Zhongren Ding

Fudan University

Coronary artery disease (CAD) is characterized by the chronic immune-inflammation, excessive endoplasmic reticulum (ER) stress, and platelet hyperactivity; however, whether there is a signaling hub linking these events remains unclear. Here, we identified that triggering receptor expressed on myeloid cells 2 (TREM2), an important pattern recognitio...

Arctium lappa L., also known as burdock, is a popular medicinal plant in traditional Chinese medicine due to its potential therapeutic properties. Saccharides from Arctium lappa L. root (ALR-S) have been extensively studied for their anti-inflammatory and anti-diabetes effects. Platelets play a pivotal role in thrombosis. The present study describe...

Aims Galectin-3, a β-galactoside-binding lectin, is abnormally increased in cardiovascular disease. Plasma Galectin-3 receives a Class II recommendation for heart failure management and has been extensively studied for multiple cellular functions. The direct effects of Galectin-3 on platelet activation remain unclear. This study explores the direct...

Background Platelets from septic patients exhibit increased reactivity. However, the underlying mechanism of sepsis-induced platelet hyperactivity is still not completely understood. Objective P2Y12 is a central receptor for platelet activation. In this study, we investigated the role of platelet P2Y12 in platelet hyperactivity during sepsis. Met...

Urine cells (or renal tubular cells) can be isolated from human urine samples efficiently. This noninvasive and cost-effective method to collect biological sample provide us favorable access to donor cells from human. In the present study, we generate ZZUNEUi022-A, a urine cells-derived induced pluripotent stem cell (iPSC) line, from urine sample o...

Background and Purpose Previously, we discovered that the activation of nucleotide-binding oligomerization domain 2 (NOD2) enhances platelet activation. We here investigated the antiplatelet and antithrombotic potential of GSK669, a NOD2 antagonist. Experimental Approach Effects of GSK669 on platelet functions, reactive oxygen species (ROS) and pr...

In this study, we report a human induced pluripotent stem cell (iPSC) line from a healthy 27-year-old female individual using non-integrative Sendai viral reprogramming technology. The cell line expresses stemness markers, exhibits a normal female karyotype, and can differentiate into three germ layers in vivo. This iPSC line from a healthy individ...

An induced pluripotent stem cell (iPSC) line was generated from peripheral blood mononuclear cells (PBMCs) of a 41-year-old male patient with hypertrophic cardiomyopathy who carries a G3755A heterozygote mutation in the MYH6 gene. The generated iPSC line expressed pluripotency markers, exhibited a normal karyotype, presented the specific mutation,...

Background: Proprotein convertase subtilisin/kexin 9 (PCSK9), mainly secreted by the liver and released into the blood, elevates plasma low-density lipoprotein (LDL) cholesterol by degrading LDL receptor. Pleiotropic effects of PCSK9 beyond lipid-metabolism have been shown. However, the direct effects of PCSK9 on platelet activation and thrombosis,...

Background: Critically ill patients diagnosed with COVID-19 may develop a pro-thrombotic state that places them at a dramatically increased lethal risk. Although platelet activation is critical for thrombosis and is responsible for the thrombotic events and cardiovascular complications, the role of platelets in the pathogenesis of COVID-19 remains...

Platelet activation plays a pivotal role in physiological hemostasis and pathological thrombosis causing heart attack and stroke. Previous studies conclude that simultaneous activation of Gi and G12/13 signaling pathways is sufficient to cause platelet aggregation. However, using Gq knockout mice and Gq-specific inhibitors, we here demonstrated tha...

Background: Platelets from patients with diabetes mellitus are hyperactive. Hyperactivated platelets may contribute to cardiovascular complications and inadequate responses to antiplatelet agents in the setting of diabetes mellitus. However, the underlying mechanism of hyperactivated platelets is not completely understood. Methods: We measured P...

Pattern recognition receptor nucleotide-binding oligomerization domain 2 (NOD2) is well investigated in immunity, but its expression and function in platelets has never been explored. Using reverse transcription polymerase chain reaction and Western blot, we show that both human and mouse platelets express NOD2, and its agonist muramyl dipeptide in...

Abstract Aberrant platelet activation plays a critical role in the pathogenesis of heart attack and stroke. dl-3-n-butylphthalide (NBP) has been approved in China to treat stroke with multiple mechanisms. The anti-stroke effects of NBP may be related to its antiplatelet effects reported in rats in addition to its antioxidative, antiapoptotic, and a...

