Zhisheng Dang

Chinese Center For Disease Control And Prevention | CDC · National Institute of Parasitic Diseases

Echinococcus multilocularis is a small parasite that causes alveolar echinococcosis. It primarily induces liver disorder, such as liver fibrosis and even liver cancer, which severely endangers human lives. This study aims to explore the efficacy of Echinococcus multilocularis soluble antigen in preventing and alleviating alveolar echinococcosis-ind...

Background Cystic echinococcosis (CE), a condition caused by the larval stage of the dog tapeworm Echinococcus granulosus sensu stricto, is a globally distributed zoonotic disease. Current treatment options for CE are limited, and an effective and safe anti-echinococcal drug is urgently required. Methods Drug repurposing strategy was employed to i...

Recently, we introduced an epoxy group to mebendazole by a reaction with epichlorohydrin and obtained two isoforms, mebendazole C1 (M-C1) and mebendazole C2 (M-C2). The in vitro effects of mebendazole derivatives at different concentrations on Echinococcus multilocularis protoscoleces and metacestodes as well as cytotoxicity in rat hepatoma (RH) ce...

According to conservative estimates, >230 million people are infected with schistosomiasis,which becomes one of the most common parasitic diseases. This study focuses on investigating in vivo and in vitro effects of mmu-miR-92a-2-5p in Schistosoma japonicum-induced liver fibrosis by targeting TLR2. Through bioinformatic analysis, the overexpression...

Objectives In our research, we aimed to investigate the roles of CC‐chemokine receptor 7 (CCR7) and relevant signaling pathways in Leishmania major‐infected human dendritic cells (DCs). Methods Differentially expressed genes (DEGs) in L. major‐infected human DCs were selected out and visualized using R program. Kyoto Encyclopedia of Genes and Geno...

Benzimidazoles, including albendazole and mebendazole, are the major drugs for clinical chemotherapy of echinococcosis. They mainly exert parasitostatic effects depending on high dosages for long-term. Previous studies have identified carbazole aminoalcohols as novel anti-CE (cystic echinococcosis) agents. However, it is still to be confirmed wheth...

Background: Interventions are currently being used against 'infectious diseases of poverty', which remain highly debilitating and deadly in most endemic countries, especially malaria, schistosomiasis, echinococcosis and African sleeping sickness. However, major limitations of current 'traditional' methods for diagnosis are neither simple nor conve...

In order to understand the epidemiological status of alveolar and cystic echinococcosis in intermediate and definitive hosts in Qinghai Province, China, during the period 2007–2011, we investigated the infection in humans and animals, including yaks, Tibetan sheep, Tibetan dogs, and wild foxes distributed in different counties around the province....

Echinococcus multilocularis is a tiny devastating worm that causes alveolar echinococcosis in humans. This disease mainly occurs in the liver but rarely in other organs. We report the subcutaneous encystment of E. multilocularis metacestodes in experimentally infected mice. Subcutaneous cysts had remarkably fewer protoscoleces (2.05 ± 1.47, n = 20)...

Alveolar echinococcosis (AE) is a worldwide zoonosis caused by E.multilocularis. Humans become infected through oral ingestion of the eggs. Host of E. multilocularis produces immune responses that help to either reject and/or limit the growth of this parasite, and in response the parasite produces molecules against this immune attack. This study id...

Objective: The study aimed to explore the regulation of heat shock protein 47 (HSP47) on expressions of receptors associated with hepatic stellate cell (HSC) in liver fibrosis mouse models induced by Schistosoma japonicum (S. japonicum). Mouse fibroblasts (NIH/3T3) were transfected with HSP47 shRNA plasmid by lipofectamine transfection, and experi...

Background: Acute diarrhea is one of the major public health problems worldwide. Most of studies on acute diarrhea have been made on infants aged below 5 years and few efforts have been made to identify the etiological agents of acute diarrhea in people over five, especially in China. Methods: 271 diarrhea cases and 149 healthy controls over 5 y...

Background Acute diarrhea is a global health problem, resulting in high morbidity and mortality in children. It has been suggested that enteric pathogen co-infections play an important role in gastroenteritis, but most research efforts have only focused on a small range of species belonging to a few pathogen groups. This study aimed to assess the i...

