Zeng-Qiang Qian

Shaanxi Normal University | SNNU · College of Life Sciences

The number of queens per colony and the number of matings per queen are the most important determinants of the genetic structure of ant colonies, and understanding their interrelationship is essential to the study of social evolution. The polygyny-vs.-polyandry hypothesis argues that polygyny and polyandry should be negatively associated because bo...

Eusocial insects vary significantly in colony queen number and mating frequency, resulting in a wide range of social structures. Detailed studies of colony genetic structure are essential to elucidate how various factors affect the relatedness and the sociogenetic organization of colonies. In this study, we investigated the colony structure of the...

Ants are among the most widespread and damaging of invasive alien species. Here, we report the complete mitochondrial genomes for two globally invasive ants: the Argentine ant Linepithema humile and the little fire ant Wasmannia auropunctata. The circular genomes of L. humile and W. auropunctata are 15,929 and 16,362 bp in length, respectively, and...

Anopheles mosquitoes are obligatory vectors for malaria, and their accurate identification is crucial for implementing biological control and malaria prevention. Mitochondrial DNA markers have proven powerful for such purposes. In this study, ten complete and two partial mitochondrial genomes were assembled for a total of 12 Anopheles mosquitoes. T...

Invasive ants are some of the most destructive species in ecosystems and can have serious ecological and socioeconomic impacts. The little fire ant, Wasmannia auropunctata, is native to Central and South America and was listed as one of the 100 most threatening major invasive organisms in the world by the International Union for Conservation of Nat...

Aims Shifts in the realized niches of exotic species may play an important role in their invasion. Galinsoga quadriradiata has invaded China widely and occupied many climate zones that are different from its native range. We addressed the climatic niche shift of G. quadriradiata, and evaluated how this could contribute to its invasion in China. Me...

Akebia trifoliata (Thunb.) Koidz., Akebia trifoliata subsp. australis (Diels) T. Shimizu and Akebia quinata (Houtt.) Decne. are the source plants of the traditional Chinese medicines AKEBIAE CAULIS and AKEBIAE FRUCTUS, and have high pharmaceutical value. However, the resource reserve of these plants has dramatically declined due to habitat destruct...

China is a second center of oak diversity but with less intensively systematic studies. Here, with 49 species representing all four sections in China, we firstly gave insight into the comprehensive phylogenetic relationships of Chinese oaks based on 54 complete plastid genomes. Our results recovered a robust phylogenetic framework and provided stro...

Five Larix species (L. griffithii, L. speciose, L. himalaica, L. kongboensis, and L. potaninii var. australis), have survived on the Qinghai-Tibet Plateau (QTP) under specific climate conditions for decades. The lack of genomic information seriously hinders research on the evolution, conservation and ecology of these Larix resources. In this study,...

Climate change considerably affects vegetation growth and may lead to changes in vegetation distribution. Leopard-skin camphor is an endangered species, and the main raw material for hawk tea, and has various pharmacodynamic functions. Studying the potential distribution of two leopard-skin camphor varieties under climate change should assist in th...

Plants' pattern of compensatory growth is often used to intuitively estimate their grazing tolerance. However, this tolerance is sometimes measured by the overall grazing tolerance index (overall GTI), which assumes that tolerance is a multivariate linear function of various underlying mechanisms. Because the interaction among mechanisms is not ind...

Damselflies and dragonflies, of the order Odonata, have distinct body plans and predatory abilities. Knowledge of their various evolutionary histories will allow for an understanding of the genetic and phenotypic evolution of insects. Mitogenomes are suitable materials to elucidate this, but the mitogenome of only a few odonates have been annotated...

Complete chloroplast genomes were characterized for two economically important tree species, Cinnamomum camphora and Cinnamomum parthenoxylon. The chloroplast genomes are 152,721 bp (C. camphora) and 152,760 bp (C. parthenoxylon) long, respectively, and both comprise a pair of inverted repeat (IR) regions, separated by a large single-copy (LSC) reg...

Anopheles funestus is a major malaria vector in Africa. In this study, its complete mitochondrial genome was assembled from high-throughput sequencing reads. The circular genome is 15,403 bp long with an A + T-biased base composition and harbors 37 genes (including 13 protein-coding genes/PCGs, 22 tRNAs, and two rRNAs) and one control region. The P...

