Nucleotide sequence and phyogenetic classification of candidate human papilloma virus type 92

Department of Medical Microbiology, Malmö University Hospital, Lund University, Malmö, Sweden.
Virology (Impact Factor: 3.32). 09/2003; 312(2):255-60. DOI: 10.1016/S0042-6822(03)00391-X
Source: PubMed


From a basal cell carcinoma (BCC) the complete genome of candidate human papillomavirus (HPV) type 92 was characterized. Phylogenetically, the candidate HPV 92 was relatively distantly related to other cutaneous HPV types within the B1 group. By quantitative real time PCR, 94 viral copies were present per cell in the BCC and another BCC contained 1 viral copy per cell. Lower copy numbers were found in two solar keratoses (1 copy per 33 cells and 1 copy per 60 cells) and two squamous cell carcinomas (1 copy per 436 cells and 1 copy per 1143 cells). The high viral load of HPV 92 in two BCCs differs from the low amount of HPV DNA reported from nonmelanoma skin cancers.

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Available from: Annika Antonsson, Sep 28, 2015
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    • "HPV-96 belongs to Betapapillomavirus species 5 and was originally isolated from a SCC of the skin [18]. The next phylogenetically closest genotype to HPV-150 and HPV-96 is HPV-92, which belongs to Betapapillomavirus species 4, and was originally isolated from a BCC of the skin [16]. Since these three genotypes seem to have evolved through intra-host duplication [43] and two of them show at least some carcinogenic potential, the ancestral genotype from which these genotypes were derived may have itself been an oncogenic HPV genotype. "
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    Full-text · Article · Jul 2011 · PLoS ONE
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    • "Although most cutaneous samples contain only low amounts (b1 copy/cell) of known viruses (Hazard et al., 2006; Vasiljevic et al., 2008; Weissenborn et al., 2005), there are many examples of skin lesions that do contain high amounts of virus per cell, e.g. HPV1 and 2 (Gissmann et al., 1977; Orth et al., 1977) in skin warts, HPV5 and 8 in skin cancers from epidermodysplasia verucciformis patients (Ostrow et al., 1982; Pfister et al., 1981), HPV92 in a BCC (Forslund et al., 2003) and HPV88 (Kullander et al., 2008) in an SCC. Fig. 1. "
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    ABSTRACT: To expand our knowledge of the genomic diversity of human papillomaviruses (HPVs), we searched for new HPVs in squamous cell carcinomas of the skin (SCC) and seemingly HPV-negative, otherwise typically HPV-associated lesions. We describe the characterization of three novel HPV types. HPV109 was isolated from an SCC, HPV112 from a condyloma and HPV114 from a low-grade cervical lesion. Pairwise alignment of the L1 sequences classified HPV114 to genus alpha species 3, whereas HPV112 defined a new species in the genus gamma. HPV109 had uncertain classification because of a low and about equal similarity in the L1 gene (between 60% and 65%) to different genera. Type-specific real-time PCRs of cervical samples, a majority from women with low grade atypical cytology, (n = 2856) and various cutaneous samples (n = 538), found HPV114 in 1.7% (48/2856) of the genital samples, whereas both HPV109 and 112 were rare viruses found at high viral loads only in their index samples.
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    • "Taking advantage of the rolling-circle amplification method (Rector et al., 2004), the number of isolated PV types has increased over the past few years. Approximately 150 PV genomes, isolated from humans and different animal species, have been cloned and completely sequenced so far (Van Ranst et al., 1992; Terai et al., 2002; Forslund et al., 2003; Rector et al., 2007). However, the number of known non-human host species is still low (approx. "
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