Author Humans become infected with E. multilocularis through oral ingestion of the eggs. Untreated alveolar echinococcosis has a fatality rate of >90% in humans, causing great public health concern in the northern hemisphere. The genome databases of E. multilocularis have been recently published, but still there is no available data about transcri...

BLASTX results of putative Em-TSP3 isoforms from different samples. (XLSX)

Nucleotide sequences of putative Em-TSP3. (XLSX)

Significant different expressed genes in Non-activated oncospheres, Activted oncospheres and early stage of Metacestodes from Echinococcus multilocularis (P<0.01, FDR<0.05). (XLSX)

IGV view of putative Em-TSP3 isoforms in pathogen_EMU_scaffold_007780 of E. multilocularis genome versions 3. The five tracks show the mapped result of EmuJ_001077300 (gene start: 13002559, gene end: 13003475) and EmuJ_001077400 (gene start: 13008382, gene end: 13009298) which all most reads can mapped to the two putative Em-TSP3 isoforms. At the l...

Transcript data of dignostic antigen gp50 family in any of the sampled life cycle stage in this study and previous report [23]. (XLSX)

The objective of this article was to investigate the morphological and molecular characterization of Oestromyia leporina (Pallas, 1778) from wild plateau pikas (O. curzoniae) in Qinghai province, China. The third-stage larvae of O. leporina were examined by scanning electron microscopy revealing morphology characteristics of the spines on the cepha...

The prevalence of Hypoderma spp. in yaks grazed in the east of Qinghai province was investigated in 2008. In this area, the prevalence in young yaks (1- to 3-year-old) was very high at 82.2-98.7%, whilst in adult yaks (4-year-old and older), the prevalence was 42.4-50.6%. The seasonal development and migration pattern of Hypoderma larvae in yak bod...

Currently, the most efficient and widely used method for tick control is the application of acaricides, especially deltamethrin and alpha-cypermethrin, two pyrethroids with neurotoxic action. In this study, the in vitro efficacy of deltamethrin and alpha-cypermethrin was assessed on engorged female Haemaphysalis qinghaiensis ticks. An in vitro bioa...

Schematic representation of mucosal anti-rEm-TSP3 specific IgA production against Echinococcus oncospheres enhanced by rEm-TSP3-FBP. FBP of Em-TSP3-FBP (T–F) facilities binding of fusion protein to fibronectin (F) of microfold cells (M), a subepithelial dome rich in dendritic cells (DC), B cells (B) and plasma cells (P). After Em-TSP3-FBP is transp...

List of Genbank accession numbers for the genes referred to in the text. (DOC)

BACKGROUND: Studies have shown that a bacterial fibronectin attachment protein (FAP) is able to stimulate strong systemic and mucosal antibody responses when it is used alone or co-administrated with other antigens (Ags). Thus, it has been suggested to be a promising adjuvant candidate for the development of efficient vaccines. However, the co-admi...

Background We have previously evaluated the vaccine efficacies of seven tetraspanins of Echinococcus multilocularis (Em-TSP1–7) against alveolar echinococcosis (AE) by subcutaneous (s.c.) administration with Freund's adjuvant. Over 85% of liver cyst lesion number reductions (CLNR) were achieved by recombinant Em-TSP1 (rEm-TSP1) and -TSP3 (rEm-TSP3)...

Cyst lesions formed by E. multilocularis larvae in BALB/c mice liver. Immunized mice were anesthetized and challenged orally with 200 E. multilocularis eggs. One month post-infection, all mice were sacrificed and the livers were collected and cut into slices to count the number of cyst lesions. Cysts are marked in blue circles. (TIF)

Classical morphology of an egg from the feces of an experimentally-infected dog. E = embryophore; O = oncosphere; H = hook. (TIF)

List of Genbank accession numbers for the genes referred to in the text. (DOC)

We have previously evaluated the vaccine efficacies of seven tetraspanins of Echinococcus multilocularis (Em-TSP1-7) against alveolar echinococcosis (AE) by subcutaneous (s.c.) administration with Freund's adjuvant. Over 85% of liver cyst lesion number reductions (CLNR) were achieved by recombinant Em-TSP1 (rEm-TSP1) and -TSP3 (rEm-TSP3). However,...