Cardiocondyla obscurior is native to Southeast Asia but has become successfully established throughout the tropics and subtropics. Here, its mitochondrial genome was assembled from Illumina sequencing data. The genome is 15,213 bp long with a highly asymmetric nucleotide composition, and harbors the typical set of 37 mitochondrial genes and one non...

The mole salamander Ambystoma talpoideum is native to southeastern and central United States, and is listed as a threatened or endangered species by local governments due to anthropogenic disturbance. Here, its mitochondrial genome was characterized from Illumina sequencing reads. The genome is 16,358 bp long with an asymmetric nucleotide compositi...

Eulophia zollingeri (Orchidaceae) is a vulnerable plant in China. In this study, the complete chloroplast genome of E. zollingeri is assembled based on high-throughput sequencing data for the first time. The complete chloroplast of E. zollingeri is 145,201 bp in length, containing a large single copy (LSC) of 81,566 bp, a small single copy of 13,09...

Galinsoga quadriradiata is an invasive weed in many parts of the world, and are causing serious damages to local biodiversity and agriculture. In this study, its complete chloroplast genome was assembled from the whole-genome Illumina sequencing data. The circular genome is 151,917 bp long, and comprises a pair of inverted repeat regions (IRs, 25,0...

The East Himalaya-Hengduan Mountains (EH-HM) region has a high biodiversity and harbours numerous endemic alpine plants. This is probably the result of combined orographic and climate oscillations occurring since late Tertiary. Here, we determined the genetic structure and evolutionary history of alpine oak species (including Q. spinosa, Q. aquifol...

Mantel tests between microsatellite-based genetic distances and geographic distances. (A) Quercus spinosa; (B) Q. aquifolioides; (C) Q. rehderiana.

The variable sites in aligned nrDNA sequences detected in the ITS4-ITS5 region of the three related species from 33 populations, which identified 14 haplotypes (N1–N14).

Prior distributions for model parameters used in model comparisons.

(A) Network of the ITS haplotypes detected in three oak species. Different species are denoted by different colors of the circle, each sector of a circle is in proportional to the frequency of each haplotype. (B) Geographic distribution of the ITS haplotypes detected in three oak species. Haplotype frequencies of each population are denoted by the...

Sampling details and haplotypes detected in each population of the three related species.

Genetic diversity parameters estimated at 12 microsatellite loci in 33 populations.

Ecological niche models predicted distributions of the three species using GARP during three periods. Different colors corresponded to different fitting indices with low in blue and high in red for the current, LGM and LIG distribution.

Bottleneck analysis for 33 populations of the three related species.

Description of the oak species and their habitats in our study.

Bayesian inference analysis of microsatellite data for determining the most likely number of clusters (K) for the three lineages of oak species occurred in the EH-HM region. The distribution of the likelihood L(K) values (A) and ΔK values (B) are presented for K = 1–15 (10 replicates). STRUCTURE plots (C) are presented for best K = 3 (QS1: populati...

The variable sites in aligned cpDNA sequences of psbA-trnH, psbB-psbF, and matK that yielded 25 chlorotypes (C1–C25) recorded across the three species from 34 populations.

The nine scenarios for four lineages.

The SSR dataset of these three oak species in this study.

Methodological details for sequences and microsatellite genotyping.

The 19 bioclimatic variables used in the full set of variables, with √ indicating the variables used in the customized sets for Q. spinosa (QS), Q. rehderiana (QR), and Q. aquifolioides (QA); the variables used in the reduced set.

Posterior median estimate and 95% highest posterior density interval (HPDI) for demographic parameters of an contraction-expansion model based on the nuclear multilocus microsatellite data of three oak species, respectively.

The complete mitogenome of the Cydno Longwing Heliconius cydno has been reconstructed from the whole-genome Illumina sequencing data. The circular genome is 15,367 bp in length, and consists of 13 protein-coding genes (PCGs), 22 transfer RNAs (tRNAs), 2 ribosomal RNAs (rRNAs) and 1 D-loop region. PCGs are mostly initiated with the ATN codons (COII,...

