Zlatko Šatović

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• Professor
• Professor (Full) at University of Zagreb

The essential oil of Helichrysum italicum (Roth) G.Don, commonly known as immortelle, is produced in Mediterranean countries to meet the increasing demand of the cosmetic and pharmaceutical industries. This study focused on the analysis of secondary metabolites, specifically essential oils, extracted from plants grown from the seeds of natural immo...

This paper is aimed at investigating leaf morphological variability and possible hybridization between two species within the Rosaceae family: the one-seed hawthorn ( Crataegus monogyna Jacq.) and the almond-leaved pear ( Pyrus spinosa Forssk.). These two genera are taxonomically related and meet many prerequisites for successful hybridization betw...

The Balkan Peninsula represents an important center of plant diversity, exhibiting remarkable ecological heterogeneity that renders it an optimal region for studying the diversification patterns of complex taxa such as Teucrium montanum. In the Balkan Peninsula, T. montanum is a highly plastic and morphologically variable species with unresolved ta...

This study investigates genetic diversity among five morphotypes and five chemotypes of Ocimum basilicum L. (sweet basil) using amplified fragment length polymorphism (AFLP) markers. Conducted on 80 basil accessions from the Collection of Medicinal and Aromatic Plants of the National Plant Gene Bank of the Republic of Croatia, this research aims to...

Tradicijski kultivari graha (Phaseolus vulgaris L.) pokazuju različitu otpornost na niske temperature, što se očituje u njihovoj sposobnosti klijanja, rasta i prinosa. Ove su varijacije ključne za odabir odgovarajućih kultivara za specifične okolišne uvjete. Poželjne karakteristike uključuju brzo i ujednačeno klijanje i nicanje, čak i pri niskim te...

North Adriatic sweet chestnut (Castanea sativa Mill.) orchards are unique as in a relatively small area three different genetic groups can be found: cultivated trees, wild trees, and their natural crosses. Numerous investigations have focused on the morphology of cultivated and wild sweet chestnut trees, but their interactions remain underexplored....

Dalmatian sage (Salvia officinalis L., Lamiaceae) is a well-known aromatic and medicinal Mediterranean plant native to the coastal regions of the western Balkans and the central and southern Apennine Peninsula and is cultivated worldwide. A total of 1,350 specimens were collected and genotyped from 50 populations from the Balkan Peninsula and 12 fr...

Dalmatian pyrethrum (Tanacetum cinerariifolium (Trevir.) Sch. Bip) is a plant species that occurs naturally in the coastal and peri-coastal regions of the Balkan Peninsula (1). As a source of the natural repellent and insecticide pyrethrin, the natural populations have been collected and cultivated in the past. Twelve microsatellite markers (2) wer...

Southern Europe is home to two naturally occurring pear species: the European wild pear (Pyrus pyraster (L.) Burgsd.) and the almond-leaved pear (P. spinosa Forssk.). In addition to these two species, the cultivated pear (P. communis L.) is also grown throughout Europe. Since the cultivated pear is one of the most widespread fruits in Europe, gene...

The insecticidal compound pyrethrin is synthesized in Dalmatian pyrethrum (Tanacetum cinerariifolium (Trevis.) Sch.Bip.; Asteraceae), a plant species endemic to the eastern Mediterranean. Pyrethrin is a mixture of six compounds, pyrethrin I and II, cinerin I and II, and jasmolin I and II. For this study we sampled 15 natural Dalmatian pyrethrum pop...

Phenotypic differentiation is often the result of the ecological diversity of a species with a wide natural range. It is favoured by natural selection, as well as factors such as gene flow, genetic drift, or founder events. This is especially true for species of the riparian habitats and for species with light seeds that are easily dispersed by bot...

In this work, we analyzed the morphology and genetic structure of Teucrium montanum, T. capitatum and their hybrid T. × rohlenae from three syntopic populations. A morphometric study showed that the parents and their hybrids exhibited continuous morphological variation, with the hybrid positioned exactly between the parents. Genetic analysis reveal...

Teucrium montanum L. and T. capitatum L. are two plant species with overlapping distribution in the Balkan Peninsula, especially in Croatia, where several occurrences of their putative hybrid species T. × rohlenae have been recorded. Next-generation sequencing of both species and de novo assembly was carried out resulting in 120 contigs for T. mont...

