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Introduction: The search for lab innovation in newborn screening is a constant process. The implementation of new markers, the necessity to obtain a robust process, cost effectiveness while minimizing steps, reduce infrastructure adjustments, reduce investments in human resources, keep or improve quality in the results are considered essential points. The Brazilian industry, with the objective to offer better solutions for newborn screening labs has developed a new solution for quantification of Phenylalanine, Total Galactose and Leucine using a totally automated system and microplates with 384 wells. Materials and Methods : An automatic puncher was configured for the use of 384 wells, spots of 3mm in diameter, associated to a totally automated equipment ( Nimbus NeoLISA 384 ) and the kits NeoLISA PKU 384, NeoLISA GAL 384 and NeoLISA MSUD 384 to run in one batch/routine 1940 samples. A run with 1940 samples takes 7 hours. For the same number of samples using the 96 well in automated system the same process takes 12 hours. Samples from different concentrations of each analyte, external quality control material and internal controls have been used for comparison analysis and reproducibility. The results are directly compared to the 96 well format in a totally automated system. A demonstrative graphics and tables are used to present the data. Results : Excellent reproducibility was observed between 384 well analysis and 96 well analysis. While a routine workflow with 1940 samples is rare, since most labs run 500-1000 samples a day, demonstrating the capacity of the equipment, the reduction of time process and the possibility to run simultaneously the 3 products in the same run is important. Conclusion : The use of 384 wells associated with a totally automated equipment, Nimbus NeoLISA 384 and kits NeoLISA PKU, GAL and MSUD 384 are considered an innovation for newborn screening labs in the quantification of Phenylalanine, Total Galactose and Leucine.