Jiang Y, Jahagirdar BN, Reinhardt RL, et al. Pluripotency of mesenchymal stem cells derived from adult marrow. Nature 418(6893): 41

Stem Cell Institute, University of Minnesota Medical School, Minneapolis, Minnesota 55455, USA.
Nature (Impact Factor: 41.46). 08/2002; 418(6893):41-9. DOI: 10.1038/nature00870
Source: PubMed


We report here that cells co-purifying with mesenchymal stem cells--termed here multipotent adult progenitor cells or MAPCs--differentiate, at the single cell level, not only into mesenchymal cells, but also cells with visceral mesoderm, neuroectoderm and endoderm characteristics in vitro. When injected into an early blastocyst, single MAPCs contribute to most, if not all, somatic cell types. On transplantation into a non-irradiated host, MAPCs engraft and differentiate to the haematopoietic lineage, in addition to the epithelium of liver, lung and gut. Engraftment in the haematopoietic system as well as the gastrointestinal tract is increased when MAPCs are transplanted in a minimally irradiated host. As MAPCs proliferate extensively without obvious senescence or loss of differentiation potential, they may be an ideal cell source for therapy of inherited or degenerative diseases.

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    • "In 2002 the group of Catherine Verfaillie described the cells they called multipotent adult progenitor cells (MAPC) (Jiang et al., 2002). Those cells, obtained after a very long-term in vitro culture, were able to differentiate into all cell types and even to complement blastocyst (Jiang et al., 2002), what indicated they were really pluripotent . However, this study could not be reproduced by several independent groups (Check, 2007). "
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    ABSTRACT: Stem cells are self-renewing cells that can differentiate into specialized cell type(s). Pluripotent stem cells, i.e. embryonic stem cells (ESC) or induced pluripotent stem cells (iPSC) differentiate into cells of all three embryonic lineages. Multipotent stem cells, like hematopoietic stem cells (HSC), can develop into multiple specialized cells in a specific tissue. Unipotent cells differentiate only into one cell type, like e.g. satellite cells of skeletal muscle. There are many examples of successful clinical applications of stem cells. Over million patients worldwide have benefited from bone marrow transplantations performed for treatment of leukemias, anemias or immunodeficiencies. Skin stem cells are used to heal severe burns, while limbal stem cells can regenerate the damaged cornea. Pluripotent stem cells, especially the patient-specific iPSC, have a tremendous therapeutic potential, but their clinical application will require overcoming numerous drawbacks. Therefore, the use of adult stem cells, which are multipotent or unipotent, can be at present a more achievable strategy. Noteworthy, some studies ascribed particular adult stem cells as pluripotent. However, despite efforts, the postulated pluripotency of such events like "spore-like cells", "very small embryonic-like stem cells" or "multipotent adult progenitor cells" have not been confirmed in stringent independent studies. Also plasticity of the bone marrow-derived cells which were suggested to differentiate e.g. into cardiomyocytes, has not been positively verified, and their therapeutic effect, if observed, results rather from the paracrine activity. Here we discuss the examples of recent studies on adult stem cells in the light of current understanding of stem cell biology.
    Full-text · Article · Jul 2015 · Acta biochimica Polonica
    • "In this study, evaluation of the cell markers of CD73, CD34 and CD45 at passage 4 was done by RT-PCR showing that the ADSCs were positive for CD73 and negative for CD34 and CD45. These markers confirmed the mesenchymal nature of the isolated cells (Jiang et al. 2002; Dominici et al. 2006). "
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    ABSTRACT: Subcutaneous adipose-derived stem cells (ADSCs) can be provided in large quantities as an alternative source in regenerative medicine. We report osteogenic potential of these cells in rabbits. Four gram subcutaneous adipose tissue samples from anaesthetized adult Dutch white rabbits were digested in collagenase type II and cultured in DMEM. Cells were enumerated up to the 4th passage and population doubling time (PDT) was determined. Karyotyping and RT-PCR were used to evaluate normal diploid cells and expression of ADSCs or hematopoietic stem cell markers including CD73, CD34 and CD45. Adipogenic and osteogenic potential of differentiated cells was confirmed by Oil Red O and Alizarin Red S staining. Isolated cells had fibroblastic like morphology with an S shaped growth and a PDT of 70.2 hours. Until the 4th passage cells had normal a karyotype. RT- PCR showed that the cells were CD73 positive but CD34 and CD45 negative. With special staining, evidence of calcium deposition and formation of fat cells confirmed osteogenic and adipogenic properties. These findings suggest osteogenic potential of subcutaneous ADSCs for application in regenerative medicine and tissue engineering.
    No preview · Article · Jul 2015
    • "Mesenchymal stem cells (MSCs) are known as the stem cell source for stem cell–based bone regeneration (Jiang et al. 2002) and are generally isolated from not only bone marrow (BM) but also fat, cord blood, and even peripheral blood (PB). Bone marrow–derived mesenchymal stem cells (BMMSCs) have been considered a source for regenerating bone defects (Kuznetsov et al. 2001), but the method of BM aspiration from patients was aggressed to the donor sites (Bain 2005). "
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    ABSTRACT: Peripheral blood (PB) is known as a source of mesenchymal stem cells (MSCs), as is bone marrow (BM), and is acquired easily. However, it is difficult to have enough MSCs, and their osteogenic capacity with dental implantations is scarce. Therefore, we characterized peripheral blood mesenchymal stem cells (PBMSCs) cultured on a bone marrow-derived mesenchymal stem cell (BMMSC) natural extracellular matrix (ECM) and demonstrated the osteogenic capability in an experimental chamber implant surgery model in rabbits. We isolated PBMSCs from rabbits by culturing on a natural ECM-coated plate during primary culture. We characterized the PBMSCs using a fluorescence-activated cell scanner, cell proliferation assay, and multiple differentiation assay and compared them with BMMSCs. We also analyzed the osteogenic potential of PBMSCs mixed with hydroxyapatite/tricalcium phosphate (HA/TCP) by transplanting them into immunocompromised mice. Then, the mixture was applied to the canals. After 3 and 6 wk, we analyzed new bone (NB) formation inside the chambers using histological and histomorphometric analyses. The PBMSCs had a similar rate of BrdU-positive cells to BMMSCs, positively expressing CD90 but negative for CD14. The PBMSCs also showed osteogenic, adipogenic, and chondrogenic ability in vitro and osteogenic ability in vivo. Histological and histomorphometric results illustrated that the PBMSC and BMMSC groups showed higher NB than the HA/TCP and defect groups in the upper and lower chambers at 6 wk and in the upper canal at 3 wk; however, there was no difference in NB among all groups in the lower canal at 3 wk. The PBMSCs have characteristics and bone regeneration ability similar to BMMSCs both in vitro and in vivo. ECM was effective for obtaining PBMSCs. Therefore, PBMSCs are a promising source for bone regeneration for clinical use. © International & American Associations for Dental Research 2015.
    No preview · Article · Jun 2015 · Journal of dental research
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