Simple sequence repeats (SSRs) have become one of the most popular molecular markers and are used in numerous fields, including conservation genetics, population genetic studies, and genetic mapping. Advances in next-generation sequencing technology and the growing amount of genomic data are driving the development of bioinformatics tools for SSR m...

Citation: Klepo, T.; Benčić, Ð.; Liber, Z.; Belaj, A.; Strikić, F.; Kević, N.; Šatović, Z. Revealing the Diversity and Complex Relationships of Croatian Olive Germplasm. Int. J. Mol. Sci. 2024, 25, 3170. https://doi.org/ 10.3390/ijms25063170 Academic Editor: Abir U. Igamberdiev Abstract: Olive growing in Croatia has a long tradition and is of great...

Range contraction and habitat fragmentation can cause biodiversity loss by creating conditions that directly or indirectly affect the survival of plant populations. Fragmented habitats can alter pollinator guilds and impact their behavior, which may result in pollen/pollinator limitation and selection for increased selfing as a mechanism for reprod...

In mountain environments, populations of a species are subjected to different environmental conditions. Environmental variables impose different selection pressures that could result in a marked heterogeneity in genetic characteristics (e.g. ploidy level, genome size, population genetic diversity and structure), population features and reproductive...

Juniperus L. is a very diverse genus of dioecious or monoecious conifers distributed throughout the Mediterranean region. In addition to the complex taxonomic characteristics of the genus, sexual dimorphism leads to sex-specific differences in the morphology of juniper species, which further complicates the delimitation of species. Two of these spe...

Marginal populations are usually smaller and more isolated and grow in less favourable conditions than those at the distribution centre. The variability of these populations is of high importance, as it can support the adaptations needed for the conditions that they grow in. In this research, the morphological variability of eight Tatar maple (Acer...

In Spain, several local studies have highlighted the likely presence of unknown olive cultivars distinct from the approximately 260 ones previously described in the literature. Furthermore, recent advancements in identification techniques have significantly enhanced in terms of efficacy and precision. This scenario motivated a new nationwide prospe...

Many different natural habitats contribute to high biological diversity in Croatia. The diversity of the olive tree is very high, with numerous cultivated and wild olive accessions. Within the project "Biodiversity and Molecular Plant Breeding", research expeditions were conducted throughout the olive growing area of Croatia.

Croatian pedunculate oak (Quercus robur L.) populations represent southern range peripheral populations, often viewed as sources of valuable diversity and drought-resistant ecotypes. At the same time, they endure stronger selection pressures as a result of climate change. The leaves of 20 individuals per population (17) were sampled in a field tria...

Dalmatian (Salvia officinalis L.) and Greek sage (Salvia fruticosa Mill.) grow sympatrically on the island of Vis, Croatia. The island of Vis is the only part of Croatia where S. fruticosa occurs, in small, isolated populations, while S. officinalis is distributed throughout the eastern Adriatic coastal region. Both species synthesize essential oil...

Cilj je udžbenika „Očuvanje biljnih genetskih izvora“ objasniti teorijske i praktične aspekte očuvanja i održive upotrebe biljnih genetskih izvora. Proizvodnja hrane i drugih poljoprivrednih proizvoda temelji se na raspoloživosti biljnih genetskih izvora koji se koriste u svrhu oplemenjivanja novih, visokoprinosnih kultivara visoke kakvoće te otpor...

Dalmatian pyrethrum (Tanacetum cinerariifolium (Trevir.) Sch. Bip) occurs naturally in the coastal and peri-coastal regions of the Balkan Peninsula. Due to the production of pyrethrin (insecticide and repellent) in the flower heads, the species has been intensively cultivated in certain regions of the world (Japan, Australia, African countries) ove...

Southeast Europe (SEE) is a very important maize-growing region, comparable to the Corn belt region of the United States, with similar dent germplasm (dent by dent hybrids). Historically, this region has undergone several genetic material swaps, following the trends in the US, with one of the most significant swaps related to US aid programs after...

