Evaluation of some Moroccan medicinal plant extracts for larvicidal activity. J Ethnopharmacol 73(1-2):293-297
Laboratory of Medicinal Plants and Phytochemistry, Department of Biology, Faculty of Sciences - Semlalia, P.O. Box 2390 40000, Marrakech, Morocco. Journal of Ethnopharmacology
(Impact Factor: 3).
12/2000; 73(1-2):293-7. DOI: 10.1016/S0378-8741(00)00257-9
The larvicidal properties of 16 extracts of four Moroccan medicinal plants: Calotropis procera (Wild.), Cotula cinerea (L.), Solanum sodomaeum (L.) and Solanum elaeagnifolium (CAV.) were tested against Anopheles labranchiae mosquito larvae. Among the extracts tested, nine exhibited high larvicidal activity with LC(50) (24 h) ranging from 28 to 325 ppm.
Available from: Mohamed Izzat Al-Ghannoum
- "Recent studies have demonstrated that insecticidal components of chemicals derived from plants are active against specific target species, biodegradable, non toxic products and potentially suitable for use in integrated management programs (Markouk et al., 2000 and Tare et al., 2004). Nerium oleander L. (Apocynaceae) is an evergreen shrub widely used for ornamental purposes in the Mediterranean region (El- Shazly et al., 1996). "
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ABSTRACT: Different concentrations (25, 30, 35 and 40%) of Nerium oleander L. leaf extracts were tested against the red grain beetle adults Tribolium castaneum (Herbst). Results showed that the percentage mortality ranged from 16.7% in the powder treatment increased to 70% in the alcohol extract at 40% concentration. Highest mortality rate was 49.2% in the alcohol extract, followed by 43.4% in the aqueous extract, while the least (30.9%) was recorded for the dry powder of N. oleander leaves. The results also showed an attraction and repellent effects of the extracts on the pest adults and there was a positive correlation between high mortality rate of the tested products and repellent effects on the adults, while a negative correlation was recorded between mortality rate and attractiveness.
Available from: Dr. Jayapal Subramaniam
- "Hence, new safe alternatives botanical products are useful and desirable tools in most pest management programs because they are effective and often non toxic to natural enemies having low environmental impact (Schmutterer, 1995; Haseeb et al., 2004; Xu et al., 2004). Botanicals have been shown to be effective larvicides and adulticides and in some cases are more eco-friendly against non-target animals and have been successfully tested for various biocontrol programmes (Markouk et al., 2000). "
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ABSTRACT: The present study explored the effects of Andrographis lineate (Acanthaceae), Cassia auriculata (Cesalpinaceae) and Solanum nigrum (Solanaceae) tested against third instar larvae of diamondback moth, Plutella xylostella. The medicinal plants were collected from Maruthamalai Hills, near the Bharathiar University campus in Coimbatore, Tamil Nadu, India. The plant extracts (chloroform, acetone and methanol) showed larvicidal effects after 48 h of exposure. Among three plants tested, the highest larvicidal activity was observed in methanol extract of A. lineate followed by C. auriculata and S. nigrum against P. xylostella. The rates of mortality were directly proportional to concentration of extract. The chloroform, acetone and methanol extract of A. lineate with LC 50 values of 3.724, 2.424 and 2.190 per cent; C. auriculata with LC 50 values of 4.238, 3.198 and 2.693 per cent; S. nigrum with LC 50 values of 4.921, 3.573 and 3.217 per cent respectively, against third instar larvae of P.xylostella. The Chi-square values are significant at p<0.05 level. The Chi-square values in the bioassays indicated probably the heterogeneity of the test population. The 95 per cent confidence limits LC 50 (LCL–UCL) and LC 90 (LCL–UCL) were also calculated. No mortality
Available from: Emma Arvelo
- "Extractos de la planta en solventes orgánicos, como etanol y acetona, han presentado actividades insecticida (Moursy, 1997), larvicida (Markouk et al., 2000), antibacteriana y antiparasitaria (Larhsini et al., 1999). Una fracción de proteínas obtenida del extracto acuoso del tallo inhibió el crecimiento in vitro de Staphylococcus aureus y Enterobacter aerogenes a una concentración de 25 µg/mL con una eficiencia comparable a la del cloramfenicol utilizado como control positivo (Samy y Chow, 2012). "
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ABSTRACT: y del Caribe de Plantas Medicinales y Aromáticas 12 (5): 476 -492 ISSN 0717 7917 www.blacpma.usach.cl Artículo Original | Original Article 476 Citotoxicidad in vitro de extractos laticiferos de Calotropis procera (Aiton) W.T. Aiton y Pedilanthus tithymaloides (L.) Poit. [In vitro cytotoxicity of laticifers extracts from Calotropis procera (Aiton) W.T and Pedilanthus tithymaloides (L.) Poit.] Abstract In order to assess the anticancer action of extracts obtained by latex from Calotropis procera and Pedilanthus tithymaloides, samples were collected from adult plants. Soluble proteins were extracted with 16 µL of 50 mM sodium acetate pH 5/μg integral latex and centrifugation at 16,000 x g for 15 min, the supernatant was named "latex crude extract" (LCE). The "latex methanolic extract" (LME) was obtained on dried latex. Both extracts were tested in vitro by cytotoxic and cytostatic activity in Jurkat cell cultures. Cellular viability, proliferation, necrosis and apoptosis were evaluated. LCE and LME of C. procera were found with cytotoxic and cytostatic activity after 24 incubation hours (p < 0,05) with doses from 1µg/mL. The LCE and LME of P. tithymaloides presented cytotoxic effect (p < 0,05) from 50 µg/mL and from 1µg/mL, respectively. Resumen Con el objetivo de evaluar el potencial anticanceroso de extractos de látex de Calotropis procera y Pedilanthus tithymaloides se colectaron muestras de plantas adultas. Las proteínas solubles fueron extraídas con 16 µL de acetato de sodio 50 mM pH 5/µg de látex integral y centrifugación a 16.000 x g durante 15 min, denominándose al sobrenadante "extracto crudo de látex" (ECL). El "extracto metanólico de látex" (EML) se obtuvo sobre látex deshidratado. Ambos extractos fueron probados en su actividad citotóxica y citostática in vitro sobre cultivos de células Jurkat. Se realizaron estudios de viabilidad, proliferación, necrosis y apoptosis celular. El ECL y el EML de C. procera presentaron actividad citotóxica y citostática después de 24 y 48 horas de incubación (p < 0,05) con dosis desde 1 µg/mL. Los ECL y EML de P. tithymaloides presentaron efectos citotóxicos (p < 0,05) a partir de 50 µg/mL y desde 1 µg/mL respectivamente. Palabras Clave: Látex, cultivos celulares in vitro, células Jurkat, citotóxico, citostático.
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