Prevalance of American trypanosomiasis (Chagas disease) among dogs in Oklahoma

Oklahoma State University - Stillwater, SWO, Oklahoma, United States
Journal of the American Veterinary Medical Association (Impact Factor: 1.56). 01/2001; 217(12):1853-7. DOI: 10.2460/javma.2000.217.1853
Source: PubMed


To determine the prevalence of Trypanosoma cruzi infection among dogs in Oklahoma.
Cross-sectional study.
301 owned or impounded dogs related by ownership or general geographic location to 3 dogs determined to have trypanosomiasis.
Blood samples were obtained from dogs between November 1996 and September 1997. Infection status was determined by use of a radioimmunoprecipitation assay. Second blood samples were obtained from some of the seropositive dogs for study by hemoculture and polymerase chain reaction (PCR) assay. Sites where infected dogs were found were inspected for triatomine insects, and light traps were used for vector trapping.
11(3.6%) dogs were seropositive for T. cruzi infection. Ten of the 11 were owned rural hunting dogs. Protozoal organisms isolated from the blood of 1 seropositive dog were identified as T. cruzi by PCR testing. Only 1 adult Triatoma sanguisuga was captured in a light trap at a site near infected dogs; this insect was not infected.
Our findings suggest that T. cruzi is enzootic in eastern Oklahoma. Measures that would reduce the risk of dogs acquiring T. cruzi infection are unlikely to be acceptable to their owners, and no effective drugs are available for treatment. The presence of T. cruzi-infected dogs poses a threat of transmission to persons at risk of exposure to contaminated blood Veterinarians who practice in the southern United States should be cognizant of this blood borne zoonosis and educate all personnel about appropriate precautions.

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    • "Many of these mammals are peri-urban species that adapt well to human-modified landscapes and, if infected, can bring T. cruzi closer to humans and their canine companions. In turn, when triatomines are present in the local environment, there may be a subsequent increased risk ofvectorial T. cruzi transmission to people, and an even greater transmission risk to dogs, who likely acquire T. cruzi via ingestion of infected vectors[10,11]. In 2006, the Texas Veterinary Medical Diagnostic Laboratory reported 18.6% of 532 dogs presumably clinically ill with cardiac disease to be seropositive for T. cruzi[12]. In addition, canine serological surveys in states such as Tennessee, Louisiana and Texas indicate that T. cruzi infection is not an uncommon occurrence, even in apparently healthy domestic dogs131415. "
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    ABSTRACT: Trypanosoma cruzi, causative agent of Chagas disease in humans and dogs, is a vector-borne zoonotic protozoan parasite that can cause fatal cardiac disease. While recognized as the most economically important parasitic infection in Latin America, the incidence of Chagas disease in the United States of America (US) may be underreported and even increasing. The extensive genetic diversity of T. cruzi in Latin America is well-documented and likely influences disease progression, severity and treatment efficacy; however, little is known regarding T. cruzi strains endemic to the US. It is therefore important to expand our knowledge on US T. cruzi strains, to improve upon the recognition of and response to locally acquired infections.
    Full-text · Article · Jan 2016 · PLoS Neglected Tropical Diseases
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    • "Burkholder et al. (1980) found that 12% of 136 dogs Texas were positive, while Shadomy et al. (2004) found that 15% of 356 dogs from Texas were positive. Bradley et al. (2000) demonstrated that 4% of 301 dogs from Oklahoma were positive. Nieto et al. (2009) found that 22% of 50 dogs from Louisiana were positive. "
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    ABSTRACT: Trypanosoma cruzi was demonstrated in blood smears and heart tissue from a 5-year old, female, English Cocker Spaniel that had never been outside of the state of Virginia, USA. Plasma from the dog was positive in a commercially available immunochromatographic dipstick assay for T. cruzi and negative in an immunochromatographic dipstick assay for visceral Leishmania spp. The plasma from the dog had an indirect immunofluorescent antibody titer of 1:800 against epimastigotes of T. cruzi while the titer was 1:50 against promastigotes of L. infantum. The parasite was isolated from the blood in vitro from the dog (TcVT-1 isolate) and used to experimentally infect female C3H and ICR mice. The parasite was nonpathogenic for experimentally inoculated mice. DNA was isolated from parasites grown in vitro and used to determine that the genotype of T. cruzi present in the dog was genotype TcIV. This genotype is common in raccoons, Procyon lotor, in North America and suggests that raccoons may serve as reservoirs for canine infection.
    Full-text · Article · Jan 2012 · Veterinary Parasitology
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    • "The estimated number of infected persons living in the United States is 300,000 or more, based on estimated disease rates by country of origin [3]. Furthermore, the parasite can be found in reduviid bugs and mammals in the southern regions of the United States [4] [5] [6], and there have been a few reported cases of autochthonous, and transplant-and blood donation-related transmissions in humans [7] [8] [9]. "
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    ABSTRACT: Chagas disease affects 8-11 million people, mostly in Latin America. Sequelae include cardiac, peripheral nervous and/or gastrointestinal disorders, thus placing a large economic and social burden on endemic countries. The pathogenesis and the evolutive pattern of the disease are not fully clarified. Moreover, available drugs are partially effective and toxic, and there is no vaccine. Therefore, there is an urgent need to speed up basic and translational research in the field. Here, we applied automated high-content imaging to generate multiparametric data on a cell-by-cell basis to precisely and quickly determine several parameters associated with in vitro infection of host cell by Trypanosoma cruzi, the causative agent of Chagas disease. Automated and manual quantifications were used to determine the percentage of T. cruzi-infected cells in a 96-well microplate format and the data generated was statistically evaluated. Most importantly, this automated approach can be widely applied for discovery of potential drugs as well as molecular pathway elucidation not only in T. cruzi but also in other human intracellular pathogens.
    Full-text · Article · Dec 2010 · Parasitology International
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