Sweet chestnut (Castanea sativa Mill.) is a noble, multi-purpose hardwood species. In addition to edible fruits and high-quality wood, chestnut forests have been used as a source of various resources since the antiquity. Intensive management of forests and stands, however, together with the emergence of chestnut blight, has led to their decline and...

Due to climate extremes and limited natural resources, especially water, we can expect increased demand in the future for species that can better tolerate climate extremes such as drought. One potentially valuable horticultural species is the endemic species of the Dinaride Mountains Micromeria croatica (Pers.) Schott (family Lamiaceae). It grows i...

Altitudinal gradients in mountain environments impose selection pressures that could result in marked heterogeneity in genetic characteristics, population features and reproductive success. Most Cactaceae occur in arid and semi-arid areas of the American continent, from sea level to 4500 m a.s.l., and some species have wide altitudinal ranges. The...

Plant biodiversity is the basis for sustainable agricultural production. Due to various geographical, climatic and soil conditions, Croatia has a great richness of medicinal and aromatic plants (MAP), whose natural populations show great diversity at the morphological, biochemical and genetic levels. However, they are threatened by genetic erosion...

The genus Teucrium is a source of beneficial bioactive phytochemicals and its use is well known in the traditional medicine of many countries. Studies on the wild populations of Teucrium species are scarce. Croatian flora numbers 12 accepted species of this genus, including a recently discovered hybrid between T. montanum and T. polium. This study...

Oplemenjivanje bilja dugotrajna je sofisticirana djelatnost koju obavlja osoblje sa specijaliziranim znanjima iz više znanstvenih područja i zahtijeva skupu infrastrukturu. Konačni su ishod oplemenjivanja bilja novi kultivari (sorte) poljoprivrednog bilja koji nastaju tijekom višegodišnjih ciklusa odabira. Novi, prinosniji kultivari, poboljšane kak...

The development of bioinformatic solutions is guided by biological knowledge of the subject. In some cases, we use unambiguous biological models, while in others we rely on assumptions. A commonly used assumption for genomes is that related species have similar genome sequences. This is even more obvious in the case of chloroplast genomes due to th...

A study of the phytochemical and molecular characteristics of ten Micromeria and six Clinopodium taxa (family Lamiaceae) distributed in the Balkan Peninsula was carried out. The phytochemicals detected in essential oils by gas chromatography, mass spectrometry, and molecular data amplified fragment length polymorphism were used to study the taxonom...

Common bean (Phaseolus vulgaris L.) is the most widespread legume in Croatia and its production is based on landraces of great morphological diversity. Landraces morphologically differ the most in the seed coat color and size. Because plant emergence and crop establishment represent the most sensitive stage in crop development, the aim of this stud...

The number of available DNA sequences in public genetic databases is constantly increasing. In plants, this is particularly evident in the amount of available complete chloroplast genomes, which are widely used in phylogenetic research. Chloroplast DNA genomes are circular and most have a four-part structure caused by two copies of a large inverted...

Dalmatian pyrethrum (Tanacetum cinerariifolium (Trevis.) Sch. Bip.) is an endemic species of the eastern Adriatic from the family Asteraceae. The plant produces pyrethrin, a secondary metabolite that has been shown to an effective natural insecticide with little or no adverse effects on humans. To assess genetic diversity, samples were collected fr...

Salvia officinalis L. occupies an important place in the Albanian market for aromatic medicinal plants due to the high content and composition of its essential oil and is the most economically important species for the country. The aim of this study was to investigate the genetic diversity and structure of eight populations of Dalmatian sage from A...

Pyrethrin is a leading plant biopesticide that is highly effective against numerous pests and has little or no negative impact on human health and the environment. It is a specialized metabolite from Dalmatian pyrethrum (Tanacetum cinerariifolium (Trevis.) Sch. Bip. ; Asteraceae), a plant species native to the eastern Adriatic coast. Pyrethrin cons...

Studying the population-genetic and phylogeographic structures of a representative species of a particular geographical region can not only provide us with information regarding its evolutionary history, but also improve our understanding of the evolutionary processes underlying the patterns of species diversity in that area. By analysing eight hig...

