Incomplete Chromosome Exchanges are Not Fingerprints of High LET Neutrons

ArticleinRadiation Protection Dosimetry 99(1-4):215-6 · February 2002with4 Reads
Impact Factor: 0.91 · DOI: 10.1093/oxfordjournals.rpd.a006765 · Source: PubMed

    Abstract

    A new fluorescence in situ hybridisation (FISH) technique combining whole chromosome specific DNA libraries with pan-centromeric DNA and telomeric PNA
    probes was introduced to investigate the induction of chromosome exchanges in human lymphocytes after exposure to low (4 Gy
    X rays) and high (1 Gy neutrons) linear energy transfer radiation. This combination of probes allowed accurate detection of
    exchange aberrations involving the painted chromosomes and an unambiguous discrimination between complete and incomplete exchanges,
    as well as terminal and interstitial deletions. Data obtained in the present study using combined FISH assay with telomeres
    detection showed no differences between two types of radiation regarding the induction of incomplete exchanges.