Development of a Larval Bioassay for Susceptibility of Cat Fleas (Siphonaptera: Pulicidae) to Imidacloprid

Auburn University, AUO, Alabama, United States
Journal of Medical Entomology (Impact Factor: 1.95). 08/2002; 39(4):671-4. DOI: 10.1603/0022-2585-39.4.671
Source: PubMed


Strategies for controlling cat fleas, Ctenocephalidesfelisfelis (Bouché), have undergone dramatic changes in the past 5 yr. With the advent of on-animal treatments with residual activity the potential for the development of insecticide resistance increases. A larval bioassay was developed to determine the baseline susceptibility of field-collected strains of cat fleas to imidacloprid. All four laboratory strains tested showed a similar level of susceptibility to imidacloprid. Advantages of this bioassay are that smaller numbers of fleas are required because flea eggs are collected for the test. Insect growth regulators and other novel insecticides can also be evaluated. Using a discriminating dose, the detection of reduced susceptibility in field strains can be determined with as few as 40 eggs.

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Available from: Ian Denholm, Jul 20, 2015
    • "However, the activity of the insecticide varied greatly depending on the method used to test for resistance. A larval bioassay with imidacloprid and fipronil has provided consistent results (Rust et al. 2002, 2014), and topical application of insecticides on the cuticle of adult fleas, which enables the testing of individual fleas, has also provided reliable levels of sensitivity (Moyses 1995). Topical application bioassays of 13 insecticides to individuals of a single flea strain were reported by Moyses and Gfellar (2001). "
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    ABSTRACT: The susceptibility of 12 field-collected isolates and 4 laboratory strains of cat fleas, Ctenocephalides felis was determined by topical application of some of the insecticides used as on-animal therapies to control them. In the tested field-collected flea isolates the LD50 values for fipronil and imidacloprid ranged from 0.09 to 0.35 ng/flea and 0.02 to 0.19 ng/flea, respectively, and were consistent with baseline figures published previously. The extent of variation in response to four pyrethroid insecticides differed between compounds with the LD50 values for deltamethrin ranging from 2.3 to 28.2 ng/flea, etofenprox ranging from 26.7 to 86.7 ng/flea, permethrin ranging from 17.5 to 85.6 ng/flea, and d-phenothrin ranging from 14.5 to 130 ng/flea. A comparison with earlier data for permethrin and deltamethrin implied a level of pyrethroid resistance in all isolates and strains. LD50 values for tetrachlorvinphos ranged from 20.0 to 420.0 ng/flea. The rdl mutation (conferring target-site resistance to cyclodiene insecticides) was present in most field-collected and laboratory strains, but had no discernible effect on responses to fipronil, which acts on the same receptor protein as cyclodienes. The kdr and skdr mutations conferring target-site resistance to pyrethroids but segregated in opposition to one another, precluding the formation of genotypes homozygous for both mutations.
    No preview · Article · Jul 2015 · Parasitology Research
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    • "The KS1 strain of Ctenocephalides felis, which was collected from dogs and cats in a Kansas shelter in 1990 and has since been maintained in a laboratory, has documented resistance or natural reduced susceptibility to carbaryl, chlorpyrifos, fenthion, fipronil, imidacloprid, permethrin, pyrethrins, and spinosad [23,31,32,48-52]. Based on bioassay and genetic analysis the cause of reduced efficacy of pyrethroid- and organophosphate-based products with this strain is likely true resistance [32,48,49]. "
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    ABSTRACT: This review defines insecticide/acaricide resistance and describes the history, evolution, types, mechanisms, and detection of resistance as it applies to chemicals currently used against fleas and ticks of dogs and cats and summarizes resistance reported to date. We introduce the concept of refugia as it applies to flea and tick resistance and discuss strategies to minimize the impact and inevitable onset of resistance to newer classes of insecticides. Our purpose is to provide the veterinary practitioner with information needed to investigate suspected lack of efficacy, respond to lack of efficacy complaints from their clients, and evaluate the relative importance of resistance as they strive to relieve their patients and satisfy their clients when faced with flea and tick infestations that are difficult to resolve. We conclude that causality of suspected lack of insecticide/acaricide efficacy is most likely treatment deficiency, not resistance.
    Full-text · Article · Jan 2014 · Parasites & Vectors
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    • "The basis of the monitoring undertaken by this group is a validated imidacloprid larval development inhibition assay (Rust et al. 2002), for which a diagnostic dose (DD) has been determined to facilitate applicability to high-throughput field isolate screening (Rust et al. 2005). While the concept of utilising laboratory bioassays for monitoring parasiticide responses is not novel, the geographic scope, high degree of co-ordination and longevity of this monitoring initiative make it unique, particularly within the field of veterinary parasitology. "
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    ABSTRACT: In 2001, an international surveillance initiative was established, utilising a validated larval development inhibition assay to track the susceptibility of cat flea isolates to imidacloprid. In 2009, an Australian node was incorporated into the programme, joining laboratories in the United States and Europe. Field isolates of Ctenocephalides felis eggs were submitted to participating laboratories and, where egg quantity and quality was sufficient, were placed in the imidacloprid discriminating dose bioassay for evaluation. Between 2002 and 2012, a total of 2,307 cat flea isolates were received across all sites; 1,685 submissions (73%) were suitable for placement into the bioassay. In the Northern Hemisphere, isolate submission rate was influenced by season, with highest numbers submitted between June and October. In Australia, pets with flea infestations could be sourced year-round, and submission rate was largely influenced by programme factors and not climate. A total of 1,367 valid assays were performed between 2002 and 2012 (assay validity data was not recorded in 2001); adult flea emergence 5 % or greater at 3 ppm imidacloprid was observed in 38 of these assays (2.8%). For these isolates that reached the threshold for further investigation, re-conduct of the assay using either a repeat challenge dose of 3 ppm of imidacloprid or a dose response probit analysis confirmed their susceptibility to imidacloprid. From 2009 to 2012, the Australian node performed valid assays on 97 field isolates from a total of 136 submissions, with no adult emergence observed at the 3-ppm imidacloprid discriminating dose. In addition to reviewing the data generated by this twelve-year initiative, this paper discusses lessons learned from the coordination and evolution of a complex project across geographically dispersed laboratories on three continents.
    Full-text · Article · Jun 2013 · Parasitology Research
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