P2Y12 receptor is a 342 amino acid Gi-coupled receptor predominantly expressed on platelets. P2Y12 receptor is physiologically activated by ADP and inhibits adenyl cyclase (AC) to decrease cyclic AMP (cAMP) level, resulting in platelet aggregation. It also activates PI3 kinase (PI3K) pathway leading to fibrinogen receptor activation, and may protec...

Background5,6‐Dimethylxanthenone‐4‐acetic acid (DMXAA) is a tumor vascular disrupting agent under clinical trials as an adjacent antitumor agent. DMXAA is structurally similar to flavone‐8‐acetic acid (FAA), an old tumor vascular disrupting agent with antiplatelet and antithrombotic effects. In contrast to FAA, which causes bleeding in tumor patien...

Many natural compounds derived from plants or microbes show promising potential for anticancer treatment, but few have been found to target energy-relevant regulators. In this study, we report that neoalbaconol (NA), a novel small-molecular compound isolated from the fungus, Albatrellus confluens, could target 3-phosphoinositide-dependent protein k...

Background: Platelet ADP receptor P2Y(12) is well studied and recognized as a key player in platelet activation, hemostasis and thrombosis. However, the role of P2Y(12) in platelet apoptosis remains unknown. Objectives: To evaluate the role of the P2Y(12) receptor in platelet apoptosis. Methods: We used flow cytometry and Western blotting to a...

Integrin αIIbβ3 mediated bidirectional signaling plays a critical role in thrombosis and haemostasis. Signaling mediated by the β3 subunit has been extensively studied, but αIIb mediated signaling has not been characterized. Previously, we reported that platelet granule secretion and TxA2 production induced by αIIb mediated outside-in signaling is...

PMA induced spreading of CHO cells expressing αIIbβ3-WT, αIIbβ3-scramble and αIIbβ3-Δ724. (A) In presence of 100 ng/ml PMA, spreading of CHO cells expressing αIIbβ3-WT, αIIbβ3-scramble and αIIbβ3-Δ724, respectively on immobilized Fg for 90 minutes. (B) Quantification of area (pixel number) in 4 random fields (mean ± SEM) all at 90 minutes. The size...

Background: In our previous in vitro study, we reported a constitutively active chimeric P2Y(12) (cP2Y(12)) and found that AR-C78511 is a potent inverse agonist at this receptor. The role of cP2Y(12) in platelet activation and thrombosis is not clear. Objectives: To investigate the physiologic implications of cP2Y(12) for platelet activation and...

In this study, a two-dimensional (2D) sinc interpolation method is proposed to carry out the geometric correction in highly squint synthetic aperture radar (SAR). The point spread function (PSF) has 2D non-perpendicular sidelobes in highly squint SAR. This kind of sidelobes may result in the folded skewed spectrum. The conventional interpolation me...

In this study, we report BF066, a novel adenine derivative, inhibits platelet activation and thrombosis via the adenosine receptor (A(2A)) activation and phosphodiesterase (PDE) inhibition. BF066 inhibits platelet aggregation and ATP releasing induced by multiple platelet agonists in a dose-dependent manner. The inhibition of BF066 on ADP-induced a...

PKA inhibitor PKI (14–22) inhibits forskolin-induced VASP phosphorylation in human platelets. Human washed platelets were preincubated with PKA inhibitor PKI (14–22) (12 µM) or BF066 (30 µM) for 15 min or 2 min at 37°C, respectively, followed by stimulation with forskolin (20 µM) or ADP (10 µM) for 3.5 min. VASP phosphorylation was detected via Wes...

BF066 prevents FeCl3-induced thrombosis in a mouse mesenteric arteriole. One bolus injection of BF066 (29 mg/kg, i.v.) 30 min before FeCl3 injury prevented thrombus formation in a C57BL/6 mouse mesenteric arteriole over 12 min. Platelets were labeled with calcein. (WMV)

SCH58261 does not antagonize the inhibition of BF061 on aggregation induced by ADP. A2A receptor antagonist SCH58261 (10 µM) did not block the inhibitory role of BF061 (10 µM) on ADP-induced platelet aggregation in aspirin-treated human washed platelets. As a control, SCH58261 almost abolished the inhibition of adenosine (10 µM) on platelet aggrega...

PKA inhibitors PKI (14–22) and H-89 do not reverse the inhibition of BF066 on ADP-induced platelet aggregation. Human washed platelets were preincubated with vehicle or PKA inhibitors PKI (14–22) (12 µM) and H-89 (19 µM) at 37°C for 18 min and 8 min, respectively, followed by incubation with BF066 (30 µM) or vehicle for another 1 min. Platelet aggr...

