Zeljka Krpetic

Zeljka Krpetic
University of Salford · School of Science Engineering and Environment

MChem, PhD

About

33
Publications
7,560
Reads
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1,877
Citations
Citations since 2017
7 Research Items
1108 Citations
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2017201820192020202120222023050100150200
2017201820192020202120222023050100150200
2017201820192020202120222023050100150200
Introduction
Lecturer in Physical Chemistry in the School of Science Engineering and Environment Programme Leader Degree Apprenticeship in Chemical Sciences PI and group leader: www.zknanolab.com and Associate Editor for Springer-Nature's Cancer Nanotechnology
Additional affiliations
October 2012 - present
University College Dublin
Position
  • Senior Researcher
July 2011 - October 2012
University of Liverpool
Position
  • Research Associate
May 2010 - July 2011
University of Strathclyde

Publications

Publications (33)
Article
Full-text available
We describe the outcome of a large international interlaboratory study of the measurement of particle number concentration of colloidal nanoparticles, project 10 of the technical working area 34, "Nanoparticle Populations" of the Versailles Project on Advanced Materials and Standards (VAMAS). A total of 50 laboratories delivered results for the num...
Article
Full-text available
Advances in nanofabrication methods have enabled the tailoring of new strategies towards the controlled production of nanoparticles with attractive applications in healthcare. In many cases, their characterisation remains a big challenge, particularly for small-sized functional nanoparticles of 5 nm diameter or smaller, where current particle sizin...
Article
Full-text available
The range of possible nanostructures is so large and continuously growing, that collating and unifying the knowledge connected to them, including their biological activity, is a major challenge. Here we discuss a concept that is based on the connection of microscopic features of the nanomaterials to their biological impacts. We also consider what w...
Article
The transport and the delivery of drugs through nanocarriers is a great challenge of pharmacology. Since the production of liposomes to reduce the toxicity of doxorubicin in patients, a plethora of nanomaterials have been produced and characterized. Although it is widely known that elementary properties of nanomaterials influence their in vivo kine...
Article
Full-text available
The shape and size of nanoparticles are important parameters affecting the biodistribution, bioactivity, and toxicity. The high-throughput characterisation of nanoparticle shape in the dispersion is a fundamental prerequisite for realistic in vitro and in vivo evaluation, however, with routinely available bench-top optical characterisation techniqu...
Article
Nanoparticles in a biological milieu are known to form a sufficiently long-lived and well-organized 'corona' of biomolecules to confer a biological identity to the particle. Because this nanoparticle-biomolecule complex interacts with cells and biological barriers, potentially engaging with different biological pathways, it is important to clarify...
Article
The standard protocols for DNA analysis largely involve polymerase chain reaction (PCR). However, DNA structures bound to chemical agents cannot be PCR-amplified and therefore any sequence changes induced by external agents may remain neglected. Thus, the development of analytical tools capable of characterizing the biochemical mecha-nisms associat...
Article
The exploring of the genetic information carried by DNA has become a major scientific challenge. Routine DNA analysis, such as PCR, still suffers from intrinsic important limitations. Surface-enhanced Raman spectroscopy (SERS) has emerged as an outstanding opportunity for the development of DNA analysis but the application to duplexes (dsDNA) has b...
Article
It is demonstrated that a compact monolayer of 1,2-dioleoyl-sn-glycero-3-phosphocholine (DOPC) adsorbed to a hanging mercury drop electrode (HMDE) can serve as a simple electrochemical model system to study bio-membrane penetration by gold nanoparticles. The hydrogen redox-chemistry characteristic of ligand-stabilized gold nanoparticles in molecula...
Article
Colloidal nanoparticles designed for the interactions with cells are very small, nanoscale objects usually consisting of inorganic cores and organic shells that are dispersed in a buffer or biological medium. By tuning the material properties of the nanoparticles a number of different biological applications of nanomaterials are enabled i.e. target...
Article
Full-text available
The preparation of 3-4 nm and 10 nm gold nanoparticles capped with neutral carborane-based mercaptocarboranes, via two different preparative routes, is reported. The resulting boron-enriched nanomaterials exhibit complete dispersibility in water, opening the way for the use of these monolayer protected clusters (MPCs) in medical applications, such...
Article
Differential centrifugal sedimentation (DCS) has been applied to accurately size ligand-protected gold hydrosols in the 10 to 50 nm range. A simple protocol is presented to correct for particle density variations due to the presence of the ligand shell, which is formed here by either polyethylene glycol substituted alkane thiols (PEG-alkane thiols)...
Article
Full-text available
Since alternative splicing patterns are exhibited by most mammalian genes, the functional properties of a gene may be changed for therapeutic purposes by nudging splicing from one pattern to another. Splicing to a refractory exon can be enhanced using bifunctional targeted oligonucleotide enhancers of splicing (TOES), which anneal to a target exon...
