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Avipattikara churna is an Ayurvedic medicine, it is a mixture of 14 herbal and mineral materials in powder form. It is used in Ayurvedic treatment of agnimandya (digestive impairment), malabandha (constipation), amlapitta (dyspepsia), arsa (haemorrhoids), mutrabandha (retention of urine), prameha (urinary disorders) and piles. Avipattikara churna is a totally polyherbal classical medicine without any side effects. In this study attempts to evaluate the main parameters of drug standardization and HPTLC finger print profile of avipattikara churna based on organoleptic evaluation,physical characteristics, physicochemical studies like pH, total ash, acid insoluble ash, loss on drying at 105°C, extractive values etc.and microbial load were carried out as per the API, IP, WHO and AOAC guidelines to substantiate the adulteration for quality control of herbal drugs. The set parameters were found to be sufficient to evaluate the churna formulation and can be used as reference standards for the quality control/quality assurance for Ayurvedic formulations.
The present study was aimed to evaluate the purity of the ingredients through the assay of marker compound to ensure the quality of the prepared poly herbal formulation in the course of standardization. It was carried out by isolation of piperine from poly herbal formulation Trikakuchurna besides one of its ingredient namely Piper nigrum; quantitative evaluation of Piperine through densitometry, using HPTLC for poly herbal formulation of in-house prepared and market purchased samples besides ingredients. The isolated compound was identified by UV, H1 NMR, and LC-MS. Thus the presence of marker not only identified in the formulations but also physically found with the isolated marker compound to ensure the quality of the finished product.
Triphala churna is well known Ayurvedic formulation in official Ayurvedic Formulary of India, It is used for immune system stimulation, improvement of digestion, relief of constipation, gastrointestinal tract cleansing, relief of gas, treatment of diabetes, eye disease, anaemia, jaundice, asthma, fever, chronic ulcers etc. Standardization of herbal formulation is essential in order to assess the quality, purity, safety and efficacy of the drug. In this, study attempts to evaluate the qualitative estimation of bio active compounds Gallic acid and Ellagic acid by HPTLC and quantification of Gallic acid by HPTLC and HPLC Chromatographic methods. The method employed in current study resulted in good peak shape of Gallic acid. The Rf value of Ellagic acid 0.47 and Gallic acid is 0.56 in all the samples and reference standard was found comparable under UV light at 254 nm and 366 nm. The percentage of free Gallic acid is prominent in Emblica officinalis and lower in Terminalia chebula of Triphala churna ingredients.
Anxiety symptoms are common in medically ill patients 5 to 20% of medical in patients and 4 to 14% of general medical out patients suffer from anxiety disorders, It may occur as a manifestation of a primary psychiatric disorder or secondarily to either the medical illness or the medications prescribed for treatment (Harrison). Chittodvega a minor psychiatric disorder has been mentioned by Charaka (Charak Vi. 6/5), which is produced due to vitiation of Raja and Tama. In addition Prana, Udana, Vyana Vayu; Sadhaka Pitta; and Tarpaka Kapha are also provocated factors in it. Etymology of Chittodvega i.e. anxious status of mind is similar to anxiety, somatic manifestation are also same in both the condition. Shirodhara therapy has been found as safe and effective treatment modalities in anxiety, irritability, inability to relax, lack of concentration, disturbed sleep, loss of memory, palpitation, headache, dryness of mouth, upset stomach and restlessness. It is having tranquilizing and anxiolytic effect resulting into a kind of relaxation response.
The present study was carried out for evaluation of TLC fingerprint profiles of volatile oils/essential oils of the each ingredient of the polyherbal formulation of Trikatu churna and comparison of the volatile oil of the finished product or formulation as such. Essential oils/volatile oil contents are characteristic to the ingredients or raw botanicals to identify them easily. In the raw botanicals, where ever its presence is possible and its verification through the TLC/HPTLC fingerprint profile is the one of the best ways to identify and evaluate the quality of the finished formulation in the course of development of a standardization protocol as a quality control tool.
