
Zhifeng YouWenzhou Institute of Biomaterials and Engineering
Zhifeng You
Doctor of Engineering
About
23
Publications
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740
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Introduction
Education
August 2015 - June 2020
Publications
Publications (23)
Neural regeneration after spinal cord injury (SCI) closely relates to the microvascular endothelial cell (MEC)-mediated neurovascular unit formation. However, the effects of central nerve system-derived MECs on neovascularization and neurogenesis, and potential signaling involved therein, are unclear. Here, we established a primary spinal cord-deri...
Controllable drug delivery systems (DDS) can overcome the disadvantages of conventional drug administration processes, such as high dosages or repeated administration. Herein, a smart DDS collagen hydrogel is deployed for spinal cord injury (SCI) repair based on modular designing of “egg” nanoparticles (NPs) that ingeniously accomplish controlled d...
High levels of reactive oxygen species (ROS) and inflammation create a complicated extrinsic neural environment that dominates the initial post‐injury period after spinal cord injury (SCI). The compensatory pathways between ROS and inflammation limited the efficacy of modulating the above single treatment regimen after SCI. Here, novel “nanoflower”...
Transplantation of allogeneic adult spinal cord tissues (aSCTs) to replace the injured spinal cord, serves as a promising strategy in complete spinal cord injury (SCI) repair. However, in addition to allograft immune rejection, damage-associated molecular pattern (DAMP)-mediated inflammatory microenvironments greatly impair the survival and functio...
Spinal Cord Injury Repair
Comprehensive approaches based on biomaterials provide topographical, biophysical, and biochemical cues to regulate neural regeneration and microenvironment to promote spinal cord injury (SCI) repair. In article number 2110628, Yannan Zhao, Jianwu Dai, and co‐workers review the current status, recent advances, challenges,...
Spinal cord injury (SCI) often leads to the loss of motor and sensory functions and is a major challenge in neurological clinical practice. Understanding the pathophysiological changes and the inhibitory microenvironment is crucial to enable the identification of potential mechanisms for functional restoration and to provide guidance for the develo...
He Shen Bai Xu Chao Yang- [...]
Jianwu Dai
Spinal cord injury (SCI) creates an inflammatory microenvironment characterized by damage-associated molecular patterns (DAMPs) and immune cell activation that exacerbate secondary damage and impair neurological recovery. Here we develop an immunoregulatory hydrogel scaffold for treating SCI that scavenges DAMPs and slowly releases the anti-inflamm...
Keloids are fibrotic lesions that grow unceasingly and invasively and are driven by local mechanical stimuli. Unlike other fibrotic diseases and normal wound healing, keloids exhibit little transformation of dermal fibroblasts into α‐SMA⁺ myofibroblasts. This study showed that asporin is the most strongly expressed gene in keloids and its gene‐onto...
In article number 2000531 by Chenyu Huang, Yanan Du and co‐workers, a microtissue assembly in 3D‐printed‐template‐scaffold (3D‐MAPS) is developed to rapidly fabricate centimeter‐sized tissue constructs by bottom‐up assembly of large‐scale manufactured microtissues within 3D‐printed hollow polymeric templates with pre‐defined architectures.
Tissue engineering using traditional size fixed scaffolds and injectable biomaterials are faced with many limitations due to the difficulties of producing macroscopic functional tissues. In this study, 3D functional tissue constructs were developed by inducing self-assembly of microniches, which were cell-laden gelatin microcryogels. During self-as...
Engineering large and functional tissue constructs with complex structures (e.g., external ear or nose) for reparation and reconstruction of tissue defects remains one of the major challenges in regenerative medicine, which demands abundant cell sources, advanced biofabrication schemes, and satisfactory integration with the host for long‐term effic...
Background:
In knee osteoarthritis (OA), there is more pronounced cartilage damage in the medial compartment ('lesion zone') than the lateral compartment ('remote zone'). This study fills a gap in the literature by conducting a systematic comparison of cartilage and chondrocyte characteristics from these two zones. It also investigates whether cho...
Significance
In the context of myofibroblast–fibroblast interaction during fibrosis expansion, the mechanistic insights of intercellular mechanical communication have been poorly evidenced. We systematically investigated the myofibroblast–fibroblast cross talk via the fibrous matrix, which we termed “paratensile signaling” in the context of fibrosi...
Cutaneous scars are believed to be due to aberrant wound healing that alters the levels of collagens in the dermis. Definite diagnoses of scar types that clearly distinguish them from other scar types will facilitate the selection of the most appropriate therapies for individual scars and patients. To this end, cutaneous scars are classified as nor...
Tissue stiffness, shear stress, and interstitial pressure constitute major factors of the liver mechanical microenvironment that play a key regulatory role in controlling cell behavior in the liver and progression of liver diseases. In this review, we focus on the characteristics of the liver mechanical microenvironment and summarize cellular respo...
The aetiology of keloids is becoming clearer, but many questions remain, including about the most optimal treatment. Current therapies include surgical excision, radiotherapy, and various pharmaceutical drugs. However, none of these drugs are keloid‐specific. Moreover, all current interventions are associated with high recurrence rates. Here, we re...
Hypertrophic scars (HSs) and keloids, as the commonly seen pathological scars, are characteristic of rampant proliferation of fibroblasts, accumulation of collagens in the ECM, and altered regulation of growth factors/cytokines. Though their etiology still remains unclear and the postsurgical recurrence keeps a high profile, great efforts have been...
The role of pathological angiogenesis on liver fibrogenesis is still unknown. Here, we developed fibrotic microniches (FμNs) that recapitulate the interaction of liver sinusoid endothelial cells (LSECs) and hepatic stellate cells (HSCs). We investigated how the mechanical properties of their substrates affect the formation of capillary-like structu...
To upgrade traditional 2D cell culture to 3D cell culture, we have integrated microfabrication with cryogelation technology to produce macroporous microscale cryogels (microcryogels), which can be loaded with a variety of cell types to form 3D microtissues. Herein, we present the protocol to fabricate versatile 3D microtissues and their application...
Hypertrophic scars/keloids are pathogenic scars that are characterized by collagen and fibroblast accumulation. The endothelia in the lesions are mechanosensitive and participate actively in the pathogenesis of these scars. The present review summarizes how endothelium responds to mechanical stimuli such as shear, stretch, and hydrostatic pressure....
Targeted cell delivery to lesion sites via minimally invasive approach remains an unmet need in regenerative medicine to endow satisfactory therapeutic efficacy and minimized side-effects. Here, we rationally designed a pathology-targeted cell delivery strategy leveraging injectable micro-scaffolds as cell-loading capsule and endogenous tissue tran...
Keloids are fibroproliferative skin disorders characterised clinically by continuous horizontal progression and post-surgical recurrence and histologically by the accumulation of collagen and fibroblast ingredients. Till now, their aetiology remains clear, which may cover genetic, environmental and metabolic factors. Evidence in the involvement of...