Zisheng Luo

Zhejiang University | ZJU · Department of Food and Nutrition Science

Direct electrochemical analysis of bisphenol A (BPA) in alcoholic drinks without proper sample preparation is a major challenge. The current work reports a nanoporous electrode design with ultrathin polydimethylsiloxane (PDMS) coating grafted on the top of an indium tin oxide (ITO) electrode with silica nanochannels membrane and cetyltrimethylammon...

Epicuticular wax is the natural protective coating of fruit which is vulnerable to insect, microbe, pollutant, or scrape incidence. Herein, the epicuticular wax of table grapes was removed to mimic the natural wax loss/damage, and the quality attributes of fruit were investigated. Results demonstrated wax removal triggered fruit weight loss, soften...

Prochloraz (PTIC) is a hazardous fungicide used worldwide on agricultural produce despite concerns about potential impacts on human health and environmental pollution. The residue of PTIC and its metabolite 2,4,6-trichlorophenol (2,4,6-TCP) in fresh produce has largely not been clarified. Herein, we address this research gap by examining residues o...

With rapid growing of environmental contact infection, more and more attentions are focused on the precise and absolute quantification of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) virus on cold chain foods via point-of-care test (POCT). In this work, we propose a hydrogel-mediated reverse transcription loop-mediated isothermal am...

Background Nowadays, consumers increasingly prefer food with original, natural, and delicious flavors, which is closely linked to the content of volatile organic compounds (VOCs) in food. In recent years, the function of plant VOCs as natural food additives (flavoring and aromatizing agents, antibacterial agents) and their health benefits have attr...

Lipidomics enables high-throughput screening of lipidic compounds in biological samples that is potential to reveal the molecular mechanism from the perspective of lipidic metabolism and to discover potential biomarkers. Current study applied lipidomic analysis to unveil the lipid-associated bioprocesses in postharvest strawberry-gray mold interact...

Chlorine dioxide (ClO2) unexpectedly contributed to the yellowing alleviation of ready-to-eat broccoli. Herein, we employed phenotypic and transcriptomic analyses for the first time to investigate the molecular mechanism of yellowing alleviation in the presence of ClO2. In our study, ClO2 and chloroxyanions were mainly distributed in floret bud and...

DNA methylation is increasingly known to be essential for fruit ripening and senescence. Currently, 5-azacytidine (AZ) was selected as an effective demethylator and it successfully shaped the genomic hypomethylation in harvested strawberries. This was associated with the reprogramming of global gene expressions, which influenced downstream food tra...

The impact of red and blue light-emitting diodes (LEDs) irradiation on the sugar metabolism and γ-aminobutyric acid in postharvest table grapes stored at 4 °C for 21 days after harvest were explored. Grape clusters were exposed to red and blue light irradiation at the same intensity (500 lx). The findings revealed that red and blue light treatments...

Elevated CO2 storage causes overconsumption of reducing sugar and lower energy level in postharvest strawberry fruit, reducing the acceptance of this fruit. In order to develop an approach to improve the impaired quality, we applied additional fumigation of SO2 in combination with elevated CO2 storage to postharvest strawberry fruit in the present...

This work presented a novel strategy to integrate colorant and stabilizing effects of BSA-crocin I (BC) nanoparticles with gel-like structure of high internal phase Pickering emulsion (HIPPE, φ = 0.75). The BSA was cross-lined with crocin I to obtain BC nanoparticles for stabilizing HIPPE. When pH = 7.4, the particle size of BC nanoparticles was 32...

Lipoxygenase (LOX) pathway is the key and the most important pathway for the metabolism of fruit volatile. However, the transcription mechanism of the LOX pathway is still obscure in non-climacteric fruit. In order to identify the transcription factor involved in LOX-derived volatile metabolism, RNA sequencing and metabolome were used to profile st...

The presence of watercored fruit with translucent mesocarp has attracted immense attention due to its unique morphology and taste, however, the metabolic reconstruction between watercored and non-watercored tissues remain elusive. Herein, the combined proteomic and metabolomic approach was carried out to characterize the protein abundance and metab...

