Primary Role for CD4+ T Lymphocytes in Recovery from Oropharyngeal Candidiasis

Oral Biology and Pathology, School of Dentistry, University of Queensland, Brisbane, Queensland, Australia.
Infection and Immunity (Impact Factor: 3.73). 03/2002; 70(2):724-31. DOI: 10.1128/IAI.70.2.724-731.2002
Source: PubMed


Oropharyngeal candidiasis is associated with defects in cell-mediated immunity and is commonly seen in human immunodeficiency
virus positive individuals and AIDS patients. A model for oral candidiasis in T-cell-deficient BALB/c and CBA/CaH nu/nu mice was established. After inoculation with 108 Candida albicans yeasts, these mice displayed increased levels of oral colonization compared to euthymic control mice and developed a chronic
oropharyngeal infection. Histopathological examination of nu/nu oral tissues revealed extensive hyphae penetrating the epithelium, with polymorphonuclear leukocyte microabscess formation.
Adoptive transfer of either naive or immune lymphocytes into immunodeficient mice resulted in the recovery of these animals
from the oral infection. Reconstitution of immunodeficient mice with naive CD4+ but not CD8+ T cells significantly decreased oral colonization compared to controls. Interleukin-12 and gamma interferon were detected
in the draining lymph nodes of immunodeficient mice following reconstitution with naive lymphocytes. This study demonstrates
the direct requirement for T lymphocytes in recovery from oral candidiasis and suggests that this is associated with the production
of cytokines by CD4+ T helper cells.

Download full-text


Available from: Gregory J Seymour
  • Source
    • "In agreement with these findings is the marked susceptibility of HIV-infected subjects to oral candidiasis when CD4+ T cells are depleted, suggesting a role for both Th1 and Th17 cell functional CD4 subsets. In addition, depletion of IL-12 resulted in acute susceptibility to oral infection in a mouse experimental model (Farah et al., 2002; Fidel, 2006; Zakikhany et al., 2007). In a report by Conti et al. (2009) susceptibility to oropharyngeal candidiasis (OPC) in mice with impaired Th1 and/or Th17 responses was compared. "
    [Show abstract] [Hide abstract]
    ABSTRACT: The commensal fungus Candida albicans causes mucosal candidiasis in the rapidly expanding number of immunocompromised patients. Mucosal candidiasis includes oropharyngeal, esophageal, gastrointestinal, and vaginal infections. Vulvovaginal candidiasis (VVC) and antimycotic-refractory recurrent VVC is a frequent problem in healthy childbearing women. Both these mucosal infections can affect the quality of life and finding new therapeutical and preventive approaches is a challenge. A vaccine against candidal infections would be a new important tool to prevent and/or cure mucosal candidiasis and would be of benefit to many patients. Several Candida antigens have been proposed as vaccine candidates including cell wall components and virulence factors. Here we discuss the recent progress and problems associated with vaccination against mucosal candidiasis.
    Full-text · Article · Aug 2012 · Frontiers in Microbiology
  • Source
    • "Establishment of infection at mucosal sites generally requires treatment with immunosuppressive agents, oestrogen, or antibiotics prior to infection, or the use of germ-free animals [66] [67] [68]. However, the nude (Foxn1 nu ) mouse model of oral infection allows infection to be established without any immunosuppression or other pretreatment [69]. Greater detail can be found in more extensive reviews of these infection models [67] [68] [70] [71]. "
    [Show abstract] [Hide abstract]
    ABSTRACT: Although normally commensals in humans, Candida albicans, Candida tropicalis, Candida parapsilosis, Candida glabrata, and Candida krusei are capable of causing opportunistic infections in individuals with altered physiological and/or immunological responses. These fungal species are linked with a variety of infections, including oral, vaginal, gastrointestinal, and systemic infections, with C. albicans the major cause of infection. To assess the ability of different Candida species and strains to cause infection and disease requires the use of experimental infection models. This paper discusses the mucosal and systemic models of infection available to assay Candida virulence and gives examples of some of the knowledge that has been gained to date from these models.
    Full-text · Article · Jan 2012 · International Journal of Microbiology
  • Source
    • "In contrast to their resistance to systemic infection, T-cell-deficient (nude) mice are acutely susceptible to oral infection [53]. Furthermore, mice in which the T-cell receptor δ- and α-chains had been genetically deleted were also highly susceptible to orogastric candidiasis [18] but remained resistant to acute systemic candidiasis. "
    [Show abstract] [Hide abstract]
    ABSTRACT: IL-12 is a cytokine with links to both innate and adaptive immunity systems. In mice, its deletion leads to acute susceptibility to oral infection with the yeast Candida albicans, whereas such mice are resistant to systemic disease. However, it is an essential component of the adaptive response that leads to the generation of Th1-type cytokine responses and protection against disseminated disease. This paper presents an overview of the role of IL-12 in models of systemic and mucosal infection and the possible relationships between them.
    Full-text · Article · Jan 2011 · Clinical and Developmental Immunology
Show more