The Application of Three-Dimensional Internal Structure Microscopy in the Observation of Mare Ovary
College of Bioresource Sciences, Nihon University, Kanagawa, Japan. Anantomia Histologia Embryologia
(Impact Factor: 0.67).
11/2001; 30(5):309-12. DOI: 10.1046/j.1439-0264.2001.00335.x
The ovary of the mare has a unique structure which differs totally from that of other mammals. However, because of its relatively large size, conventional histological techniques were unsuitable for the observation of the internal structure of the whole ovary. Three-dimensional internal structure microscopy (3D-ISM) consists of a cryotome-CCD camera-laser disc recorder-PC-based control system coupled with a graphic workstation. The internal structure of the ovary is observed by processing over more than 1,000 stored images of serially sliced surfaces of each frozen equine ovary. The 3D reconstruction was done using the full-coloured, volume-rendering method. The relationship between the localization of medulla, cortex and ovulation fossa was clarified. The ovulation fossa is localized in the centre of the ovary and is surrounded by a broad ovarian cortex. A trace of ovulation was observed only at the ovulation fossa. Medulla are localized in narrow peripheral areas. The phenomenon of the competition to occupy the cortical area ahead of the ovulation fossa by developing secondary follicles was visualized. Spatial localization of various sized follicles was identified from 3D-reconstructed images. In this study, it has been clarified that application or this novel computerized technique can clarify the anatomical arrangements of the equine ovary and the complex mechanism of equine follicular development.
Available from: Hideo Yokota
- "Htt kept twelve kinds of UREs registered in TRANSFAC, and among them the UREs for Gata, Pax and Aml1 appeared in the ranges of more than a half of these genes (Fig. 2). Next, we employed our original device, 3D-ISM system for consistent data production of gene expression contributing to the precise mathematical profiling analysis and the discovery of gene clusters with similar expression patterns   . The extracted RNA from 61 frozen-section samples obtained from the six mice brains sliced in different directions with 3D-ISM was analyzed with microarray, and the gene expression profile was clustered (Fig. 3). "
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ABSTRACT: Inactivation of serotonin transporter (HTT) by pharmacologically in the neonate or genetically increases risk for depression in adulthood, whereas pharmacological inhibition of HTT ameliorates symptoms in depressed patients. The differing role of HTT function during early development and in adult brain plasticity in causing or reversing depression remains an unexplained paradox. To address this we profiled the gene expression of adult Htt knockout (Htt KO) mice and HTT inhibitor-treated mice. Inverted profile changes between the two experimental conditions were seen in 30 genes. Consistent results of the upstream regulatory element search and the co-localization search of these genes indicated that the regulation may be executed by Pax5, Pax7 and Gata3, known to be involved in the survival, proliferation, and migration of serotonergic neurons in the developing brain, and these factors are supposed to keep functioning to regulate downstream genes related to serotonin system in the adult brain.
Available from: Shigeaki Ueno
- "Do et al.  measured the surface area and volume of a sample of broccoli with the MSIPS. Yokota et al.   and Ogawa et al.  applied 3-D internal structure microscopy to observe the internal structure of several histological biomaterials and vegetables. "
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ABSTRACT: A Micro-Slicer Image Processing System (MSIPS) has been applied to observe the ice crystal structures formed in frozen dilute solutions. Several characteristic parameters were also proposed to investigate the three-dimensional (3-D) morphology and distribution of ice crystals, based on their reconstructed images obtained by multi-slicing a frozen sample with the thickness of 5 μm. The values of characteristic parameters were determined for the sample images with the dimension of 530×700×1000 μm. The 3-D morphology of ice crystals was found to be a bundle of continuous or dendrite columns at any freezing condition. The equivalent diameter of ice crystals were in the range of 73–169 μm, and decreased exponentially with increasing freezing rate at the copper cooling plate temperature of −20 to −80 °C. At the Tcp −40 °C, the volumes of ice crystals were in the range of 4.6×104 μm3 to 3.3×107 μm3, and 36 ice columns were counted in the 3-D image.
Available from: Higuchi Toshiro
- "TheMSIPS    iscomposedofamulti-slicingsectiontoexposethecross-sectionsofasample,an observationsectionandanimagingsectionasshowninFig.1.Thecross-sectionalimagesofexposedsurfacesafter slicingwerecaptureddirectlywithaCCDcamera(DX930,Sony,Japan)throughafluorescentmicroscope (BX-FLA,Olympus,Japan),andrecordedintoalaservideodisc(LVR-300AN,Sony,Japan).Themulti-slicing sectionwasmaintainedat-40@BULLETŽwithanimmersioncoolerduringobservation . "
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ABSTRACT: A Micro-Slicer Image Processing System (MSIPS) has been applied to observe the ice crystal structures formed in frozen dilute solutions. Several characteristic parameters were also proposed to investigate the three-dimensional (3-D) morphology and distribution of ice crystals, based on their reconstructed images obtained by multi-slicing a frozen sample with the thickness of 5µm. The values of characteristic parameters were determined for the sample images with the dimension of 530 x 700 x 1000µm. The 3-D morphology of ice crystals was found to be a bundle of continuous or dendrite columns at any freezing condition. The equivalent diameter of ice crystals were in the range of 73µm to 169µm. At a temperature of a copper cooling plate of -40℃, the volumes of ice crystals were in the range of 4.6 x 10 4 µm 3 to 3.3 x 10 7 µm 3 , and 36 ice columns were counted in the 3-D
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