FeCl3–induced thrombosis in a mouse mesenteric arteriole. Multiple thrombi more than 30 µm were observed in the mesenteric arteriole at 5.5 min. At 12 min the arteriole was totally blocked by a stable bulky thrombus, occlusion occurred in all untreated mice within 14 min after FeCl3 injury Platelets were labeled with calcein. (WMV)

Clopidogrel prevents FeCl3-induced thrombosis in a mouse mesenteric arteriole. Clopidogrel (30 mg/kg, p.o., once daily for 2 days) prevented FeCl3-induced thrombus formation in a C57BL/6 mouse mesenteric arteriole over 12 min. Platelets were labeled with calcein. (WMV)

A new and straightforward procedure has been developed for the preparation of symmetrical and unsymmetrical 6-(N-alkyl(aryl)amino)- and 6-(N,N-bisalkyl(aryl)amino)-2,4-bis(alkyl(aryl)thio)pyrimidines. The two identical or different alkylthio groups were successfully introduced into the pyrimidine ring of 4-amino-6-hydroxy-2- mercaptopyrimidine via...

Local anesthetics (LAs) are necessary for the regional anesthesia, spinal anesthesia, and pain management. However, the application of LAs may cause neurotoxicity and result in postoperative neurological complications. Lithium is a mood stabilizer for the treatment of bipolar disorder and may exert neuroprotective effects. In this study, we evaluat...

A new and efficient procedure has been designed for the preparation of 6-alkylamino-2,4-dialkyl(aryl) thiopyrimidines. The first alkylthio group was introduced into the pyrimidine ring by S-alkylation. The introduction of the second one was successfully achieved using the diazotizationealkylthionation method to afford 2,4-dialkyl(aryl)thio-6-chloro...

Doppler centroid is an important parameter in synthetic aperture radar (SAR) image processing. Owing to measurement uncertainties, many clutter-lock algorithms are proposed to estimate the Doppler centroid. One of the factors that affect the estimation is `chirp coupling` [linear FM signal is used as transmitting signal in SAR, and this makes echoe...

Contents Male germ cells modified by foreign genes can be used to generate transgenic chicken. In this study, in vivo transfection of chicken testis with an EGFP‐LacZ dual reporter expression vector was performed. Large‐scale plasmid DNA preparation of the EGFP‐LacZ eukaryotic expression vector was carried out and efficient transfection of chicken...

Blazars from the first-three-months Fermi-AGN list were observed with the Urumqi 25-m radio telescope at 5 GHz in IDV (Intra-Day Variability) mode and inter-month observation mode. A significant correlation between the flux density at 5 GHz and the γ-ray intensity for the Fermi-LAT detected blazars is seen. There is a higher IDV detection rate in F...

Though antiplatelet drugs are proven beneficial to patients with coronary heart disease and stroke, more effective and safer antiplatelet drugs are still needed. In this study we report the antiplatelet effects and mechanism of BF0801, a novel adenine derivative. BF0801 dramatically inhibited platelet aggregation and ATP release induced by ADP, 2Me...

ChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 100 leading journals. To access a ChemInform Abstract of an article which was published elsewhere, please select a “Full Text” option. The original article is trackable via the “References” option.

Bupivacaine is one of the amide type local anesthetics and is widely used for epidural anesthesia and blockade of nerves. Bupivacaine administration locally could result in neuron injury showing transient neurologic symptoms. Dexamethasone is a synthetic glucocorticoid and may exert cytoprotective properties against damage induced by some stimuli....

The aim of this study was to construct a lentivirus vector that carries the human myxovirus-resistant A (MxA) anti-virus gene for efficiently infecting rooster spermatogonial stem cells (SSCs). A lentiviral vector carrying the enhanced green fluorescent protein (EGFP) and MxA fusion gene (EGFP-MxA) was constructed using TOPO technology in this stud...

A multiple-receiver Space-Surface Synthetic Aperture Radar (MRSS-BSAR) system is brought forward to improve azimuth resolution in SS-BSAR. The system is composed of one moving spaceborne illuminator and multiple static receivers. The receivers are arranged along the transmitter's moving direction with equal intervals. The profile in azimuth resolut...

This study aims to investigate the applicability of membrane distillation for ammonia removal from its aqueous solutions. Among the different recognized membrane distillation configurations, vacuum membrane distillation is applied in the present work for ammonia removal. The effects of different operating parameters on ammonia removal from aqueous...

Membrane distillation (MD) is an emerging technology for separations that are traditionally accomplished by conventional separation processes such as distillation or reverse osmosis. Since its appearance in the late of the 1960s and its development in the early of 1980s with the growth of membrane engineering, MD claims to be a cost effective separ...

