Zizy Elbialy

Faculty of Aquatic and Fisheries Sciences, Kafrelsheikh University, Egypt · Fish processing and Biotechnology

These authors contributed equally to this work. Simple Summary: Fatty liver injury is common in farmed fish, but its molecular mechanisms are not well understood. An eight-week feeding trial was conducted to investigate the effects of dietary chitosan on high-fat diet (HFD)-induced liver damage in Nile tilapia. Six diets with varying fat and chitos...

As Fish is a crucial and diverse protein source in many diets, its high susceptibility to spoilage necessitates close attention to quality and safety. This study assessed the quality and safety of both imported and locally sourced fish found in Egyptian markets through physico-chemical, microbiological, and sensory analyses. While acceptable pH lev...

This study aimed to assess the impact of seasonal changes on heavy metal concentrations in water and their effects on Nile Tilapia (Oreochromis niloticus) liver and gill histomorphology in Burullus Lake, Egypt in 2021. Six designated points were selected for water and fish (length ±5cm) sampling in both summer and winter. The results indicated that...

This study aimed to determine the effect of dietary inclusion of chitosan (CTZ) on semen variables, sperm apoptosis and DNA fragmentation, and seminal plasma antioxidant status of Zaraibi goat bucks fed a high-fat diet. A total of 15 mature bucks (39.42±0.48 kg LBW and 18-30 mo old) were divided into 3 groups (n= 5); the control (CG) and two of the...

This study aimed to investigate the gene expression levels associated with nephrotoxic action of amikacin, as well as the post-treatment effect of diuretics on its nephrotoxic effects. Sixty male rats were divided equally into six groups, including the control group receiving saline intra-peritoneally (ip), and the five treated groups including the...

This study was conducted to explore the effects of dietary inclusion of Chlorella vulgaris (CV) or/and vitamin C (VC) on growth, hemato-biochemical parameters, oxidative and antioxidant status, reproductive hormones, and semen quality variables , and scrotal-testicular dimensions of Zaraibi goat bucks. Twenty sexually mature bucks (41.49 ± 0.91 kg...

Olive leaves are an immense source of antioxidant and antimicrobial bioactive constituents. This study investigated the effects of dietary incorporation of olive leaf extract (OLE) on the growth performance, hematobiochemical parameters, immune response, antioxidant defense, histopathological changes, and some growth- and immune-related genes in th...

The antioxidant and detoxification systems involve intricate pathways in which nuclear factor erythroid 2-related factor 2 (Nrf2) and Kelch-like ECH-associated protein 1 (Keap1) play pivotal roles. In the basal state, reactive oxygen species are generated and neutralized in a balanced manner. However, stressors can disrupt this equilibrium, resulti...

Spirulina is generally used as a nutraceutical feed supplement due to its nutrient profile, lack of toxicity, and therapeutic effects. This study aimed to explore the effect of dietary inclusion of Spirulina platensis (SPL) algae on growth, hemato-biochemical parameters, antioxidant status, and semen quality of cross-bred lambs (½ Finnish × ½ Ossim...

Recently, chitosan gained a great attention due to its unique biological activities as a natural biodegradable polymer derived from chitin with non-antigenic, non-toxic. It has several positive impacts on animal health including potent antioxidant, antimicrobial activities, and anti-immunogenicity. Therefore, it is a natural, bioactive, mucoadhesiv...

This study focuses on the relationship between myostatin (MyoS), myogenin (MyoG), and the growth hormone/insulin-like growth factor-1 (GH/IGF-1) axis for muscle growth and histopathological changes in muscle after an Aeromonas hydrophila infection. A total number of 90 Nile tilapia (55.85 g) were randomly allocated into two equal groups of three re...

This study was conducted to explore the effects of dietary inclusion of Chlorella vulgaris (CV) or/and vitamin C (VC) on growth, hemato-biochemical parameters, oxidative and antioxidant status, reproductive hormones, and semen quality variables, and scrotal-testicular dimensions of Zaraibi goat bucks. Twenty sexually mature bucks (41.49±0.91 kg BW)...