Camellia oleifera shells are abundant in polyphenolic compounds. Green extraction methods of polyphenolic compounds are essential to ensure product quality, efficiency, process cost, environment, and safety. This study investigated the effect of Tween 80 and Rhamnolipid surfactants on the production and utilization of stabilized carbon dioxide nano...

Foodborne pathogenic microorganisms have become major threats that endanger human life and health. The current technology cannot perform rapid screening of foodborne pathogenic bacteria, and fail to timely control food safety risks. Here, we develop a novel microfluidic sensor for real-time and label-free bacteria classification at the single-cell...

In the present study, fresh edible peanuts were packaged with nitrogen and oxygen (N2: O2 = 9:1) (modified atmosphere packaging, MAP) or air (control) and stored at 4 °C for 120 d. The physio-chemical properties, enzyme activities, and antioxidant capacity of fresh peanut kernels were analyzed at different time points during storage to investigate...

Auxin is involved in regulating the ripening process of tomato fruit, and sucrose metabolism is a vital part of the quality formation during tomato ripening. In comparison, the comprehensive effect of auxin on regulating sucrose metabolism during postharvest tomato fruit ripening remains to be clarified. In this research, tomato fruit at the mature...

Due to the global use of cold chain, the development of postharvest technology to reduce chilling injury (CI) in postharvest fruits and vegetables during storage and transport is needed urgently. Considerable evidence shows that maintaining intracellular adenosine triphosphate (iATP) in harvested fruits and vegetables is beneficial to inhibiting CI...

Sulfur dioxide (SO2) and its derivatives are known to be hazardous but their common application in food, especially the grape industry, is conditionally allowed. Potential hazards to consumers and the environment could occur upon the control-lost SO2 during grape logistics and storage. Researchers have usually focused on the anti-pathogen role of S...

Chilling injury reduces the quality and commercial value of banana. The effects of exogenous phytosulfokine α (PSKα) on chilling tolerance of banana via modulating the metabolism of polyamine, proline, γ-aminobutyric acid (GABA) were studied. The results showed that the chilling injury of banana was inhibited by 150 nM PSKα treatment, which was 13....

Foodborne pathogen contamination in horticulture crops has become a serious concern to human health. Several methods for detecting foodborne pathogens in horticulture crops were employed to manage and anticipate these pathogens. Convectional techniques for foodborne pathogens detection have some disadvantages, including time-consuming, low sensitiv...

Primary metabolites play crucial roles during fruit development and ripening. Phytohormone abscisic acid (ABA) has been reported to regulate postharvest tomato fruit ripening. However, knowledge about ABA in regulating primary metabolism in tomato fruit is obscure. In the present research, cherry tomato fruit were infiltrated with 1.0 mM ABA or dei...

Suberin polyphenolic (SPP), a kind of agronomically significant biopolymer, is composed primarily of phenolic acids generally synthesized by phenylpropanoid metabolism. In this study, the transcriptional activation of suberin polyphenolic biosynthesis by jasmonic acid (JA) inducible-transcription factor AchMYC2 was confirmed in wound tissues of kiw...

Pathogens are one of the supreme threats for the public health around the world in food supply chain. The on-site monitoring is an emerging trend for screening pathogens during the food processing and preserving. Traditional analytical tools have been unable to satisfy the current demands. Smartphones have enormous potentials for achieving on-site...

The impact of red and blue lightemitting diodes (LEDs) irradiation on the sugar metabolism and γaminobutyric acid in postharvest table grapes stored at (4 ℃) for 21 d after harvest were explored. Grapes fruit were exposed to red and blue light irradiation at the same intensity (500 LUX). The findings revealed that red and blue light treatments enha...

The influence of red light-emitting diodes (LEDs) irradiation (454 LUX) on the quality and physiology of postharvest table grapes fruit stored at (22.5 ± 2.5 ℃) for 6 days after harvest were explored. The results exhibited that red-light irradiation exposure was remarkably efficient in decreasing the weight loss of the fruit. The rachis browning an...