The complete mitogenome of the dampwood termite Zootermopsis nevadensis was reconstructed from whole-genome Illumina sequencing data with an average coverage of 7052×. The circular genome is 15,444 bp in length, and consists of 22 transfer RNAs (tRNAs), 13 protein-coding genes (PCGs), two ribosomal RNAs (rRNAs) and one D-loop region. All PCGs are i...

The complete chloroplast genome (cpDNA) sequence of an endemic conifer species, Armand pine Pinus armandii Franch., is determined in this study. The cpDNA was 117,265 bp in length, containing a pair of 475 bp inverted repeat (IR) regions those distinguished in large and small single copy (LSC and SSC) regions of 64,548 and 51,767 bp in length, resp...

The myrmicine ant Vollenhovia emeryi is native to East Asia, but has recently become established in North America. In this study, its complete mitochondrial genome has been assembled from Illumina Miseq sequencing data with a good coverage (22.5X). The circular genome is 16,259 bp in length, and harbors 13 protein-coding genes (PCGs), 22 tRNA genes...

The complete mitochondrial genome of the Sara Longwing Heliconius sara has been reconstructed from the whole-genome Illumina sequencing data. The mitochondrial genome is 15,372 bp in size with the highly asymmetric overall A + T content of 80.6%. Annotation of mitochondrial genome revealed a total of 37 genes, including 13 protein-coding genes (PCG...

Abstract The complete mitochondrial genome of Formica selysi has been assembled from Illumina sequencing data with an average coverage of 2733X. The circular genome was 16,752 bp in length, and consists of 13 protein-coding genes (PCGs), 22 transfer RNAs (tRNAs), two ribosomal RNAs (rRNAs) and one D-loop region. All PCGs initiated with ATN codons a...

Abstract The complete mitochondrial genome of the wild silk moth Bombyx huttoni has been reconstructed from the whole-genome Illumina sequencing data. The circular genome was determined to be 15,638 bp in length, consisting of 13 protein-coding genes (PCGs), 22 transfer RNAs (tRNAs), two ribosomal RNAs (rRNAs), and one A+T-rich D-loop or control re...

Euphorbia kansui is a threatened perennial herb endemic to China, and a good knowledge of its population genetics would be essential to the formulation of effective conservation and management strategies. In this study, 12 novel microsatellite markers were developed for E. kansui using Illumina sequencing technology. The number of alleles varied be...

Cupressus gigantea Cheng et L. K. Fu is an endangered conifer endemic to Tibet of China. In this study, 16 polymorphic microsatellite markers were developed using paired-end Illumina shotgun sequencing. The number of alleles varied between 3 and 18, with an average of 8.063 alleles per locus. The observed (H O) and expected heterozygosities (H E) r...

Speciation has long been a core issue in evolutionary biology and is of fundamental importance to the formation of biodiversity. The traditional view of allopatric speciation holds that geographic isolation is a major driver of speciation and that species divergence only occurs in circumstances where there is geographic isolation between population...

The ant genus Myrmecia Fabricius (Hymenoptera: Formicidae) is endemic to Australia and New Caledonia, and has retained many biological traits that are considered to be basal in the family Formicidae. Here, a set of 16 dinucleotide microsatellite loci were studied that are polymorphic in at least one of the two species of the genus: the giant bulldo...

Saruma henryi Oliv. (Aristolochiaceae) is an endangered herb endemic to China. In this study, chloroplast microsatellites (cpSSRs) and sequences of the atpB-rbcL intergenic spacers were employed to reveal its genetic diversity and phylogeographic patterns. We detected high within-species genetic diversity (H(T)=0.939 for cpSSR; H(T)=0.862 for atpB-...

Saruma henryi Oliv., the only representative of the monotypic genus Saruma Oliv. (Aristolochiaceae), is an endangered perennial herb endemic to China. It is a phylogenetically, ecologically, and medicinally important species. In the present study, inter-simple sequence repeat (ISSR) markers were employed to investigate the genetic diversity and dif...