Željeli smo istražiti u kojoj mjeri današnje stanovništvo poznaje samoniklo jestivo bilje što raste na otoku, kojih se starinskih recepata Zlarinke sjećaju iz prošlih vremena te pripremaju li se još uvijek neka jela od samoniklog bilja. Težište je bilo upravo na mješavini od nekoliko (često i više od desetak) različitih biljaka koje se zajedno beru...

The development of next-generation sequencing technology and the increasing amount of sequencing data have brought the bioinformatic tools used in genome assembly into focus. The final step of the process is genome annotation, which works on assembled genome sequences to identify the location of genome features. In the case of organelle genomes, sp...

Gene flow between cultivated and wild gene pools is common in the contact zone between agricultural lands and natural habitats and can be used to study the development of adaptations and selection of novel varieties. This is likely the case in the northern Adriatic region, where centuries-old cultivated orchards of sweet chestnut ( Castanea sativa...

Lavandin (Lavandula × intermedia Emeric ex Loisel.) was brought to the Island of Hvar (Croatia) in the 1920s, coinciding with the beginning of large-scale cultivation of lavandin in France. Although the cultivation of lavandin and the production of essential oils are of great importance worldwide, the genetic diversity of lavandin has been little s...

Dalmatian pyrethrum (Tanacetum cinerariifolium (Trevir.) Sch. Bip.) is a plant species that occurs naturally in the eastern Mediterranean. It is of immense economic importance as it synthesizes and accumulates the phytochemical compound pyrethrin. Pyrethrin consists of several monoterpene esters (pyrethrin I and II, cinerin I and II and jasmolin I...

Dalmatian pyrethrum (Tanacetum cinerariifolium (Trevis.) Sch. Bip.) is an outcrossing plant species (2n = 18) endemic to the eastern Adriatic coast and source of the natural insecticide pyrethrin. Due to the high repeatability and large genome (1C-value = 9.58 pg) our previous attempts to develop microsatellite markers using the traditional method...

Dalmatian pyrethrum (Tanacetum cinerariifolium (Trevir.) Sch. Bip. ; Asteraceae), a source of the commercially dominant plant insecticide pyrethrin, is a species endemic to the eastern Adriatic. Genetic diversity of T. cinerariifolium was previously studied using amplified fragment length polymorphism (AFLP) markers. However, microsatellite markers...

Winter savory (Satureja montana L.) is an important medicinal, aromatic, and honey plant. In Croatia, it is widely distributed along the Adriatic coast, where it is frequently exposed to droughts. First, the winter savory natural population with the highest germination across different drought treatments after hydropriming was selected. Nine hundre...

The Pannonian Plain, as the most productive region of the South-East Europe, has a long tradition of agronomical production as well as agronomical research and plant breeding. Many research institutions from agri-food sector of this region have a significant impact on agriculture. Their well-developed and fruitful breeding programmes resulted in pr...

Tanacetum cinerariifolium is an endemic species of the eastern Adriatic coast that synthesizes the natural insecticide pyrethrin. We have characterized the complete chloroplast genome of the species and analyzed its phylogeny within the Asteraceae family. The complete chloroplast genome of T. cinerariifolium has a size of 150,136 bp, including a la...

Olive, the emblematic Mediterranean fruit crop, owns a great varietal diversity, which is maintained in ex situ field collections, such as the World Olive Germplasm Bank of Córdoba (WOGBC), Spain. Accurate identification of WOGBC, one of the world’s largest collections, is essential for efficient management and use of olive germplasm. The present s...

Almond-leaved willow (Salix triandra L., Salicaceae) is a dioecious shrub, rarely a small tree that grows under various environmental conditions. We examined the population structure of 12 populations of almond-leaved willow using nine leaf morphological traits and specific leaf area. Populations were selected from a range of habitats, from contine...

Leaves play a central role in plant fitness, allowing efficient light capture, gas exchange and thermoregulation, ensuring optimal growing conditions for the plant. Phenotypic variability in leaf shape and size has been linked to environmental heterogeneity and habitat characteristics. Therefore, the study of foliar morphology in plant populations...

Pyrethrin is a specialized metabolite of Dalmatian pyrethrum (Tanacetum cinerariifolium (Trevir.) Sch. Bip.), Asteraceae, known worldwide as an effective bioinsecticide. It consists of six active compounds: Pyrethrin I and II, cinerin I and II, and jasmolin I and II. Pyrethrin accumulates mainly in the flower heads and its content depends on numero...