Camellia oleifera shells are a noteworthy by-product often considered as agricultural waste and discarded rather than efficiently used. The extraction of bioactive compounds from shells using green extraction process can be a better approach for waste valorization. Also, demand for novel and green extraction method is required to avoid organic solv...

In present work, green and efficient glycerol solvent system was coupled with pulse-probe ultrasonication for one-step extraction and preservation of anthocyanin from blueberry pomace. Under optimal conditions (40 min, 174 W, 18.6 mL/g, 20% of glycerol fraction), extraction yield was 23.07 ± 0.09 mg C3GE/g DW. The extracted anthocyanins were charac...

Background With the rising demand of microbiologically and chemically safe food products, conventional chemical preservatives are gradually fading away from the interests of both researchers and consumers. Thus, natural products are considered as promising substitution, especially botanical volatile organic compounds (VOCs) with bio-compatibility,...

The fast and precise point-of-care quantification of pathogens is an effective way to monitor and control diseases. In this work, we report a digital recombinase polymerase amplification (dRPA) in hydrogel nanofluidic chip for absolute quantification of nucleic acids as short as 5 min, without the need for complex chip operation. To perform the hyd...

Economic value of the global potato harvest is impacted by sprouting during storage. We examined how sprouting might be reduced or eliminated using citral, a naturally occurring component in citrus fruit peel. The current study integrated both loading and sustained release of citral using emulsification-based interfacial synthesis of hollow MIL-88A...

Fungicides are often used to extend the storage time of postharvest satsuma mandarin fruit. In recent years, fungicide residue has become an issue of food safety. This study aimed to investigate the distribution and migration of three typical fungicides (imazalil, prochloraz, thiophanate-methyl) in postharvest satsuma mandarins using liquid chromat...

Anthocyanins have received considerable attention for the development of food products with attractive colors and potential health benefits. However, anthocyanin applications have been hindered by stability issues, especially in the context of complex food matrices and diverse processing methods. From the natural microenvironment of plants to compl...

Owing to the high desorption energy of colloidal particles, Pickering emulsions can effectively protect the encapsulated bioactives. In this study, zein and peach gum polysaccharide (PGP) based biopolymeric nanoparticles were fabricated to stabilize the Pickering emulsion and deliver curcumin. Effects of the PGP addition order during and after anti...

Light exposure could trigger a series of physiological progress in strawberry and influence the commercial values. In the present study, the red-light irradiation promoted the red coloration of postharvest strawberry, combined with the doubled content of pelargonidin-based anthocyanins. Alternatively, no significant difference was found in cyanidin...

Main conclusion Exogenous ABA played a positive role in the accumulation and biosynthesis of aroma components of postharvest kiwifruit after low-temperature storage, especially the esters production during ripening. Abstract Low-temperature storage (LTS) generally affects the aroma formation associated with the decrease in aroma quality in kiwifru...

The multiple functions of peptides released from proteins have immense potential in food and health. In the past few decades, research interest in bioactive peptides of plant origin has surged tremendously, and new plant-derived peptides are continually discovered with advances in extraction, purification, and characterization technology. Plant-der...

In this work, the soy protein/chitosan-based microsphere (SCM), as a stable biocompatible vehicle of oleanolic acid (OA), was constructed for the first time under acidic conditions, and OA was firstly used for quality maintenance of minimally processed products. The effect of soybean protein isolate/chitosan ratio, particle concentration and oil fr...

Melatonin (MLT) exhibited pleiotropic effects in multiple plant physiological processes; however, its role in the polyamine metabolism and quality performance of postharvest Vitis vinifera berry remain unknown. In the present study, MLT was applied to postharvest Vitis vinifera cv. Kyoho berry, which delayed berry abscission, berry rotten, and prom...

Methyl jasmonate (MeJA) acts as an elicitor to stimulate phenolic compounds in fruit and vegetables. To explore the regulation of MeJA on phenolic compounds biosynthesis during postharvest tomato ripening, mature green tomato fruit were treated by 0.5 mM MeJA or deionized water under vacuum, and then kept in darkness for 16 d. Total phenolics and t...

