Zhugen Yang

Zhugen Yang
Cranfield University · School of Water, Energy and Environment

NERC Fellow MC Fellow, FHEA

About

71
Publications
16,576
Reads
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2,364
Citations
Introduction
Academic staff at Cranfield University (UK) focuses on the development of biological and chemical sensors, and paper-microfluidics devices for point-of-use diagnosis of water quality, environment and human health. Research webpage: www.zhugenyang.com
Additional affiliations
October 2015 - March 2019
University of Glasgow
Position
  • Lecturer-RA
Description
  • We developed paper-based device for point-of-care diagnosis of infectious diseases.
October 2013 - October 2015
University of Bath
Position
  • Marie Curie Fellow
October 2012 - October 2013
University of Cambridge
Position
  • PostDoc Position
Education
September 2009 - July 2012
Ecole Centrale de Lyon
Field of study
  • Micro and nanotechnology
September 2006 - July 2009
Sun Yat-Sen University
Field of study
  • Polymer Chemistry and Physics
September 2002 - July 2006
Harbin Institute of Technology
Field of study
  • Polymer Materials and Engineering

Publications

Publications (71)
Article
Full-text available
We report a rapid “sample-to-answer” platform for the quantitative monitoring of genetic biomarkers within communities by analysis of wastewater. The assay is based on the loop-mediated isothermal amplification (LAMP) and shows for the first time the ability to rapidly quantify human-specific mitochondrial DNA (mtDNA) from raw untreated wastewater...
Article
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The health and well-being of cattle is a significant concern for global agricultural output. In dairy production within low and middle income countries (LMICs), there is a significant biosensing challenge in detecting sexually transmitted infection (STI) pathogens during animal husbandry, due in part to difficulties associated with the limited infr...
Article
Full-text available
Rapid, low-cost, species-specific diagnosis, based upon DNA testing, is becoming important in the treatment of patients with infectious diseases. Here, we demonstrate an innovation that uses origami to enable multiplexed, sensitive assays that rival polymerase chain reactions (PCR) laboratory assays and provide high-quality, fast precision diagnost...
Article
Full-text available
The early diagnosis of active hepatitis C virus (HCV) infection remains a significant barrier to the treatment of the disease and to preventing the associated significant morbidity and mortality seen, worldwide. Current testing is delayed due to the high cost, long turnaround times and high expertise needed in centralised diagnostic laboratories. H...
Article
Full-text available
In type II CRISPR systems, the guide RNA (gRNA) comprises a CRISPR RNA (crRNA) and a hybridized trans-acting CRISPR RNA (tracrRNA), both being essential in guided DNA targeting functions. Although tracrRNAs are diverse in sequence and structure across type II CRISPR systems, the programmability of crRNA-tracrRNA hybridization for Cas9 is not fully...
Chapter
Nose-to-brain drug delivery system is becoming a desirable alternative approach to conventional drug delivery systems used for the treatment of various neurological disorders. Trigeminal and olfactory routes are implicated to deliver drugs from the nose-to-brain, which bypasses the blood-brain barrier and the first-pass metabolism. In this review,...
Article
Full-text available
Conventional techniques (e.g., culture-based method) for bacterial detection typically require a central laboratory and well-trained technicians, which may take several hours or days. However, recent developments within various disciplines of science and engineering have led to a major paradigm shift in how microorganisms can be detected. The analy...
Article
Graphdiyne quantum dots (GDQDs) have attracted increasing attentions due to its unique electronic, optical, and electrochemical properties. However, the low conductivity and quantum yield of GDQDs limit their application. Here, nitrogen-doped graphdiyne dots (N-GDQDs) are firstly synthesized by a simple, friendly and one-step hydrothermal method. T...
Article
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Corona Virus Disease 2019 (COVID-19) has developed into a global pandemic in the last two years, causing significant impacts on our daily life in many countries. Rapid and accurate detection of COVID-19 is of great importance to both treatments and pandemic management. Till now, a variety of point-of-care testing (POCT) approaches devices, includin...