This work presents a low complexity bootstrapping filter for target direction-of-arrival tracking in array signal processing, and derives a Monte Carlo maximum likelihood method. Similar to bootstrapping, our new algorithm bypasses the resampling step and directly generates the maximum-likelihood estimates. This simpler target tracking method is co...

Human platelets express two P2Y receptors: G(q)-coupled P2Y(1), and G(i)-coupled P2Y(12). Both P2Y(1) and P2Y(12) are ADP receptors on human platelets and are essential for ADP-induced platelet aggregation that plays pivotal roles in thrombosis and hemostasis. Numerous constitutively active G protein-coupled receptors have been described in natural...

Based on the two-step algorithm and range migration algorithm the deramp range migration algorithm for the high-resolution satellite-borne spotlight synthetic aperture radar SAR is presented The algorithm combines the advantages of SPECAN algorithm and range migration algorithm The first step of the proposed algorithm implements a linear and space-...

Human platelets express two P2Y receptors: Gq-coupled P2Y1 and Gi-coupled P2Y12. Both P2Y1 and P2Y12 are ADP receptors on human platelets and are essential for ADP-induced platelet aggregation that plays pivotal roles in thrombosis and hemostasis. Numerous constitutively active G protein-coupled receptors have been described in natural or recombina...

Polycrystalline samples La1.85Sr0.15Cu1−x Znx O4−δ (0≤ x≤ 0.3), La1.85Sr0.15Cu1−x Nix O4−δ (0≤ x≤ 0.3), and La1.85Sr0.15Cu1−x Mgx O4−δ (0≤ x≤ 0.3) were synthesized by the solid-state reaction method. The crystal structure and phonon vibration were investigated by means of X-ray diffraction (XRD) and infrared spectrum. Zn, Ni, and Mg doping results...

The P2Y(1) ADP receptor activates G(q) and causes increases in intracellular Ca(2+) concentration through stimulation of PLC. In this study, we investigated the role of the amino acid residues in the COOH terminus of the human P2Y(1) receptor in G(q) activation. Stimulation of Chinese hamster ovary (CHO-K1) cells stably expressing the wild-type hum...

The aim of this study was to analyze the distribution of plasminogen activator inhibitor-1 (PAI-1) promoter 4G/5G polymorphism in healthy Chinese and compare the transcription activity of the two PAI-1 promoters using luciferase reporter vector pGL(3). PAI-1 promoter 4G/5G polymorphism was analyzed in 77 healthy Chinese using allele-specific polyme...

ADP-induced platelet aggregation plays an important role in hemostasis and thrombosis. Human platelets express two ADP receptors: the P2Y1 and P2Y12 receptors. The Gq-activating P2Y1 receptor plays an important role in ADP-induced platelet shape change, aggregation, and thromboxane A2 generation. In this study, we investigated the role of the carbo...

The role of the G(i)-coupled platelet P2Y(12) receptor in platelet function has been well established. However, the functional effector or effectors contributing directly to alphaIIbbeta3 activation in human platelets has not been delineated. As the P2Y(12) receptor has been shown to activate G protein-gated, inwardly rectifying potassium (GIRK) ch...

The thermal decomposition of syngenite, K2Ca(SO4)2·H2O, formed during the treatment of liquid manure has been studied by thermal gravimetric analysis, differential scanning calorimetry, high temperature X-ray diffraction (XRD) and infrared emission spectroscopy (IES). Gypsum was found as a minor impurity resulting in a minor weight loss due to dehy...

Nucleotides are released during vascular injury from activated platelets and broken cells, which could stimulate human neutrophils. In this study, we characterized the P2Y receptors and investigated the functional effects of extracellular nucleotides on human neutrophils. Pharmacological characterization using selective agonists and pertussis toxin...

A combination of differential scanning calorimetry (DSC) and high-resolution differential thermogravimetric analysis (DTGA) coupled to a gas evolution mass spectrometer has been used to study the thermal properties of a chromium based series of Cu/Zn hydrotalcites of formulae CuxZn6−xCr2(OH)16(CO3)·4H2O where x varied from 6 to 0.The spacing for th...

The contamination of soils with heavy metals such as As, Cr and Cu is of great importance; the remediation of such soils even more so. Arsenic compounds are prevalent in soils either through leaching of mine tailings, the use of Cu/Cr/As as a wood preservative or through the use of arsenic in cattle dips. The arsenic compounds in soils and leachate...

Human platelets express 2 G protein-coupled nucleotide receptors: the platelet adenosine diphosphate (ADP) receptor coupled to stimulation of phospholipase C (P2Y(1)) via heterotrimeric guanosine 5-triphosphate (GTP)-binding protein G(q), and the platelet ADP receptor coupled to inhibition of adenylyl cyclase (P2Y(12)) via heterotrimeric GTP-bindin...