Pesticides are indispensably essential in agricultural practices. Traditional pesticides and environmentally friendly pesticides both are used to control the damage caused by pests and diseases. Conventional procedures were carried out to detect pesticide residues using chromatography and spectrophotometric techniques. Innovative extractions (micro...

Chilling injury occurs in banana fruit under cold storage, which decreases commercial value and limits the shelf-life. Here, exogenous epibrassinolide was applied to investigate its regulation on chilling tolerance. The results found 2.5 μmol/L epibrassinolide was the optimal concentration to reduce chilling injury, which showed 23% lower chilling...

This study proposed natural deep eutectic solvent (NADES) based pulse-ultrasonication-assisted extraction (P-UAE) for green recovery of crocins from gardenia fruits (Gardenia jasminoides Ellis). Choline chloride-1,2-propylene glycol (ChCl-PG, 1:2.5, mol/mol) with 35 % (v/v) water content was tailor-made to obtain the highest extraction efficiency w...

Nicotinamide adenine dinucleotide (NAD), a vital energy metabolite, functions as a signal substance in plant immunity, which has been emerged as a 5'-terminal capping of RNA. In response to gray mold disease, the association among NAD decapping (deNAD), fruit immunity and energy status was studied in strawberry. Obvious necrosis was observed at 3 d...

Background Our previous study has demonstrated that the transcription of AchnKCS involved in suberin biosynthesis was up-regulated by exogenous abscisic acid (ABA) during the wound suberization of kiwifruit, but the regulatory mechanism has not been fully elucidated. Results Through subcellular localization analysis in this work, AchnbZIP29 and Ac...

During postharvest life, nitric oxide synthase/nitric oxide (NOS/NO) and phytosulfokine α/phytosulfokine receptor 1 (PSKα/PSKR1) systems activity promote cytosolic guanosine 3′, 5′-cyclic monophosphate (cGMP) accumulation in fruits and vegetables. Protein kinases G (PKGs), cyclic nucleotide-gated ion channels (CNGCs), and phosphodiesterases (PDEs)...

Spontaneous and continuous collection of water energy from the natural environment provides an ingenious alternative for green electricity technologies. However, power generation using hygroscopic materials usually decays quickly in a...

Modified atmosphere packaging (MAP) has been widely known to delay the postharvest fruit senescence; nevertheless, its effect on antioxidant activity and cell wall metabolism of wolfberry fruit is largely unknown. The present study investigated the impact of elevated CO2 on the quality attributes and cell wall degradation of wolfberry fruit during...

Melatonin, a tryptophan-derived molecule, is endogenously generated in animal, plant, fungal and prokaryotic cells. Given its antioxidant properties, is involved in a myriad of signalling functions associated with various aspects of plant growth and development. In higher plants, melatonin interacts with plant regulators such as phytohormones, as w...

Elevated CO2 shows adverse effects in horticultural crops including off-flavor formation and carbohydrate consumption. Here, 1 mM adenosine triphosphate (ATP) was applied to strawberry fruit under 20 % CO2 atmosphere to investigate its regulation on fermentative and carbohydrate metabolism. The results showed that ATP treatment increased endogenous...

Raw peach gum is hard crystalline-like solid with very poor solubility, significantly restricting its application in various fields. This study optimized the parameters for ultrasonic-assisted peach gum polysaccharides (UPGP) extraction. The results showed that the maximum extraction rate was up to 80.1% under the optimized conditions of 5 h extrac...

The spatial distribution and time-course evolution of polyphenol compounds in response to exogenous light and temperature in grape berry (Vitis labruscana cv. ‘Kyoho’) were examined. Results demonstrated that the polyphenol biosynthesis was induced via the up-regulated gene transcription levels during berry ripening. The polyphenol biosynthesis was...

Sucrose metabolism is a fundamental process during tomato ripening. Studies have shown the role of the phytohormone methyl jasmonate (MeJA) in tomato fruit ripening. However, the role of MeJA in regulating sucrose metabolism in tomato is still unclear. In this study, mature green cherry tomato fruit were infiltrated with MeJA (0.5 mM) or sterile de...

