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Tuberculosis, Sarcoidosis & Imunology of chronic diseases

Goal: Assessing new approaches on Tuberculosis, Pulmonary sarcoidosis, Lung cancer and related manifestations. Also searching for new therapies and regenerative medicine methods.

Date: 1 January 2017 - 22 April 2019

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Kimia Taghavi
added a research item
Contents Preparation of Primers Multiplex -PCR: Rifampicin Resistance (rpoB 516,526,531) (rpoB507, 518,533) (rpoB511, 513,522) Multiplex- PCR: Isoniazid Resistance (KatGOF, KatG5R, inhAp15, inhAPF2) Nested allele Specific PCR: Ethambutol Resistance (emb1 & emb 2) (emb306A & emb306B) PCR-RFLP: Pyrazinamide Resistance (PncA & Pnc B) PCR-RFLP: Kanamycin and Amikacin Resistance (PRS1539 &PRS1096) PCR-SSCP: Ciprofloxacin and Ofloxacin Resistance (GYR-A) PCR-SSCP: Novobiocin and Clorobiocin Resistance (GYR-B) PCR-ELISA: Isoniazid Resistance PCR-ELISA: Rifampicin Resistance References Susceptibility Testing Multiplex PCR PCR SSCP and PCR ELISA Mycobacteriology Research Center (MRC) Parissa Farnia, Kimia Taghavi, Shima Seif, Maryam Sheikholslami, Mohammad Varahram, Mohammad Reza Masjedi, Ali Akbar Velayati 2168099; Tehran: National Research Institute of Tuberculosis and Lung Diseases; 2010 Protocol (No: 2)
Kimia Taghavi
added 4 research items
Aim: The sarcoidosis as a multi-organ disease is characterized by noncaseating granuloma comprised of Thelper/ inducer (CD4+) lymphocytes and scant cytotoxic (CD8+) T-lymphocytes. This is the first report from Iran, evaluating the CD4: CD8 ratio capability in differentiating sarcoidosis from other interstitial lung diseases (ILDs) on a large cohort. Methodology: Fifty pulmonary sarcoidosis patients and 50 non-sarcoidosis interstitial lung diseases (nsILDs) patients were included in the current study. Bronchoalveolar Lavage (BAL) was performed using flexible fiberoptic bronchoscopy and flow cytometer. Results: Non-sarcoidosis group was established by 50 components that were classified into eight subgroups. As expected, the CD4/CD8 ratio was significantly higher in sarcoidosis than in non-sarcoidosis interstitial lung diseases (p < 0.001). The best cut off point in distinguishing sarcoidosis from other interstitial lung diseases was 1.1 with the sensitivity of 92% and specificity of 80%. Conclusions: Performing the safe and rapid method of bronchoalveolar Lavage in first step of diagnosing of sarcoidosis, confirms the disease in 92% of cases (current study sensitivity). Hence, only a few patients require undergoing an invasive procedure. Bronchoalveolar Lavage flow cytometry also supplies an appropriate diagnostic adjunct for discriminating sarcoidosis and non-sarcoidosis interstitial lung diseases. Keywords: sarcoidosis, Bronchoalveolar lavage, lymphocyte, CD4+:CD8+ ratio References 1. Bakhshayesh-Karam M, Tabarsi P, Mirsaiedi SM, Amiri MV, Zahirifard S, Mansoori SD, Masjedi MR, Velayati AA. Radiographic manifestations of Tuberculosis in HIV positive patients: Correlation with CD4+ T-cell count. International journal of mycobacteriology. 2016 Dec 31;5:S244-5. 2. Hunninghake GW, Costabel U, Ando M, Baughman R, Cordier JF, du Bois R, et al. ATS/ERS/WASOG statement on sarcoidosis. American Thoracic Society/European Respiratory Society/World Association of Sarcoidosis and other Granulomatous Disorders. Sarcoidosis Vasc Diffuse Lung Dis: official journal of WASOG. 1999;16(2):149-73. Epub 1999/11/24. 3. Eriksson M, Bennet R. What can we learn from the past?—A pediatrician’s view. International journal of mycobacteriology. 2016 Dec 31;5:S6. 4. Petruccioli E, Chiacchio T, Pepponi I, Vanini V, Urso R, Cuzzi G, Barcellini L, Palmieri F, Cirillo DM, Ippolito G, Goletti D. Characterization of the CD4 and CD8 T-cell response in the QuantiFERONTB Gold Plus kit. International journal of mycobacteriology. 2016 Dec 31;5:S25-6. 12
