Cytotoxic Action of Acetyl-11-keto-β-Boswellic Acid (AKBA) on Meningioma Cells
Dongguk University, Sŏul, Seoul, South Korea Planta Medica
(Impact Factor: 2.15).
06/2002; 68(5):397-401. DOI: 10.1055/s-2002-32090
Acetyl-11-keto-beta-boswellic acid (AKBA) is a naturally occurring pentacyclic triterpene isolated from the gum resin exudate of the tree Boswellia serrata (frankincense). Because pentacyclic triterpenes have antiproliferative and cytotoxic effects against different tumor types, we investigated whether AKBA would act in a similar fashion on primary human meningioma cell cultures. Primary cell cultures were established from surgically removed meningioma specimens. The number of viable cells in the absence/presence of AKBA was determined by the non-radioactive cell proliferation assay. The activation status of the proliferative cell marker, extracellular signal-regulated kinase-1 and -2 (Erk-1 and Erk-2) was determined by immunoblotting with the antibody that recognizes the activated form of these proteins. Treatment of meningioma cells by AKBA revealed a potent cytotoxic activity with half-maximal inhibitory concentrations in the range of 2 - 8 microM. At low micromolar concentrations, AKBA rapidly and potently inhibited the phosphorylation of Erk-1/2 and impaired the motility of meningioma cells stimulated with platelet-derived growth factor BB. The cytotoxic action of AKBA on meningioma cells may be mediated, at least in part, by the inhibition of the Erk signal transduction pathway. Because of the central role the Erk pathway plays in signal transduction and tumorigenesis, further investigation into the potential clinical use for AKBA and related boswellic acids is warranted.
Available from: Mohammad Ahmed Khan
- "BioMed Research International cells  "
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ABSTRACT: The study investigated the growth-inhibiting and apoptosis mediating effects of B. serrata extract as monotherapy and combination therapy with DOX against hepatocellular carcinoma cell lines. Boswellic acid rich fraction of B. serrata extract was prepared. MTT assay on HepG2 and Hep3B cells was carried out using B. serrata alone and in combination with DOX. Further, caspase-3 activity, TNF-α level, and IL-6 level were estimated. Isobolographic analysis was carried out to evaluate the effect of combination therapy. Additionally, protective effect of B. serrata extract on DOX induced hepatic toxicity was also evaluated in Wistar rats. B. serrata extract inhibited growth of HepG2 (IC50 value of 21.21 ± 0.92 μg/mL) as well as HepG2 (IC50 value of 18.65 ± 0.71 μg/mL). DOX inhibited growth in HepG2 and Hep3B cells with an IC50 of 1.06 ± 0.04 μg/mL and 1.92 ± 0.09 μg/mL. Isobolographic analysis showed combination index (CI) of DOX and B. serrata extract of 0.53 ± 0.03 to 0.79 ± 0.02 suggesting synergistic behavior against the two cell lines. B. serrata extract also caused dose dependent increase in caspase-3 activity, TNF-α level, and IL-6 level which was higher (P < 0.001) with DOX (1 μM) and B. serrata extract (20 μg/mL) combination. B. serrata extract also protected Wistar rats against DOX induced hepatic toxicity.
Available from: Mahmoud M Suhail
- "The frankincense essential oil possesses anti-proliferative and pro-apoptotic activities against multiple human cancer cell lines in vitro and in vivo[6-8]. Boswellic acids were found to be the major components in frankincense extracts, with anti-tumor activity owing to their cytostatic and pro-apoptotic properties in multiple human cancer cell lines including meningioma cells , leukemia cells , hepatoma cells , melanoma cells, fibrosarcoma cells , colon cancer cells , and prostate cancer cells [14-16]. Some of the effects of frankincense essential oil were found to be related to the activities of sesquiterpenes and diterpenes . "
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Frankincense (Boswellia carterii, known as Ru Xiang in Chinese) and sandalwood (Santalum album, known as Tan Xiang in Chinese) are cancer preventive and therapeutic agents in Chinese medicine. Their biologically active ingredients are usually extracted from frankincense by hydrodistillation and sandalwood by distillation. This study aims to investigate the anti-proliferative and pro-apoptotic activities of frankincense and sandalwood essential oils in cultured human bladder cancer cells.
The effects of frankincense (1,400–600 dilutions) (v/v) and sandalwood (16,000–7,000 dilutions) (v/v) essential oils on cell viability were studied in established human bladder cancer J82 cells and immortalized normal human bladder urothelial UROtsa cells using a colorimetric XTT cell viability assay. Genes that responded to essential oil treatments in human bladder cancer J82 cells were identified using the Illumina Expression BeadChip platform and analyzed for enriched functions and pathways. The chemical compositions of the essential oils were determined by gas chromatography–mass spectrometry.
Human bladder cancer J82 cells were more sensitive to the pro-apoptotic effects of frankincense essential oil than the immortalized normal bladder UROtsa cells. In contrast, sandalwood essential oil exhibited a similar potency in suppressing the viability of both J82 and UROtsa cells. Although frankincense and sandalwood essential oils activated common pathways such as inflammatory interleukins (IL-6 signaling), each essential oil had a unique molecular action on the bladder cancer cells. Heat shock proteins and histone core proteins were activated by frankincense essential oil, whereas negative regulation of protein kinase activity and G protein-coupled receptors were activated by sandalwood essential oil treatment.
The effects of frankincense and sandalwood essential oils on J82 cells and UROtsa cells involved different mechanisms leading to cancer cell death. While frankincense essential oil elicited selective cancer cell death via NRF-2-mediated oxidative stress, sandalwood essential oil induced non-selective cell death via DNA damage and cell cycle arrest.
Available from: Jehad M Yousef
- "These compounds have been previously reported to have inhibitory effect on pro-inflammatory processes by their effects on 5-lipooxygenase and cyclo-oxygenase and on the complement system (Knaus and Wagner 1996, Ammon, 2008). Boswellic acid was found to have anti-proliferative activities toward a variety of malignant cells and used as a therapeutic agent in the treatment of non-insulin dependent diabetes mellitus (Park et al., 2002; Kim et al., 2008; and Ni et al., 2012). However, previous published data revealed some adverse effect of Frankincense. "
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ABSTRACT: The present study evaluated the safety and the hypolipidemic impact of aqueous extracts of some medicinal herbs namely, Salvia officinalis (sage) leaf extract, Alpinia galanga (galangal) rhizome extract, and gum olibanum extract of Boswellia serrata (frankincense) on heart of rats.. Creatin phophokinase (CPK), lactate dehydrogenase (LDH) and glucose were assessed in heart tissue. Results revealed that oral ingestion of sage aqueous extracts to rats for 30 consecutive days showed non significant change in heart parameters. Meanwhile, galangal extract markedly induced elevation in both enzyme activities as well as in the glucose content of cardiac tissue when compared with control animals. Frankincense aqueous extract showed an adverse effect on heart tissue which ensured by marked decrease in CPK, LDH and glucose in heart tissue. Serum cholesterol and triacyglycerol (TGs) in rats were also investigated to evaluate the hypolipidemic efficacy of the tested herb extracts. The result showed that sag extract has no effect on serum cholesterol but it has lowering effect on serum TGs level. However, galangal extract has lowering effect on serum cholesterol and non significant change was observed in serum TGs level versus control rats. Frankincense extract has no effect on serum lipid profiles when compared to control animals. Overall, sag and galangal aqueous extracts does not adversely influence rat heart coupled with their beneficial hypolipidemic capacity, however frankincense extract showed an adverse effect on heart tissue and precautions must be taken during it's us as a traditional herbal medicine.
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