DNA Origami-Based Emulsion An amphiphilic triangular DNA origami successfully attaches onto the water-in-oil and oil-in-water interfaces to solely stabilize the W/O/W emulsion. The DNA origami-based emulsion is functionalized as a material platform for a bind-and-release of specific cargos. More information can be found in article 2104831 by Xingyu...

In this work, instead of performing nucleic acid amplification in the bulk solution, we report a nanoporous hydrogel with controlled release function for rapid, precise, and inhibition-free nucleic acid analysis in crude food samples. The cross-linked PEG hydrogel with nanoporous structures possesses adsorption, release, separation, restriction and...

Programmable engineered DNA origami provides infinite possibilities for customizing nanostructures with controllable precision and configurable functionality. Here, a strategy for fabricating an amphiphilic triangular DNA origami with a central nanopore that integrates phase-stabilizing, porous-gated, and affinity-delivering effects is presented. B...

Because of high sensitivity and specificity, isothermal nucleic acid amplification are widely applied in many fields. To facilitate and improve their performance, various nanomaterials, like nanoparticles, nanowires, nanosheets, nanotubes, and nanoporous films are introduced in isothermal nucleic acid amplification. However, the specific applicatio...

In this paper, the gas-assisted combined with glycerol extraction (GAGE) for polyphenol recovery from lotus seedpods (LSPs) was modeled and optimized. Box–Behnken design was applied to optimize the total polyphenol content (TPC) of LSP along with enhancing antioxidant activities using response surface methodology based on the TPC extraction yield (...

Shiitake mushroom (Lentinus edodes) is one of the most precious edible mushrooms. It has a variety of nutritional and bioactive compounds consumed as both food and medicine. Several preservation approaches were applied to avoid the short shelf-life of fresh shiitake mushroom. The present review aimed to comprehensively discuss the latest developmen...

Natural deep eutectic solvent (NADES) has received increasing interest as a green alternative to traditional organic solvents for efficient extraction of bioactive compounds from natural sources. In this study, phytochemicals in Carya cathayensis Sarg. peels extracted with Choline chloride-Malic acid (ChCl-MA) were identified using UPLC-Triple-TOF/...

The hybrid oxidation effect of ultrasonics (US) and ultraviolet light (UV-A) on Cyanidin-3-O-glucoside (C3G) stability were examined. For comparison, sonolysis and photolysis experiments were also conducted. The results showed that under hybrid sonophotolysis and sonolysis treatment, C3G degradation undergoes zero-order kinetics, while under photol...

Aroma is a vital characteristic that determines the quality and commercial value of fruit. Fatty acids (FAs) are key precursors for fruit aroma volatiles. In Arabidopsis thaliana, TT2/MYB123 inhibited the seed embryo FA biosynthesis. We analyzed the function and regulation of the MYB123 homolog (Fragaria × ananassa [Fa] MYB11) for FA metabolism, es...

Nanobubbles (NBs) generated-nanojets membrane poration have gained enormous attention. In this study, NBs were fabricated as a novel green approach to assist ionic liquid (IL) [C4C1im][BF4] extraction of polyphenols from Carya cathayensis Sarg. husk. NBs were successfully generated with mean size of 85.47 ± 5 nm, zeta potential of +39 ± 2.24 mV, an...

Sphingolipids (SLs) are common in all eukaryotes, prokaryotes, and viruses, and played a vital role in human health. They are involved in physiological processes, including intracellular transport, cell division, and signal transduction. However, there are limited reviews on dietary effects on endogenous SLs metabolism and further on human health....

In this study, the mechanism by which the exogenous application of 150 nM signaling bioactive peptide phytosulfokine α (PSKα) delays senescence and improves antioxidant nutrient accumulation in strawberry fruit during storage at 4 °C for 18 d was investigated. Results showed that the higher endogenous accumulation of PSKα in strawberry fruit treate...

In this work, we developed a facile, ultrafast, and solvent-free polydimethylsiloxane (PDMS) coating method triggered by plasma for ultrathin coating on various nanostructured substrates at room temperature. During plasma treatment, the monomers in the bulk elastomer were excited and deposited on the surrounding substrates. The whole coating proces...

