Prevalence of Neospora caninum Infection in Dogs From Beef-Cattle Farms, Dairy Farms, and From Urban Areas of Argentina

National University of La Plata, Eva Perón, Buenos Aires, Argentina
Journal of Parasitology (Impact Factor: 1.23). 08/2001; 87(4):906-7. DOI: 10.1645/0022-3395(2001)087[0906:PONCII]2.0.CO;2
Source: PubMed


Prevalence of anti-Neospora caninum antibodies was determined in sera of 320 dogs from Argentina using the indirect fluorescent antibody test (IFAT). Antibodies to N. caninum were found in 121 of 320 (37.8%) sera with titers of 1:50 (21 dogs), 1:100 (23 dogs), 1:200 (23 dogs), 1:400 (17 dogs), 1:800 (23 dogs), and > or = 1:1.600 (14 dogs). The seropositivity (IFAT, > or = 1:50) was higher in dogs from dairy (48% of 125) and beef (54.2% of 35) farms than in dogs from urban areas (26.2% of 160). Prevalence of anti-N. caninum antibodies was higher in dogs more than 12 mo of age (47.7%, 105 of 222) versus in 12-mo-old or younger dogs (12.7% of 86), suggesting postnatal exposure of N. caninum infection in dogs.

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Available from: Carlos Manuel Campero, Mar 02, 2015
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    • "Thus, identification of seropositive animals and seroprevalence data in dog populations is extremely important for control and prevention strategies. In South America, there are variations in the prevalence of neosporosis in the canine population, for example: 20% in Uruguay (BARBER et al., 1997), 37.8% in Argentina (BASSO et al., 2001) and 18% in Chile (PATITUCCI et al., 2001). In Brazil, the prevalence is between 8.4%, which was found in the state of Paraiba state (AZEVEDO et al., 2005), and 15.6% in the state of Rio Grande do Sul (CUNHA et al., 2008). "
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    ABSTRACT: Neosporosis is a disease caused by the protozoon Neospora caninum that leads to significant economic losses in many countries. In the present study, we report on use of the recombinant protein NcSRS2 of N. caninum expressed in Pichia pastoris in an indirect immunoenzymatic assay (ELISA) for diagnosing neosporosis infection in sheep and dogs. We observed that the ELISA test yielded specificity of 94.5% and sensitivity of 100% for sheep and specificity of 93.3% and sensitivity of 100% for dogs. We observed that the sensitivity was higher than shown by the indirect fluorescent antibody test, and this was confirmed by means of Western blot. The results from this study suggest that the recombinant protein expressed in P. pastoris is a suitable antigen for use in immunodiagnosis to detect N. caninum in two important species exposed to this parasitosis.
    Preview · Article · Jun 2015 · Revista brasileira de parasitologia veterinaria = Brazilian journal of veterinary parasitology: Orgao Oficial do Colegio Brasileiro de Parasitologia Veterinaria
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    • "Detection of Neospora sp. antibodies in cart horses 69 definitive hosts (McALLISTER et al., 1998; LINDSAY et al., 1999; BASSO et al., 2001; WAPENAAR et al., 2006; KING et al., 2010). Horses can serve as an intermediate host. "
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    ABSTRACT: Neospora caninum is a protozoan parasite which affects dogs as definitive hosts and several mammalian species as intermediate hosts mainly causing abortions and central nervous system disorders. The reemerging population of cart horses for carrying recycling material in urban areas of major cities in Brazil may have an impact on disease spreading, and these animals may be used as sentinels for environmental surveillance. Thus, the present study investigated the frequency of Neospora sp. antibodies in cart horses from Curitiba and surrounding areas, Paraná State, Southern Brazil. IgG antibodies against Neospora sp. were detected using indirect fluorescence antibody test (IFAT), and titers equal to or higher than 1:50 were considered reactive. Of all samples, 14/97 (14.4%) were positive: 2/29 (6.9%) were younger than 5; 5/26 (19.2%) between 6 and 9; and 6/31 (19.4%) older than 10 years of age. One of the 11 animals with unknown age was positive (9.1%). Cart horses are likely to be more exposed to dog feces and to Neospora sp. oocyst contamination in urban settings and a lower frequency of disease in dogs may have a negative impact on horse infection risk in these areas.
    Full-text · Article · Mar 2012 · Revista brasileira de parasitologia veterinaria = Brazilian journal of veterinary parasitology: Orgao Oficial do Colegio Brasileiro de Parasitologia Veterinaria
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    • "Similar investigations in other parts of the world have also shown different rates of infection: antibodies to N. caninum were detected in 121 out of 320 (37.8%) dogs in Argentina (Basso et al., 2001); 22% of 200 dogs from New Zealand (Reichel, 1998); 10% of 150 dogs from Turkey (Coskun et al., 2000); 6.7% of 163 dogs from Brazil (Mineo et al., 2001), 10% of 500 pet dogs, and 25% of 611 stray dogs from Brazil (Gennari et al., 2002); and 6.4% of 1,058 dogs from Italy (Cringoli et al., 2002). The high prevalence of infection in Iran indicates the necessity of control strategies to be performed in dogs and cattle populations. "
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    ABSTRACT: may not store these pages in any form except for your own personal use. All other usage or distribution is illegal under international copyright treaties. Permission to use any of these pages in any other way besides the before mentioned must be gained in writing from the publisher. This article is exclusively copyrighted in its entirety toOJVR.This article may be copied once but may not be, reproduced or re-transmitted without the express permission of the editors. This journal satisfies the refereeing requirements (DEST) for the Higher Education Research Data Collection (Australia). Linking:To link to this page or any pages linking to this page you must link directly to this page only here rather than put up your own page. ABSTRACT Khordadmehr M, Hosseini SMH. Mohsenifar E, Namavari MM, Khordadmehr S., Seroprevalence of Neospora caninum in farm and household dogs determined by ELISA, Online J Vet Res., 16 (4):172-181, 2012. Neospora caninum is an important cause of abortion in dairy cattle worldwide. The dog is the definitive host for N.caninum which also infects dairy cattle. We report seroevalence of Neospora caninum infection in dogs in Fars province in Iran. Serum samples (180) were collected from dogs of different sex, age, and breed and subjected to a modified agglutination (MAT) and standardized dot-ELISA test. Fifty nine samples (54.62%) were positive for N. caninum antibody by dot-ELISA and 49 samples (44.44%) were positive by MAT in titers of 1:100 and 1:80, respectively. There were no significant differences in serological values between different groups of age, sex, household or farm dogs. There was 79.8% agreement between dot-ELISA and MAT tests.
    Full-text · Article · Jan 2012
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