Sitopaladi churna is well known ayurvedic formulation is official in Ayurvedic Formulary of India, traditionally used for asthma, cough and cold, tuberculosis, chest pain, chronic rhinitis/sinusitis, coryza and other respiratory disorders. It is used as an anti-tussive, analgesic and antipyretic. It is observed that the consistency and content varies from one manufacturer to another which affects therapeutic activity of the formulations. Hence, it is needed to develop a protocol for the evaluation of herbal drugs. In this study attempts to evaluate the main parameters of drug standardization like Organoleptic characteristics, phamacognostic study, extractive values, ash values, physical characteristics, loss on drying and TLC. This study on Sitopaladi churna was precise, reproducible and may be considered as a protocol for its evaluation and establishment.
Plants, source of various bioactive compounds have played crucial role in development of several clinically useful anticancer agents' viz., vincristin, vinblastin, camptothecin derivatives and taxol. Combretastatin, a potent anticancer molecule was first isolated from plant. In this continuation a series of combretastatin analogues has been synthesized on the basis of our butterfly model with two wings as aryl groups and connecting carbon chain as the body. In present study we have synthesized combretastatin analogues of different substitutions, showed potent anticancer activity against different cell lines in different fashion according to substitution. Compound 6 showed marked anticancer activity against colo-205 cell lines (colon cancer).
Standardization of Ayurvedic formulations is essential in order to assess the quality, purity, safety and efficacy of drugs based on the amounts of their active principles. The present research work is an attempt to standardize "Srngyadi Churna" an ayurvedic polyherbal formulation used in the treatment of cough, asthma and fever. The formulation was prepared in institute pharmacy as per Ayurvedic formulary of India, Part-I guide lines and attempts to evaluate the organoleptic characters, phamacognostic study and physicochemical parameters like pH, Loss on drying at 105°C, Water soluble extract, Alcohol soluble extract, Total Ash, Acid insoluble ash and TLC. The study revealed specific identities for crude drug taken which will be useful in identification and control to adulterations of the drugs.
Aim: Trikatu churna is an important and unique polyherbal formulation used for various therapeutic indications. The World Health Organization (WHO) has emphasized the need to ensure the quality of medicinal plant products and herbal formulations by using any modern controlled techniques. High-performance thin-layer chromatography (HPTLC) is a frequently used technique to develop the chromatographic fingerprints. Therefore, the present study was carried out for quality evaluation of Trikatu churna through the development of standardization protocol with comparative HPTLC fingerprint profiles of successive extracts besides routine analysis. Materials and methods: Successive extracts of various solvents of polarity in increasing order through soxhlet apparatus for Trikatu churna and its authenticated raw materials were prepared; comparative chromatographic fingerprint profiles developed along with the evaluation of qualitative and quantitative tests parameters have been carried out by using the Ultraviolet (UV)-visible spectrophotometer, flame photometer, and atomic absorption spectrophotometer. Results: The following test parameters physicochemical parameters, evaluation of qualitative, quantitative phytochemi-cals such as total tannins, sugars and phenols, micronutrients such as sodium (Na), potassium (K); safety parameters such as microbial load, heavy metals and aflatoxins; comparative TLC and HPTLC fingerprint profiles of successive extracts of ingredients in increasing the polarity order of the solvents (n-hexane to alcohol) with respect to that of consecutive extracts of the final polyherbal formulation have been carried out for the quality evaluation. Conclusion: Trikatu churna was evaluated for its identity and purity by the systematic analysis of the above parameters for raw botanical ingredients and formulation in the course of development of standardization protocol for quality control.