In medicine, silver nanoparticles (AgNPs) are employed often. They do, however, have negative impacts, particularly on the reproductive organs. This research aimed to assess AgNP impact on the testis and the possible intracellular mechanisms to induce testicular deteriorations in rats at various concentrations and different time intervals. Sprague...

The ameliorative effects of Spirulina and Saccharomyces cerevisiae (S. cerevisiae) against fipronil toxicity in Nile tilapia fish were investigated. Fipronil is a kind of pesticide that is widely used in agriculture, thus this trial was conducted to evaluate the effect of fipronil on growth related parameters (final body weight, feed intake, weight...

The current study investigated how different fasting and refeeding regimes would impact Nile tilapia growth performance, histopathological examination, and gene expression of myostatin, myogenin, GH , IGF-1 , and NPYa . Nile tilapia fish ( n = 120) were randomly allocated into four groups, including the control group fed on a basal diet for 6 weeks...

Despite the extraordinary use of silver nanoparticles (AgNPs) in medicinal purposes and the food industry, there is rising worry about potential hazards to human health and the environment. The existing study aims to assess the hepatotoxic effects of different dosages of AgNPs by evaluating hematobiochemical parameters, oxidative stress, liver morp...

Aiming to boost the catfish fillet quality, the consequences of their treatment with Lepidium sativum seeds mucilage (LSSM) and with LSSM-mediated selenium nanoparticles (LSSM-Se NPs) were investigated. The LSSM assessment for phenolic acids contents emphasized higher concentrations. Green-synthesized Se NPs were conjugated with LSSM to form LSSM-S...

Ochratoxin A (OTA) is one of the most dangerous and that pollute agricultural products, inducing a variety of toxic effects in humans and animals. The current study explored the protective effect of different concentrations of Aspergillus awamori ( A . awamori ) against OTA (0.3 mg/kg diet) induced renal and cardiac damage by exploring its mechanis...

Recently, the high cost of aquafeed affected fish farming feasibility in some countries, including Egypt. The imported soybean meal and corn ingredients consume a large amount of the hard currency, thereby increasing feed prices. Thus, the current study investigated the different sources of soybean and corn on the performances of Nile tilapia. Fish...

Wound healing is a public health concern. Licorice gained a great attention for its antioxidant and anti-inflammatory properties which expand its valuable effects as a herbal medicine. In this study, we pointed out to the wound healing potential and the mechanism by which licorice alcoholic extract can modulate cutaneous wound healing through immun...

Ammonia is a critical hazardous nitrogen metabolic product in aquaculture. Despite trials for its control, ammonia intoxication remains one of the most critical issues to overcome. In this study, we explored the modulatory effect and potential mechanism by which Yucca schidigera extract (YSE) can ameliorate ammonia intoxication-induced adverse effe...

Hepatocellular carcinoma (HCC) is the most common cancer in humans. Despite advances in its treatment, liver cancer remains one of the most difficult cancers to treat. This study aimed to investigate the ameliorative action and potential mechanism of Aspergillus awamori (ASP) administration against the initiation process of liver carcinogenesis ind...

The present study evaluated the enhancement of the growth rate, intestinal health, immune-related genes, and resistance against suboptimal water temperature in common carp (2.01 ± 0.02 g) by dietary paraprobiotics. Fish were fed dietary heat killed Lactobacillus plantarum (LP20) at 0 (control), 50, and 100 mg/kg for 2 months. After the feeding tria...

Blue-green microalga Spirulina platensis (SP) gained more attention for its antioxidant and/or anti-inflammatory properties magnifying its beneficial effects as a feed additive and for cosmetic and biomedical applications. This study was performed to examine the impact of SP on the cutaneous wound and burn healing and to develop an understanding of...

The main goal of this study was to estimate genetic differences of samples of Farmed and wild Gilthead Sea bream at the Suez Canal region. A total of forty-eight samples have been collected with average weight ranged from 250-300 gm. Sixteen Morphometric traits were measured individually (Weight, Total length, Forked length, Standard length, Caudal...

