Project

NMRSIGN

Goal: Gas-sensing proteins play an important role in mediating biological events. Soluble guanylate cyclase is activated when nitric oxide (NO) binds to heme Fe of sGC, and then stimulates cyclization of guanosine 5-triphosphate to the second messenger cyclic guanosine 3,5-monophosphate, which in turn has a direct role in the control of a variety of physiological processes in several signal transduction pathways. Disruptions in the NO/sGC/cGMP signalling pathway have been linked to a variety of diseases including congestive heart failure, stroke, hypertension and neurodegeneration. In this project, we will derive novel molecular insight into the heme-containing sensor domain of sGC that is the primary receptor for NO and therefore plays a significant role in NO-signalling by employing the power of solution NMR spectroscopy and enhanced conformational sampling computational approaches.

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Project log

Georgios Dalkas
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Gas-sensing proteins play an important role in mediating biological events. Soluble guanylate cyclase is activated when nitric oxide (NO) binds to heme Fe of sGC, and then stimulates cyclization of guanosine 5-triphosphate to the second messenger cyclic guanosine 3,5-monophosphate, which in turn has a direct role in the control of a variety of physiological processes in several signal transduction pathways. Disruptions in the NO/sGC/cGMP signalling pathway have been linked to a variety of diseases including congestive heart failure, stroke, hypertension and neurodegeneration. In this project, we will derive novel molecular insight into the heme-containing sensor domain of sGC that is the primary receptor for NO and therefore plays a significant role in NO-signalling by employing the power of solution NMR spectroscopy and enhanced conformational sampling computational approaches.