Induction of micronuclei by zinc in human leukocytes: A study using cytokinesis-block micronucleus assay

ArticleinBiological Trace Element Research 88(2):139-44 · September 2002with12 Reads
Impact Factor: 1.75 · DOI: 10.1385/BTER:88:2:139 · Source: PubMed

    Abstract

    In the present study, we report the results of the capability of zinc chloride for the induction of micronuclei in cultured human leukocytes using cytokinesis-block micronucleus assay. Two concentrations of zinc chloride (1.5 x 10(-4) M and 3.0 x 10(-4) M) were used to evaluate the potential of this zinc salt to induce micronucleus formation. This effect was compared with positive (mitomycin C treated) and negative controls (no salt added). Our results show a significant (p < or = 0.001) increase of micronucleated cytokinesis-blocked cells (MNCBs) in zinc-chloride-treated cells compared to the negative control. Induction of MNCBs was not in a dose-dependent manner for zinc chloride concentrations tested. This report is the first to describe the efficiency of cytokinesis-block micronucleus assay to evaluate the genotoxic effects of zinc salt.