Article
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A review of the current status of direct and indirect potable water reuse (DPR/IPR) implementation has been conducted, focusing on the regulatory and practical aspects and with reference to the most recent published literature. The review encompasses (a) the principal contaminant types, their required removal and the methods by which their concentr...
Article
We prepared a biocompatible AuAg nanocages/[email protected] polyethylene glycol (AuAg/[email protected]) composite. The combination of AuAg and GDY to obtain a synergistically enhanced photothermal effect, and the antibacterial effect of GDY and AuAg are used in combined anti-infective therapy. The in vitro antibacterial activity of AuAg/[email pr...
Article
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Environmental contaminants are a global concern, and an effective strategy for remediation is to develop a rapid, on-site, and affordable monitoring method. However, this remains challenging, especially with regard to the detection of various contaminants in complex water environments. The application of molecular methods has recently attracted inc...
Article
Viruses are omnipresent and persistent in wastewater, which poses a risk to human health. In this review, we summarize the different qualitative and quantitative methods for virus analysis in wastewater and systematically discuss the spatial distribution and temporal patterns of various viruses (i.e., enteric viruses, Caliciviridae (Noroviruses (No...
Article
Infectious diseases caused by pathogenic microbes have posed a major health issue for the public, such as the ongoing COVID-19 global pandemic. In recent years, wastewater-based epidemiology (WBE) is emerging as an effective and unbiased method for monitoring public health. Despite its increasing importance, the advancement of WBE requires more com...
Article
Full-text available
Paper-based nanosensors to evaluate community-wide illicit drug use for wastewater-based epidemiology , Water Research (2020), doi: https://doi. Highlights • Au@Ag core-shell nanoparticles for high sensitivity SERS assay • Fabrication of Au@Ag-pLL on glass nanofibrous paper as SERS substrate • A paper-based SERS nanosensor for the sensitive detecti...
Article
Full-text available
Public health is attracting increasing attention due to the current global pandemic, and wastewater-based epidemiology (WBE) has emerged as a powerful tool for monitoring of public health by analysis of a variety of biomarkers (e.g., chemicals and pathogens) in wastewater. Rapid development of WBE requires rapid and on-site analytical tools for mon...
Article
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Antimicrobial resistance (AMR) in the environment is a global concern for public health and recent studies have showed that various soil pollutants (e.g. heavy metals, petroleum hydrocarbons) can cause the emergence of antibiotic-resistant bacteria and antibiotic-resistance genes in soil. This emergence of AMR in soil is therefore prompting the res...
Article
Full-text available
Coronavirus disease (COVID-19), caused by SARS-CoV-2, evolved into a global pandemic in 2020, and the outbreak has taken an enormous toll on individuals, families, communities and societies around the world. One practical and effective strategy is to implement rapid case identification based on a rapid testing to respond to this public health crisi...
Article
The determination of contaminants of emerging concern (CECs) in environmental samples has become a challenging and critical issue. The present work focuses on miniaturized analytical strategies reported in the literature for the determination of CECs. The first part of the review provides brief overview of CECs whose monitoring in environmental sam...
Article
Point-of-use sensors Wastewater-based epidemiology Mobile health Pandemic Early warning A B S T R A C T The outbreak of coronavirus disease (COVID-19) has caused a significant public health challenge worldwide. A lack of effective methods for screening potential patients, rapidly diagnosing suspected cases, and accurately monitoring of the epidemic...
Article
Full-text available
Graphdiyne (GDY) as an emerging carbon nanomaterial has attracted increasing attention because of its uniformly distributed pores, highly π-conjugated, and tunable electronic properties. These excellent characteristics have been widely explored in the fields of energy storage and catalysts, yet there is no report on the development of sensors based...
Article
Heavy metal/metalloids (HMs) and polycyclic aromatic hydrocarbons (PAHs) in soil have caused serious environmental problems, compromised agriculture quality, and have detrimental effects on all forms of life including humans. There is a need to develop appropriate and effective remediation methods to resolve combined contaminated problems. Although...
