Development and application of a genome specific PCR marker for Haynaldia villosa

College of Crop Science, China Agricultural University, Beijing 100094, China.
Acta Genetica Sinica 05/2003; 30(4):350-6.
Source: PubMed


Random amplified polymorphic DNA (RAPD) analysis was performed on common wheat Chinese Spring, H. villosa, addition lines of H. villosa chromosome in CS, substitution line 3V of H. villosa chromosome in Triticum aestivum. A genome specific polymorphic DNA segment from H. villosa, OPF02757, was obtained. On the basis of cloning and sequencing of OPF02757, two PCR primers were designed and a genome specific PCR marker for H. villosa was established. The PCR marker including 677 bp was localized on all the seven pairs of H. villosa chromosomes. The result of PCR amplification by the primers indicated that there was a specific band of 677 bp in the materials containing H. villosa Chromosome such as T. aestivum-H. villosa addition, T. aestivum-H. villosa substitution, T. aestivum-H. villosa amphidiploid, T. durum-H. villosa amphidiploid and H. villosum from different accessions, and there was no specific band of 677 bp if the materials did not contain H. villosa chromosome, such as T. aestivum, T. durum, Secale cereale, Hordeum vulgare, Thinopyrum elongatum, Thinopyrum intermedium. Therefore, the PCR maker of 677 bp is specific to H. villosa genome, and could be used as molecular marker for detection of chromosomes of H. villosa in wheat.

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    • "villosa lines (Chen et al. 1996b), to show the presence H. villosa chromatin in sexual hybrids (Zhang et al. 1998), as well as to identify the hybrid calli obtained from asymmetric somatic hybridization (Xia et al. 1998). Moreover, a specific RAPD marker found by Liu et al. (2003) could be used for detection of H. villosa chromatin in other genetic backgrounds. Specific RAPD/SCAR or RAPD markers that can be used for easy screening in wheat for presence/absence of resistance genes have been obtained. "
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    ABSTRACT: Dasypyrum villosum (L.) P. Candargy is discussed as a species commonly used in wheat improvement. Chromosomal localization of the potentially useful traits and chromosomal position of some morphological and isozyme markes are shown. The investigations using molecular RAPD, AFLP, SSR, RFLP markers and in situ (GISH, FISH) hybridization experiments on D. villosum itself and in wide hybrids with Triticum are summarized. The article also presents the information about designation of D. villosum chromosomes and the current knowledge on the phenomenon of two-coloured D. villosum caryopses.
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    Article: Dasypyrum
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