This study was carried out to investigate the effect of Ulva fasciata methanol extract (UFME), as a dietary supplement on growth performance and growth‐related gene expression in liver, intestinal morphometry, haemato‐biochemical profile, immune response and oxidative status of Nile tilapia (Oreochromis niloticus). Four triplicate groups (Control,...

The main goal of this study was to estimate genetic differences of samples of European Sea bass from three different locations (the Red Sea, Mediterranean and Suez Canal). A total of sixty-four samples have been collected during the period from October to January… with an average weight ranging from 250-300 gm. Morphometric traits such as fish weig...

The present study investigated influence of Yucca schidigera extract (Ys E) on the growth performance, hepato‐renal function, antioxidative status and histopathological alterations in Nile tilapia (Oreochromis niloticus) exposed to hypoxia stress. 120 Nile tilapia fish (42.39 ± 1.25 g) were distributed into four equal groups, where group (a) kept a...

The present investigation aimed to evaluate the influence of selenium nanoparticles (Se-NPs) or/and spirulina (SP) on the growth, immunity, and oxidation resistance of Nile tilapia. Four groups of fish fed diets with Se-NPs or/and SP at 0 g (control), 1 g SP/kg diet (SP), 1 mg Se-NPs/kg diet (Se-NPs), and 1 g SP + 1 mg Se-NPs/kg diet (SP/Se-NPs) fo...

The present investigation evaluated the negative impacts of an aflatoxin-contaminated diet on the growth per-formance, serum and tissue biochemistry, gene expression of some immune-related genes in the liver, and pathological lesions in some organs of Nile tilapia. Moreover, the ameliorative effects of Moringa oleifera against fish aflatoxicosis we...

The main goal of this study to estimate genetic differences of samples of European Sea bass from three different locations (Red Sea, Mediterranean and Suez Canal). A total of 64 samples have been collected with average weight ranged from 250-300 gm. Morphometric traits such as fish weight, body length, tail length and others were measured. From the...

Background: Collagen is the most abundant structural protein in the mammalian connective tissue and represents approximately 30% of animal protein. The current study evaluated the potential capacity of collagen extract derived from Nile tilapia skin in improving the cutaneous wound healing in rats and investigated the underlying possible mechanism...

Chlorpyrifos (CPF) is an insecticide that is commonly applied in the agriculture sector. However, little is known about the protective role of Spirulina platensis (SP) and/or β-glucan (BG) on African catfish exposed to chronic CPF toxicity. The fish (95 ± 5 g, initial weight) were assigned to 5 fiberglass tanks (500 L, 50 fish/tank) where the 1st a...

The current study was conducted to evaluate the synergetic effects of heat-killed Lactobacillus plantarum (HK L-137) and β-glucan (BG) on digestive enzyme activity and intestinal morphology of genetically improved farmed tilapia (GIFT) with focus on insulin-like growth factor I (IGF-I), fatty acid synthase (FAS), and glucose-6-phosphate dehydrogena...

Triticale is a promising plant ingredient as a source of protein and carbohydrates which is recommended for aquafeed. Therefore, five isonitrogenous and isolipidic diets were formulated to include triticale at 0, 5, 10, 15, and 20% and fed for Nile tilapia (14.4 ± 0.02 g) for 60 days. The obtained results revealed that no significant linear or quad...

A trial was operated to assess the potential of using Lactobacillus plantarum L-137 (L-137) and/or β-glucan (BG) in improving the resistance of Nile tilapia against Aeromonas hydrophila. Control diet and 3 diets supplemented with L-137, BG or L-137 + BG were prepared. Final body weight, specific growth rate, superoxide dismutase, and catalase showe...

The current study aimed to evaluate whether clenbuterol, a β2-adrenergic agonist, supplementation in Nile tilapia (Oreochromis niloticus) diets can influence growth and blood parameters. Besides, assessment of adipogenic genes as fatty acid synthase (FAS) and lipoprotein lipase (LPL) which is a key enzyme in the regulation of the flux of fatty acid...