Article
Rice is the leading staple food for more than half of the world’s population, and approximately 160 million hectares of agricultural area worldwide are under rice cultivation. Therefore, it is essential to fulfil the global demand for rice while maintaining food safety. Rice acts as a sink for potentially toxic metals such as arsenic (As), selenium...
Article
For in-situ disease markers detection, point-of-care (POC) diagnosis has great advantages in speed and cost compared with traditional techniques. The rapid diagnosis, prognosis, and surveillance of diseases can significantly reduce disease-related mortality and trauma. Therefore, increasing attention has been paid to the POC diagnosis devices due t...
Article
Full-text available
The abuse of illicit drugs usually associated with dramatic crimes may cause significant problems for the whole society. Wastewater-based epidemiology (WBE) has been demonstrated to be a novel and cost-effective way to evaluate the abuse of illicit drugs at the community level, and has been used as a routine method for monitoring and played a signi...
Article
Cadmium (Cd) is one of the most harmful and widespread environmental pollutants. Despite decades-long research efforts, the remediation of water contaminated by Cd has remained a significant challenge. A novel carbon material, pinecone biochar, was previously hypothesized to be a promising adsorbent for Cd, while so far, it has received little atte...
Article
Full-text available
Biosensors and nanoscale analytical tools have seen unprecedented growth in literature in the past 20 years, with a large number of reports on the topic of ’ultra-sensitive’, ’cost-effective’ and ’early-detection’ tools with a potential of ’mass- production’ cited on the web of science. Yet none of these tools are commercially available in the mark...
Article
Full-text available
In this work, an electrochemical paper-based aptasensor was fabricated for label-free and ultrasensitive detection of epidermal growth factor receptor (EGFR) by employing anti-EGFR aptamers as the bio-recognition element. The device used the concept of paper-folding, or origami, to serve as a valve between sample introduction and detection, so redu...
Article
This review highlights arsenic (As) and selenium (Se) sources in the environment, their uptake in the soil-plant system, interactions between these metals and the associated toxicity in major biological compartments, which may assist in addressing the hazardous impacts associated with As and Se contamination. The interaction between As and Se is a...
Article
Paper is a common material that is promising for constructing microfluidic chips (lab-on-a-paper) for diagnostics and drug delivery for biomedical applications. In the past decade, extensive research on paper-based microfluidics has accumulated a large number of scientific publications in the fields of biomedical diagnosis, food safety, environment...
Article
Wastewater-based epidemiology (WBE) was applied for the first time in seven cities across Europe with the aim of estimating quinolones consumption via the analysis of human urinary metabolites in wastewater. This report is also the first pan-European study focussed on the enantiomeric profiling of chiral quinolones in wastewater. By considering loa...
Article
Mercury (Hg) bioaccumulation in rice poses a health issue for rice consumers. In rice paddies, selenium (Se) can decrease the bioavailability of Hg through forming the less bioavailable Hg selenides (HgSe) in soil. Rice leaves can directly uptake a substantial amount of elemental Hg from the atmosphere, however, whether the bioaccumulation of Hg in...
Article
Auxetic materials with negative Poisson's ratios unusually exhibit intuitive mechanical behaviors, such as cross-section expansion instead of contraction during tension. Such behaviors are interesting because they may enhance unusual mechanical properties. However, controllable preparation of materials with negative Poisson's ratio is still a major...
Article
Arsenic (As) is a highly toxic contaminant in the environment and a serious carcinogen for the human being. The toxicity of arsenic significantly threatens environmental and human health. The effective removing technology for arsenic remains challenging, and one of the reasons is due to the lack of powerful detection method in the complex environme...
Article
The abuse of illicit drug addiction is a worldwide public health and social problem. In this paper, we reported on a simple and rapid colorimetric biosensor for duplexed detection of methamphetamine (METH) and cocaine in a single assay. Gold nanoparticles (AuNPs) and Au@Ag NPs were synthesized and functionalized with DNA reporter probes (RPs) for M...