A combination of differential scanning calorimetry (DSC) and high resolution DTG coupled to a gas evolution mass spectrometer has been used to study the thermal properties of a series of Cu/Zn hydrotalcites of formulae CuxZn6−xAl2(OH)16(CO3)·4H2O where x varied from 6 to 0. The effect of increased Zn composition results in the increase of the endot...

Differential scanning calorimetry shows two endotherms at 75 and 225 degrees Celsius for synthetic goethite. The latter endotherm is strongly asymmetric on the low temperature side. The endotherms were attributed to the loss of water and the dehydroxylation of the goethite. The temperature of the endotherms and the enthalpy of the phase change were...

A series of sepiolites, palygorskites and “Rocky Mountain Leather” (RML) clay minerals have been analysed by controlled rate thermal analysis and differential scanning calorimetry (DSC). Eight weight loss steps are observed and are structure and composition dependent. Three dehydration steps and five dehydroxylation steps are observed. The mass spe...

A combination of DSC and high resolution DTG coupled to a gas evolution mass spectrometer has been used to study the thermal properties of a series of Mg/Zn hydrotalcites of formulae MgxZn6-xAl2(OH)16(CO3) ·4H2O where x varied from 6 to 0. The effect of increased Zn composition results in the decrease of the endotherms and mass loss steps to lower...

Infrared and Raman spectroscopy have been used to characterise synthetic hydrotalcites of formula Cu(x)Zn(6 - x)Al2(OH)16(CO3) x 4H2O. The spectra have been used to assess the molecular assembly of the cations in the hydrotalcite structure. The spectra may be conveniently subdivided into spectral features based (a) upon the carbonate anion (b) the...

Platelet P2 receptors--P2Y1, P2Y12, and P2X1--constitute the means by which adenine nucleotides can activate platelets. Coactivation of the Galphaq-coupled P2Y1 and Galphai2-coupled P2Y12 receptors is necessary for ADP-mediated platelet activation, which forms the basis of using P2 antagonists as antithrombotic drugs. P2Y1 receptor antagonists inhi...

The pore structure formation in bentonite, pillared with a mixed sol of silicon and titanium hydroxides and treated subsequently with quaternary ammonium surfactants, is investigated. The surfactant micelles act as a template, similar to their role in MCM41 synthesis. Because both the surfactant micelles and the sol particles are positively charged...

The growth and persistence of solid tumors and their metastasis are angiogenesis-dependent. Vasostatin, the N-terminal domain of calreticulin inclusive of amino acids 1-180, is a potent angiogenesis inhibitor. To investigate whether intramuscular administration of vasostatin gene has the antitumor activity in mouse tumor models, we constructed a pl...

Azurite and malachite have been extensively used as pigments in ancient and medieval manuscripts, glasses and glazes. The thermal stability of naturally occurring azurite and malachite was determined using a combination of controlled rate thermal analysis (CRTA) combined with mass spectrometry and infrared emission spectroscopy. Both azurite and ma...

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The thermal decomposition of a series of nontronites and a ferruginous smectite has been studied using a combination of high-resolution thermogravimetric analysis (TGA) with a controlled heating rate and the mass spectrometry (MS) of evolved water vapour. The temperature–mass spectrum curve follows the differential TGA (DTGA) curve with precision,...

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The oscillatory shear index (OSI) was developed based on the hypothesis that intimal hyperplasia was correlated with oscillatory shear stresses. However, the validity of the OSI was in question since the correlation between intimal thickness and the OSI at the side walls of the sinus in the Y-shaped model of the average human carotid bifurcation (Y...

XK469 (2-[4-(7-chloro-2-quinoxalinyloxy) phenoxy]propionic acid), a synthetic quinoxaline phenoxypropionic acid derivative, has broad activity against murine tumors and is entering Phase I clinical development as a topoisomerase IIbeta inhibitor. This study investigated the underlying molecular mechanism of XK469's effects on the cell cycle. Growth...

Phase-resolved optical Doppler tomography (ODT) has very high sensitivity while maintaining a fast axial scanning rate, making it possible to reconstruct blood flow in three dimensions. Here we demonstrate an ODT system that employed novel signal-processing techniques to remove aliasing effects and artifacts caused by lateral scanning and target mo...

Metal oxide pillared clay (PILC) possesses several interesting properties, such as large surface area, high pore volume and tunable pore size (from micropore to mesopore), high thermal stability, strong surface acidity and catalytic active substrates/metal oxide pillars. These unique characteristics make PILC an attractive material in catalytic rea...