Zhenying Peng

Zhenying Peng
Shandong Academy of Agricultural Sciences | SAAS · Biotechnology Research Center

About

56
Publications
3,818
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586
Citations
Citations since 2017
28 Research Items
358 Citations
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2017201820192020202120222023020406080
2017201820192020202120222023020406080
2017201820192020202120222023020406080

Publications

Publications (56)
Article
Full-text available
Triacylglycerols is the major storage lipid in most crop seeds. As the key enzyme catalyzing the final step of triacylglycerols biosynthesis, the activity of diacylglycerol acyltransferaseis directly related to oil content. It has been shown that certain amino acids are very important for enzyme activity, one amino acid variation will greatly chang...
Article
Full-text available
Background Small auxin-upregulated RNAs ( SAURs ) gene family plays important roles in plant growth, development, and stress responses. However, the function of few SAUR genes is known in the peanut ( Arachis hypogaea L.), one of the world’s major food legume crops. This study aimed to perform a comprehensive identification of the SAUR gene family...
Article
Full-text available
The NAC transcription factor (TF) is one of the most significant TFs in plants and is widely involved in plant growth, development, and responses to biotic and abiotic stresses. To date, there are no systematic studies on the NAC family in peanuts. Herein, 132 AhNACs were identified from the genome of cultivated peanut, and they were classified int...
Article
Full-text available
Fatty acyl-acyl thioesterases (FATs), which hydrolyze the thioester bond linking acyl chains to an acyl carrier protein, thereby terminating their elongation, contribute significantly to the fatty acid (FA) content and composition of seed storage lipids. The peanut (Arachis hypogaea L.) genome was found to harbor 21 FAT (AhFAT) genes, distributed o...
Article
Full-text available
Background: Long noncoding RNAs (lncRNAs), which are typically > 200 nt in length, are involved in numerous biological processes. Studies on lncRNAs in the cultivated peanut (Arachis hypogaea L.) largely remain unknown. Results: A genome-wide scan of the peanut (Arachis hypogaea L.) transcriptome identified 1442 lncRNAs, which were encoded by lo...
Article
Full-text available
The synthesis of α-linolenic acid (ALA) requires the activity of ω-3 fatty acid desaturases (ω-3 FADs). The quality of peanut oil would be much improved if the content of ALA could be increased. A scan of the peanut genome revealed that it harbored 36 FAD genes, mapping to 16 of the species’ 20 chromosomes. A phylogenetic analysis concluded that th...
Preprint
Full-text available
Background Long noncoding RNAs (lncRNAs), which are typically >200 nt in length, are involved in numerous biological processes. Studies on lncRNAs in the cultivated peanut (Arachis hypogaea L.) largely remain unknown. Results A genome-wide scan of the peanut (Arachis hypogaea L.) transcriptome identified 1,442 lncRNAs, which were encoded by loci di...
Article
Full-text available
Background: Posttranslational modification of proteins by small ubiquitin like modifier (SUMO) proteins play an important role during the developmental process and in response to abiotic stresses in plants. However, little is known about SUMOylation in peanut (Arachis hypogaea L.), one of the world's major food legume crops. In this study, we char...
Article
Full-text available
Background: Alternative splicing (AS) represents a mechanism widely used by eukaryotes for the post-transcriptional regulation of genes. The detailed exploration of AS in peanut has not been documented. Results: The strand-specific RNA-Seq technique was exploited to characterize the distribution of AS in the four samples of peanut (FH1-seed1, FH...
Article
Full-text available
Palmitoleic acid (C16:1) and stearic acid (C18:0) are precursors of polyunsaturated fatty acids, which are the focus of intensive global research due to their nutritional value, medicinal applications, and potential use as biofuel. Acyl-acyl carrier protein (ACP) thioesterases are intraplastidial enzymes that determine the types and amounts of fatt...
Article
Full-text available
Diacylglycerol acyltransferase (DGAT) catalyzes the final step in triacylglycerol (TAG) biosynthesis via the acyl-CoA-dependent acylation of diacylglycerol. This reaction is a major control point in the Kennedy pathway for biosynthesis of TAG, which is the most important form of stored metabolic energy in most oil-producing plants. In this study, A...