This study aimed to evaluate the growth, intestinal morphometry, immune-related genes, and heat shock protein in Nile tilapia (Oreochromis niloticus) reared at a rate of 10 (low density—LD, 200/m³), 20 (medium density—MD, 400/m³), and 30 (high density—HD, 600/m³) fish per aquarium (50 L). The fish were fed two diets [control and β-glucan (BG)] (2 ×...

The present study investigated the effects of sodium butyrate (SB) on the growth performance, histomorphology, immune response, and stress related markers of Nile tilapia subjected to heat stress. SB was incorporated at 0, 0.5, 1, 1.5, and 2 g per kg diet and fed to fish for 8 weeks. The obtained results revealed significantly improved growth perfo...

The present trial investigated the effects of dietary fenugreek on the histopathology, oxidative status, and immune-related gene expression in Nile tilapia (Oreochromis niloticus) (15 ± 0.5 g) infected with Aeromonas hydrophila. Fish were fed two diets with or without fenugreek supplementation at 3%. The trial was lasted for 8 weeks then fish were...

The present study was intended for investigating the effect of lead acetate toxicity (5 or 10 mg lead acetate/liter water), Dunaliella algae (11 g/kg diet)-supplemented diet and their interaction in Nile tilapia (60 days). 180 fish were divided into six groups according to lead concentration and Dunaliella supplementation. Results revealed that lea...

The current experiment was randomly designed in a 2×2 factorial design. Two dietary protein sources were utilized; fish meal and soybean meal; with betaine incorporation to both of them. This study aimed to determine the impact of incorporation of beta-ine into soybean meal-based (SBM) diets through its effects on growth performance, intestinal hea...

Background: Bromuconazole is a widely used triazole pesticide which is toxic not only to target fungi but also to animals and human. Aim: The present study aimed to evaluate the harmful effects of prolonged administration of bromuconazole fungicide-induced testicular toxicity in male rats. Methods: Rats were randomly allocated into 5 groups (10 rat...

The present study aimed to investigate the toxic effect of aflatoxin B1 (AFB1) and the effectiveness of a biological mycotoxin binder (Nutritox®) in detoxifying aflatoxicosis. Ninety one-day-old chicks were chosen and divided into 3 groups. The first group received standard basal diet only, the second group received a basal diet with AFB1 (0.25 mg/...

This study was conducted to investigate the effect of dietary supplementation of Aspergillus awamori on aflatoxin B>1> (AFB>1>)-induced liver damage in rabbits. Administration of AFB>1> (0.3 mg/kg diet) led to a significant reduction in body weight, body weight gain, total feed intake, total serum proteins, albumin, high density lipoprotein-cholest...

Despite widespread use of bromuconazole as a pesticide for food crops and fruits, limited studies have been done to evaluate its toxic effects. Here, we evaluated the hepatotoxic effect of bromuconazole using classical toxicological (biochemical analysis and histopathological examination) and gene-based molecular methods. Male rats were treated eit...

Myostatin (MSTN) gene is a negative regulator of skeletal muscle. In this study, we try to evaluate MSTN as a candidate gene for a marker assisted selection in Nile tilapia growth trait. Molecular characterization of MSTN gene was done using seven designed primers. We demonstrated that, O. niloticus MSTN sequence and its promoter is as for all know...

Fry production in Nile tilapia, Oreochromis niloticus is typically achieved by mass spawning of males and females stocked in large hapas suspended in ponds. In such spawning aggregations, territorial behavior and reproductive competition among males may lead to a large variance in reproductive success among individual males. We analyzed parentage a...

Territorial behavior and reproductive competition among Oreochromis niloticus males for fertilizing a batch of eggs from a female may lead to a large skew in reproductive success among individuals. However, there are no studies that have analyzed the reproductive success of individual males in spawning aggregations of Nile tilapia. We analyzed pare...