Multiplication of Musa from excised stem tips
Department of Biochemistry, Division of Biological Sciences, State University of New York at Stony Brook 11794, USA. Annals of Botany
(Impact Factor: 3.65).
Rapidly multiplying cultures were established from excised shoot tips of two dessert banana clones ('Philippine Lacatan' and 'Grande Naine') and two plantain clones ('Pelipita' and 'Saba'). Apices cultured on semi-solid media produced single shoots while apices placed in liquid media produced shoot clusters. Individual shoots were induced to form multiple shoot clusters by longitudinally splitting the shoot through the apex. Shoot multiplication was stimulated maximally by 5 mg l-1 benzylaminopurine. Rooted plants were produced by treating shoots with auxins. Growth rates based on increase in f. wt of the four clones were compared. During a 4-week culture period 'Pelipita' showed a fivefold increase in f. wt while 'Grande Naine', 'Philippine Lacatan' and 'Saba' showed increases exceeding tenfold.
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- "Generally, cytokinin helps in shoot multiplication and auxin helps in root formation of multiplied shoots. Commonly, the requirement of cytokinin and auxin depends on the variety of banana and culture conditions (Cronauer and Krikorian, 1984). Therefore, the present investigation was undertaken with the following objectives: i) to determine the suitable concentrations of BAP and NAA on in vitro regeneration of banana cultivars, ii) to allow for the rapid multiplication of uniform and high quality clonal planting materials and iii)to supply planting material in large quantities especially for large-scale cultivation. "
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- "(Madhulatha et al., 2004; Venkatachalam et al., 2007; Bairu et al., 2008; Jafari et al., 2011). Other studies have reported the use of isopentyladenine (2-ip) (Dore et al., 2008), zeatin (Vuylsteke and Langhe, 1985) and kinetin (Cronaeur and Krikorian, 1984). However, the use of diphenyl urea derivatives (such as TDZ) is rare, except for a few reports (Youmbi et al., 2006; Shirani et al., Kahia et al. 41 2009; Bohra et al., 2014). "
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- "Microbes like bacteria, fungi and virus are the most common contaminants observed in cultures. To overcome this sterilizing agents with different concentrations are generally used like 0.25% sodium hypochlorite (Vessey and Revera, 1981) chlorine saturated water for 15-20 minutes (Dore et al., 1983), 0.05% sodium hypochlorite (Cronauer and Krikarion, 1984a). "
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ABSTRACT: ASTRACT The study was undertaken with a view to establish a protocol for in vitro plant regeneration from shoot tip explants of banana. Different concentrations of BAP (0.0, 1.0, 2.0, 3.0, 4.0, 5.0, 6.0 and 7.0 mg/l) and IBA (0.0, 1.0, 2.0, 3.0, 4.0 and 5.0 mg/l) was used in MS medium to assess the influence on in vitro shoot regeneration and subsequent root formation of Amritasagar and Sabri banana cultivars. Maximum single shoot formation (50.0% and 30.0%), number of single shoot (3.50 and 2.00 in), longest shoot (2.64 cm and 2.16 cm) were found from 0.5 mg/l BAP while maximum number of roots (3.83 and 2.50) and longest root (3.60 cm and 3.10 cm) was found from 0.3 mg/l IBA in Amritasagar and Sabri respectively. The survival of the plantlets of both cultivars was more than 82% under ex vitro condition. 0.5 mg/l BAP and 0.3 mg/l IBA can be used with MS media for shoot and root formation of Amritasagar and Sabri banana cultivars through shoot tip culture.
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