Article
Traditional methods for the construction of nanoparticle arrays and lattices exploit Watson-Crick base pairing of single-stranded DNA sequences as a proxy for self-assembly. Although this approach has been utilized in a variety of applications in nanoassembly, diagnostics, and biomedicine, the diversity of this recognition lexicon could be consider...
Article
Full-text available
Improved positively charged nanoparticles are described to provide a simplified SERS substrate for DNA detection. Complete flocculation of the nanoparticles is prevented due to the controlled analyte induced aggregation. This provides a stable aggregation state which significantly extends the analysis window simplifying DNA detection by SERS.
Article
Colorimetric detection of analytes using gold nanoparticles along with surface-enhanced Raman spectroscopy (SERS) are areas of intense research activity since they both offer sensing of very low concentrations of target species. Multimodal detection promotes the simultaneous detection of a sample by a combination of different techniques; consequent...
Article
A simple single-phase method for the preparation of ca. 2 nm gold nanoparticles capped with mercaptocarborane ligands is introduced. The resultant monolayer protected clusters (MPCs) exhibit redox-dependent solubility and readily phase transfer between water and nonpolar solvents depending on the electronic and ionic charge stored in the metal core...
Article
Gold nanoparticles (GNPs) are of considerable interest for use as a radiosensitizer, because of their biocompatibility and their ability to increase dose deposited because of their high mass energy absorption coefficient. Their sensitizing properties have been verified experimentally, but a discrepancy between the experimental results and theoretic...
Article
Here we report on the design, synthesis and application of small, highly bright, star-shaped SERS encoded single nanoparticles with the ability of providing an optical signal upon excitation with near infrared light. These particles are colloidally stable, fully biocompatible and can be internalized into living cells for intracellular imaging.
Article
Uptake of PEGylated gold nanoparticles by HeLa cells in the presence of acrylate is described. Nanoparticles that are usually not taken up readily cross the plasma membrane into the cytosol in the presence of acrylate. In addition, a method is described that allows the introduction of large amounts of pre-aggregated gold nanoparticles into the cell...
Article
Full-text available
This paper contributes to the debate on how nanosized objects negotiate membrane barriers inside biological cells. The uptake of peptide-modified gold nanoparticles by HeLa cells has been quantified using atomic emission spectroscopy. The TAT peptide from the HIV virus was singled out as a particularly effective promoter of cellular uptake. The evo...
Article
We show that low-intensity laser irradiation of cancer cells containing endosomal gold nanoparticles leads to endosome rupture and escape of the nanoparticles into the cytosol without affecting the cells' viability. The low light intensity of our experiments allows us to rule out photothermal effects as the underlying mechanism, and we present resu...
Article
Full-text available
We report the evidence for the cellular uptake of gold nanoparticles via the phagocytosis mechanism in murine macrophage cells strongly supported by TEM and optical microscopy. Nanoparticles were prepared using several biocompatible molecules of choice (5-aminovaleric acid, l-DOPA, melatonin, and serotonin hydrochloride) as stabilizers for gold col...
Article
A novel use of two components of Cape aloe, aloin A and aloesin, acting as stabilizers in the preparation of gold and silver nanoparticles, is reported. Stable water-soluble particles of different size and shape are prepared by varying the reaction conditions, temperature, reaction time, and reducing agents. Characterization of the obtained particl...
Article
Gold nanoparticles of two different sizes stabilized by a 15-mer peptide ligand specifically designed for this purpose have been prepared in aqueous solution and characterized by UV-vis spectroscopy and TEM. The presence of the ligand and its binding mode to the particles via its four cystein thiols is evidenced by FTIR and NMR spectroscopy. Biotin...
Article
Novel reproducible preparations of gold nanoparticles capped by aminoalcohols have been set up by reduction of HAuCl4 with NaBH4 in aqueous solution. The characterization of Au@aminoalcohol nanoparticles performed by 1H NMR, ATR-FTIR, UV-vis spectroscopies, and TEM microscopy highlighted the binding site and the nature of the Au-NH bond.

Questions

Question (1)
Question
I know that there is a paper out there somewhere, but I forgot the authors' names and somehow I can not find it anymore.

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Projects

Projects (2)
Archived project
The goal of this project was to investigate the application of gold nanoparticles in biofuels and forensics.
Archived project
My MSc research project (60 credits), involved the synthesis and characterisation of functionalised gold nanoparticle behaviour in biological media as a first step towards future clinical trials involving nanomedicine. During this project I synthesised different sized gold nanoparticles and functionalised them with various chemical ligands. I also characterised the behaviour and stability of my systems by UV-Vis spectroscopy and gel electrophoresis when incubated with biological media commonly used for bacteria and cell cultures. I achieved the highest mark in my cohort for my work.