CherrySeRB - ‘Genetic potential of Serbian autochthonous cherry genotypes for temperature-adaptable reproductive behaviour and nutraceutical value’
Goal: CherrySeRB is basically oriented towards the field of agro-biodiversity, aiming to add value to unexplored Serbian and Balkan fruit genetic resources through new protocols and approaches for their genotypic and phenotypic characterisation. Two fruit species of worldwide importance are targeted diploid sweet cherry and tetraploid sour cherry. The main idea of CherrySeRB is connection of the production challenges arising from climate change and demands for quality and healthy fruits, and the richness of Serbian cherry germplasm that is still unused enough. Characterisation of the indigenous genotype potential for desirable reproductive properties and defining ʻgood reproductive behaviour cherry modelʼ that can face warmer temperature conditions during the flowering are in focus. CherrySeRB will also provide S-genotyping and the choice of suitable pollenisers that maximize fertilization efficacy for landraces, as well as their nutritional/phytochemical profiles that will enable obtaining of new high-added value nutraceutical products.Organized around four pillars that include six WPs, CherrySeRB combines interdisciplinary modern methodologies – molecular, for identification of S-haplotypes in definition of GSI-status (DNA diagnostic strategy); biological, for revealing suitable polleniser(s) (assessment of the pollen tube growth in vivo by fluorescence microscopy; the fertilization efficacy parameters); histological, for determining ovary function after exposing to different flowering temperatures (cyto-embryological and histochemical testing); nutritional, for characterisation of fruit quality and health-promoting compounds (texture and colorimetric analysis, HPLC, UV-Vis, etc.); genotype identification (S-genotyping; pollen grain characterisation by SEM); statistical (models in reaching new scientific conclusions). Data, protocols and novel cherry material will be made available to breeders, biotechnology scientists and cherry-sector stakeholders. Representing novelty in breeding approach that can be applicable to other fruit species, the ultimate goal of CherrySeRB is to foster the use of Serbian cherry germplasm as the material adapted to climate change in order to contribute to sustainable fruit production and ensure the availability of healthy food for the future.
Date: 25 January 2022 - 24 January 2025
- N. Milosevic's Lab