Hiptage benghalensis (L) Kurz belongs to the family Malphigiaceae. The plant has strong therapeutic potential thus occasionally cultivated for medicinal purposes in several traditional medicines to cure various diseases. This plant has been known to possess antibacterial, antifungal, antidiabetic, anti-inflammatory, anticancerous, antimutagenic and hepatoprotective activity. According to Ayurveda, Hiptage benghalensis is cooling, vulnerary, astringent, expectorant, cardiotonic, anti-inflammatory, insecticidal, wound healing and used in burning sensation of the body, wound, pruritus, foul ulcers, scabies, leprosy, skin diseases, cough, asthma, cardiac debility, rheumatism, hyperdipsia, obesity, intrinsic haemorrhage etc. The presented review summarizes the information concerning the botany, ethno pharmacology, phytochemistry and biological activity of this plant.
Research J. Pharm. and Tech. 11(9): Septembert 2018 1 ISSN 0974-3618 (Print) www.rjptonline.org 0974-360X (Online) RESEARCH ARTICLE A Review on Different Sources of Piper nigrum L. Adulterants Rinky Thakur1 , Ajay Kumar Meena2 , Amit Kumar Dixit1 , Suma Joshi4 1Regional Ayurveda Research Institute for Metabolic Disorder, CCRAS, Ministry of AYUSH, Bengaluru, 2Captain Srinivasa Murthy Regional Ayurveda Drug Development Institute, CCRAS, Ministry of AYUSH, Arumbakkam, Chennai, 3 KAHER’s Shri BMK Ayurveda Mahavidalaya, Belagavi. *Corresponding Author E-mail: email@example.com ABSTRACT: Medicinal plants constitute an effective source of traditional Ayurvedic system, with increasing believe and demands in Ayurvedic medicines, the adulteration in medicinal plants has also became a burning problem in the pharmaceutical industry. Adulterations is a practice of substituting original or genuine drugs with similar looking drugs which have different chemical constituents and therapeutic properties, thus producing useless or harmful substance with intention of enhancement of profit. It may be due to wrong identification, deliberate mixture of other drugs, lack of knowledge about authentic plants, non availability, similarity in morphology, careless collection and any other unknown reasons. Proper identification of medicinal herbs/plants play vital role in producing authentic genuine Ayurvedic drugs. The identification of important drug is must, if someone fails to identify the genuine drug the Ayurvedic formulation goes wrong due to adulteration; efficacy and potency of medicines goes down and also leads to variety of complications, adverse effects from mild to severe life threatening conditions. Correct identification and usage of genuine drug is very important to reduce the adverse effect and maximizing consumer’s safety. In this paper different adulterated forms of Maricha (Piper nigrum L.), such as Eranda karkati (Carica papaya L.) Sandhyaaraaga (Mirabilis jalapa L.), Chavika/Kankola (Piper cubeba L. f.), Shatavari (Asparagus racemosus) and Nirgundi (Vitex Negundo) have been discussed in order to differentiate genuine drugs and adulterants through diagnostic characteristics. These could be used as an adulterant due to similar morphology, non-availability of requisite material and improper identification of genuine drugs. KEYWORDS: Adulterant, Piper nigrum L., Carica papaya, Ayurvedic, Plants, Drugs
Triphala churna is well known Ayurvedic formulation in official Ayurvedic Formulary of India, It is used for immune system stimulation, improvement of digestion, relief of constipation, gastrointestinal tract cleansing, relief of gas, treatment of diabetes, eye disease, anaemia, jaundice, asthma, fever, chronic ulcers etc. Standardization of herbal formulation is essential in order to assess the quality, purity, safety and efficacy of the drug. In this, study attempts to evaluate the qualitative estimation of bio active compounds Gallic acid and Ellagic acid by HPTLC and quantification of Gallic acid by HPTLC and HPLC Chromatographic methods. The method employed in current study resulted in good peak shape of Gallic acid. The Rf value of Ellagic acid 0.47 and Gallic acid is 0.56 in all the samples and reference standard was found comparable under UV light at 254 nm and 366 nm. The percentage of free Gallic acid is prominent in Emblica officinalis and lower in Terminalia chebula of Triphala churna ingredients. KEYWORDS: Gallic acid, Ayurveda, Standardization, Chromatographic, HPLC and HPTLC. Cite: Ajay Kumar Meena, Ch. V. Narasimhaji, D. Velvizhi, Arjun Singh, P. Rekha, Vijay Kumar, R. Ilavarasan, Narayanam Srikanth, K. S. Dhiman. Determination of Gallic Acid in Ayurvedic Polyherbal Formulation Triphala churna and its ingredients by HPLC and HPTLC. Research J. Pharm. and Tech 2018; 11(8): 3243-3249.