Saruma henryi Oliv. (Aristolochiaceae) is an endangered perennial herb endemic to China, and has received considerable attention for its high phylogenetic, ecological and medicinal significances. To investigate its genetic diversity and structure, 14 polymorphic microsatellite loci were isolated and characterized from a dinucleotide-enriched genomi...

Saruma henryi Oliv. is an endangered perennial herb endemic to China, and possesses high phylogenetic, ecological and medicinal values. In this study, 12 polymorphic microsatellite markers were developed from publicly available expressed sequence tags (ESTs) of S. henryi. Analysis of 24 individuals from a single population showed that the number of...

The fire ant Solenopsis invicta has received considerable attention as a damaging invasive pest. In the present study, 12 polymorphic microsatellite loci were developed from publicly available expressed sequence tags (ESTs) of S. invicta. The number of alleles varied between 3 and 12, with an average of 6.42 per locus. The observed and expected het...

In this study, the genetic diversity and differentiation of 10 natural Prunus pseudocerasus Lindl. populations were investigated using inter-simple sequence repeat (ISSR) markers. Totally, 18 selected primers generated 150 loci, with an average of 8.33 bands per primer. The results showed that the percentage of polymorphic bands (PPB) was pretty lo...

The genus Gynostemma (Cucurbitaceae) constitutes a polyploid group of perennial creeping herbs, in whose evolution polyploidization is a key component. With the largest variety of cytotypes (2n=22, 44, 66 and 88) in Gynostemma, G. pentaphyllum is also the most widespread species in this genus. In the present study, we inferred the origins of polypl...

The genetic diversity of 17 populations of Clintonia udensis Trautv. et Mey. was studied by using 15 intersimple sequence repeat primers. The results revealed a relatively high genetic diversity, with percentage of polymorphic bands = 98.8%, Shannon's information index = 0.56, and Nei's gene diversity index = 0.38. The genetic diversity at the popu...

Studies were performed to investigate the genetic variation of 14 natural populations of Gynostemma pentaphyllum (Thunb.) Makino, an outcrossing clonal plant species in China, using inter-simple sequence repeat (ISSR) markers. Fourteen selected primers were used to amplify DNA samples from 140 individuals, and totally 194 loci were detected. The pe...

Using inter-simple sequence repeat (ISSR) markers, studies were performed to characterize the population genetic diversity and structure of Reaumuria soongorica (Pall.) Maxim. in the oasis-desert ecotone in Fukang, Xinjiang. Eleven primers were screened to amplify DNA sequences from 132 individuals, which corresponded to seven subpopulations. Total...

The genus Dipteronia Oliv. endemic to central and southern China consists of two species, Dipteronia sinensis Oliv. and Dipteronia dyeriana Henry, both of them are rare and endangered. AFLP markers were used to characterize the genetic diversity and geographical differentiation of the genus. Eight out of 32 PstI + 3/MseI + 3 selective primer combin...

Larix potaninii var. chinensis is endemic to China and is found only on several peaks of the Qinling Mountains in Shaanxi Province. In China, it is classified in the second class of national protected rare plants. To estimate genetic diversity and to analyze population genetic structure of L. potaninii var. chinensis, 120 individual samples from si...

Dipteronia is an endemic genus to China and includes only two species, Dipteronia sinensis and D. dyeriana. Based on random amplified polymorphic DNA (RAPD) markers, a comparative study of the genetic diversity and genetic structure of Dipteronia was performed. In total, 128 and 103 loci were detected in 17 D. sinensis populations and 4 D. dyeriana...

Isozyme polymorphism of 40 Haloxylon ammodendron individuals sampled from the oasis-desert ecotone in Fukang, Xinjiang was preliminarily studied by using discontinuous polyacrylamide gel electrophoresis (PAGE) technique. 14 were obtained from 24 enzymatic systems for the analysis of genetic variation. Totally 30 coding loci, corresponding to 54 all...

Variations in malondiadehycle (MDA) contents, enzymatic activities and isozymatic patterns of superoxide dismutases (SOD), peroxidases (POD) and catalases (CAT) of leaves at four-leaf stage were studied by treating Vicia faba L. with 3.50mW·mm-2 He-Ne laser at different stages. The results indicated that He-Ne laser lead to a significant decrease i...

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