Therapeutic potential of curcumin in human prostate cancer. III. Curcumin inhibits proliferation, induces apoptosis, and inhibits angiogenesis of LNCaP prostate cancer cells

Molecular Urology Laboratory, Department of Urology, College of Physicians and Surgeons, Columbia University, New York, New York, USA.
The Prostate (Impact Factor: 3.57). 06/2001; 47(4):293-303. DOI: 10.1002/pros.1074
Source: PubMed


Earlier work from our laboratory highlighted the therapeutic potential of curcumin (turmeric), used as a dietary ingredient and as a natural anti-inflammatory agent in India and other Southeast Asian countries. This agent was shown to decrease the proliferative potential and induce the apoptosis potential of both androgen-dependent and androgen-independent prostate cancer cells in vitro, largely by modulating the apoptosis suppressor proteins and by interfering with the growth factor receptor signaling pathways as exemplified by the EGF-receptor. To extend these observations made in vitro and to study the efficacy of this potential anti-cancer agent in vivo, the growth of LNCaP cells as heterotopically implanted tumors in nude mice was followed.
The androgen-dependent LNCaP prostate cancer cells were grown, mixed with Matrigel and injected subcutaneously into nude mice. Experimental group received a synthetic diet containing 2% curcumin for up to 6 weeks. At the end point, sections taken from the excised tumors were evaluated for pathology, cell proliferation, apoptosis, and vascularity.
Curcumin causes a marked decrease in the extent of cell proliferation as measured by the BrdU incorporation assay and a significant increase in the extent of apoptosis as measured by an in situ cell death assay. Moreover, a significant decrease in the microvessel density as measured by the CD31 antigen staining was also seen.
Curcumin could be a potentially therapeutic anti-cancer agent, as it significantly inhibits prostate cancer growth, as exemplified by LNCaP in vivo, and has the potential to prevent the progression of this cancer to its hormone refractory state.

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    • "There was an irreversible inhibition of CD13/aminopeptidase N (APN) caused by curcumin " s binding ability to APN (Shim et al, 2003)). Several investigators (Arbiser et al, 1998; Dorai et al, 2001) reported in their in vivo studies that curcumin inhibits proliferation and angiogenesis of LNCaP prostate cancer cells. cDNA microarray analysis showed that curcumin inhibits cell cycle progression of endothelial cells by upregulating cyclin-depenent kinase inhibitor (Park et al, 2002). "
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    ABSTRACT: Numerous treatment modalities are available for variety of systemic and dental diseases; however, the main drawback of conventional medicinal therapies is its various side effects causing harm to the patient. This diverted the interest of researchers towards an alternative approach where natural compounds derived from plants could be used for treating those patients. Curcumin is such an alternative which exhibits a number of medicinal properties and has been used from the centuries. This article discusses the efficacy of curcumin in maintenance of oral health, in particular, and overall health, in general. Curcumin (diferuloylmethane) is a polyphenol derived from the curcuma longa plant, commonly known as turmeric, is a herb known for its medicinal properties. It has a variety of therapeutic properties like anti-inflammatory, anti-oxidant, anti-microbial, hepatoprotective, immunostimulant, antiseptic , anti-angiogenic, apoptotic and anti-mutagenic. All these beneficial properties makes this compound quite more useful in dental field especially in treating periodontal diseases and cancers involving head and neck region and oral cavity. It can also be formulated as a pit and fissure sealant, mouth wash, and subgingival irrigant in different preparations in different dosages and also as a component in local drug delivery system in gel form. INTRODUCTION Most of the anti-cancer drugs are very toxic, highly inefficient for cancer treatment or highly expensive. Thus, these drugs show their limited potential in cancer therapy and beyond the reach of the majority of affected individuals. Hence, an alternative medicinal product without exhibiting such drawbacks will be good option as an anti-cancer drug. Natural phytochemicals isolated from plants used as traditional medicines are referred as such sources. Curcumin, derived from the dried rhizome of the East Indian turmeric plant (Curcuma longa), a perennial herb belonging to the Zingiberacae (ginger) family of botanicals, has drawn attention as alternative source in cancer therapy. This turmeric plant is 3 feet in height and Corresponding Author
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    • "Curcumin has shown an apoptotic effect by inhibiting various genes such as protein tyrosine kinase, protein kinase C, c-myc mRNA expression, and bcl-2 mRNA expression [43] and also mitochondrial pathway. Earlier studies have shown that curcumin possesses an apoptotic activity in different types of cancer cell such as human colon cancer cells, stomach, and skin tumors, breast cancer cells, and prostate cancer cells [44–47]. Study of colon cancer cell line showed that apoptosis was increased in response to curcumin [48, 49]. "
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    • "Curcumin is also capable of suppressing the activity of several common mutagens and carcinogens in a variety of cell types in both in vitro and in vivo studies. The anti carcinogenic effects of it is due to direct antioxidant and free-radical scavenging effects, as well as its ability to indirectly increase glutathione levels, hepatic detoxification of mutagens and carcinogens, and inhibiting nitrosamine formation (Limtrakul et al., 1997; Hanif et al., 1997; Dorai et al., 2001; Mehta and Moon, 1991; Soudamini and Kuttan, 1989; Azuine and Bhide, 1992; Pizorrno and Murray, 1999). Turmeric has been widely used for the treatment of some diseases. "

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