Inhibitory effect of corynoline isolated from the aerial parts of Corydalis incisa on the acetylcholinesterase

College of Pharmacy, Woosuk University, Samrye 565-701, Korea.
Archives of Pharmacal Research (Impact Factor: 2.05). 01/2003; 25(6):817-9. DOI: 10.1007/BF02976997
Source: PubMed


In the course of screening Korean natural products for acetylcholinesterase (AChE) inhibitory activity, it was found that a methanolic extract of the aerial parts of Corydalis incisa (Papaveraceae) showed significant inhibitory effects on AChE. Corynoline isolated from this plant inhibited AChE activity in a dose-dependent manner, and the IC50 value of corynoline was 30.6 microM. The AChE inhibitory activity of corynoline was reversible and noncompetitive.

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    • "Triterpenoid alkaloids, steroidal alkaloids, indole alkaloids, isoquinoline alkaloid, and lycopodane-type alkaloid are the major types of alkaloids having significant anticholinesterase activity making them promising candidates to be used as cholinesterase inhibitors in clinical practice. Most of the compounds having potential anticholinesterase activity are isolated from Buxaceae, Amaryllidaceae, Lycopodiaceae, Lamiaceae, Chenopodiaceae, Papaveraceae, Apocynaceae, and Labiatae species.[1317182527294559] Following are three of the imporant families having potential compounds to be used as anticholonesterase inhibitors. "
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    ABSTRACT: Alzheimer's disease (AD) is a progressive neurodegenerative disease, wherein a progressive loss of cholinergic synapses occurs in hippocampus and neocortex. Decreased concentration of the neurotransmitter, acetylcholine (ACh), appears to be critical element in the development of dementia, and the most appropriate therapeutic approach to treat AD and other form of dementia is to restore acetylcholine levels by inhibiting both major form of cholinesterase: Acetylcholinesterase (AChE) and butyrylcholinesterase (BChE). Consequently, researches have focused their attention towards finding cholinesterase inhibitors from natural products. A large number of such inhibitors have been isolated from medicinal plants. This review presents a comprehensive account of the advances in field of cholinesterase inhibitor phytoconstituents. The structures of some important phytoconstituents (collected through are also presented and the scope for future research is discussed.
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    • "Therefore , early investigation of the CYP inhibitory potential and identification of CYP isoforms involved in the metabolism of NCEs are obviously necessary for the discovery of efficacious and safe drugs. Corynoline (Fig. 1), an isoquinoline alkaloid isolated from the genus Corydalis, has been demonstrated to exert multiple pharmacological activity including inhibition of acetylcholinesterase (Kim, 2002), inhibition of cell adhesion (Kamigauchi et al., 2005), fungitoxic and cytotoxic activity (Ma et al., 1999; Choi et al., 2007). "
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    • "Structure–activity relationship suggested that the nitrogen substituents at C-3 and/or C-20 of steroidal skeleton and the hydrophobic properties of the pregnane skeleton are the key structural features contributed to the inhibitory potency of pregnane-type steroidal alkaloids against AChE (Khalid et al., 2004). Bioassay-guided fractionation of the methanolic extract resulted in the isolation of three furanocoumarins , isoimperatorin, imperatorin and oxypeucedanin as active principles from the methanolic extract of the roots of Angelica dahurica, which inhibited AChE activity in a dose-dependent manner (Kim et al., 2002). In a bioassay-guided search for AChE inhibitors four isoquinoline alkaloids, corynoxidine, protopine, palmatine and berberine have been isolated from the methanolic extract of the aerial parts of Corydalis speciosa (Kim et al., 2004). "
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