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Karim Al-Jashamy
added 2 research items
The peripheral nerve injuries (PNI) are due to stretching and laceration. Therefore, the epineural repair technique is currently accepted as a standard method for peripheral nerve repair. Sixty adult male (60) rabbits were divided equally into perineural nerve sutures (ENS) as control group, omental pedicle transposition (OMPT) and bone marrow stromal cells implantation (BMSCs) as the treatment groups. The aim of this study was to investigate the effect and compare of OMPT and BMSC on the regeneration of sciatic nerve neurotmesis in rabbits. The OMPT and BMSc groups were significant improvement (p0.05) of the motor and sensory functions compared to the ENS. Histopathological, morphometric criteria, including the number of myelinated nerve fibers, nerve fiber diameter, axon diameter, myelin sheath thickness, g ratio and relative gastrocnemius muscle weight examinations showed that the OMPT group had the best regeneration and functional recovery compared to BMSCs and ENS.
Proteomics can widely be used as one of the tool for protein level analysis which has largely become a necessity because the study of genes by genomics might not adequately predict the structural dynamics of proteins. There is, however, a strong and synergistic relationship between proteomics and genomics as the two disciplines investigate the molecular organization of the cell at the complementary levels and increases the effectiveness of each other. In proteomics, the most promising technique with sufficient resolving power is two-dimensional gel electrophoresis (2DE) that provides a unique platform for the simultaneous separation of proteins in a complex mixture. However, the performance of optimization techniques in proteomics research using 2DE Manuscript: Journal of Biomedical Science 2 has its own limitations. In order to overcome these limitations, herein we have aimed for reviewing the application of 2DE proteomics in cancer research and in precise identification of specific and sensitive tumor markers over a period of time.
Hameed al-Timmemi
added 4 research items
Transdifferentiation is a process whereby a cell type committed to and progressing along a specifi c developmental lineage switches into another cell types. The objective of this study was to assess whether rabbit mesenchymal stem cells (rMSCs) precommitted to give mesenchymal cell lineage transdifferentiate in response to inductive extracellular cues to expand adult MSCs. Bone mesenchymal stem cells (BMSCs) obtained from ilium of adult male rabbit comprised heterogeneous groups of cells after seeding and growing in culture plates. After initial plating, the adherent cells exhibited small rounded, spindle-shaped and exhibited fi broblast-like morphology in reaching confl uence. Rabbit BMSCs differentiated into adipocytes and osteocyte as a accumulation of intracellular lipid droplets and calcium deposition throughout the culture after 21 days.
The PKH26 is a fluorescent lipophilic dyes used for the study of Asymmetric cell Divisions (ASDs) and efficiently purifies the stem cell fraction. The aim of this study was to explore the neurobiological characteristics in vitro and in vivo and tracking fate of the transplanted rabbit Bone Marrow-Mesenchymal Stem Cells (rBM-MSCs). A fluorescent microscope was used to determine the changes in cell size, fluorescence intensity during tissue culture, track cell divisions and the distribution of PKH26 dye between daughter cells. The results showed the identification of ASDs based on fluorescence intensity of the PKH26 dye was distributed equally between daughter cells at each division in vitro. The labeling BMSCs with PKH26 showed within the wall of the neurons in the dorsal root ganglia in vivo. Labeled BMSCs which are fibroblastic-like cells in P4 showed oval shaped and less density than P2. Direct examine of the labeled BMSCs in the cryosections at 16 weeks post operation showed the BMSCs were differentiated and appeared as like Schwann cells in an anastomosed sciatic nerve in the Local Treated Group (LTG). In the Systemic Treated Group (STG) sections, the labeled BMSCs were migrated to the anastomosed sciatic nerve, ipsilateral lumber dorsal root ganglia resembling glial and stellate cells and some of the labeled cells migrated to the anterior horn of spinal cord (motor neuron). In conclusion, the biological behaviors of BMSCs in vitro and in vivo showed highly mitosis at P2, activated fibroblast-like cells, differentiated to functional myelinating Schwann-like cells in LTG. The BMSCs in STG migrated and engrafted at the dorsal root ganglia as a neuron and glial cell, glial cells and satellite in the spinal cord.
The peripheral nervous system has the ability to regenerate after injury. Peripheral nerve injuries are caused by penetrating injury, crush, traction and ischemia compression. However, the availability of various nerve coaptation and other techniques for the attainment of functional nerve regeneration is still inadequate. The objective of this study was to compare the effectiveness of bone marrow stromal cells (BMSCs) implantation and epineural nerve suture on peripheral nerve regeneration in a rabbit model. Ten male New Zealand white rabbits were divided into two groups. In the primary epineural repair group (control group), the left sciatic nerve was skeletonized from the sciatic notch to the point of bifurcation, with the nerve been transected at the mid-shaft of the femoral bone and repaired with six epineural sutures. In the treated group, the epineural repaired nerve was implanted with BMSCs in the proximal and distal segments of the transected sciatic nerve. Assessment of the nerve regeneration was based on functional (motor and sensory), histological and morphometric criteria, including the number of myelinated nerve fibers, nerve fiber diameter, axon diameter, myelin sheath thickness, g ratio and relative gastrocnemius muscle weight. The results
Hameed al-Timmemi
added 2 research items
The peripheral nervous system is able to regenerate after injury. Etiologies of injuries include penetrating injury, crush, traction, and ischemia compression. However, the presence of various nerve injury treatments such as coaptation and another technique to attain functional nerve regeneration are still inadequate. This study aimed to compare the effectiveness of omental pedicle transposition and epineural nerve suture on peripheral nerve regeneration in a rabbit model. Ten male New Zealand white rabbits were divided into two groups. In the primary epineural repair group or control group (CG), the left sciatic nerve was skeletonized from the sciatic notch till the point of bifurcation. The nerve was transected at the mid shaft of the femoral bone and repaired with six epineural sutures. In the treatment group (TG), the epineural repaired sciatic nerve was wrapped with omental pedicle around the site of coaptation. Assessment of the nerve regeneration was based on functional (motor and sensory), histological, morphometric criteria and relative gastrocnemius muscle weight. The results of the examination show that the treated group had better regeneration and functional recovery. Keywords: Omental pedicle, regeneration, hispathological, morphometric, sciatic nerve
The role of topical wrapped of omental pedicle in preventing postoperative perineural fibrosis was examined by gross anatomical dissection and histological analysis in rabbits. The aim of study was to evaluate the role of omental pedicle in preventing postoperative perineural fibrosis of injured sciatic nerve in rabbits. The left sciatic nerve was exposed and transected into 12 adult male New Zealand white rabbits. The animals were divided into two groups six animals each, which named epineural nerve sutures group (ENS) as a positive control that the transected sciatic nerves was coaptation using epineural nerve sutures, whereas the omental pedicle wrapped around coaptated nerve in the omental pedicle transposition group (OMPT). The right sciatic nerves (non-operated) were collected from three animals of each group as a negative control group. Sixteen weeks post operation (PO), the operation site was evaluated by blinded surgical dissection. The results showed the there was graded adhesions of perineural into surrounded tissues using a numerical grading scheme. Morphometric analysis of distal segments of OMPT was statistically significant at P<0.05 difference compared with ENS group. Ultrastructural study showed OMPT group less collagen fibers proliferation than ENS group. It was concluded that OMPT is effective in decreasing epineural scar formation. of topical wrapping of omental transposition around transected sciatic nerve prevents epineural scar formation in rabbits.