Common or English yew (Taxus baccata L., Taxaceae) is a conifer species, native to Europe, northern Africa, Asia Minor and Caucasus. It is a dioecious, wind-pollinated and animal-dispersed tree, known for its high-quality wood and medicinal properties, albeit poisonous. The species is rare and has been legally protected at the European and national...

Sweet chestnut (Castanea sativa Mill., Fagaceae) is one of the oldest cultivated tree species in the Mediterranean, providing multiple benefits, and, since it has edible seeds, it represents an interesting model species for the research of morphological and chemical variability. In this study, morphometric methods and chemical analyses were used to...

Immortelle (Helichrysum italicum (Roth) G. Don; Asteraceae) is a perennial plant species native to the Mediterranean region, known for many properties with wide application mainly in perfume and cosmetic industry. A total of 18 wild H. italicum populations systematically sampled along the eastern Adriatic environmental gradient were studied using A...

Almond-leaved pear (Pyrus spinosa Forssk., Rosaceae) is a scientifically poorly researched and often overlooked Mediterranean species. It is an insect-pollinated and animal-dispersed spiny, deciduous shrub or a small tree, with high-quality wood and edible fruits. The aim of the study was to assess the phenotypic diversity of almond-leaved pear in...

Grass pea (Lathyrus sativus L.) is an annual legume species, phylogenetically close to pea (Pisum sativum L.), that may be infected by Fusarium oxysporum f. sp. pisi (Fop), the causal agent of fusarium wilt in peas with vast worldwide yield losses. A range of responses varying from high resistance to susceptibility to this pathogen has been reporte...

Pyrethrin is a potent biopesticide, a natural mixture of six compounds (pyrethrin I and II, cinerin I and II, and jasmolin I and II), biosynthesized in plants of Dalmatian pyrethrum (Tanacetum cinerariifolium/Trevir./Sch. Bip.), a species endemic to the eastern Adriatic coast, but grown worldwide. Not many known natural insecticides encompass such...

Seeds collected from 10 Dalmatian pyrethrum (Tanacetum cinerariifolium /Trevir./ Sch. Bip. Asteraceae) populations naturally occurring along the Adriatic coast and islands, were used to establish a common garden with the aim of estimating the variation of pyrethrin compounds between and within populations. A recently optimized matrix solid phase di...

Hop (Humulus lupulus L.) is used in the brewing industry as a source of compounds responsible for the bitterness, aroma, and preservative properties of beer. In this study, we used microsatellite markers to investigate genetic diversity and genetic differentiation of wild hop populations sampled in the northwestern part of Croatia. Analysis of 12 m...

With the development of next-generation sequencing technology and bioinformatics tools, the process of assembling DNA sequences has become cheaper and easier, especially in the case of much shorter organelle genomes. The number of available DNA sequences of complete chloroplast genomes in public genetic databases is constantly increasing and the da...

Neven ( Calendula officinalis L.) je poznat po svojim ljekovitim svojstvima kao i primjeni u medicinskoj, kemijskoj, kozmetičkoj i prehrambenoj industriji. Prilagođen je umjerenoj klimi te mu je optimalna temperatura klijanja 20 °C. Klijanje sjemena i rani rast sadnica su najosjetljiviji stadiji na abiotske stresove, kao što su nepovoljna temperatu...

Olea europaea ‘Galega vulgar’ variety is a blend of West and Central Mediterranean germplasm with cultivated-wild admixture characteristics. ‘Galega vulgar’ is known for its high rusticity and superior-quality olive oil, being the main Portuguese variety with high impact for bioeconomy. Nevertheless, it has been replaced by higher-yielding and more...

Sweet pepper (Capsicum annuum L.) is one of the most important vegetable crops in the world because of the nutritional value of its fruits and its economic importance. Calcium (Ca) improves the quality of sweet pepper fruits, and the application of calcite nanoparticles in agricultural practice has a positive effect on the morphological, physiologi...

