Determination of Difloxacin and Sarafloxacin in Chicken Muscle Using Solid-Phase Extraction and Capillary Electrophoresis

ArticleinJournal of Chromatography B 767(2):313-9 · March 2002with8 Reads
Impact Factor: 2.73 · DOI: 10.1016/S1570-0232(01)00587-6 · Source: PubMed

    Abstract

    This paper describes a method for residue analysis of difloxacin and sarafloxacin in chicken muscle. Clean-up and preconcentration of the samples are effected by solid-phase extraction (C18) and the determination is carried out by capillary electrophoresis using a photodiode array detection system. The method was validated with satisfying results. The calibration graphs are linear for difloxacin and sarafloxacin from 50 to 300 microg/kg. The limit of detection obtained for difloxacin and sarafloxacin are 10 and 25 microg/kg, respectively, which allows the detection of positive muscle samples at the required maximum residue limits of European Union.