Zsolt Szilagyi

Zsolt Szilagyi
University of Gothenburg | GU · Department of Medical Biochemistry and Cell Biology

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37
Publications
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514
Citations

Publications

Publications (37)
Article
The multiprotein Mediator complex is required for the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator contains the Cdk8 regulatory subcomplex, which directs periodic transcription and influences cell cycle progression in fission yeast. We here investigate the role of CycC, the cognate cyclin partner of Cdk8, in cel...
Article
Full-text available
Author summary Human mitochondria contain a small double-stranded DNA genome (mtDNA) of only 16,569 base pairs (bp) that encodes 13 essential subunits of the oxidative phosphorylation system. Depletion of mtDNA and different types of mtDNA mutations cause mitochondrial disease, and are also implicated in biological ageing. For almost half a century...
Data
Spearmans coefficient was calculated for H-strand (HS) and L-strand (LS) in HydEn-seq and 5´-End-seq libraries for mtDNA isolated from HeLa cells. (PDF)
Data
Summarized signal at HincII sites in mtDNA in L-strand (upper left panel), mtDNA in H-strand (lower left panel), nuclear DNA in top strand (upper right panel), nuclear DNA in bottom strand (lower right panel). (PDF)
Data
5´-End-seq and HydEn-seq results at OriH. (PDF)
Article
Full-text available
Cell proliferation is regulated by cyclin-dependent kinases (Cdks) and requires the periodic expression of particular gene clusters in different cell cycle phases. However, the interplay between the networks that generate these transcriptional oscillations and the core cell cycle machinery remains largely unexplored. In this work, we use a syntheti...
Article
Full-text available
Cdk8 is required for correct timing of mitotic progression in fission yeast. How the activity of Cdk8 is regulated is unclear, since the kinase is not activated by T-loop phosphorylation and its partner, CycC, does not oscillate. Cdk8 is, however, a component of the multiprotein Mediator complex, a conserved coregulator of eukaryotic transcription...
Article
Cyclin dependent kinase 8 (Cdk8) is a component of Mediator, an evolutionary conserved multiprotein complex that regulates RNA polymerase II-dependent transcription. Cdk8 has been implicated as a regulator of multiple steps in cell cycle progression. We here discuss recent advances in our understanding of Cdk8 function and a possible role for Media...
Article
Full-text available
At Schizosaccharomyces pombe centromeres, heterochromatin formation is required for de novo incorporation of the histone H3 variant CENP-ACnp1, which in turn directs kinetochore assembly and ultimately chromosome segregation during mitosis. Noncoding RNAs (ncRNAs) transcribed by RNA polymerase II (Pol II) directs heterochromatin formation through n...
Article
Full-text available
Temporal changes in transcription programs are coupled to control of cell growth and division. We here report that Mediator, a conserved coregulator of eukaryotic transcription, is part of a regulatory pathway that controls mitotic entry in fission yeast. The Mediator subunit cyclin-dependent kinase 8 (Cdk8) phosphorylates the forkhead 2 (Fkh2) pro...
Article
Full-text available
The multiprotein Mediator complex is an important regulator of RNA polymerase II-dependent genes in eukaryotic cells. In contrast to the situation in many other eukaryotes, the conserved Med15 protein is not a stable component of Mediator isolated from fission yeast. We here demonstrate that Med15 exists in a protein complex together with Hrp1, a C...
Article
The general transcription factor IID (TFIID) is required for initiation of RNA polymerase II-dependent transcription at many eukaryotic promoters. TFIID comprises the TATA-binding protein (TBP) and several conserved TBP-associated factors (TAFs). Recognition of the core promoter by TFIID assists assembly of the preinitiation complex. Using cryo-ele...
Article
Schizosaccharomyces cells divide by medial septation, followed by enzymatic degradation of parts of the septum (septum cleavage) to allow the sister cells to separate from each other. In a previous study we found that the cell separation mutant sep9-307 was defective in a gene that encodes a protein highly similar in sequence to the Saccharomyces c...
Article
Full-text available
Cell division is controlled by a complex network involving regulated transcription of genes and postranslational modification of proteins. The aim of this study is to demonstrate that the Mediator complex, a general regulator of transcription, is involved in the regulation of the second phase (cell separation) of cell division of the fission yeast...
Article
Full-text available
The fission yeast Mcs6-Mcs2-Pmh1 complex, homologous to metazoan Cdk7-cyclin H-Mat1, has dual functions in cell division and transcription: as a partially redundant cyclin-dependent kinase (CDK)-activating kinase (CAK) that phosphorylates the major cell cycle CDK, Cdc2, on Thr-167; and as the RNA polymerase (Pol) II carboxyl-terminal domain (CTD) k...