Article
Wastewater-based epidemiology (WBE) has been shown to be an innovative approach for evaluation of drug use trends and public health assessment by quantifying drug residues and/or metabolites (so-called biomarkers) in wastewater collected in a local treatment plant. Community sewage sensors have been proposed and demonstrated to be powerful tool for...
Article
Full-text available
Prostate specific antigen (PSA) is a widely used marker for the diagnosis of prostate cancer, and the increasing attention has been attracted on the development of rapid assay using biosensing technology. However, it remains challenging for the sensitive and selective detection of PSA in clinical samples. Here, we report a label-free microfluidic p...
Article
Full-text available
We describe a novel biosensing strategy for sensitive detection of methylamphetamine (MAMP) based on surface enhanced Raman scattering (SERS) by the mediation of spacing between 4-mercaptobenzoic acid (4-MBA) labeled [email protected] core-shell nanoparticles ([email protected]). To achieve a favorable SERS substrate, [email protected] shell-core n...
Article
Caffeine metabolites in wastewater were investigated as potential biomarkers for assessing caffeine intake in a population. The main human urinary metabolites of caffeine were measured in the urban wastewater of ten European cities and the metabolic profiles in wastewater were compared with the human urinary excretion profile. A good match was foun...
Article
The aim of this paper is to present the first study on spatial and temporal variation in the enantiomeric profile of chiral drugs in eight European cities. Wastewater-based epidemiology (WBE) and enantioselective analysis were combined to evaluate trends in illicit drug use in the context of their consumption vs direct disposal as well as their syn...
Article
We report a novel colorimetric biosensor based on non-aggregation Au@Ag core-shell nanoparticles to detect methamphetamine and cocaine. The biosensor consisted of a reporter probe (RP) that is a specific single-stranded DNA (ssDNA) sequence coated on Au@Ag nanoparticles, a capture probe (CP) conjugated with magnetic beads, and an illicit drug-bindi...
Article
Human biomonitoring, i.e. the determination of chemicals and/or their metabolites in human specimens, is the most common and potent tool for assessing human exposure to pesticides, but it suffers from limitations such as high costs and biases in sampling. Wastewater-based epidemiology (WBE) is an innovative approach based on the chemical analysis o...
Article
The information obtained from the chemical analysis of specific human excretion products (biomarkers) in urban wastewater can be used to estimate the exposure or consumption of the population under investigation to a defined substance. A proper biomarker can provide relevant information about lifestyle habits, health and wellbeing, but its selectio...
Article
Full-text available
Illicit drug use has a global concern and effective monitoring and interventions are highly required to combat drug abuse. Wastewater-based epidemiology (WBE) is an innovative and cost-effective approach to evaluate community-wide drug use trends, compared to traditional population surveys. Here we report for the first time, a novel quantitative co...
Article
In this work, a simple, cost-effective, and label-free biosensor was constructed for methamphetamine (METH) detection. The biosensor consists of a G-quadruplex-hemin DNAzyme molecular beacon (DNAzyme MB), a METH aptamer, and a colorimetric substrate. The DNAzyme MB loses peroxidase activity when it hybridizes with the METH aptamer. In the presence...
Article
A new label-free electrochemical DNA (E-DNA) biosensor using a custom synthesized ferrocenyl (Fc) double-stranded DNA (dsDNA) intercalator as a redox marker is presented. Single-stranded DNA (ssDNA) was co-immobilized on gold electrodes with 6-mecarpto-hexanol (MCH) to control the surface density of the ssDNA probe, and hybridized with complementar...
Article
Full-text available
We report on a novel strategy for DNA aptamer immobilization to develop sensitive electrochemical detection of a protein biomarker, with prostate specific antigen (PSA) as a case biomarker. Thiolated single-stranded DNA (ssDNA) was co-immobilized with 3-mercapto-1-propanol on gold electrodes, and used as a scaffold for DNA aptamer attachment throug...