The present article attempts to compare HPTLC and physico-chemical parameters of Cinnamomum zeylanicum and Cinnamomum cassia belonging to common family Lauraceae and genus Cinnamomum. Each of them is considered to have huge medicinal value in Ayurveda, Siddha and Unani traditional medicines. Since ancient times, these drugs are used according to their medical value. Investigation of such traditionally used medicinal plants is thus valuable on two levels, firstly, as a source of potential chemo-therapeutic drugs and secondly, as a measure of safety for the continued use of medicinal plants. The present paper attempts to evaluate the physicochemical parameters like water soluble extract, alcohol soluble extract, total ash, acid insoluble ash, TLC/HPTLC and preliminary phytochemical analysis. The study revealed specific identities for crude drug taken which will be useful in identification and control of adulterations and/or substitution of the raw drug.
Ayurveda and Siddha provides number of Single, Compound herbal/herbo-mineral Rasayana drugs contributing unique Rasayana therapy having diversified actions on different systems of the body. Siddha experts indicate that Siddha drugs have been proved to be useful in controlling venereal diseases including diseases closely resembling HIV clinically. The drugs like Raca Kanti Meluku, Amukkarac curanam and Nellikkai Ilakam in combination were useful in HIV patients. Considering their therapeutic importance, the research studies have been conducted & reported in present Technical Report. DR-7 DR-8 CCRA
Introduction: AYUSH 82 powder is an Ayurvedic antidiabetic formulation developed by the Central Council for Research in Ayurvedic Sciences (CCRAS), Ministry of AYUSH, Govern ment of India. The antidiabetic potential of AYUSH 82 powder along with its two new dosage forms-AYUSH 82 mixture extract and AYUSH 82 compound extract-has been established to be mediated via α-amylase inhibitory property, insulin-dependent glucose uptake in skeletal muscle cell line (C2C12 myotubes).
Background: Samasharkara Churna, a polyherbal Ayurvedic formulation, is prescribed for treating various conditions such as asthma and cough. Literature review suggested that characterization parameters of Samasharkara Churna are not reported. Objective: To report characteristic parameters of Samasharkara Churna to conform its identity, quality and purity. Materials and methods: Samasharkara Churna was evaluated for pharmacognostic, physicochemical, microbiological, and chromatographic parameters. The chromatographic analysis was able to showed presence of all ingredients in Samasharkara Churna. Conclusion: The characterization parameters presented in this paper may serve as standard reference for the quality control analysis of Samasharkara Churna.
Objective: Standardization of any herbal formulation is essential in order to assess the quality, purity, safety, and efficacy of drugs based on the analysis of their active properties. Testing of Ayurvedic preparations using scientific methodologies adds to quality and authenticity of the product. Methods: This article reports standardization parameters for a classical Ayurvedic formulation Panchasakara Churna. In this paper, the formulation was prepared as per Ayurvedic Formulary of India and was characterized by pharmacognostic, physical, physicochemical, phytochemical, toxicological parameters as well as thin layer chromatography (TLC) profiling using standard methodologies. Results: This experimental work provided diagnostic characteristics to identify and standardize the formulation Panchasakara Churna prepared using its official ingredients. Conclusion: Based on the present investigation results, a monograph on quality standards for Panchasakara Churna can be proposed for its batch-to-batch consistency. This document can also be utilised for rapid authentication fingerprints of this formulation using its TLC profiling.
