuck film, oscillating sheet, and atomization—are observed, highlighting the coriolis acceleration effect as well as the influence of the column shape. Mass transport in the CPC column is modeled by a plug flow with axial dispersion and mass transfer with a stagnant volume. Model parameters (residence time, Péclet number, partition ratio, and mass-transfer coefficient) are fitted on solute residence-time distribution. Off-column dispersion is an important source of peak broadening in CPC, whereas its irregular geometry provides a plug flow for mobile phase. Importance of flow pattern on mass transfer is demonstrated. CPC interest for preparative applications is confirmed.
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    ABSTRACT: A centrifugal partition chromatography (CPC) is a one-axis centrifugal system operating with two rotary seals. A CPC column is a series of cells linked in a cascade by ducts and regularly disposed around a rotor as cartridges or disks, and submitted to a constant centrifugal acceleration generated by a rotation. This chapter presents the first assumptions on flow patterns. While the rotor of the centrifuge is in motion, the mobile liquid phase is pumped into the cells and “bubbles down” if heavier, or “bubbles up” if lighter, through the stationary phase by the action of the centrifugal force. The “true” flow rate of the bubbles of the mobile phase through the stationary phase is determined by the strength of the applied centrifugal force. As long as the pumping rate (the apparent flow rate) does not exceed the “true” flow rate, it does not affect the resolution of the peaks. This means that the separation can be completed in a short time provided a sufficient centrifugal force is applied.
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