Fresh-cut pear are popular for their nutritive, fresh and convenience. However, the oxidative stress caused by wounding aggravates quality loss. In the present study, the effects of 10 % CO2 on reactive oxygen species (ROS) metabolism and antioxidant capacity in fresh-cut pear were investigated. The results showed that 10 % CO2 effectively inhibite...

Enzymatic browning is a major problem of fresh-cut pear, which severely limits the shelf life. In the current study, elevated carbon dioxide (CO2) was applied to investigate its effects on quality maintenance and browning repression. The results showed that 5% and 10 % CO2 treatment maintained quality attributes, including firmness, color, and tota...

Investigation of plant‐fungal pathogen interaction is one of the most interesting fields in plant sciences. However, the roles of plant volatile organic compounds in the arms race are still largely unknown. Based on precise quantification of plant volatiles, we discovered that the plant volatile organic compound (E)‐2‐hexenal, at concentrations tha...

Raman spectroscopy and surface‐enhanced Raman spectroscopy (SERS) have been extensively explored in the design of accurate, transparent, and conclusive food safety and quality control assays. Its hyphenation with chemometric algorithms is instrumental in securing safe food campaigns. To provide valuable recommendations and meet the growing demands...

In the present study, a novel electrochemical sensor for the direct detection of caffeine in the crude sample has been prepared by plasma-triggered polydimethylsiloxane (PDMS) deposition on the indium tin oxide electrodes supported with silica nanochannels. The deposited PDMS contains both the original hydrophobic and oxidized hydrophilic PDMS olig...

Phenolic compounds are minor metabolites usually present in mushroom species. Because of their potential advantages for human health, such as antioxidant and other biological activities, these bioactive components have been gaining more interest as functional foods, nutraceutical agents for providing better health conditions. This review aims to co...

Major losses of fresh horticultural produce transpire during postharvest storage due to prompt senescence and diseases. The traditional postharvest preservation techniques used after harvest are based on cooling and the application of chemical preservation techniques. As a residue-free physical sterilization and preservation method, light-emitting...

To investigate the effects of exogenous melatonin (MLT) treatment on the quality of postharvest jujubes, fresh ‘Lingwuchangzao’, and ‘Dongzao’ jujubes (Zizyphus jujuba Mill) were dipped in MLT solution at the dose of 0, 50,100 and 200 μmol L⁻¹ for 20 min. Results showed the exogenous MLT application significantly delayed the color change and firmne...

Keywords: Phase change materials P. eryngii Amino acid metabolism Energy metabolism ABSTRACT: Phase change material (PCM) is a substance which absorbs latent heat by a phase transition, and it plays important roles in short-time cold chain transportation. In the present study, we developed a novel water-based PCM, which showed a better effect on...

Plant cuticular wax is the first barrier to resist biotic and abiotic stress. However, little is known about the compositional differences between the epicuticular and intracuticular wax in grape berry. The compositional, morphological and functional features of cuticular wax in grape berries of Vitis vinifera cv. ‘Kyoho’, ‘Muscat Hamburg’, ‘Redglo...

A deep understanding upon the relation between anthocyanin extraction performance and physicochemical properties of natural deep eutectic solvents (NADESs) was obtained, and a high-efficiency NADES (Choline chloride-Oxalic acid (ChOa)) was selected and coupled with pulse-ultrasonication for anthocyanin extraction from blueberry pomace. Response sur...

An excellent high-efficiency natural deep eutectic solvent (NADES, ChCl-MA) was screened out and integrated with pulse-ultrasonication technique for extracting phenolic compounds from Carya cathayensis Sarg. peels (CCSPs). Single factor experiment combined with response surface methodology (RSM) using Box-Behnken design (BBD) were employed to inves...

Direct electrochemical detection in real food samples remains challenging due to the fouling and interference by abundant interference components. Herein, we report an electrochemical sensing platform based on binary assembly of silica nanochannels and polydimethylsiloxane that is able to detect Pb²⁺ and Cd²⁺ in real food samples without complex pr...