Alveolar echinococcosis (AE) is a severe hepatic disorder caused by larval infection by the fox tapeworm Echinococcus multilocularis. The course of parasitic development and host reactions are known to vary significantly among host species, and even among different inbred strains of mice. As reported previously, after oral administration of parasit...

Echinococcus multilocularis causes an important zoonotic cestode disease. The metacestode stage proliferates in the liver of intermediate hosts including human and rodents and forms multiple cysts. Recently, members of a transmembrane protein tetraspanin (TSP) family have been used as vaccines against schistosomosis, or as diagnostic antigens for c...

One tetraspanin, designated as E24, was cloned from a full-length enriched vector-capping cDNA library of Echinococcus multilocularis metacestode. The amino acid sequence and phylogenetic analysis suggested that E24 is a T24-like protein. The crucial, functional large extracellular loop (LEL) domain of E24 was expressed and characterized using a po...

The experimental transmission of a recently designated Theileria uilenbergi pathogenic for sheep and goats in Northern China is described. Haemaphysalis qinghaiensis nymphs and adults developed from larvae and nymphs engorged on sheep infected with T. uilenbergi were able to respectively transmit the latter to sheep. However, when H. longicornis ti...

A reverse line blot (RLB) assay was developed for detection and specific identification of the different ovine Theileria and Babesia parasites. In a polymerase chain reaction (PCR), the hypervariable region 4 (V4 region) of the 18S ribosomal DNA gene was amplified with a set of general primers specific for members of the genera Theileria and Babesi...

Recently, Theileria sp. (China) has been designated as T. luwenshuni[formerly Theileria sp. (China 1)] and T. uilenbergi[formerly Theileria sp. (China 2)]. A cDNA library of T. uilenbergi merozoites was constructed and subjected to random sequencing. Among the obtained sequences were three highly identical cDNA clones, indicating a gene family. Bio...

Positive clone Hq05 of Haemaphysalis qinghaiensis (named following those cDNA clones cloned from the tick before) was obtained by differentially screening of a cDNA library of the tick with rabbit anti-tick salivary gland serum and rabbit anti-tick saliva serum. Hq05 contains an open reading frame (ORF) of 540bp that codes for 179amino acid residue...

The loop-mediated isothermal amplification (LAMP) reaction is a method that amplifies with high sensitivity, efficiency, and rapidity, deoxyribonucleic acid (DNA) under isothermal condition in simple incubators. Two primer sets for the LAMP method were designed using the nucleotide sequences of 18S rRNA gene of Babesia sp. BQ1 (Lintan) and Babesia...

The application of anti-tick vaccine has been shown to be the most promising alternative strategy compared to the current use of acaricides that suffer from a number of serious limitations. The success of this method is dependent upon identification and cloning of potential tick vaccine antigens. Previously, we have cloned 21 positive clones (named...

Babesia ovata and Babesia major are two newly identified large Babesia species infective to cattle in China. There is a demand for specific tools for discrimination between the two species due to the confusion of their classification based on traditionally classification methods, such as tick vector, morphology, and pathogenicity. In this study, th...

The experimental transmission of a recently identified new Theileria (China 1) species pathogenic for sheep and goats in northern China is described. Haemaphysalis qinghaiensis nymphs and adults developed from larvae and nymph engorged on sheep infected with Theileria sp. (China 1) were able to respectively transmit the Theileria sp. to splenectomi...

A fatal disease of sheep and goats in the northern part of China has been reported to be due to Babesia ovis. However, some characteristics of the causative agent in recent reports are not in accordance with the original attributes ascribed to this parasite. Therefore, the 18S small subunit ribosomal RNA (18S rRNA) genes of a number of Babesia isol...

A large-scale serological surveillance was conducted for hypodermosis in the north of China. A total of 4175 sera samples of yaks and cattle were collected from five provinces in north China that included Xinjiang, Inner Mongolia, Heilongjiang, Jiling and Gansu provinces, between 2001 and 2002, and were examined for anti-hypoderma antibody by ELISA...

The ribosomal small-subunit RNA gene sequences of six Chinese Babesia stocks infective to cattle, including a Babesia bigemina isolate, a B. bovis isolate, two B. ovata isolates, a Babesia sp. Wenchuan isolate and a B. major isolate, were compared and analyzed. The target DNA segment was amplified by polymerase chain reaction and the product ligate...