Plasma steroid concentrations in relation to size and age in juvenile alligators from two Florida lakes

Article (PDF Available)inComparative Biochemistry and Physiology - Part A Molecular & Integrative Physiology 131(4):923-30 · May 2002with46 Reads
DOI: 10.1016/S1095-6433(02)00025-9 · Source: PubMed
Abstract
Previous studies have reported a number of physiological differences among juvenile alligators from two well-studied populations (Lake Apopka and Lake Woodruff) in north central Florida. These studies obtained alligators of similar size from each lake under the assumption that the animals were of similar age. Lake Apopka is a hypertrophic lake with a 50-year history of contamination from agricultural and municipal operations, whereas Lake Woodruff is a eutrophic lake and part of a National Wildlife Refuge that receives little point source pollution. If growth rates differ among these areas, it could be argued that differences in endocrine parameters reported previously (e.g. steroid or thyroid hormone concentrations) could be the result of differences in the animals' ages. Using growth annuli in cross-sections of femurs, we estimated the ages of juvenile alligators and compared the relationship of estradiol-17beta (E(2)) and testosterone (T) to size and age within each lake and sex. No differences were detected in the relationship between size and age between the two areas indicating similar growth rates between lakes. Plasma E(2) was positively related to size in females from Lake Apopka, and age in Woodruff females. Males from Lake Apopka had elevated plasma E(2) compared with Lake Woodruff males and did not differ from Woodruff females. No significant relationships were detected for T from either lake, and no differences in plasma T were detected among lakes or sexes. Our data indicate that both size and age can have a significant relationship with steroid concentrations. However, the relationship between steroid concentrations and size or age differed between lakes. We suggest both factors should be considered when conducting physiological studies where there is evidence to suggest growth rates may differ among populations.
    • "Resident alligators displayed altered production of sex-steroids and other hormones , which was suspected to have derailed proper development. Further, many abnormities persisted into later life stages (Crain et al., 1998Crain et al., , 1997 Gregory et al., 1996; Guillette et al., 1999a Guillette et al., , 1994 Guillette et al., , 1996 Guillette et al., 1999b; Gunderson et al., 2001; Milnes et al., 2002; Pickford et al., 2000). Although Lake Apopka was a principal study site, Dr. Guillette and colleagues investigated a number of other contaminated locales including cattle feedlots, munitions sites, Florida Springs, the Florida Everglades, agricultural areas, Kennedy Space Center, Merritt Island National Wildlife Refuge, and sites near Kraft paper mills or sewage treatment plants (Bowden et al., 2014; Edwards and Guillette, 2006; Edwards et al., 2006a; Folmar et al., 2001 Folmar et al., , 1996 McCoy et al., 2008; Nilsen et al., 2016; Orlando et al., 2004; Soto et al., 2004). "
    [Show abstract] [Hide abstract] ABSTRACT: Dr. Louis J. Guillette Jr. thought of himself as a reproductive biologist. However, his interest in reproductive biology transcended organ systems, life history stages, species, and environmental contexts. His integrative and collaborative nature led to diverse and fascinating research projects conducted all over the world. He doesn't leave us with a single legacy. Instead, he entrusts us with several. The purpose of this review is to highlight those legacies, in both breadth and diversity, and to illustrate Dr. Guillette's grand contributions to the field of reproductive biology. He has challenged the field to reconsider how we think about our data, championed development of novel and innovative techniques to measure endocrine function, helped define the field of endocrine disruption, and lead projects to characterize new endocrine disrupting chemicals. He significantly influenced our understanding of evolution, and took bold and important steps to translate all that he has learned into advances in human reproductive health. We hope that after reading this manuscript our audience will appreciate and continue Dr. Guillette's practice of open-minded and passionate collaboration to understand the basic mechanisms driving reproductive physiology and to ultimately apply those findings to protect and improve wildlife and human health.
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