Data
Nucleotide sequence comparison of AhDGAT2a-h.
Data
A diagram of the FA metabolism in plant. (1) acetyl-CoA carboxylase (ACC); (2) malonyl-CoA:ACP malonyltransferase (MT); (3) ketoacyl-ACP (KSIII synthase); (4) ketoacyl-ACP synthase (KSI); (5) ketoacyl-ACP reductase (KR); (6) hydroxyacyl-ACP dehydrase (HAD); (7) enoyl-ACP reductase (ER); (8) ketoacyl-ACP synthase (KSII); (9) stearoyl-ACP desaturase...
Data
Quantification of Nile Red fluorescence intensity of yeast strains expressing variants of peanut DGAT2a. The values shown are the averages of four measurements from each yeast strain; error bars represent the standard error of measurement. ∗Compared to pESC-URA, student’s unpaired t-test, P < 0.05; ∗∗Compared to pESC-URA, P < 0.01; #Compared to AhD...
Article
Triacylglycerols (TAGs) are the most important energy storage form in oilseed crops. Diacylglycerol acyltransferase (DGAT) catalyzes the rate-limiting step of the Kennedy pathway of TAG biosynthesis. To date, little is known about the regulation of DGAT activity in peanut (Arachis hypogaea), an agronomically important oilseed crop that is cultivate...
Article
Full-text available
The effects of various cultivation modes of I. zhanjiangensis in different photobioreactors are studied to achieve sustainable high biomass and lipid productivity. The kinetic culture characteristics and lipid accumulation of the microalgae I. zhanjiangensis are evaluated in column and flat plate photobioreactor in batch and semi-continuous mode. T...
Article
Full-text available
MYB transcription factors (TFs) in plants play important roles in abiotic stress tolerance. Here, we report that an MYB-related TF gene, named FvMYB1, was isolated from the salt-tolerant tree species Fraxinus velutina Torr. The real-time PCR analysis showed that the FvMYB1 transcript increased remarkably in response to salt treatment. Subsequently,...
Article
Full-text available
The demand for INSULIN is increasing rapidly along with the increased number of diabetic patients. Using the CRE/loxP system, we developed a selective marker-free system without crossing to produce PROINSULIN in transgenic plant. In frame of this approach, the induced promoter pRD29A was isolated from Arabidopsis. The CRE recombinase gene was place...
Article
Full-text available
We previously bred a salt tolerant wheat cv. SR3 with bread wheat cv. JN177 as the parent via asymmetric somatic hybridization, and found that the tolerance is partially attributed to the superior photosynthesis capacity. Here, we compared the proteomes of two cultivars to unravel the basis of superior photosynthesis capacity. In the maps of two di...
Article
Full-text available
Genetic modification is useful for improving the nutritional qualities of cyanobacteria. To increase the total unsaturated fatty acid content, along with the ratio of ω-3/ω-6 fatty acids, genetic engineering can be used to modify fatty acid metabolism. Synechococcus sp. PCC7002, a fast-growing cyanobacterium, does not contain a Δ6 desaturase gene a...
Article
Diacylglycerol acyltransferase (DGAT) is a rate-limiting enzyme in triacylglycerol (TAG) biosynthesis pathway. In this study, GenomeWalking method was used for cloning the promoter sequence of AhDGAT2a gene from Luhua 14, and finally a 1200 bp fragment flanking 5'-upstream of AhDGAT2a was obtained and named as pAhDGAT2a. The crucial regulatory elem...
Article
Full-text available
The increased incidence of diabetes, coupled with the introduction of alternative insulin delivery methods that rely on higher doses, is expected to result in a substantial escalation in the future demand for affordable insulin. Plant-based systems offer a safe and economical method for producing pharmaceutical proteins. We used peanut (Arachis hyp...
Article
Full-text available
The relationship between salt bridges and stability/enzymatic activity is unclear. We studied this relationship by systematic alanine-scanning mutation analysis using the typical M4 family metalloprotease Pseudomonas aeruginosa elastase (PAE, also known as pseudolysin) as a model. Structural analysis revealed seven salt bridges in the PAE structure...
Data
Full-text available
Acyl-acyl carrier protein thioesterases are intraplastidial enzymes that determine the types and amounts of fatty acids found in plants and then release them into the cytosol to be incorporated into glycerolipids. To better understand the role of the FatB class of acyl-acyl carrier protein thioesterase, a 1.2 kb cDNA fragment of AhFatB1 was amplifi...
Article
Full-text available