The present communication attempts to evaluate the physicochemical and preliminary phytochemical studies on the roots of Glycyrrhiza glabra Linn Leguminosae family. Glycyrrhiza glabra is a hardly herb or undershurb of pea family, found in subtropical and warm temperate regions. It is up to four or five feet, Glycyrrhiza glabra is widely distributed in Mediterranean countries, South Europe, Asia Minor, Egypt, Turkistan, Iran and in India, it is reported to be cultivated in Baramulla, Srinagar, Jammu, Dehradun, Delhi and South India. The rhizomes are considered to possess an expectorant and carminative, flavouring agent, depressant, antimicrobial, hypolipidemic, antianthersclerotic, antiviral, hypotensive, heptoprotective, spasmolytic, antidiuretic, antimutagenic, antipyretic, anti-inflammatory. As there is no detailed standardisation work reported on rhizome, the physicochemical parameters, preliminary phytochemical constants, toxic heavy metals, pesticide residue, aflatoxin and microbial contamination analysis are carried out. The study revealed specific identities for the particular crude drug which will be useful in identification and control to adulterations of the raw drug.
Ayurvedic medicine, Pancasama Churna known to be effective mainly on gastrointestinal tract (GIT), has been standardized by following modern scientific quality control procedures both for the raw material and the finished product. Pancasama Churna was subjected to macro-microscopic, Physico-chemical, preliminary phytochemical, TLC and HPTLC to fix the quality standards of this drug. This study results a set of diagnostic characters essential for its standardisation. TLC and HPTLC fingerprinting were employed to fix standards. The values obtained after physicochemical parameters study showed that these values should be helpful to develop new pharmacopoeial standards. This will be helpful to overcome batch to batch variations in traditional preparation of Pancasama churna. The physicochemical constituents found to be present in raw material used for the preparation of Pancasama churna possibly facilitate the desirable therapeutic efficacy of the medicinal formulation. KEYWORDS
The herbal single drug Aftimoon is botanically called as Cuscuta reflexa Roxb. (whole plant). In the recent past tremendous attempts are being made to evaluate the scientific standards for Traditional System of Medicine, particularly in Unani System of Medicine. The Physician of Unani System of Medicine has considered this drug as one of the important single drugs and this drug has been used extensively in the preparation of Unani Compound Formulations such as Itrifal-e-Aftimoon, Itrifal-e-Ustukhudus, Itrifal-e-Deedan, Itrifal-e-Ghudadi, Itrifal-e-Mushil, Majoon-e-Ushba and Sufoof-e-Chobchini etc., This drug is therapeutically useful in the treatment of insanity, melancholia, melanous, epilepsy, numpness, paralysis, facial palsy, arthritis, worm infestation, jaundice and in the weakness of liver, stomach and spleen. As per the action the drug is very effective in cathartic to black bile and phlegm, demulcent, resolvent, carminative and antihelmintic. In order to authenticate and to develop its pharmacopoeial standards WHO guidelines were followed provided by CCRUM, New Delhi. For the Evaluation of Pharmacopoeial standards Pharmacognostical and Phyto-chemical parameters were adopted. The Thin Layer Chromatographic studies of petroleum ether (60 -80°), chloroform and alcohol extract was also carried out to ascertain the quality of this drug.
Standardization of herbal formulations is essential in order to assess the quality, purity, safety and efficacy of drugs based on the amounts of their active principles. The aim of the present work is to standardization of Trikatu churna. The churna makes this traditional drug more stable for long term storage and hence, easier to prepare. The Trikatu churna is a reputed drug mentioned in the ancient books of Ayurveda used for the treatment of fever, asthma, cold and cough, diabetes, nasal diseases, obesity, anorexia, digestive, respiratory system and normal urinary tract function. Trikatu churna was prepared as per Ayurvedic Formulary of India and attempts to evaluate the Organoleptic characters, phytochemical study, phamacognostic study and physicochemical parameters like pH, Loss on drying at 105°C, Water soluble extract, Alcohol soluble extract, Total Ash, Acid insoluble ash. The study revealed specific identities for crude drug taken which will be useful in identification and control to adulterations of the drugs.