Article
The fork-head type transcription factors are a class of regulators that function in a broad spectrum of cellular and developmental processes in many species ranging from yeasts to human. Previous data on yeast fork-head genes suggested roles for these regulators in the control of cell division, sexual differentiation and development. The genome of...
Article
Full-text available
Morphologic and physiologic changes taking place in carbon-limited submerged cultures of Aspergillus nidulans ΔflbA and fadA G203R strains were studied. Loss-of-function mutation of the flbA gene resulted in an altered germination with unusually thick germination tubes, “fluffy” pellet morphology, as well as a reduced fragmentation rate of hyphae d...
Article
We have previously described the genetic analysis of eleven complementation groups (sep6–sep16) defined by Schizosaccharomyces pombe mutants that are defective in cell separation and sexual differentiation. Here we report on the cloning and characterisation of two members of this set, sep10 and sep11. Sequencing of the full-length sep10 revealed a...
Article
The dimorphic fission yeast Schizosaccharomyces japonicus has proved to be an excellent experimental model for the investigation of the eukaryotic cell. Here we show that it has a haplontic life cycle, in which the diploid phase is confined to the zygote. To make it amenable to genetic and molecular analysis, we generated genetic markers and cloned...
Article
Genetic analysis of 20 sterile mutants prone to form hyphae revealed 11 novel ste genes (sep6 to sep16) of Schizosaccharomyces pombe. None of the mutants was completely mycelial. Most mutants formed branching hyphae and showed normal septation. Aberrant septal structures and actin distribution were seen only at 36 degrees C. sep9-307, sep14-576 and...
Article
Genetic analysis of 20 sterile mutants prone to form hyphae revealed 11 novel ste genes (sep6 to sep16) of Schizosaccharomyces pombe. None of the mutants was completely mycelial. Most mutants formed branching hyphae and showed normal septation. Aberrant septal structures and actin distribution were seen only at 36°C. sep9-307, sep14-576 and sep15-5...
Article
The order Schizosaccharomycetales contains a dimorphic and two yeast species. Sch. japonicus can form both yeast cells and mycelium, depending on the substrate and the culturing conditions. Sch. pombe is a strictly unicellular organism, but it can be forced to form mycelial cell chains by inactivating members of the sep gene family. The mutations i...
Article
Maxillofacial fractures under 10 years of age are uncommon, with an occurrence of 7.3%. Isolated fractures of the mandible are even more rare, due to the fact that in young children the mandible is relatively small and resilient. Some authors suggest that the occurrence of this form of fracture is about 1-5%. Different methods have been described f...
Article
During the planning of implantation the CT method helps in determining the location of the implant and aids in choosing the right implant. It provides the following measurable information about the jaw-bones: height and width; exact location of bone deficiencies; the orovestibular shape of the alveolar process; the course of the mandibular canal; t...
Article
Correlation between bioclimatical and meteorological factors is well documented. The aim of this study was to investigate the correlation between dental periostitis and meteorological fronts, cyclon and anticyclon. We found a positive, statistically significant correlation between occurrence of warm meteorological front, cyclon, anticyclon and prev...
Article
The level of malondialdehyde, a stable end product of lipid peroxidation induced by reactive oxygen intermediates and the activity of two potent antioxidant enzymes, superoxide dismutase and glutathione peroxidase, was investigated in tissue homogenates of 22 surgical periapical granuloma specimens. Malondialdehyde levels were significantly higher...
Article
The authors describe an endosseal autotransplantation A tooth is extracted together with its parodontium and the surrounding bone. It is implanted into a different place where the bone had been prepared. Upper left unilateral free-end saddle cases were solved by removing the tooth No 24 into the place of No 27. A bridge was constructed after 6 week...
Article
Az egysejtes Schizosaccharomyces pombe ideális modellszervezet az eukarióta sejtciklus tanulmányozásához. A debreceni laboratórium több mint 30 sejtciklus-gént identifikált ebben a szervezetben. A jelenlegi projektben öt sep gén molekuláris biológiai vizsgálatát végezték el. Korábbi eredményeik alapján már tudták, hogy ezek a gének többféle funkció...
Article
A sejtosztódáshoz szukseges sok szaz gen periodikus mukodtetese, amelynek molekularis mechanizmusarol nagyon keveset tudunk. Azonban szamos rakos megbetegedes kialakulasaban megtalalhato ok a periodikusan mukodo genek szabalyozasanak hibaja, amely igy folyamatos aktivalast es kontrollalatlan osztodast eredmenyez. A kutatasi projekt soran egy, a per...

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