Determination of irbesartan in the presence of hydrochlorothiazide by derivative spectrophotometry

Department of Analytical Chemistry, Faculty of Chemistry, University of Murcia, 30071 Murcia, Spain.
Journal of Pharmaceutical and Biomedical Analysis (Impact Factor: 2.98). 07/2002; 29(1-2):299-305. DOI: 10.1016/S0731-7085(02)00073-0
Source: PubMed


A first-derivative spectrophotometric method for the determination of irbesartan (IRB) alone and in the presence of hydrochlorothiazide (HCT) is described. Measurements are made at the zero-crossing wavelength at 263.0 nm for IRB. The calibration graph was linear over the range 1.0-12.0 mg l(-1) of IRB, detection limit was 0.15 mg l(-1). HCT, in the presence of IRB was determined by direct spectrophotometric method at 317 nm. The calibration graph was linear over the range 2.0-50.0 mg l(-1) of HCT, detection limit was 0.25 mg l(-1). The proposed methods were successfully applied to the determinations of IRB and HCT in combined tablets.

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    • "), 543-548. [13].AlberoI.,RódenasV.,GarcíaS.,Sánchez-PedreñoC., "
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    ABSTRACT: A simple and accurate method is presented for determination of irbesartan in pure form and commercial dosage forms. The method is based on the reaction of the above cited drug with bromophenol blue (BPB), bromocresol purple (BCP), bromothymol blue (BTB) and naphthol blue black (NBB) dyes in solutions containing Britton buffer to form ion-pair complexes extractable with chloroform and subsequently measured spectrophotometrically at 420nm for BPB, BCP, BTB and 625nm for NBB. All the reaction conditions for the proposed method have been studied. The reactions were extremely rapid at room temperature and the absorbance values remains unchanged up to 24hrs. Beer's law was obeyed in the ranges 1.8-45, 1.5-43, 2-60 and 10-244 g/mL for BPB, BCP, BTB and NBB, respectively. Interferences of the other ingredients and excipients were not observed. The proposed method is simple, fast and sensitive, and the first reported extractive method for the determination of irbesartan.
    Full-text · Article · Jan 2006
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    ABSTRACT: A second-derivative spectrophotometric method for the simultaneous determination of irbesartan (IRB) and hydrochlorothiazide (HCT) in tablets is described. Measurements were made at the zero-crossing wavelengths at 230.1 nm for IRB and 232.7 nm for HCT. The calibration graphs were linear in the range of 14.4–33.6 mg L for IRB and 1.2–2.8 mg L for HCT. The limits of quantitation were 5.0 and 1.1 mg L. The proposed method was successfully applied to the simultaneous determination of IRB and HCT in commercial tablets with a high percentage of recovery, good accuracy, and precision.
    No preview · Article · Jan 2003 · Analytical Letters
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    ABSTRACT: Derivative spectrophotometry is an analytical technique of great utility for extracting both qualitative and quantitative information from spectra composed of unresolved bands, and for eliminating the effect of baseline shifts and baseline tilts. It consists of calculating and plotting one of the mathematical derivatives of a spectral curve. Thus, the information content of a spectrum is presented in a potentially more useful form, offering a convenient solution to a number of analytical problems, such as resolution of multi-component systems, removal of sample turbidity, matrix background and enhancement of spectral details. Derivative spectrophotometry is now a reasonably priced standard feature of modern micro-computerized UV/Vis spectrophotometry.The instrumental development and analytical applications of derivative UV/Vis regions absorption spectrophotometry produced in the last 10 years (since 1994) are reviewed.
    Full-text · Article · Aug 2004 · Analytica Chimica Acta
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