Ziyu Han

Tianjin University | tju · State Key Laboratory of Precision Measurement Technology and Instruments

Compared to animal cells, phenotypic characterization of single plant cells on microfluidic platforms is still rare. In this work, we performed population statistics on the morphological, biochemical, physical and electrical...

Hydrodynamic force loading platforms based on acoustofluidics have been developed to study the mechanical deformation of cancer cells and to control cell behavior. However, so far there have been no experimental measurements on living plant cells using such an acoustic approach. Unique structures, including cell walls, allow plant cells to exhibit...

The generation and dispensing of microdroplets is a vital process in various fields such as biomedicine, medical diagnosis and chemistry. However, most methods still require the structures of nozzles or microchannels to assist droplet generation, which leads to limitations on system flexibility and restrictions on the size range of the generated dr...

Microfluidic phenotyping methods have been of vital importance for cellular characterization, especially for evaluating single cells. In order to study the deformability of a single cell, we devised and tested a tunable microfluidic chip-based method. A pneumatic polymer polydimethylsiloxane (PDMS) membrane was designed and fabricated abutting a si...

Viral particles bind to receptors through multivalent protein interactions. Such high avidity interactions on sensor surfaces are less studied. In this work, three polyelectrolytes that can form biosensing surfaces with different interfacial characteristics in probe density and spatial arrangement were designed. Quartz crystal microbalance, interfe...

Nanochannel-based resistive pulse sensing (nano-RPS) system is widely used for the high-sensitive measurement and characterization of nanoscale biological particles and biomolecules due to its high surface to volume ratio. However, the geometric dimensions and surface properties of nanochannel are usually fixed, which limit the detections within pa...

A better understanding of the phenotypic heterogeneity of protoplasts requires a comprehensive analysis of the morphological and metabolic characteristics of many individual cells. In this study, we developed a microfluidic flow cytometry with fluorescence sensor for functional characterization and phenotyping of protoplasts to allow an unbiased as...

According to the urgent need for rapid detection and identification of foodborne bacteria to prevent public health event, a microfluidic electrical impedance flow cytometry assisted with convolutional neural network (ConvNet) based deep learning algorithm was proposed in this study to analyze the impedance signals of bacteria. With the assistance o...

This paper reports an IC-compatible method for fabricating a PDMS-based resistive pulse sensing (RPS) device with embedded nanochannel (nanochannel-RPS) for label-free analysis of biomolecules and bionanoparticles, such as plasmid DNAs and exosomes. Here, a multilayer lithography process was proposed to fabricate the PDMS mold for the microfluidic...

Despite that single-cell-type-level analyses have been extensively conducted on animal models to gain new insight into complex biological processes, the unique biological and physiological properties of plant cells have not been widely studied at single-cell resolution. In this work, an electrical impedance flow cytometry was fabricated based on mi...

We have developed a new three-dimensional (3D) surface for use in biosensors that is based on modified novel thorns-like polyelectrolytes (3D-PETx), which comprises of poly-l-lysine (PLL) appended with multitude oligo (ethylene glycol) (OEG) and biotin moieties. It tethered to the sensor surface by PLL, while the OEG-biotin chains are forced to str...

Primary cell wall (PCW) is a rigid yet flexible cell wall surrounding plant cells and it plays key roles in plant growth, cell differentiation, intercellular communication, water movement and defence. As a technique widely used to study the characteristics of mammalian cells, electrical impedance spectroscopy (EIS) is rarely used in plant science....

In article 1900671, Qiannan Xue, Xuexin Duan, and co‐workers demonstrate a nanoscale impedimetric immunosensor based on biotin doped conductive polymer nanowire arrays which are fabricated through simple solution printing approach. This sensor can be a general platform for various bio‐analytical applications with ultrahigh sensitivity.

The demand for fast and ultratrace biomarkers detection is increasing in bioanalytical chemistry. In this work, highly ordered nanowires array and sensor integration are achieved with nanoscale printing approach. Negatively charged poly(3,4‐ethylenedioxythiophene)–poly(styrenesulfonate) doped with positively charged PEGylated biotin‐derivatized pol...

One-dimensional organic nanostructures are essential building blocks for high performance gas sensors. Constucting as e-nose type sensor array is the current golden standard in develping portable system for gas mixtures detection. However, facile fabrication of nanoscale sensor array is still challenging due to the high cost of the conventional nan...

We report the nonlinear acoustic streaming effect and the fast manipulation of microparticles by microelectromechanical Lamb-wave resonators in a microliter droplet. The device, consisting of four Lamb-wave resonators on a silicon die, generates cylindrical traveling waves in a liquid and efficiently drives nine horizontal vortices within a 1−μl dr...

Supplementary figures and tables.

This work reported a wireless controlled micro-actuator system for rapid heating and mixing of multiple droplets using integrated arrays of micro-fabricated 2.5 GHz solid-mounted thin-film piezoelectric resonators (SMRs) and a millimeter-scale omnidirectional antenna. An equivalent circuit is proposed to analyze the mechanism of the heating, mixing...

This paper reports a surface functionalization strategy for protein detections based on biotin-derivatized poly(L-lysine)-grafted oligo-ethylene glycol (PLL-g-OEGx-Biotin) copolymers. Such strategy can be used to attach the biomolecule receptors in a reproducible way simply by incubation of the transducer element in a solution containing such copol...

A new surface functionalization scheme for nano-Bio field effect transistors (FETs) using biocompatible polyelectrolyte thin films (PET) is developed. PET assemblies on Si nanowires (Si-NWs) are driven by electrostatic interactions between the positively charged polymer backbone and negatively charged Si/SiO2 surface. Such assemblies can be directl...