Polyunsaturated fatty acids (PUFAs), which contain two or more double bonds in their backbone, are the focus of intensive global research, because of their nutritional value, medicinal applications, and potential use as biofuel. However, the ability to produce these economically important compounds is limited, because it is both expensive and techn...
Article
Full-text available
Diacylglycerol acyltransferase (DGAT) is the rate-limiting enzyme in triacylglycerol biosynthesis in eukaryotic organisms. Triacylglycerols are important energy-storage oils in plants such as peanuts, soybeans and rape. In this study, Arachis hypogaea type 2 DGAT (AhDGAT2) genes were cloned from the peanut cultivar 'Luhua 14' using a homologous gen...
Data
Comparison of the nucleotide sequences of AhDGAT2a and AhDGAT2b. The 14 nucleotide differences are shaded in gray. The red underlines show the initiation and termination codons. (TIF)
Data
Original data and data analysis. (XLS)
Article
V-myb avian myeloblastosis viral oncogene homolog (MYB) transcription factors play important roles in the processes of plant growth, development, and stress responses. In this study, a full-length cDNA sequence of a MYB gene, designated FvMYB1, was isolated from Arizona ash (Fraxinus velutina Torr.) for the first time. Sequence analysis showed that...
Article
Objectives: The study was designed to provide a basis for understanding the codon usage bias of the Pyramimonas cordata isolate CS-140 delta 6 fatty acid elongase and selecting suitble expression systems for heterologous protein production. Methods: CodonW and CUSP programs were applied to calculate the effective number of codon (ENc) value, nucleo...
Article
Full-text available
In this study, a full-length cDNA of the acyl-ACP thioesterase, AhFatA, was cloned from developing seeds of Arachis hypogaea L. by 3'-RACE. Sequence analysis showed that the open reading frame encodes a peptide of 372 amino acids and showed 50-70% identity with FatA from other plants. Real-time quantitative PCR analysis revealed that AhFatA was exp...
Article
Full-text available
In this study, a full-length cDNA of the acyl-ACP thioesterase, AhFatA, was cloned from developing seeds of Arachis hypogaea L. by 3'-RACE. Sequence analysis showed that the open reading frame encodes a peptide of 372 amino acids and has 50-70% identity with FatA from other plants. Real-time quantitative PCR analysis revealed that AhFatA was expres...
Data
Supplementary Figure 1: Sequence analysis of AhFatA and any other plants. (A) Amino acid sequence alignment of AhFatA and the FatA sequences of other plants. The identical amino acids were showed in white with black background and the conserved amino acids were showed in white and gray background. The three conserved residues are indicated by arrow...
Article
In our early experiments, a variety of Bupleurum scorzonerifolium-like somatic hybrid plants were obtained from protoplast fusion between Arabidopsis thaliana and UV-treated/untreated B. scorzonerifolium. To compare the effects of UV and γ-ray irradiation on the B. scorzonerifolium partner and obtain Arabidopsis-like hybrids, we designed a novel co...
Article
Full-text available
The protoplasts of tall fescue (Festuca arundinacea Schreb.) were fused with those of Bupleurum scorzonerifolium Willd. The latter were irradiated with UV at an intensity of 380 μW/cm2 for 0 s (combination I), 30 s (combination II), and 60 s (combination III) before fusion. Putative hybrid calli, leaves, and shoots were generated from the fusion pr...
Article
Full-text available
The effect of drought and salinity stress on the seedlings of the somatic hybrid wheat cv. Shanrong No. 3 (SR3) and its parent bread wheat cv. Jinan 177 (JN177) was investigated using two-dimensional gel electrophoresis and mass spectrometry. Of a set of 93 (root) and 65 (leaf) differentially expressed proteins (DEPs), 34 (root) and six (leaf) DEPs...
Article
Full-text available
In order to investigate chromosome elimination in symmetric somatic hybridization between Bupleurum scorzonerifolium and Arabidopsis thaliana, protoplasts were isolated from suspension cultures of both A.thaliana and B.scorzonerifolium parents. Biparental protoplasts were mixed at a rate of 1.5:1 and fused with PEG-method. After protoplast fusion,...
Article
The protoplasts of Arabidopsis thaliana (L.) Heynh. treated with 7.5 mM of IOA (iodoacetoamide) were fused with those of Bupleurum scorzonerifolium Willd, using PEG-mediated method. The protoplasts of B. scorzonerifolium were irradiated with ultraviolet light (UV) at an intensity of 380 μW/cm2 for 30, 60, 90, and 120 s, respectively, before fusion....

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