INTRODUCTION: Fatigue is one of the common symptoms of sarcoidosis, which occurs in about 50-70% of patients. AIM: Considering that there are no valid Iranian questionnaires for evaluating fatigue in sarcoidosis, in the present study, for the first time, we translated Fatigue Questionnaire into Persean and evaluated its validity and reliability among Iranian patients with sarcoidosis. MATERIAL AND METHODS: In methodological research, English version of Fatigue assessment scale (FAS) 10 items questionnaire which is designed to assess physical or mental fatigue in chronic disease patients, was translated into Persian and back-translated into English. Its validity and reliability were studied on the one hundred and thirteen confirmed sarcoidosis patients are referring to respiratory referral hospital of Iran. Reliability analysis was performed by estimation of Cronbach`s alpha test. RESULTS: According to the cut-off point of 22.84 (74%) of the studied patients were suffering from fatigue. The internal consistency calculation revealed that the alpha value of the physical fatigue and mental fatigue was 0.945 and 0.896, respectively. CONCLUSION: We concluded that the existence of questions number 4 and 10 in the questionnaire reduces the continuity of the questions, and therefore we suggest applying the FAS questionnaire without the two questions 4 and 10. This study showed that FAS questionnaire was very practical and can routinely be applied to assess the fatigue scale in sarcoidosis patients.
Authors: Atefeh Abedini , Mehdi Ramezanpour, Esmaeil Mortaz, Hamidreza Jamaati, Kimia Taghavi, Fatemeh Razavi, Arda Kiani Background: Anthracosis is the black pigmentation of the bronchial mucosa, caused by the deposition of carbon particles, silica, quartz, etc., in the mucosa, sub mucosa, and inside the macrophages, and in some cases, associated with pulmonary tuberculosis (TB). Aims and Objectives: The aim of the present study is to compare the serum and BAL fluid levels of CD4/CD8 ratio and IL-8, as potential diagnosis and prognosis biomarkers or anthracosis. Methods: 30 anthracosis patients, were included in the study. BAL sample and blood samples were collected, and sent to the Immunology laboratory for analysis. Patients with no anthracosis on bronchoscopy, and suspected to have TB, were included in the control group. In addition, BAL samples were used for BK-PCR. Results: 30 cases and 30 controls were included in the study. There were no significant differences in IL-8 of patients with anthracosis, compared to the control group. There were significant differences of CD4/CD8 ratio in BAL fluid, between anthracosis patients and the control group (1.01±0.77 vs. 2.41±3.50; p= 0.04), and significant differences were observed in serum level between two groups (p=0.02). Pulmonary TB was confirmed in 88.9% of patients with anthracosis, which was significantly higher than the control group. Conclusions: These results suggest that changes in CD4/C8 levels in serum and BAL fluid may play an important role in the diagnosis or prognosis of anthracosis. In addition, due to the strong association of anthracosis and pulmonary tuberculosis, TB should be considered in patients with anthracosis, which in turn can lead to the early diagnosis and treatment of the patients.
Atefeh Abedini
added a research item
ACTA FAC MED NAISS 2018;35(3):216-225 Original article UDC: 616.24-002.7-071 DOI: 10.2478/afmnai-2018-0023 S U M M A R Y Sarcoidosis is a multi-organ disease and is characterized by sarcoidal noncaseating granuloma comprised of T-helper/inducer (CD4+) lymphocytes and scant cytotoxic (CD8+) T-lymphocytes. CD4+:CD8+ T-cell elevated ratio is a characteristic diagnostic parameter for sarcoidosis. This is the first report from Iran evaluating the CD4:CD8 ratio capability in differentiating pulmonary sarcoidosis from other interstitial lung diseases (ILDs) on a large cohort. Fifty pulmonary sarcoidosis patients and 50 non-sarcoidosis interstitial lung diseases (nsILDs) patients were included in the current study. Bronchoalveolar lavage (BAL) was performed using flexible fiberoptic bronchoscopy and flow cytometer. Non-sarcoidosis group was established by 50 components that were classified into eight subgroups. Fifty-two per cent of sarcoidosis patients and 62% of non-sarcoidosis interstitial lung disease patients had normal spirometric results. The CD4/CD8 ratio was significantly higher in sarcoidosis than in non-sarcoidosis interstitial lung diseases (p < 0.001). The CD4/CD8 ratio was found to be > 3.5 in 33.3%, 2.5-3.5 in 7.1%, 1.5-2.5 in 20.2% and < 1.5 in 39.4% of the entire study population. The best cut off point was 1.1 with the sensitivity of 92% and https://publisher.medfak.ni.ac.rs/AFMN_1/2018/3-2018/abs%20english/CD4%20CD8%20RATIO%20A%20VALUABLE%20DIAGNOSTIC%20PARAMETER...htm
Kimia Taghavi
added a project goal
Assessing new approaches on Tuberculosis, Pulmonary sarcoidosis, Lung cancer and related manifestations. Also searching for new therapies and regenerative medicine methods.
 
