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Taisir Abdulelah Kadhim
added a research item
PATHOGENIC BACTERIA Virulence and Diseases By Group of Iraqi Academics
Taisir Abdulelah Kadhim
added a research item
Objective: The aim of the present study was to evaluate the presence of Aggregatibacter actinomycetemcomitans (A. actinomycetemcomitans) and Porphyromonas gingivalis (P. gingivalis) in healthy and different stage of gingivitis for children aged between 9 and 12 years using Polymerase Chain Reaction (PCR) and to compare their presence in children in their healthy, mild and severe gingivitis. Method: Subgingival plaque samples were collected from 94 children and were grouped as Group A-control (healthy), Group B-mild gingivitis, Group C moderate gingivitis, and Group D sever gingivitis and were subjected to PCR assay. Results: All data were analyzed by using SPSS program (version 21 for windows 7) chi-square test at p≤0.05. A. actinomycetemcomitans was detected in 0 (0%), 4 (13.4%), 37 (92.5%) and 12 (100%) whereas P. gingivalis was detected in 0 (0%), 0 (0%), 6 (15%) and 0 (0%) samples in group A, B, C and D respectively Conclusion: There is nor A. actinomycetemcomitans or Porphyromonas gingivalis found in group A (healthy moth children). Both the microorganisms were present in group C. In group B and D only A. actinomycetemcomitans was present.
Shayma Abdullah
added 2 research items
Food safety is very necessary for the general health of the consumer and protection from disease, as well as the importance of economic sustain ability of the agricultural food sector. The consumer wants to make sure that the food is safe and that it is not contaminated with bacteria. Therefore, these tests must be performed to detect bacteria and ensure that food safety. 120 samples was collected from frozen chicken cans from the markets of Al-Muthanna province, using the sterile plastic bags and after isolation the results showed that (55) sample contain bacteria Pseudomonas aeruginosa from the total samples above. The results of bacterial culture were positive in 45 samples whereas the results of direct molecular identification were positive in 55 samples. Selective media used for bacteria identification such as MacConkey Agar, Methyle red which has been given the proportion of isolation (15% and 85% respectively) also some isolates showed their ability to liquefy gelatin (65%) and fermented sugar lactose (100%). however biochemical testing also used for all bacterial isolates for confirm diagnoses. Polymerase chain reaction technique used for detection bacteria by specific primers sequence the result appear 45% of total sample contamination by P. aeruginosa. The genome of P. aeruginosa is relatively large so encodes a large proportion of regulatory enzymes important for metabolism and efflux of organic compounds. This enhanced coding capability genome allows for great metabolic versatility and high adaptability to environmental changes, According our result we recommend food factories to use sterilized materials more accurately and effectively to store frozen chicken cans.
Molecular detection of pseudomonos Aeruginosa