Zev Leifer

Zev Leifer
The Leifer Institute for Molecular and Digital Pathology

Ph.D.

About

38
Publications
11,759
Reads
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377
Citations
Additional affiliations
June 2019 - present
The Leifer Institute for Molecular and Digital Pathology
Position
  • CEO
Description
  • Collaborative Projects in multiple areas of Molecular Pathology (Genetic Regulation) and Pathology Education (Wiki, Paper 3D, Virtual Pathology Lab, Foldscope).
September 1982 - June 2019
New York College of Podiatric Medicine
Position
  • Professor Emeritus
Description
  • Research in Environmental Genotoxicology Assay Development. Digital Pathology Education
September 1976 - July 1982
New York Medical College
Position
  • Research Asst/Associate Professor
Description
  • Research in Environmental Genotoxicology. Ames Assay for mutagens. Pol A Assay for DNA damaging agents. Test development. Metadata analysis for EPA, IARC and ICPEMC.
Education
September 1968 - June 1972
New York University
Field of study
  • Microbiology
September 1963 - June 1965
Harvard University
Field of study
  • Medical Sciences/Biochemistry
September 1959 - June 1963
Yeshiva University
Field of study
  • Biology/Chemistry

Publications

Publications (38)
Article
The detection of DNA-damaging agents by repair-deficient bacterial assays is based on the differential inhibition of growth of repair-proficient and repair-deficient bacterial pairs. The various methodologies used are described and recommendations are made for their improved use. In a survey of the literature through April 1979, 91 of 276 papers ev...
Article
Full-text available
Background: The pathology laboratory course at the New York College of Podiatric Medicine involves the use of Virtual Microscopy. The students can scan the whole slide, section by section, and zoom in or out. Methods: Using the advantages of digital pathology, the students can, in addition, access the slide collections from other medical schools an...
Poster
Full-text available
DNA can assume many unusual local conformational variations, over and above the canonical Watson-Crick double helix. One such structure is the G-quadruplex (G4), formed from a single strand of DNA that contains tracts of guanines (G) that form quartets and stacks of quartets. They have been well-studied in mammalian systems and are notably located...
Poster
Full-text available
This poster describes studies testing the applicability of the Foldscope in the analysis of crystals in liquid as preparation for further work in urinalysis. This would be especially useful in countries or institutions where more expensive microscopes would be prohibitive.
Poster
Full-text available
This poster is part of an ongoing study of small molecule ligands that affect DNA structure and the effect of that change on protein binding. This date will be included in the author's wiki: Small Molecules and DNA Structure http://ligandsanddna.wikifoundry.com/
Conference Paper
Content: There are many needs for slide collections. These include undergraduate and post-graduate medical training, Tumor Board practice, resident review and so on. There are numerous collections of slides, at particular institutions, each with their own particular method of searching and organizing. There is a need for a central site to enable su...
Poster
Full-text available
This poster describes a website designed to tap provide a database (sortable, searchable) with access to digital slides of histopathological tissue. Suitable for teaching and review.
Poster
Full-text available
Describes a wiki that was constructed to present the interaction of small molecules with DNA at the primary, secondary and tertiary structure level. The possible effect on the binding of regulatory proteins is discussed.
Poster
Full-text available
A fascinating story reviewing the literature regarding this unusual amino acid that connects biochemistry, genetics and neuropathology.
Conference Paper
Full-text available
Background: There has been much interest recently in the use of Virtual Patients in Medical Education and Assessment. Clinical scenarios are presented – patient history, examination and test results, treatment decisions and outcomes. The essence of the concept, with appropriate programming, is to vary the options at multiple decision-points, such t...
Article
Full-text available
The pathology laboratory course at the New York College of Podiatric Medicine involves the use of Virtual Microscopy. The students can scan the whole slide, section by section, and zoom in or out. Using the advantages of digital pathology, the students can, in addition, access the slide collections from other medical schools and put up normal histo...
Article
This article introduces the podiatric physician interested in pediatrics to the resources available on the Internet. It surveys search engines, gateway sites on the World Wide Web leading to a wealth of pediatric information and services, and features such as electronic mail, news-groups, and Gopher sites. Examples illustrate how such resources can...
Article
The Independent Studies Program was initiated at the New York College of Podiatric Medicine during the early months of 1987, and it has achieved continued success and acceptance from the administration, faculty, and students. The program emphasizes competence as the constant within a relatively time-independent framework. This paper presents, in a...
Article
Cells of Escherichia coli grown under physiological (noninducing) conditions have a low level of lysine decarboxylase activity. This activity differs from the enzyme found in induced cells in its sensitivity to putrescine (33% of control in the presence of 20 mM putrescine). It is also sensitive to spermidine (20% of control in the presence of 6 mM...
Chapter
Microbial assays are gaining acceptance as valuable procedures for the recognition of environmental chemicals possessing the potential for inducing cancer. Recent studies have shown that assays based upon the preferential inhibition of DNA repair- deficient bacteria, both as adjuncts to other assays (such as the Salmonella mutagenicity procedure) a...
Article
A procedure is described for determining the preferential killing of DNA repair-deficient E. coli. Dilute suspensions of DNA repair-proficient and -deficient bacteria are exposed to a graded series of test chemical concentrations. Survivors are determined by enumeration on agar plates. An expression used to express preferential killing (or lack the...
Chapter
Microbial mutagenicity assay procedures have received considerable attention recently because of the realization that many chemical carcinogens derive their biological activity from their ability to react chemically with cellular DNA. Since mutagens also derive their capacity to induce hereditary changes from their reactivity with cellular DNA, it...
Article
Full-text available
3,4-Dihydroxybutyl-1-phosphonate (DHBP), an analogue of glycerol 3-phosphate, is actively transported by the sn-glycerol 3-phosphate transport system of Escherichia coli strain 8. The Km for the transport of DHBP is 200 microM.
Thesis
Typescript. Thesis (Ph. D.)--New York University, Graduate School of Arts and Science, 1972. Includes bibliographical references.
Article
Full-text available
A mutant of Escherichia coli is described which is defective in the conversion of arginine to putrescine. The activity of the enzyme agmatine ureohydrolase is greatly reduced, whereas the activity of the other two enzymes of the pathway, the constitutive arginine decarboxylase and the inducible arginine decarboxylase, are within the normal range. T...

Questions

Questions (7)
Question
See "Melanomas and Mechanical Stress Points on the Plantar Surface of the Foot". Minagawa, A., Omodaka, T. and Okuyama, R. New Engl J Med 374:24 (June 16, 2016).
Question
How does this relate to parakeratosis in wart - virally infected cells. Same observation, different trigger?
Question
Is there a common mechanism or advantage in both cases? What is the biological relationship?
Question
Ideally, what is known at the molecular/genetic level, transcription factors, etc. See next question.
Question
Ideally, what is known at the molecular/genetic level, transcription factors, etc. See next question.
Question
I would like to know more about unusual secondary structures in DNA (cruciform, triple stranded, G4 and so on) with respect to the effect of these structures on the binding of regulatory proteins. Binding specificity? Binding blockage? Physiological consequences?
Question
I am interested in the effect of small molecules on the three dimensional structure of DNA. I would appreciate any references or researchers/laboratories involved in this area. Which molecules have such an effect? What exactly is the effect? Which level of DNA structure is affected? Please no mutagens or DNA damaging agents.

Network

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Projects

Projects (6)