Book
Full-text available
Protein microarrays have shown a powerful tool to identify cancer biomarkers due to the multiplex detection and tiny sample requirement. However, there is no single biomarker that is sensitive and specific enough to meet strict diagnostic criteria due to the diversity and variation of cancers. To this end, a combination of biomarkers is required to...
Conference Paper
Full-text available
A wide diversity of serological biomarkers is available for clinic diagnosis but individually their lack of specificity decreases their diagnostic and prognostic values. Thus, there is an urgent need to develop more sensitive, reliable and specific screening tools for biomarkers detection. Protein microarrays have demonstrated to be powerful for sc...
Article
Antibody microarrays are powerful and high-throughput tools for screening and identifying tumor markers from small sample volumes of only a few microlitres. Optimization of surface chemistry and spotting conditions are crucial parameters to enhance antibodies immobilization efficiency and to maintain their biological activity. Here, we reported the...
Article
Protein chips have demonstrated to be a sensitive and low cost solution to identify and detect tumor markers. However, efficient multiparametric analysis remains a challenge due to protein variability. Crucial parameters are the design of stable and reproducible surfaces which maintain biological activity of immobilized proteins. These parameters r...
Article
There is an urgent need to identify relevant tumor markers showing high sensitivity and specificity for early diagnosis and prognosis of breast cancer. Protein microarrays have demonstrated to be cost-effective, high through-put and powerful tools for screening and identifying tumor markers with only minute samples. Autoantibodies directed against...
Article
Full-text available
Core shell Ag@SiO 2-Streptavidin-Cy3 nanoparticles were prepared. Ag@SiO 2 nanoparticles were synthesized via a solgel method. Then, Streptavidin-Cy3 was covalently bonded to the Ag@SiO 2 surface. These core-shell nanoparticles were characterized by steady-state fluorescence spectroscopy and fluorescence scanning. In presence of the silver core, a...
Article
Full-text available
Protein chips have demonstrated to be a sensitive and low cost solution to identify and detect tumor markers. However, efficient multiparametric analysis remains a challenge due to protein variability. Crucial parameters are the design of stable and reproducible surfaces which maintain biological activity of immobilized proteins, and immobilization...
Article
A highly novel nano-CaCO(3) supported beta-nucleating agent was employed to prepare beta-nucleated isotactic polypropylene (iPP) blend with polyamide (PA) 66, beta-nucleated iPP/PA66 blend, as well as its compatibilized version with maleic anhydride grafted PP (PP-g-MA), maleic anhydride grafted polyethylene-octene (POE-g-MA), and polyethylene-viny...
Article
β-Nucleated isotactic polypropylene (β-iPP) blend with maleic anhydride grafted polyethylene-vinyl acetate (EVA-g-MA) and β-iPP/polyamide (PA) 6 blend, as well as its compatibilized version with EVA-g-MA as a compatibilizer were prepared with an internal mixer. Analysis from differential scanning calorimeter (DSC) and wide angle X-ray diffraction (...
Article
BACKGROUND: The distribution of nucleating agents in different phases is still an open question in general, and how to control conditions to prepare alloys rich in beta-crystals of polypropylene (PP) is hardly reported. The main goal of this study was to find out the factors influencing the beta-crystal content in beta-nucleated PP/polyamide 6 (PA6...
Article
beta-Nucleated isotactic polypropylene (iPP) blends with polyamide 6 (PA6) were prepared with a novel and highly efficient nano-CaCO(3)-supported beta-nucleating agent. Maleic anhydride grafted polypropylene (pp-g-MA), glycidyl methacrylate grafted polypropylene (PP-g-GMA), maleic anhydride grafted poly(ethylene octene) (POE-g-MA), and maleic anhyd...
Article
Non-nucleated polypropylene alloy with polyamide 6 (PP/PA6) and β-nucleated polypropylene (β-PP)/PA6 alloy, as well as its compatibilized version with maleic anhydride grafted PP (PP-g-MA) were prepared with an internal mixer. In the all alloys, PP formed a continuous phase with a dispersive PA6. Effects of PA6 on the non-isothermal crystallization...