Project

iPASTIC: an online toolkit to estimate plant abiotic stress indices

Goal: Development an online toolkit to calculate plant abiotic stress indices including the tolerance index (TOL), relative stress index (RSI), mean productivity (MP), harmonic mean (HM), yield stability index (YSI), geometric mean productivity (GMP), stress susceptibility index (SSI), stress tolerance index (STI), and yield index (YI).

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Alireza Pour-Aboughadareh
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Alireza Pour-Aboughadareh
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Premise of the Study: In crop breeding programs, breeders use yield performance in optimal and stressful environments as key indicator for screening the most tolerant genotypes. Over four decades, several yield-based indices have been suggested for evaluating stress tolerance in crops. Despite the well-established use of these indices in agronomy and plant breeding, a user-friendly software that would provide access to these methods is still lacking. Methods and Results: The Plant Abiotic Stress Index Calculator (iPASTIC) is an online program based on Javascript and R, which calculates common stress tolerance and susceptibility indices for various crop traits including the tolerance index (TOL), relative stress index (RSI), mean productivity (MP), harmonic mean (HM), yield stability index (YSI), geometric mean productivity (GMP), stress susceptibility index (SSI), stress tolerance index (STI), and yield index (YI). Along with these indices, this easily accessible tool can also calculate their ranking patterns, estimate the relative frequency for each index, and create heat maps based on Pearson’s and Spearman’s rank-order correlation analyses. In addition, it can also render 3D plots based on both yield performances and each index to separate entry genotypes into Fernandez’s groups (A, B, C and D), and performs principal component analysis. The accuracy of the results calculated from our software was tested using two different datasets obtained from previous experiments testing the salinity and drought stress in wheat genotypes, respectively. Conclusion: iPASTIC can be widely used in agronomy and plant breeding programs as a user-friendly interface for agronomists and breeders dealing with large volume data. The software is available at https://mohsenyousefian.com/ipastic/.
Alireza Pour-Aboughadareh
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Information flow diagram for the iPASTIC software.
 
Alireza Pour-Aboughadareh
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Development an online toolkit to calculate plant abiotic stress indices including the tolerance index (TOL), relative stress index (RSI), mean productivity (MP), harmonic mean (HM), yield stability index (YSI), geometric mean productivity (GMP), stress susceptibility index (SSI), stress tolerance index (STI), and yield index (YI).