Kimia Taghavi
added a research item
HOW TO CITE: Kiani A, Abedini A, Taghavi K, Razavi F, Seyedi SR, Fakharian A, Kharabian Masouleh S. Qualification of Two Chronic Obstructive Pulmonary Disease Persian Validated Questionnaires. InB64. COPD: LUNG FUNCTION, IMAGING AND PATHOPHYSIOLOGY 2018 May (pp. A3922-A3922). American Thoracic Society. American Journal of Respiratory and Critical Care Medicine 2018;197:A3922 Am J Respir Crit Care Med 2018; 197:A3922 Book B64. COPD: LUNG FUNCTION, IMAGING AND PATHOPHYSIOLOGY Pages A3922-A3922 Publisher American Thoracic Society Description RATIONALE: Chronic Obstructive Pulmonary Disease (COPD) Assessment Test (CAT) and St. George's Respiratory Questionnaire (SGRQ) are two validated health related quality of life questioners to use in patients with COPD. This study was aimed to investigate the correlation between these questioners in COPD patients. METHODS: The validated Farsi translation of CAT and SGRQ were applied to patients with clinically proven COPD from December 2014 to December 2015 at Iran referral respiratory hospital. The components of SGRQ score were calculated using excel according to SGRQ calculator. CAT and SGRQ questions were categorized into symptom, activity and impact, according to the meaning and concept of the questions. Data were analyzed by statistical software (SPSS 22) and the level of statistical significance was set at P< 0.05. RESULTS: In this study, 99 patients including … https://www.atsjournals.org/doi/abs/10.1164/ajrccm-conference.2018.197.1_MeetingAbstracts.A3922 https://scholar.google.co.in/scholar?hl=en&as_sdt=0%2C5&q=Qualification+of+Two+Chronic+Obstructive+Pulmonary+Disease+Persian+Validated+Questionnaires+&btnG=
Kimia Taghavi
added an update
Led by Dr. Arda Kiani and Dr. Atefeh Abedini, we have conducted a number of studies on Sarcoidosis, Tuberculosis and Anthracosis. Sarcoidosis research group is the reference registry center for sarcoidosis patients in Iran. Each year, the number of patients referred to our center increases and we are developing the registration of sarcoidosis patients in Iran. We currently have registered about 500 sarcoidosis patients and have three great resulted articles ahead which are all conducted for the first time in such in Iran. We would be happy to share our results with other researchers very soon.
 
Kimia Taghavi
added a research item
Background: Asthma treatment guidelines suggest the target of treatment ought to be optimal control. Asthma control test questionnaire alongside pulmonary function tests, is validated guideline for asthma control worldwide. Current study headed to evaluate correlations between asthma control and pulmonary function test results. Components and Techniques: Two hundred fifty five asthma respondents enrolled in the current study. Asthma control was estimated by asthma control test questionnaire and lung function test was assessed by spirometer. Statistical analysis was performed and results comparison was obtained. Outcome: One hundred thirty nine patients (54.7%) were classified in the controlled asthma group (asthma score obtained 20 or higher). Fifty seven patients (22.4%) and 58 patients (22.8%) were categorized as the partially and poorly controlled asthma groups respectively (15< asthma score <20, asthma score <15). Significant relationship was demonstrated between asthma control test and forced expiratory volume in one second pulmonary function parameters (P<0.001). A highly significant correlation was obtained between forced expiratory volume in one second and the four last questions of asthma control test (r=0.19, P value <0.001). Conclusion: Asthma control test questionnaire is considered to be a reliable test in everyday asthma treatment guide in Iran. Asthma score ≥ 20 is proposed to be adequate for detecting controlled asthma in Iran with no obligatory additional pulmonary functioning test. Keywords: Asthma (C08.127.108); Questionnaire (E05.318.308.980); Lung (A04.411); Prevalence (E05.318.308.985.525.750); Asthma control test