This study examines genetic diversity and structure of a Croatian garlic germplasm collection using 13 simple sequence repeat (SSR) markers. A total of 71 alleles were observed across 64 accessions representing 3 Croatian regions (Istria, Dalmatia and continental Croatia) and 16 foreign landraces, with an average of 5.46 alleles per locus. Among th...

Selection for wheat (Triticum aestivum L.) grain quality is often costly and time-consuming since it requires extensive phenotyping in the last phases of development of new lines and cultivars. The development of high-throughput genotyping in the last decade enabled reliable and rapid predictions of breeding values based only on marker information....

Landraces represent valuable genetic resources for breeding programmes to produce high-yielding varieties adapted to stressful environmental conditions. Although the common bean (Phaseolus vulgaris L.) is an economically important food legume for direct human consumption worldwide, common bean production in Croatia is based almost exclusively on la...

Microsatellites (SSRs) are highly informative repetitive sequences of 2-6 base pairs, which are the most used molecular markers in assessing the genetic diversity of plant species. Dalmatian pyrethrum (Tanacetum cinerariifolium /Trevir./ Sch. Bip) is an outcrossing diploid (2n = 18), endemic to the eastern Adriatic coast and source of the natural i...

Micronutrient malnutrition is one of the main public health problems in many parts of the world. This problem raises the attention of all valuable sources of micronutrients for the human diet, such as common bean (Phaseolus vulgaris L.). In this research, a panel of 174 accessions representing Croatian common bean landraces was phenotyped for seed...

Dalmatian pyrethrum (Tanacetum cinerariifolium /Trevir./ Sch. Bip.), a species endemic to the eastern Adriatic coastline, is the source of natural insecticide pyrethrin. Pyrethrin is a mixture of six compounds (pyrethrin I and II, cinerin I and II, jasmolin I and II) that exhibits high insecticidal activity, with no detrimental effects to the envir...

Basil is one of the most widespread aromatic and medicinal plants, which is often grown in drought- and salinity-prone regions. Often co-occurrence of drought and salinity stresses in agroecosystems and similarities of symptoms which they cause on plants complicates the differentiation among them. Development of automated phenotyping techniques wit...

Abstract: More than one third of European grain maize is produced in South Eastearn Europe (SEE) and utilization of historical maize material developed in SEE for its favorable alleles and diversity has long been speculated. However, molecular information on diversity of the SEE maize genetic material is scarce. The objectives of this study were i)...

Pyrethrin is a mixture of six compounds (pyrethrin I and II, cinerin I and II, jasmolin I and II) with high insecticidal potential. Due to the low toxicity to humans and high efficiency in dealing with various insect species, they are often used in organic pest management. yrethrins are extracted from the flower heads of Dalmatian pyrethrum (Tanac...

By performing a high-resolution spatial-genetic analysis of a partially clonal Salvia brachyodon population, we elucidated its clonal architecture and seedling recruitment strategy. The sampling of the entire population was based on a 1 × 1 m grid and each sampled individual was genotyped. Population-genetic statistics were combined with geospatial...

Essential oils from seven populations of Dalmatian sage from the North of Albania were extracted and analyzed according European Pharmacopeia 7.0. The yield differed from 1.95 to 2.75% v/w. The GC-FID-MS analysis of the sage oil revealed 42 constituents. Camphor (20.50-29.74%), followed by α-thujone (19.87-24.29%), 1,8-cineole (6.88-12.64%) and β-t...

The high-biodiversity phenomenon of the Balkan Peninsula is a result of the past and present topographic, climatic and geological conditions, as well as human impact. The Dinaric Alps, as a part of the Balkan Peninsula, harbour a high number of endemic species, some of them comprising a small number of populations, which are often endangered. We in...

Campanula fenestrellata subsp. fenestrellata and C. fenestrellata subsp. istriaca belong to the morphologically and phylogenetically well established amphi-Adriatic group Campanula ser. Garganicae. The two morphologically differentiated endemic taxa are distributed on the north-eastern Adriatic islands and coast. In order to study the relationships...

Salvia officinalis is a perennial species, native and endemic to the Western Balkans and the Apennine Peninsula. Due to its medicinal and aromatic properties, it is used in pharmaceutical, cosmetic, and food industries. The main objectives of the study were to infer the genetic structure of S. officinalis populations in the northern and central par...